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Protein

Muscarinic acetylcholine receptor M4

Gene

CHRM4

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase. May couple to multiple functional responses in cell lines.

GO - Molecular functioni

  • G-protein coupled acetylcholine receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M4
Gene namesi
Name:CHRM4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4242ExtracellularBy similarityAdd
BLAST
Transmembranei43 – 6422Helical; Name=1By similarityAdd
BLAST
Topological domaini65 – 7814CytoplasmicBy similarityAdd
BLAST
Transmembranei79 – 9921Helical; Name=2By similarityAdd
BLAST
Topological domaini100 – 11617ExtracellularBy similarityAdd
BLAST
Transmembranei117 – 13822Helical; Name=3By similarityAdd
BLAST
Topological domaini139 – 15820CytoplasmicBy similarityAdd
BLAST
Transmembranei159 – 18123Helical; Name=4By similarityAdd
BLAST
Topological domaini182 – 20322ExtracellularBy similarityAdd
BLAST
Transmembranei204 – 22623Helical; Name=5By similarityAdd
BLAST
Topological domaini227 – 412186CytoplasmicBy similarityAdd
BLAST
Transmembranei413 – 43321Helical; Name=6By similarityAdd
BLAST
Topological domaini434 – 44714ExtracellularBy similarityAdd
BLAST
Transmembranei448 – 46720Helical; Name=7By similarityAdd
BLAST
Topological domaini468 – 49023CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Muscarinic acetylcholine receptor M4PRO_0000069040Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi3 – 31N-linked (GlcNAc...)Sequence analysis
Glycosylationi15 – 151N-linked (GlcNAc...)Sequence analysis
Glycosylationi20 – 201N-linked (GlcNAc...)Sequence analysis
Glycosylationi25 – 251N-linked (GlcNAc...)Sequence analysis
Disulfide bondi115 ↔ 195PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP17200.

Expressioni

Tissue specificityi

Expressed in heart and brain.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000013605.

Chemistry

BindingDBiP17200.

Structurei

3D structure databases

ProteinModelPortaliP17200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP17200.
KOiK04132.
PhylomeDBiP17200.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNLSAQPWQ AKMANLTYDN VTLSNRSEVA IQPPTNYKTV ELVFIATVTG
60 70 80 90 100
SLSLVTVVGN ILVMLSIKVN RQLQTVNNYF LFSLACADLI IGVFSMNLYT
110 120 130 140 150
VYIIKGYWPL GAVVCDLWLA LDYVVSNASV MNLLIISFDR YFCVTKPLTY
160 170 180 190 200
PARRTTKMAG LMIAAAWILS FILWAPAILF WQFIVGKRTV HERECYIQFL
210 220 230 240 250
SNPAVTFGTA IAAFYLPVVI MTVLYIHISL ASRSRVRRHK PESRKERKGK
260 270 280 290 300
SLSFFKAPPV KQNNNNSPKR AVEVKEEVRN GKVDDQPSAQ TEATGQQEEK
310 320 330 340 350
ETSNESSTVS MTQTTKDKPT TEILPAGQGQ SPAHPRVNPT SKWSKIKIVT
360 370 380 390 400
KQTGTESVTA IEIVPAKAGA SDHNSLSNSR PANVARKFAS IARSQVRKKR
410 420 430 440 450
QMAAREKKVT RTIFAILLAF ILTWTPYNVM VLINTFCETC VPETVWSIGY
460 470 480 490
WLCYVNSTIN PACYALCNAT FKKTFKHLLM CQYRNIGTAR
Length:490
Mass (Da):54,937
Last modified:August 1, 1990 - v1
Checksum:i2CDFDB5FA7D2298E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05218 Genomic DNA. Translation: AAA48563.1.
PIRiA35546.
RefSeqiNP_001026362.1. NM_001031191.1.
UniGeneiGga.32110.

Genome annotation databases

GeneIDi423195.
KEGGigga:423195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05218 Genomic DNA. Translation: AAA48563.1.
PIRiA35546.
RefSeqiNP_001026362.1. NM_001031191.1.
UniGeneiGga.32110.

3D structure databases

ProteinModelPortaliP17200.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000013605.

Chemistry

BindingDBiP17200.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiP17200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi423195.
KEGGigga:423195.

Organism-specific databases

CTDi1132.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiP17200.
KOiK04132.
PhylomeDBiP17200.

Miscellaneous databases

NextBioi20825697.
PROiP17200.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001432. Musac_Ach_M4_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF60. PTHR24249:SF60. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00541. MUSCRINICM4R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and functional analysis of a gene encoding a novel muscarinic acetylcholine receptor expressed in chick heart and brain."
    Tietje K.M., Goldman P.S., Nathanson N.M.
    J. Biol. Chem. 265:2828-2834(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiACM4_CHICK
AccessioniPrimary (citable) accession number: P17200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.