ID   BADH_SPIOL              Reviewed;         497 AA.
AC   P17202; P93555;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   16-JUN-2009, entry version 68.
DE   RecName: Full=Betaine aldehyde dehydrogenase, chloroplastic;
DE            Short=BADH;
DE            EC=1.2.1.8;
DE   Flags: Precursor;
OS   Spinacia oleracea (Spinach).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   Caryophyllales; Amaranthaceae; Spinacia.
OX   NCBI_TaxID=3562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=cv. Savoy hybrid 612;
RX   MEDLINE=90207274; PubMed=2320587; DOI=10.1073/pnas.87.7.2745;
RA   Weretilnyk E.A., Hanson A.D.;
RT   "Molecular cloning of a plant betaine-aldehyde dehydrogenase, an
RT   enzyme implicated in adaptation to salinity and drought.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:2745-2749(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Shu W., Ai W., Chen S.;
RL   Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: Betaine aldehyde + NAD(+) + H(2)O = betaine +
CC       NADH.
CC   -!- PATHWAY: Amine and polyamine biosynthesis; betaine biosynthesis
CC       via choline pathway; betaine from betaine aldehyde: step 1/1.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR   EMBL; M31480; AAA34025.1; -; mRNA.
DR   EMBL; U69142; AAB41696.1; -; Genomic_DNA.
DR   PIR; A35994; A35994.
DR   PIR; T51173; T51173.
DR   HSSP; P51977; 1BXS.
DR   BRENDA; 1.2.1.8; 286.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0008802; F:betaine-aldehyde dehydrogenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR016160; Ald_DH_CS.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH.
DR   Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; NAD; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT       1      7       Chloroplast (Potential).
FT   CHAIN         8    497       Betaine aldehyde dehydrogenase,
FT                                chloroplastic.
FT                                /FTId=PRO_0000007182.
FT   NP_BIND     235    240       NAD (By similarity).
FT   ACT_SITE    257    257       Proton acceptor (By similarity).
FT   ACT_SITE    291    291       Nucleophile (By similarity).
FT   SITE        159    159       Transition state stabilizer (By
FT                                similarity).
FT   MOD_RES       8      8       Blocked amino end (Arg).
FT   CONFLICT    424    424       S -> F (in Ref. 2; AAB41696).
SQ   SEQUENCE   497 AA;  54270 MW;  55088240E635B22F CRC64;
     MAFPIPARQL FIDGEWREPI KKNRIPVINP STEEIIGDIP AATAEDVEVA VVAARRAFRR
     NNWSATSGAH RATYLRAIAA KITEKKDHFV KLETIDSGKP FDEAVLDIDD VASCFEYFAG
     QAEALDGKQK APVTLPMERF KSHVLRQPLG VVGLISPWNY PLLMATWKIA PALAAGCTAV
     LKPSELASVT CLEFGEVCNE VGLPPGVLNI LTGLGPDAGA PLVSHPDVDK IAFTGSSATG
     SKVMASAAQL VKPVTLELGG KSPIVVFEDV DIDKVVEWTI FGCFWTNGQI CSATSRLLVH
     ESIAAEFVDK LVKWTKNIKI SDPFEEGCRL GPVISKGQYD KIMKFISTAK SEGATILYGG
     SRPEHLKKGY YIEPTIVTDI STSMQIWKEE VFGPVLCVKT FSSEDEAIAL ANDTEYGLAA
     AVFSNDLERC ERITKALEVG AVWVNCSQPC FVQAPWGGIK RSGFGRELGE WGIQNYLNIK
     QVTQDISDEP WGWYKSP
//