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P17172 (VG27_BPT4) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Baseplate structural protein Gp27
Alternative name(s):
Hub protein 27
Gene names
Name:27
OrganismEnterobacteria phage T4 (Bacteriophage T4) [Reference proteome]
Taxonomic identifier10665 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Structural component of the baseplate. Ref.4

Subunit structure

Homotrimer. Interacts with gp5; this interaction forms the tail lysozyme complex. The T4 tail lysozyme complex is made up of three copies of the proteolytically processed gp5 and three copies of gp27. Ref.4

Subcellular location

Virion Potential.

Ontologies

Keywords
   Cellular componentVirion
   Developmental stageLate protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentvirion

Inferred from direct assay PubMed 18524932. Source: CACAO

   Molecular_functionprotein binding

Inferred from physical interaction Ref.5Ref.7. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

5P160092EBI-1032762,EBI-1032754

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391Baseplate structural protein Gp27
PRO_0000165015

Experimental info

Sequence conflict1681N → T in CAA36481. Ref.1
Sequence conflict2621S → E Ref.1
Sequence conflict3081A → L in CAA36481. Ref.1

Secondary structure

.............................................................. 391
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P17172 [UniParc].

Last modified December 19, 2001. Version 4.
Checksum: 0DABEFE1D890B1FA

FASTA39144,389
        10         20         30         40         50         60 
MSMLQRPGYP NLSVKLFDSY DAWSNNRFVE LAATITTLTM RDSLYGRNEG MLQFYDSKNI 

        70         80         90        100        110        120 
HTKMDGNEII QISVANANDI NNVKTRIYGC KHFSVSVDSK GDNIIAIELG TIHSIENLKF 

       130        140        150        160        170        180 
GRPFFPDAGE SIKEMLGVIY QDRTLLTPAI NAINAYVPDI PWTSTFENYL SYVREVALAV 

       190        200        210        220        230        240 
GSDKFVFVWQ DIMGVNMMDY DMMINQEPYP MIVGEPSLIG QFIQELKYPL AYDFVWLTKS 

       250        260        270        280        290        300 
NPHKRDPMKN ATIYAHSFLD SSIPMITTGK GENSIVVSRS GAYSEMTYRN GYEEAIRLQT 

       310        320        330        340        350        360 
MAQYDGYAKC STIGNFNLTP GVKIIFNDSK NQFKTEFYVD EVIHELSNNN SVTHLYMFTN 

       370        380        390 
ATKLETIDPV KVKNEFKSDT TTEESSSSNK Q 

« Hide

References

« Hide 'large scale' references
[1]"Bacteriophage T4 gene 27."
Bova R., Cascino A., Cipollaro M., Grau O., Micheli M.R., Santoro M., Storlazzi A., Scarlato V., Gargano S.
Nucleic Acids Res. 18:3046-3046(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: D.
[2]"Bacteriophage T4 genome."
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.
Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Bacteriophage T4 late gene expression: overlapping promoters direct divergent transcription of the base plate gene cluster."
Scarlato V., Storlazzi A., Gargano S., Cascino A.
Virology 171:475-483(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
Strain: D.
[4]"Homotrimeric, beta-stranded viral adhesins and tail proteins."
Weigele P.R., Scanlon E., King J.
J. Bacteriol. 185:4022-4030(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH GP5.
[5]"Structure of the cell-puncturing device of bacteriophage T4."
Kanamaru S., Leiman P.G., Kostyuchenko V.A., Chipman P.R., Mesyanzhinov V.V., Arisaka F., Rossmann M.G.
Nature 415:553-557(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
[6]"Three-dimensional structure of bacteriophage T4 baseplate."
Kostyuchenko V.A., Leiman P.G., Chipman P.R., Kanamaru S., van Raaij M.J., Arisaka F., Mesyanzhinov V.V., Rossmann M.G.
Nat. Struct. Biol. 10:688-693(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS).
[7]"Control of bacteriophage T4 tail lysozyme activity during the infection process."
Kanamaru S., Ishiwata Y., Suzuki T., Rossmann M.G., Arisaka F.
J. Mol. Biol. 346:1013-1020(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52236 Genomic DNA. Translation: CAA36481.1.
AF158101 Genomic DNA. Translation: AAD42435.2.
J04354 Genomic DNA. Translation: AAA88466.1.
PIRGZBPT4. JU0285.
RefSeqNP_049803.1. NC_000866.4.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K28X-ray2.90D1-391[»]
1PDJelectron microscopy12.00D/E/F1-391[»]
1WTHX-ray2.80D1-391[»]
2Z6BX-ray3.11D1-391[»]
ProteinModelPortalP17172.
SMRP17172. Positions 4-377.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP17172. 1 interaction.
MINTMINT-231925.

Protein family/group databases

TCDB1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1258643.

Family and domain databases

InterProIPR015180. Phage_T4_Gp27_C.
IPR015181. Phage_T4_Gp27_N.
[Graphical view]
PfamPF09097. Phage-tail_1. 1 hit.
PF09096. Phage-tail_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP17172.

Entry information

Entry nameVG27_BPT4
AccessionPrimary (citable) accession number: P17172
Secondary accession number(s): Q9T0T8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: December 19, 2001
Last modified: June 11, 2014
This is version 80 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references