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Protein

Baseplate central spike complex protein gp27

Gene

27

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Baseplate central spike complex-associated protein involved in the tail assembly.1 Publication2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Viral tail assembly, Virus exit from host cell

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate central spike complex protein gp271 Publication
Alternative name(s):
Gene product 27
Short name:
gp27
Hub protein 27
Gene namesi
Name:27
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000009087 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • virion Source: CACAO
  • virus tail, baseplate Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Viral baseplate protein, Viral tail protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001650151 – 391Baseplate central spike complex protein gp27Add BLAST391

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.Curated

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer (PubMed:12837775, PubMed:19896486, PubMed:11823865). Heteromultimer with gp5 trimer and gp5.4 monomer; this interaction forms the tail lysozyme complex that creates an extension of the tail tube (PubMed:12837775, PubMed:27193680). The T4 tail lysozyme complex is made up of three copies of the proteolytically processed gp5 and three copies of gp27.Part of the baseplate macromolecular complex which consists of gp5, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and gp54 (proximal region of the tail tube) (PubMed:27193680).1 Publication4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
5P160092EBI-1032762,EBI-1032754

Protein-protein interaction databases

IntActiP17172. 1 interactor.
MINTiMINT-231925.

Structurei

Secondary structure

1391
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi13 – 20Combined sources8
Helixi21 – 24Combined sources4
Beta strandi32 – 43Combined sources12
Beta strandi48 – 54Combined sources7
Helixi60 – 62Combined sources3
Beta strandi69 – 74Combined sources6
Beta strandi76 – 78Combined sources3
Beta strandi84 – 93Combined sources10
Beta strandi99 – 101Combined sources3
Beta strandi105 – 110Combined sources6
Helixi112 – 115Combined sources4
Helixi128 – 139Combined sources12
Turni140 – 146Combined sources7
Beta strandi149 – 151Combined sources3
Helixi166 – 176Combined sources11
Turni180 – 183Combined sources4
Beta strandi184 – 190Combined sources7
Beta strandi195 – 199Combined sources5
Helixi200 – 204Combined sources5
Beta strandi209 – 213Combined sources5
Beta strandi230 – 239Combined sources10
Helixi242 – 244Combined sources3
Helixi247 – 250Combined sources4
Beta strandi251 – 256Combined sources6
Beta strandi258 – 261Combined sources4
Beta strandi265 – 268Combined sources4
Beta strandi273 – 278Combined sources6
Helixi281 – 285Combined sources5
Beta strandi286 – 291Combined sources6
Helixi292 – 301Combined sources10
Beta strandi306 – 313Combined sources8
Beta strandi323 – 327Combined sources5
Beta strandi335 – 346Combined sources12
Beta strandi351 – 359Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K28X-ray2.90D1-391[»]
1PDJelectron microscopy12.00D/E/F1-391[»]
1WTHX-ray2.80D1-391[»]
2Z6BX-ray3.11D1-391[»]
5IV5electron microscopy4.11YD/YE/YF1-391[»]
ProteinModelPortaliP17172.
SMRiP17172.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17172.

Family & Domainsi

Family and domain databases

InterProiIPR015180. Phage_T4_Gp27_C.
IPR015181. Phage_T4_Gp27_N.
[Graphical view]
PfamiPF09097. Phage-tail_1. 1 hit.
PF09096. Phage-tail_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMLQRPGYP NLSVKLFDSY DAWSNNRFVE LAATITTLTM RDSLYGRNEG
60 70 80 90 100
MLQFYDSKNI HTKMDGNEII QISVANANDI NNVKTRIYGC KHFSVSVDSK
110 120 130 140 150
GDNIIAIELG TIHSIENLKF GRPFFPDAGE SIKEMLGVIY QDRTLLTPAI
160 170 180 190 200
NAINAYVPDI PWTSTFENYL SYVREVALAV GSDKFVFVWQ DIMGVNMMDY
210 220 230 240 250
DMMINQEPYP MIVGEPSLIG QFIQELKYPL AYDFVWLTKS NPHKRDPMKN
260 270 280 290 300
ATIYAHSFLD SSIPMITTGK GENSIVVSRS GAYSEMTYRN GYEEAIRLQT
310 320 330 340 350
MAQYDGYAKC STIGNFNLTP GVKIIFNDSK NQFKTEFYVD EVIHELSNNN
360 370 380 390
SVTHLYMFTN ATKLETIDPV KVKNEFKSDT TTEESSSSNK Q
Length:391
Mass (Da):44,389
Last modified:December 19, 2001 - v4
Checksum:i0DABEFE1D890B1FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti168N → T in CAA36481 (PubMed:2349100).Curated1
Sequence conflicti262S → E (PubMed:2349100).Curated1
Sequence conflicti308A → L in CAA36481 (PubMed:2349100).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52236 Genomic DNA. Translation: CAA36481.1.
AF158101 Genomic DNA. Translation: AAD42435.2.
J04354 Genomic DNA. Translation: AAA88466.1.
PIRiJU0285. GZBPT4.
RefSeqiNP_049803.1. NC_000866.4.

Genome annotation databases

GeneIDi1258643.
KEGGivg:1258643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52236 Genomic DNA. Translation: CAA36481.1.
AF158101 Genomic DNA. Translation: AAD42435.2.
J04354 Genomic DNA. Translation: AAA88466.1.
PIRiJU0285. GZBPT4.
RefSeqiNP_049803.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K28X-ray2.90D1-391[»]
1PDJelectron microscopy12.00D/E/F1-391[»]
1WTHX-ray2.80D1-391[»]
2Z6BX-ray3.11D1-391[»]
5IV5electron microscopy4.11YD/YE/YF1-391[»]
ProteinModelPortaliP17172.
SMRiP17172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP17172. 1 interactor.
MINTiMINT-231925.

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258643.
KEGGivg:1258643.

Miscellaneous databases

EvolutionaryTraceiP17172.

Family and domain databases

InterProiIPR015180. Phage_T4_Gp27_C.
IPR015181. Phage_T4_Gp27_N.
[Graphical view]
PfamiPF09097. Phage-tail_1. 1 hit.
PF09096. Phage-tail_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBP27_BPT4
AccessioniPrimary (citable) accession number: P17172
Secondary accession number(s): Q9T0T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: December 19, 2001
Last modified: November 2, 2016
This is version 95 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.