P17162 (PTSN_KLEOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrogen regulatory protein

Alternative name(s):
Enzyme IIA-NTR

Including the following 1 domains:

1. Phosphotransferase enzyme IIA component
   EC=2.7.1.-
   Alternative name(s):
   PTS system EIIA component

Gene names

Name:

ptsN

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Seems to have a role in regulating nitrogen assimilation By similarity.
Subcellular location	Cytoplasm.
Domain	The PTS EIIA type-2 domain may serve a regulatory function, through its phosphorylation activity.
Sequence similarities	Contains 1 PTS EIIA type-2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	Nitrogen regulatory protein		PRO_0000186695
Regions
	Domain	12 – 156	145	PTS EIIA type-2
Sites
	Active site	73	1	Tele-phosphohistidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P17162 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 990737929D387B40	FASTA	162	17,720
	10 20 30 40 50 60 MINNDSALQL SNVLNQECTR SQVHCQSKKR ALEIISELAA KQLGLSSQIV FEAILTREKM 70 80 90 100 110 120 GSTGIGNGIA IPHGKLEEDT LRAVGVFVQL ETPIAFDAID NQPVDLLFAL LVPADQTKTH 130 140 150 160 LHTLSLVAKR LADKTICRRL RAAQSDEELY EIITEAGSNN EA

References

[1]

"Mutations in genes downstream of the rpoN gene (encoding sigma 54) of Klebsiella pneumoniae affect expression from sigma 54-dependent promoters." Merrick M.J., Coppard J.R. Mol. Microbiol. 3:1765-1775(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M5a1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16335 Genomic DNA. Translation: CAA34392.1.
PIR	S07661.
3D structure databases
ProteinModelPortal	P17162.
SMR	P17162. Positions 1-155.
ModBase	Search...
Proteomic databases
PRIDE	P17162.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.930.10. 1 hit.
InterPro	IPR016152. PTrfase/Anion_transptr. IPR002178. PTS_EIIA_2. IPR006320. PTS_Nitro_regul. [Graphical view]
Pfam	PF00359. PTS_EIIA_2. 1 hit. [Graphical view]
SUPFAM	SSF55804. PTrfase/Anion_transptr. 1 hit.
TIGRFAMs	TIGR01419. nitro_reg_IIA. 1 hit.
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTSN_KLEOX

Accession

Primary (citable) accession number: P17162

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program