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P17183

- ENOG_MOUSE

UniProt

P17183 - ENOG_MOUSE

Protein

Gamma-enolase

Gene

Eno2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival By similarity.By similarity

    Catalytic activityi

    2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

    Cofactori

    Magnesium. Required for catalysis and for stabilizing the dimer.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei158 – 1581SubstrateBy similarity
    Binding sitei167 – 1671SubstrateBy similarity
    Active sitei210 – 2101Proton donorBy similarity
    Metal bindingi245 – 2451MagnesiumBy similarity
    Metal bindingi293 – 2931MagnesiumBy similarity
    Binding sitei293 – 2931SubstrateBy similarity
    Metal bindingi318 – 3181MagnesiumBy similarity
    Binding sitei318 – 3181SubstrateBy similarity
    Active sitei343 – 3431Proton acceptorBy similarity
    Binding sitei394 – 3941SubstrateBy similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. phosphopyruvate hydratase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP17183.
    UniPathwayiUPA00109; UER00187.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gamma-enolase (EC:4.2.1.11)
    Alternative name(s):
    2-phospho-D-glycerate hydro-lyase
    Enolase 2
    Neural enolase
    Neuron-specific enolase
    Short name:
    NSE
    Gene namesi
    Name:Eno2
    Synonyms:Eno-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:95394. Eno2.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. intracellular Source: MGI
    3. neuronal cell body Source: MGI
    4. perikaryon Source: MGI
    5. phosphopyruvate hydratase complex Source: InterPro
    6. photoreceptor inner segment Source: MGI
    7. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 434434Gamma-enolasePRO_0000134113Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei26 – 261PhosphothreonineBy similarity
    Modified residuei44 – 441PhosphotyrosineBy similarity
    Modified residuei197 – 1971N6-acetyllysine1 Publication
    Modified residuei199 – 1991N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP17183.
    PaxDbiP17183.
    PRIDEiP17183.

    2D gel databases

    UCD-2DPAGEP17183.

    PTM databases

    PhosphoSiteiP17183.

    Expressioni

    Tissue specificityi

    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

    Developmental stagei

    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

    Gene expression databases

    ArrayExpressiP17183.
    BgeeiP17183.
    CleanExiMM_ENO2.
    GenevestigatoriP17183.

    Interactioni

    Subunit structurei

    Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

    Protein-protein interaction databases

    IntActiP17183. 3 interactions.
    MINTiMINT-4094426.

    Structurei

    3D structure databases

    ProteinModelPortaliP17183.
    SMRiP17183. Positions 2-434.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni370 – 3734Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the enolase family.Curated

    Phylogenomic databases

    eggNOGiCOG0148.
    HOGENOMiHOG000072174.
    HOVERGENiHBG000067.
    InParanoidiP17183.
    KOiK01689.
    OMAiHANWQGA.
    PhylomeDBiP17183.
    TreeFamiTF300391.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPiMF_00318. Enolase.
    InterProiIPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view]
    PANTHERiPTHR11902. PTHR11902. 1 hit.
    PfamiPF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001400. Enolase. 1 hit.
    PRINTSiPR00148. ENOLASE.
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01060. eno. 1 hit.
    PROSITEiPS00164. ENOLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17183-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR    50
    DGDKQRYLGK GVLKAVDHIN SRIAPALISS GISVVEQEKL DNLMLELDGT 100
    ENKSKFGANA ILGVSLAVCK AGAAERDLPL YRHIAQLAGN SDLILPVPAF 150
    NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY 200
    GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKM VIGMDVAASE 250
    FYRDGKYDLD FKSPADPSRY ITGDQLGALY QDFVRNYPVV SIEDPFDQDD 300
    WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV 350
    TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR 400
    SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL 434
    Length:434
    Mass (Da):47,297
    Last modified:January 23, 2007 - v2
    Checksum:iA5C7F189E913392E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52380 mRNA. Translation: CAA36606.1.
    AC002397 Genomic DNA. Translation: AAC36002.1.
    BC031739 mRNA. Translation: AAH31739.1.
    CCDSiCCDS20527.1.
    PIRiS10247.
    RefSeqiNP_038537.1. NM_013509.2.
    XP_006505566.1. XM_006505503.1.
    XP_006505567.1. XM_006505504.1.
    UniGeneiMm.3913.

    Genome annotation databases

    EnsembliENSMUST00000004378; ENSMUSP00000004378; ENSMUSG00000004267.
    GeneIDi13807.
    KEGGimmu:13807.
    UCSCiuc009drs.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52380 mRNA. Translation: CAA36606.1 .
    AC002397 Genomic DNA. Translation: AAC36002.1 .
    BC031739 mRNA. Translation: AAH31739.1 .
    CCDSi CCDS20527.1.
    PIRi S10247.
    RefSeqi NP_038537.1. NM_013509.2.
    XP_006505566.1. XM_006505503.1.
    XP_006505567.1. XM_006505504.1.
    UniGenei Mm.3913.

    3D structure databases

    ProteinModelPortali P17183.
    SMRi P17183. Positions 2-434.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P17183. 3 interactions.
    MINTi MINT-4094426.

    PTM databases

    PhosphoSitei P17183.

    2D gel databases

    UCD-2DPAGE P17183.

    Proteomic databases

    MaxQBi P17183.
    PaxDbi P17183.
    PRIDEi P17183.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004378 ; ENSMUSP00000004378 ; ENSMUSG00000004267 .
    GeneIDi 13807.
    KEGGi mmu:13807.
    UCSCi uc009drs.1. mouse.

    Organism-specific databases

    CTDi 2026.
    MGIi MGI:95394. Eno2.

    Phylogenomic databases

    eggNOGi COG0148.
    HOGENOMi HOG000072174.
    HOVERGENi HBG000067.
    InParanoidi P17183.
    KOi K01689.
    OMAi HANWQGA.
    PhylomeDBi P17183.
    TreeFami TF300391.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00187 .
    SABIO-RK P17183.

    Miscellaneous databases

    NextBioi 284588.
    PROi P17183.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P17183.
    Bgeei P17183.
    CleanExi MM_ENO2.
    Genevestigatori P17183.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPi MF_00318. Enolase.
    InterProi IPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view ]
    PANTHERi PTHR11902. PTHR11902. 1 hit.
    Pfami PF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001400. Enolase. 1 hit.
    PRINTSi PR00148. ENOLASE.
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR01060. eno. 1 hit.
    PROSITEi PS00164. ENOLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of cDNAs alpha and gamma enolase mRNAs from mouse brain."
      Kaghad M., Dumont X., Chalon P., Lelias J.M., Lamande N., Lucas M., Lazar M., Caput D.
      Nucleic Acids Res. 18:3638-3638(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6."
      Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., Gibbs R.A.
      Genome Res. 8:29-40(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Retina.
    4. Lubec G., Klug S., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 16-28; 33-50; 73-89; 104-120; 163-179; 184-193; 203-228; 240-262; 270-285; 307-326; 336-358; 373-394 AND 413-429, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain and Hippocampus.
    5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-197 AND LYS-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiENOG_MOUSE
    AccessioniPrimary (citable) accession number: P17183
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3