Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sterol 26-hydroxylase, mitochondrial

Gene

Cyp27a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.By similarity

Catalytic activityi

5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + 6 reduced adrenodoxin + 6 H+ + 3 O2 = (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + 6 oxidized adrenodoxin + 4 H2O.By similarity

Cofactori

hemeBy similarity

Pathwayi: cholecalciferol biosynthesis

This protein is involved in the pathway cholecalciferol biosynthesis, which is part of Hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway cholecalciferol biosynthesis and in Hormone biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi479 – 4791Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

  • cholesterol metabolic process Source: RGD
  • steroid catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14308.
SABIO-RKP17178.
UniPathwayiUPA00955.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 26-hydroxylase, mitochondrial (EC:1.14.15.15By similarity)
Alternative name(s):
5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase
Cytochrome P-450C27/25
Cytochrome P450 27
Sterol 27-hydroxylase
Vitamin D(3) 25-hydroxylase
Gene namesi
Name:Cyp27a1
Synonyms:Cyp27
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727915. Cyp27a1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial envelope Source: RGD
  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232Mitochondrion1 PublicationAdd
BLAST
Chaini33 – 533501Sterol 26-hydroxylase, mitochondrialPRO_0000003621Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei142 – 1421N6-acetyllysineBy similarity
Modified residuei375 – 3751N6-acetyllysineBy similarity
Modified residuei512 – 5121N6-acetyllysineBy similarity
Modified residuei523 – 5231N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP17178.
PRIDEiP17178.

Expressioni

Tissue specificityi

Expressed in liver, hepatoma, kidney, ovary and epithelial cells of blood vessels.

Gene expression databases

GenevisibleiP17178. RN.

Interactioni

Protein-protein interaction databases

BioGridi256990. 1 interaction.
MINTiMINT-4578798.
STRINGi10116.ENSRNOP00000023152.

Structurei

3D structure databases

ProteinModelPortaliP17178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni386 – 40015Sterol-bindingSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0159. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000253961.
HOVERGENiHBG106909.
InParanoidiP17178.
KOiK00488.
OMAiCPQGARA.
OrthoDBiEOG7ZGX4B.
PhylomeDBiP17178.
TreeFamiTF105094.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17178-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVLSRMRLR WALLDTRVMG HGLCPQGARA KAAIPAALRD HESTEGPGTG
60 70 80 90 100
QDRPRLRSLA ELPGPGTLRF LFQLFLRGYV LHLHELQALN KAKYGPMWTT
110 120 130 140 150
TFGTRTNVNL ASAPLLEQVM RQEGKYPIRD SMEQWKEHRD HKGLSYGIFI
160 170 180 190 200
TQGQQWYHLR HSLNQRMLKP AEAALYTDAL NEVISDFIAR LDQVRTESAS
210 220 230 240 250
GDQVPDVAHL LYHLALEAIC YILFEKRVGC LEPSIPEDTA TFIRSVGLMF
260 270 280 290 300
KNSVYVTFLP KWSRPLLPFW KRYMNNWDNI FSFGEKMIHQ KVQEIEAQLQ
310 320 330 340 350
AAGPDGVQVS GYLHFLLTKE LLSPQETVGT FPELILAGVD TTSNTLTWAL
360 370 380 390 400
YHLSKNPEIQ EALHKEVTGV VPFGKVPQNK DFAHMPLLKA VIKETLRLYP
410 420 430 440 450
VVPTNSRIIT EKETEINGFL FPKNTQFVLC HYVVSRDPSV FPEPESFQPH
460 470 480 490 500
RWLRKREDDN SGIQHPFGSV PFGYGVRSCL GRRIAELEMQ LLLSRLIQKY
510 520 530
EVVLSPGMGE VKSVSRIVLV PSKKVSLRFL QRQ
Length:533
Mass (Da):60,733
Last modified:August 1, 1990 - v1
Checksum:iDC9153BEB6471D63
GO

