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P17172

- BP27_BPT4

UniProt

P17172 - BP27_BPT4

Protein

Base plate structural protein gp27

Gene

27

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 4 (19 Dec 2001)
      Previous versions | rss
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    Functioni

    Structural component of the baseplate hub. The baseplate is a multicomponent protein complex which controls phage attachment to the host. During infection, the baseplate undergoes a conformational change from a dome-shaped to a star-shaped structure.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    Protein family/group databases

    TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Base plate structural protein gp27
    Alternative name(s):
    Gene product 27
    Short name:
    gp27
    Hub protein 27
    Gene namesi
    Name:27
    OrganismiEnterobacteria phage T4 (Bacteriophage T4)
    Taxonomic identifieri10665 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000009087: Genome

    Subcellular locationi

    Virion 1 Publication

    GO - Cellular componenti

    1. virion Source: CACAO

    Keywords - Cellular componenti

    Viral baseplate protein, Viral tail protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391Base plate structural protein gp27PRO_0000165015Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with gp5; this interaction forms the tail lysozyme complex. The T4 tail lysozyme complex is made up of three copies of the proteolytically processed gp5 and three copies of gp27. The baseplate hub of bacteriophage T4 consists of six gene products namely gp5, gp26, gp27, gp28, gp48 and gp54.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    5P160092EBI-1032762,EBI-1032754

    Protein-protein interaction databases

    IntActiP17172. 1 interaction.
    MINTiMINT-231925.

    Structurei

    Secondary structure

    1
    391
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi13 – 208
    Helixi21 – 244
    Beta strandi32 – 4312
    Beta strandi48 – 547
    Helixi60 – 623
    Beta strandi69 – 746
    Beta strandi76 – 783
    Beta strandi84 – 9310
    Beta strandi99 – 1013
    Beta strandi105 – 1106
    Helixi112 – 1154
    Helixi128 – 13912
    Turni140 – 1467
    Beta strandi149 – 1513
    Helixi166 – 17611
    Turni180 – 1834
    Beta strandi184 – 1907
    Beta strandi195 – 1995
    Helixi200 – 2045
    Beta strandi209 – 2135
    Beta strandi230 – 23910
    Helixi242 – 2443
    Helixi247 – 2504
    Beta strandi251 – 2566
    Beta strandi258 – 2614
    Beta strandi265 – 2684
    Beta strandi273 – 2786
    Helixi281 – 2855
    Beta strandi286 – 2916
    Helixi292 – 30110
    Beta strandi306 – 3138
    Beta strandi323 – 3275
    Beta strandi335 – 34612
    Beta strandi351 – 3599

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K28X-ray2.90D1-391[»]
    1PDJelectron microscopy12.00D/E/F1-391[»]
    1WTHX-ray2.80D1-391[»]
    2Z6BX-ray3.11D1-391[»]
    ProteinModelPortaliP17172.
    SMRiP17172. Positions 4-377.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17172.

    Family & Domainsi

    Family and domain databases

    InterProiIPR015180. Phage_T4_Gp27_C.
    IPR015181. Phage_T4_Gp27_N.
    [Graphical view]
    PfamiPF09097. Phage-tail_1. 1 hit.
    PF09096. Phage-tail_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17172-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMLQRPGYP NLSVKLFDSY DAWSNNRFVE LAATITTLTM RDSLYGRNEG    50
    MLQFYDSKNI HTKMDGNEII QISVANANDI NNVKTRIYGC KHFSVSVDSK 100
    GDNIIAIELG TIHSIENLKF GRPFFPDAGE SIKEMLGVIY QDRTLLTPAI 150
    NAINAYVPDI PWTSTFENYL SYVREVALAV GSDKFVFVWQ DIMGVNMMDY 200
    DMMINQEPYP MIVGEPSLIG QFIQELKYPL AYDFVWLTKS NPHKRDPMKN 250
    ATIYAHSFLD SSIPMITTGK GENSIVVSRS GAYSEMTYRN GYEEAIRLQT 300
    MAQYDGYAKC STIGNFNLTP GVKIIFNDSK NQFKTEFYVD EVIHELSNNN 350
    SVTHLYMFTN ATKLETIDPV KVKNEFKSDT TTEESSSSNK Q 391
    Length:391
    Mass (Da):44,389
    Last modified:December 19, 2001 - v4
    Checksum:i0DABEFE1D890B1FA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti168 – 1681N → T in CAA36481. (PubMed:2349100)Curated
    Sequence conflicti262 – 2621S → E(PubMed:2349100)Curated
    Sequence conflicti308 – 3081A → L in CAA36481. (PubMed:2349100)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52236 Genomic DNA. Translation: CAA36481.1.
    AF158101 Genomic DNA. Translation: AAD42435.2.
    J04354 Genomic DNA. Translation: AAA88466.1.
    PIRiJU0285. GZBPT4.
    RefSeqiNP_049803.1. NC_000866.4.

    Genome annotation databases

    GeneIDi1258643.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52236 Genomic DNA. Translation: CAA36481.1 .
    AF158101 Genomic DNA. Translation: AAD42435.2 .
    J04354 Genomic DNA. Translation: AAA88466.1 .
    PIRi JU0285. GZBPT4.
    RefSeqi NP_049803.1. NC_000866.4.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K28 X-ray 2.90 D 1-391 [» ]
    1PDJ electron microscopy 12.00 D/E/F 1-391 [» ]
    1WTH X-ray 2.80 D 1-391 [» ]
    2Z6B X-ray 3.11 D 1-391 [» ]
    ProteinModelPortali P17172.
    SMRi P17172. Positions 4-377.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P17172. 1 interaction.
    MINTi MINT-231925.

    Protein family/group databases

    TCDBi 1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1258643.

    Miscellaneous databases

    EvolutionaryTracei P17172.

    Family and domain databases

    InterProi IPR015180. Phage_T4_Gp27_C.
    IPR015181. Phage_T4_Gp27_N.
    [Graphical view ]
    Pfami PF09097. Phage-tail_1. 1 hit.
    PF09096. Phage-tail_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: D.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Bacteriophage T4 late gene expression: overlapping promoters direct divergent transcription of the base plate gene cluster."
      Scarlato V., Storlazzi A., Gargano S., Cascino A.
      Virology 171:475-483(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
      Strain: D.
    4. "Homotrimeric, beta-stranded viral adhesins and tail proteins."
      Weigele P.R., Scanlon E., King J.
      J. Bacteriol. 185:4022-4030(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GP5.
    5. Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
    6. Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS).
    7. "Control of bacteriophage T4 tail lysozyme activity during the infection process."
      Kanamaru S., Ishiwata Y., Suzuki T., Rossmann M.G., Arisaka F.
      J. Mol. Biol. 346:1013-1020(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).

    Entry informationi

    Entry nameiBP27_BPT4
    AccessioniPrimary (citable) accession number: P17172
    Secondary accession number(s): Q9T0T8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: December 19, 2001
    Last modified: October 1, 2014
    This is version 82 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3