Reviewed,
UniProtKB/Swiss-Prot P17183 (ENOG_MOUSE)
Last modified
November 25, 2008.
Version 91.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Gamma-enolase EC=4.2.1.11 Alternative name(s): 2-phospho-D-glycerate hydro-lyase Neural enolase Neuron-specific enolase Short name=NSE Enolase 2 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 434 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival By similarity. |
| Catalytic activity | 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O. |
| Cofactor | Magnesium. Required for catalysis and for stabilizing the dimer. |
| Pathway | Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. |
| Subunit structure | Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. |
| Subcellular location | CytoplasmBy similarity. Cell membraneBy similarity. Note= Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form By similarity. |
| Tissue specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
| Developmental stage | During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. |
| Sequence similarities | Belongs to the enolase family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cell membrane Cytoplasm Membrane |
| Ligand | Magnesium Metal-binding |
| Molecular function | Lyase |
| PTM | Phosphoprotein |
| Technical term | Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: InterPro |
| Cellular component | phosphopyruvate hydratase complex Inferred from electronic annotation. Source: InterPro plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphopyruvate hydratase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 434 | 433 | Gamma-enolase | PRO_0000134113 | |||||
Regions | |||||||||
| Region | 370 – 373 | 4 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 210 | 1 | Proton donor By similarity | ||||||
| Active site | 343 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 245 | 1 | Magnesium By similarity | ||||||
| Metal binding | 293 | 1 | Magnesium By similarity | ||||||
| Metal binding | 318 | 1 | Magnesium By similarity | ||||||
| Binding site | 158 | 1 | Substrate By similarity | ||||||
| Binding site | 167 | 1 | Substrate By similarity | ||||||
| Binding site | 293 | 1 | Substrate By similarity | ||||||
| Binding site | 318 | 1 | Substrate By similarity | ||||||
| Binding site | 394 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 25 | 1 | Phosphotyrosine | ||||||
| Modified residue | 44 | 1 | Phosphotyrosine | ||||||
| Modified residue | 80 | 1 | Phosphoserine | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequences of cDNAs alpha and gamma enolase mRNAs from mouse brain." Kaghad M., Dumont X., Chalon P., Lelias J.M., Lamande N., Lucas M., Lazar M., Caput D. Nucleic Acids Res. 18:3638-3638(1990) [PubMed: 2362815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [2] | "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6." Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A. Genome Res. 8:29-40(1998) [PubMed: 9445485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Retina. |
| [4] | Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 16-28; 33-50; 73-89; 104-120; 163-179; 184-193; 203-228; 240-262; 270-285; 307-326; 336-358; 373-394 AND 413-422, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain. |
| [5] | Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 33-50; 73-89; 163-179; 270-285 AND 423-429, MASS SPECTROMETRY. Tissue: Hippocampus. |
| [6] | "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L., Hart G.W., Burlingame A.L. Proteomics 5:388-398(2005) [PubMed: 15648052] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80, MASS SPECTROMETRY. Tissue: Brain. |
| [7] | "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-25 AND TYR-44, MASS SPECTROMETRY. Tissue: Mast cell. |
| [8] | "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-25 AND TYR-44, MASS SPECTROMETRY. Tissue: Brain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X52380 mRNA. Translation: CAA36606.1. AC002397 Genomic DNA. Translation: AAC36002.1. BC031739 mRNA. Translation: AAH31739.1. | |
| PIR | S10247. |
| RefSeq | NP_038537.1. |
| UniGene | Mm.3913 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1PDZ based on UniProtKB P56252. |
| SMR | P17183. Positions 2-434. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P17183. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000004267. Mus musculus. [Contig view] |
| GeneID | 13807. |
| KEGG | mmu:13807. |
Organism-specific databases | |
| MGI | MGI:95394. Eno2. |
Phylogenomic databases | |
| HOGENOM | P17183. |
| HOVERGEN | P17183. |
Gene expression databases | |
| ArrayExpress | P17183. |
| CleanEx | MM_ENO2. |
| GermOnline | ENSMUSG00000004267. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000941. Enolase. [Graphical view] |
| PANTHER | PTHR11902. Enolase. 1 hit. |
| Pfam | PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF001400. Enolase. 1 hit. |
| PRINTS | PR00148. ENOLASE. |
| ProDom | PD000902. Enolase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR01060. eno. 1 hit. |
| PROSITE | PS00164. ENOLASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 284588. |
| SOURCE | Search... |
Entry information
| Entry name | ENOG_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P17183 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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