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P17200 (ACM4_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M4
Gene names
Name:CHRM4
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase. May couple to multiple functional responses in cell lines.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in heart and brain.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Muscarinic acetylcholine receptor M4
PRO_0000069040

Regions

Topological domain1 – 4242Extracellular By similarity
Transmembrane43 – 6422Helical; Name=1; By similarity
Topological domain65 – 7814Cytoplasmic By similarity
Transmembrane79 – 9921Helical; Name=2; By similarity
Topological domain100 – 11617Extracellular By similarity
Transmembrane117 – 13822Helical; Name=3; By similarity
Topological domain139 – 15820Cytoplasmic By similarity
Transmembrane159 – 18123Helical; Name=4; By similarity
Topological domain182 – 20322Extracellular By similarity
Transmembrane204 – 22623Helical; Name=5; By similarity
Topological domain227 – 412186Cytoplasmic By similarity
Transmembrane413 – 43321Helical; Name=6; By similarity
Topological domain434 – 44714Extracellular By similarity
Transmembrane448 – 46720Helical; Name=7; By similarity
Topological domain468 – 49023Cytoplasmic By similarity

Amino acid modifications

Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation151N-linked (GlcNAc...) Potential
Glycosylation201N-linked (GlcNAc...) Potential
Glycosylation251N-linked (GlcNAc...) Potential
Disulfide bond115 ↔ 195 By similarity

Sequences

Sequence LengthMass (Da)Tools
P17200 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 2CDFDB5FA7D2298E

FASTA49054,937
        10         20         30         40         50         60 
MHNLSAQPWQ AKMANLTYDN VTLSNRSEVA IQPPTNYKTV ELVFIATVTG SLSLVTVVGN 

        70         80         90        100        110        120 
ILVMLSIKVN RQLQTVNNYF LFSLACADLI IGVFSMNLYT VYIIKGYWPL GAVVCDLWLA 

       130        140        150        160        170        180 
LDYVVSNASV MNLLIISFDR YFCVTKPLTY PARRTTKMAG LMIAAAWILS FILWAPAILF 

       190        200        210        220        230        240 
WQFIVGKRTV HERECYIQFL SNPAVTFGTA IAAFYLPVVI MTVLYIHISL ASRSRVRRHK 

       250        260        270        280        290        300 
PESRKERKGK SLSFFKAPPV KQNNNNSPKR AVEVKEEVRN GKVDDQPSAQ TEATGQQEEK 

       310        320        330        340        350        360 
ETSNESSTVS MTQTTKDKPT TEILPAGQGQ SPAHPRVNPT SKWSKIKIVT KQTGTESVTA 

       370        380        390        400        410        420 
IEIVPAKAGA SDHNSLSNSR PANVARKFAS IARSQVRKKR QMAAREKKVT RTIFAILLAF 

       430        440        450        460        470        480 
ILTWTPYNVM VLINTFCETC VPETVWSIGY WLCYVNSTIN PACYALCNAT FKKTFKHLLM 

       490 
CQYRNIGTAR

« Hide

References

[1]"Cloning and functional analysis of a gene encoding a novel muscarinic acetylcholine receptor expressed in chick heart and brain."
Tietje K.M., Goldman P.S., Nathanson N.M.
J. Biol. Chem. 265:2828-2834(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J05218 Genomic DNA. Translation: AAA48563.1.
IPIIPI00587862.
PIRA35546.
RefSeqNP_001026362.1. NM_001031191.1.
UniGeneGga.32110.

3D structure databases

ProteinModelPortalP17200.
ModBaseSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000013605.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PaxDbP17200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID423195.
KEGGgga:423195.

Organism-specific databases

CTD1132.

Phylogenomic databases

eggNOGNOG250863.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidP17200.
KOK04132.
OrthoDBEOG41JZCC.

Family and domain databases

InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_M4_rcpt.
IPR000995. Musac_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP17200.
NextBio20825697.

Entry information

Entry nameACM4_CHICK
AccessionPrimary (citable) accession number: P17200
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

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