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P17156

- HSP72_MOUSE

UniProt

P17156 - HSP72_MOUSE

Protein

Heat shock-related 70 kDa protein 2

Gene

Hspa2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei72 – 721ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 164ATPBy similarity
    Nucleotide bindingi205 – 2073ATPBy similarity
    Nucleotide bindingi271 – 2788ATPBy similarity
    Nucleotide bindingi342 – 3454ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glycolipid binding Source: MGI
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. male meiosis Source: MGI
    2. male meiosis I Source: MGI
    3. positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle Source: MGI
    4. positive regulation of protein phosphorylation Source: MGI
    5. response to stress Source: UniProtKB-KW
    6. spermatid development Source: MGI
    7. synaptonemal complex disassembly Source: MGI

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198626. Meiotic synapsis.
    REACT_75800. Meiotic Synapsis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat shock-related 70 kDa protein 2
    Short name:
    Heat shock protein 70.2
    Gene namesi
    Name:Hspa2
    Synonyms:Hcp70.2, Hsp70-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:96243. Hspa2.

    Subcellular locationi

    GO - Cellular componenti

    1. CatSper complex Source: UniProtKB
    2. cell surface Source: MGI
    3. male germ cell nucleus Source: MGI
    4. mitochondrion Source: MGI
    5. nucleoplasm Source: Reactome
    6. synaptonemal complex Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 633633Heat shock-related 70 kDa protein 2PRO_0000078259Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei403 – 4031PhosphoserineBy similarity
    Modified residuei408 – 4081PhosphothreonineBy similarity
    Modified residuei414 – 4141PhosphothreonineBy similarity
    Modified residuei564 – 5641N6,N6,N6-trimethyllysine; by METTL21A; in vitroBy similarity

    Keywords - PTMi

    Methylation, Phosphoprotein

    Proteomic databases

    MaxQBiP17156.
    PaxDbiP17156.
    PRIDEiP17156.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00331546.
    P17156.
    UCD-2DPAGEP17156.

    PTM databases

    PhosphoSiteiP17156.

    Expressioni

    Tissue specificityi

    Expressed within the prinicipal peice and midpiece of sperm tail (at protein level).1 Publication

    Developmental stagei

    Specifically expressed in prophage stage of meiosis.

    Gene expression databases

    BgeeiP17156.
    CleanExiMM_HSPA2.
    GenevestigatoriP17156.

    Interactioni

    Subunit structurei

    Interacts with FKBP6 By similarity. Interacts with ZNF541. Component of the CatSper complex. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2.By similarity3 Publications

    Protein-protein interaction databases

    BioGridi200453. 14 interactions.
    DIPiDIP-42071N.
    IntActiP17156. 5 interactions.
    MINTiMINT-1207820.
    STRINGi10090.ENSMUSP00000079306.

    Structurei

    3D structure databases

    ProteinModelPortaliP17156.
    SMRiP17156. Positions 3-613.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the heat shock protein 70 family.Curated

    Phylogenomic databases

    eggNOGiCOG0443.
    GeneTreeiENSGT00750000117237.
    HOGENOMiHOG000228135.
    HOVERGENiHBG051845.
    InParanoidiQ99KD7.
    KOiK03283.
    OMAiEAYLGCK.
    OrthoDBiEOG7PCJGF.
    TreeFamiTF105042.

    Family and domain databases

    Gene3Di1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProiIPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 1 hit.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    SUPFAMiSSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEiPS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17156-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSARGPAIGI DLGTTYSCVG VFQHGKVEII ANDQGNRTTP SYVAFTDTER    50
    LIGDAAKNQV AMNPTNTIFD AKRLIGRKFE DATVQSDMKH WPFRVVSEGG 100
    KPKVQVEYKG EMKTFFPEEI SSMVLTKMKE IAEAYLGGKV QSAVITVPAY 150
    FNDSQRQATK DAGTITGLNV LRIINEPTAA AIAYGLDKKG CAGGEKNVLI 200
    FDLGGGTFDV SILTIEDGIF EVKSTAGDTH LGGEDFDNRM VSHLAEEFKR 250
    KHKKDIGPNK RAVRRLRTAC ERAKRTLSSS TQASIEIDSL YEGVDFYTSI 300
    TRARFEELNA DLFRGTLEPV EKALRDAKLD KGQIQEIVLV GGSTRIPKIQ 350
    KLLQDFFNGK ELNKSINPDE AVAYGAAVQA AILIGDKSEN VQDLLLLDVT 400
    PLSLGIETAG GVMTPLIKRN TTIPTKQTQT FTTYSDNQSS VLVQVYEGER 450
    AMTKDNNLLG KFDLTGIPPA PRGVPQIEVT FDIDANGILN VTAADKSTGK 500
    ENKITITNDK GRLSKDDIDR MVQEAERYKS EDEANRDRVA AKNAVESYTY 550
    NIKQTVEDEK LRGKISEQDK NKILDKCQEV INWLDRNQMA EKDEYEHKQK 600
    ELERVCNPII SKLYQGGPGG GGSSGGPTIE EVD 633
    Length:633
    Mass (Da):69,642
    Last modified:July 27, 2011 - v2
    Checksum:i6F65773C7EFFA69F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711A → R in AAA37859. (PubMed:3405224)Curated
    Sequence conflicti222 – 2221V → L in AAA37859. (PubMed:3405224)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20567 Genomic DNA. Translation: AAA37859.1.
    CH466526 Genomic DNA. Translation: EDL36460.1.
    BC004714 mRNA. Translation: AAH04714.1.
    BC052350 mRNA. Translation: AAH52350.1.
    CCDSiCCDS25993.1.
    PIRiS10859.
    RefSeqiNP_001002012.1. NM_001002012.1.
    NP_032327.2. NM_008301.4.
    XP_006515547.1. XM_006515484.1.
    UniGeneiMm.296181.

