P17149 (PRIM_HCMVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA primase UL70
EC=2.7.7.-

Gene names

Name:

UL70

Organism

Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) [Complete proteome]

Taxonomic identifier

10360 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Betaherpesvirinae › Cytomegalovirus › Human cytomegalovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase presumably elongates using dNTPs. The UL70 subunit bears the primase activity By similarity.
Subunit structure	Interacts with host SNAPIN By similarity. Associates with the helicase-primase complex composed of the primase UL70, the helicase UL105 and the primase-associated factor UL102. Ref.4
Subcellular location	Host nucleus By similarity.
Sequence similarities	Belongs to the herpesviridae UL52 family.
Sequence caution	The sequence CAA35386.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA replication Host-virus interaction
Cellular component	Host nucleus
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: GOC virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA primase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	DNA primase UL70		PRO_0000116115
Regions
	Compositional bias	585 – 588	4	Poly-Asp

Sequences

	Sequence		Length	Mass (Da)
	P17149 [UniParc]. Last modified February 15, 2005. Version 2. Checksum: B64BFF01BF1D0A2D	FASTA	946	107,826
	10 20 30 40 50 60 MTLVLFATEY DSAHIVANVL SQTPTDHCVF PLLVKHQVSR RVYFCLQTQK CSDSRRVAPV 70 80 90 100 110 120 FAVNNETLQL SRYLAARQPI PLSALIASLD EAETQPLYRH LFRTPVLSPE HGGEVREFKH 130 140 150 160 170 180 LVYFHHAAVL RHLNQVFLCP TSPSWFISVF GHTEGQVLLT MAYYLFEGQY STISTVEEYV 190 200 210 220 230 240 RSFCTRDLGT IIPTHASMGE FARLLLGSPF RQRVSAFVAY AVARNRRDYT ELEQVDTQIN 250 260 270 280 290 300 AFRERARLPD TVCVHYVYLA YRTALARARL LEYRRVVAYD ADAAPEAQCT REPGFLGRRL 310 320 330 340 350 360 STELLDVMQK YFSLDNFLHD YVETHLLRLD ESPHSATSPH GLGLAGYGGR IDGTHLAGFF 370 380 390 400 410 420 GTSTQLARQL ERINTLSESV FSPLERSLSG LLRLCASLRT AQTYTTGTLT RYSQRRYLLP 430 440 450 460 470 480 EPALAPLLER PLPVYRVHLP NDQHVFCAVA SETWHRSLFP RDLLRHVPDS RFSDEALTET 490 500 510 520 530 540 VWLHDDDVAS TSPETQFYYT RHEVFNERLP VFNFVADFDL RLRDGVSGLA RHTVFELCRG 550 560 570 580 590 600 LRRVWMTVWA SLFGYTHPDR HPVYFFKSAC PPNSVPVDAA GAPFDDDDYL DYRDERDTEE 610 620 630 640 650 660 DEDGKEDKNN VPDNGVFQKT TSSVDTSPPY CRCKGKLGLR IITPFPACTV AVHPSVLRAV 670 680 690 700 710 720 AQVLNHAVCL DAELHTLLDP ISHPESSLDT GIYHHGRSVR LPYMYKMDQD DGYFMHRRLL 730 740 750 760 770 780 PLFIVPDAYR EHPLGFVRAQ LDLRNLLHHH PPHDLPALPL SPPPRVILSV RDKICPSTEA 790 800 810 820 830 840 NFIETRSLNV TRYRRRGLTE VLAYHLYGGD GATAAAISDT DLQRLVVTRV WPPLLEHLTQ 850 860 870 880 890 900 HYEPHVSEQF TAPHVLLFQP HGACCVAVKR RDGARTRDFR CLNYTHRNPQ ETVQVFIDLR 910 920 930 940 TEHSYALWAS LWSRCFTKKC HSNAKNVHIS IKIRPPDAPV PPATAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169." Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G. Curr. Top. Microbiol. Immunol. 154:125-169(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome." Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:17-28(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION.
[3]	Erratum Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:1053-1053(2003)
[4]	"Interactions between human cytomegalovirus helicase-primase proteins." McMahon T.P., Anders D.G. Virus Res. 86:39-52(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UL102 AND UL105.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17403 Genomic DNA. Translation: CAA35386.1. Different initiation. BK000394 Genomic DNA. Translation: DAA00166.1.
PIR	S09834.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-240904.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004340. DNA_primase_UL52/UL70_Herpvir. [Graphical view]
Pfam	PF03121. Herpes_UL52. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRIM_HCMVA

Accession

Primary (citable) accession number: P17149
Secondary accession number(s): Q7M6M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	February 15, 2005
Last modified:	April 3, 2013
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program