Sequence cautioni

The sequence AAA86314.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 969Missing in AAA86314 (PubMed:7577965).Curated
Sequence conflicti167 – 1682ML → IV in AAA41786 (PubMed:1733943).Curated
Sequence conflicti167 – 1682ML → IV in AAA86314 (PubMed:7577965).Curated
Sequence conflicti209 – 2091H → N in AAA41786 (PubMed:1733943).Curated
Sequence conflicti209 – 2091H → N in AAA86314 (PubMed:7577965).Curated
Sequence conflicti358 – 3581E → H in AAA86314 (PubMed:7577965).Curated
Sequence conflicti364 – 3641Missing in AAA86314 (PubMed:7577965).Curated
Sequence conflicti393 – 3931K → P in AAA86314 (PubMed:7577965).Curated
Sequence conflicti431 – 4311H → T in AAB02287 (PubMed:2175615).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07534 Genomic DNA. Translation: CAA68822.1.
M38566 mRNA. Translation: AAB02287.1.
M73231 mRNA. Translation: AAA41786.1.
U17375
, U17363, U17369, U17370, U17371, U17372, U17373, U17374, U17376 Genomic DNA. Translation: AAA86314.1. Different initiation.
BC061848 mRNA. Translation: AAH61848.1.
PIRiB42324.
S09198. O4RTV3.
RefSeqiNP_849178.2. NM_178847.3.
UniGeneiRn.94956.

Genome annotation databases

GeneIDi301517.
KEGGirno:301517.
UCSCiRGD:727915. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07534 Genomic DNA. Translation: CAA68822.1.
M38566 mRNA. Translation: AAB02287.1.
M73231 mRNA. Translation: AAA41786.1.
U17375
, U17363, U17369, U17370, U17371, U17372, U17373, U17374, U17376 Genomic DNA. Translation: AAA86314.1. Different initiation.
BC061848 mRNA. Translation: AAH61848.1.
PIRiB42324.
S09198. O4RTV3.
RefSeqiNP_849178.2. NM_178847.3.
UniGeneiRn.94956.

3D structure databases

ProteinModelPortaliP17178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi256990. 1 interaction.
MINTiMINT-4578798.
STRINGi10116.ENSRNOP00000023152.

Proteomic databases

PaxDbiP17178.
PRIDEiP17178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi301517.
KEGGirno:301517.
UCSCiRGD:727915. rat.

Organism-specific databases

CTDi1593.
RGDi727915. Cyp27a1.

Phylogenomic databases

eggNOGiKOG0159. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000253961.
HOVERGENiHBG106909.
InParanoidiP17178.
KOiK00488.
OMAiCPQGARA.
OrthoDBiEOG7ZGX4B.
PhylomeDBiP17178.
TreeFamiTF105094.

Enzyme and pathway databases

UniPathwayiUPA00955.
BioCyciMetaCyc:MONOMER-14308.
SABIO-RKP17178.

Miscellaneous databases

PROiP17178.

Gene expression databases

GenevisibleiP17178. RN.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of cDNA for vitamin D3 25-hydroxylase from rat liver mitochondria."
    Usui E., Noshiro M., Okuda K.
    FEBS Lett. 262:135-138(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 33-37.
    Strain: Wistar.
    Tissue: Liver.
  2. "A cDNA encoding a rat mitochondrial cytochrome P450 catalyzing both the 26-hydroxylation of cholesterol and 25-hydroxylation of vitamin D3: gonadotropic regulation of the cognate mRNA in ovaries."
    Su P., Rennert H., Shayiq R.M., Yamamoto R., Zheng Y.-M., Addya S., Strauss J.F. III, Avadhani N.G.
    DNA Cell Biol. 9:657-667(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Sequence complementarity between the 5'-terminal regions of mRNAs for rat mitochondrial cytochrome P-450c27/25 and a growth hormone-inducible serine protease inhibitor. A possible gene overlap."
    Shayiq R.M., Avadhani N.G.
    J. Biol. Chem. 267:2421-2428(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  4. "Localization of a transcription promoter within the second exon of the cytochrome P-450c27/25 gene for the expression of the major species of two-kilobase mRNA."
    Mullick J., Addya S., Sucharov C., Avadhani N.G.
    Biochemistry 34:13729-13742(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.

Entry informationi

Entry nameiCP27A_RAT
AccessioniPrimary (citable) accession number: P17178
Secondary accession number(s): Q64615, Q64639
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 6, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.