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P17172

- BP27_BPT4

UniProt

P17172 - BP27_BPT4

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Protein

Base plate structural protein gp27

Gene

27

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Structural component of the baseplate hub. The baseplate is a multicomponent protein complex which controls phage attachment to the host. During infection, the baseplate undergoes a conformational change from a dome-shaped to a star-shaped structure.1 Publication

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Base plate structural protein gp27
Alternative name(s):
Gene product 27
Short name:
gp27
Hub protein 27
Gene namesi
Name:27
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087: Genome

Subcellular locationi

Virion 1 Publication

GO - Cellular componenti

  1. virion Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Viral baseplate protein, Viral tail protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Base plate structural protein gp27PRO_0000165015Add
BLAST

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer. Interacts with gp5; this interaction forms the tail lysozyme complex. The T4 tail lysozyme complex is made up of three copies of the proteolytically processed gp5 and three copies of gp27. The baseplate hub of bacteriophage T4 consists of six gene products namely gp5, gp26, gp27, gp28, gp48 and gp54.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
5P160092EBI-1032762,EBI-1032754

Protein-protein interaction databases

IntActiP17172. 1 interaction.
MINTiMINT-231925.

Structurei

Secondary structure

1
391
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 208
Helixi21 – 244
Beta strandi32 – 4312
Beta strandi48 – 547
Helixi60 – 623
Beta strandi69 – 746
Beta strandi76 – 783
Beta strandi84 – 9310
Beta strandi99 – 1013
Beta strandi105 – 1106
Helixi112 – 1154
Helixi128 – 13912
Turni140 – 1467
Beta strandi149 – 1513
Helixi166 – 17611
Turni180 – 1834
Beta strandi184 – 1907
Beta strandi195 – 1995
Helixi200 – 2045
Beta strandi209 – 2135
Beta strandi230 – 23910
Helixi242 – 2443
Helixi247 – 2504
Beta strandi251 – 2566
Beta strandi258 – 2614
Beta strandi265 – 2684
Beta strandi273 – 2786
Helixi281 – 2855
Beta strandi286 – 2916
Helixi292 – 30110
Beta strandi306 – 3138
Beta strandi323 – 3275
Beta strandi335 – 34612
Beta strandi351 – 3599

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K28X-ray2.90D1-391[»]
1PDJelectron microscopy12.00D/E/F1-391[»]
1WTHX-ray2.80D1-391[»]
2Z6BX-ray3.11D1-391[»]
ProteinModelPortaliP17172.
SMRiP17172. Positions 4-377.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17172.

Family & Domainsi

Family and domain databases

InterProiIPR015180. Phage_T4_Gp27_C.
IPR015181. Phage_T4_Gp27_N.
[Graphical view]
PfamiPF09097. Phage-tail_1. 1 hit.
PF09096. Phage-tail_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17172-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSMLQRPGYP NLSVKLFDSY DAWSNNRFVE LAATITTLTM RDSLYGRNEG
60 70 80 90 100
MLQFYDSKNI HTKMDGNEII QISVANANDI NNVKTRIYGC KHFSVSVDSK
110 120 130 140 150
GDNIIAIELG TIHSIENLKF GRPFFPDAGE SIKEMLGVIY QDRTLLTPAI
160 170 180 190 200
NAINAYVPDI PWTSTFENYL SYVREVALAV GSDKFVFVWQ DIMGVNMMDY
210 220 230 240 250
DMMINQEPYP MIVGEPSLIG QFIQELKYPL AYDFVWLTKS NPHKRDPMKN
260 270 280 290 300
ATIYAHSFLD SSIPMITTGK GENSIVVSRS GAYSEMTYRN GYEEAIRLQT
310 320 330 340 350
MAQYDGYAKC STIGNFNLTP GVKIIFNDSK NQFKTEFYVD EVIHELSNNN
360 370 380 390
SVTHLYMFTN ATKLETIDPV KVKNEFKSDT TTEESSSSNK Q
Length:391
Mass (Da):44,389
Last modified:December 19, 2001 - v4
Checksum:i0DABEFE1D890B1FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti168 – 1681N → T in CAA36481. (PubMed:2349100)Curated
Sequence conflicti262 – 2621S → E(PubMed:2349100)Curated
Sequence conflicti308 – 3081A → L in CAA36481. (PubMed:2349100)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52236 Genomic DNA. Translation: CAA36481.1.
AF158101 Genomic DNA. Translation: AAD42435.2.
J04354 Genomic DNA. Translation: AAA88466.1.
PIRiJU0285. GZBPT4.
RefSeqiNP_049803.1. NC_000866.4.

Genome annotation databases

GeneIDi1258643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52236 Genomic DNA. Translation: CAA36481.1 .
AF158101 Genomic DNA. Translation: AAD42435.2 .
J04354 Genomic DNA. Translation: AAA88466.1 .
PIRi JU0285. GZBPT4.
RefSeqi NP_049803.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K28 X-ray 2.90 D 1-391 [» ]
1PDJ electron microscopy 12.00 D/E/F 1-391 [» ]
1WTH X-ray 2.80 D 1-391 [» ]
2Z6B X-ray 3.11 D 1-391 [» ]
ProteinModelPortali P17172.
SMRi P17172. Positions 4-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P17172. 1 interaction.
MINTi MINT-231925.

Protein family/group databases

TCDBi 1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1258643.

Miscellaneous databases

EvolutionaryTracei P17172.

Family and domain databases

InterProi IPR015180. Phage_T4_Gp27_C.
IPR015181. Phage_T4_Gp27_N.
[Graphical view ]
Pfami PF09097. Phage-tail_1. 1 hit.
PF09096. Phage-tail_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Bacteriophage T4 late gene expression: overlapping promoters direct divergent transcription of the base plate gene cluster."
    Scarlato V., Storlazzi A., Gargano S., Cascino A.
    Virology 171:475-483(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
    Strain: D.
  4. "Homotrimeric, beta-stranded viral adhesins and tail proteins."
    Weigele P.R., Scanlon E., King J.
    J. Bacteriol. 185:4022-4030(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GP5.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
  6. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS).
  7. "Control of bacteriophage T4 tail lysozyme activity during the infection process."
    Kanamaru S., Ishiwata Y., Suzuki T., Rossmann M.G., Arisaka F.
    J. Mol. Biol. 346:1013-1020(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

Entry informationi

Entry nameiBP27_BPT4
AccessioniPrimary (citable) accession number: P17172
Secondary accession number(s): Q9T0T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: December 19, 2001
Last modified: October 29, 2014
This is version 83 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3