    Genome annotation databases

    EnsembliENSMUST00000080449; ENSMUSP00000079306; ENSMUSG00000059970.
    GeneIDi15512.
    KEGGimmu:15512.
    UCSCiuc007nyf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20567 Genomic DNA. Translation: AAA37859.1 .
    CH466526 Genomic DNA. Translation: EDL36460.1 .
    BC004714 mRNA. Translation: AAH04714.1 .
    BC052350 mRNA. Translation: AAH52350.1 .
    CCDSi CCDS25993.1.
    PIRi S10859.
    RefSeqi NP_001002012.1. NM_001002012.1.
    NP_032327.2. NM_008301.4.
    XP_006515547.1. XM_006515484.1.
    UniGenei Mm.296181.

    3D structure databases

    ProteinModelPortali P17156.
    SMRi P17156. Positions 3-613.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200453. 14 interactions.
    DIPi DIP-42071N.
    IntActi P17156. 5 interactions.
    MINTi MINT-1207820.
    STRINGi 10090.ENSMUSP00000079306.

    PTM databases

    PhosphoSitei P17156.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00331546.
    P17156.
    UCD-2DPAGE P17156.

    Proteomic databases

    MaxQBi P17156.
    PaxDbi P17156.
    PRIDEi P17156.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000080449 ; ENSMUSP00000079306 ; ENSMUSG00000059970 .
    GeneIDi 15512.
    KEGGi mmu:15512.
    UCSCi uc007nyf.1. mouse.

    Organism-specific databases

    CTDi 3306.
    MGIi MGI:96243. Hspa2.

    Phylogenomic databases

    eggNOGi COG0443.
    GeneTreei ENSGT00750000117237.
    HOGENOMi HOG000228135.
    HOVERGENi HBG051845.
    InParanoidi Q99KD7.
    KOi K03283.
    OMAi EAYLGCK.
    OrthoDBi EOG7PCJGF.
    TreeFami TF105042.

    Enzyme and pathway databases

    Reactomei REACT_198626. Meiotic synapsis.
    REACT_75800. Meiotic Synapsis.

    Miscellaneous databases

    NextBioi 288426.
    PROi P17156.
    SOURCEi Search...

    Gene expression databases

    Bgeei P17156.
    CleanExi MM_HSPA2.
    Genevestigatori P17156.

    Family and domain databases

    Gene3Di 1.20.1270.10. 1 hit.
    2.60.34.10. 1 hit.
    InterProi IPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR029047. HSP70_peptide-bd.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 1 hit.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    SUPFAMi SSF100920. SSF100920. 1 hit.
    SSF100934. SSF100934. 1 hit.
    PROSITEi PS00297. HSP70_1. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and sequence analysis of a new member of the mouse HSP70 gene family and characterization of its unique cellular and developmental pattern of expression in the male germ line."
      Zakeri Z.F., Wolgemuth D.J., Hunt C.R.
      Mol. Cell. Biol. 8:2925-2932(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor and Testis.
    4. "A novel germ cell-specific protein, SHIP1, forms a complex with chromatin remodeling activity during spermatogenesis."
      Choi E., Han C., Park I., Lee B., Jin S., Choi H., Kim do H., Park Z.Y., Eddy E.M., Cho C.
      J. Biol. Chem. 283:35283-35294(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZNF541.
    5. "A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa."
      Chung J.J., Navarro B., Krapivinsky G., Krapivinsky L., Clapham D.E.
      Nat. Commun. 2:153-153(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CATSPER COMPLEX.
      Strain: C57BL/6.
    6. "RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly."
      Lo J.C., Jamsai D., O'Connor A.E., Borg C., Clark B.J., Whisstock J.C., Field M.C., Adams V., Ishikawa T., Aitken R.J., Whittle B., Goodnow C.C., Ormandy C.J., O'Bryan M.K.
      PLoS Genet. 8:E1002969-E1002969(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RABL2, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiHSP72_MOUSE
    AccessioniPrimary (citable) accession number: P17156
    Secondary accession number(s): Q99KD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3