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Protein

DNA primase

Gene

UL70

Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase initiates primer synthesis and thereby produces large amount of short RNA primers on the lagging strand that the polymerase elongates using dNTPs.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei517Essential for primase activityUniRule annotation1
Sitei519Essential for primase activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri881 – 920CHC2-typeUniRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

  • bidirectional double-stranded viral DNA replication Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processDNA replication, Host-virus interaction
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
Gene namesi
Name:UL70
OrganismiHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri10360 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008992 Componenti: Genome
  • UP000008991 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

  • Note: Requires the presence of the primase associated factor to properly localize in the host cell nucleus.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001161151 – 946DNA primaseAdd BLAST946

Proteomic databases

PRIDEiP17149.

Interactioni

Subunit structurei

Associates with the helicase and the primase-associated factor to form the helicase-primase factor (By similarity).UniRule annotation1 Publication

Protein-protein interaction databases

MINTiMINT-240904.

Structurei

3D structure databases

ProteinModelPortaliP17149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi585 – 588Poly-Asp4

Sequence similaritiesi

Belongs to the herpesviridae DNA primase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri881 – 920CHC2-typeUniRule annotationAdd BLAST40

Keywords - Domaini

Zinc-finger

Phylogenomic databases

OrthoDBiVOG0900001H.

Family and domain databases

HAMAPiMF_04011. HSV_PRIM. 1 hit.
InterProiView protein in InterPro
IPR033685. HSV_PRIM.

Sequencei

Sequence statusi: Complete.

P17149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLVLFATEY DSAHIVANVL SQTPTDHCVF PLLVKHQVSR RVYFCLQTQK
60 70 80 90 100
CSDSRRVAPV FAVNNETLQL SRYLAARQPI PLSALIASLD EAETQPLYRH
110 120 130 140 150
LFRTPVLSPE HGGEVREFKH LVYFHHAAVL RHLNQVFLCP TSPSWFISVF
160 170 180 190 200
GHTEGQVLLT MAYYLFEGQY STISTVEEYV RSFCTRDLGT IIPTHASMGE
210 220 230 240 250
FARLLLGSPF RQRVSAFVAY AVARNRRDYT ELEQVDTQIN AFRERARLPD
260 270 280 290 300
TVCVHYVYLA YRTALARARL LEYRRVVAYD ADAAPEAQCT REPGFLGRRL
310 320 330 340 350
STELLDVMQK YFSLDNFLHD YVETHLLRLD ESPHSATSPH GLGLAGYGGR
360 370 380 390 400
IDGTHLAGFF GTSTQLARQL ERINTLSESV FSPLERSLSG LLRLCASLRT
410 420 430 440 450
AQTYTTGTLT RYSQRRYLLP EPALAPLLER PLPVYRVHLP NDQHVFCAVA
460 470 480 490 500
SETWHRSLFP RDLLRHVPDS RFSDEALTET VWLHDDDVAS TSPETQFYYT
510 520 530 540 550
RHEVFNERLP VFNFVADFDL RLRDGVSGLA RHTVFELCRG LRRVWMTVWA
560 570 580 590 600
SLFGYTHPDR HPVYFFKSAC PPNSVPVDAA GAPFDDDDYL DYRDERDTEE
610 620 630 640 650
DEDGKEDKNN VPDNGVFQKT TSSVDTSPPY CRCKGKLGLR IITPFPACTV
660 670 680 690 700
AVHPSVLRAV AQVLNHAVCL DAELHTLLDP ISHPESSLDT GIYHHGRSVR
710 720 730 740 750
LPYMYKMDQD DGYFMHRRLL PLFIVPDAYR EHPLGFVRAQ LDLRNLLHHH
760 770 780 790 800
PPHDLPALPL SPPPRVILSV RDKICPSTEA NFIETRSLNV TRYRRRGLTE
810 820 830 840 850
VLAYHLYGGD GATAAAISDT DLQRLVVTRV WPPLLEHLTQ HYEPHVSEQF
860 870 880 890 900
TAPHVLLFQP HGACCVAVKR RDGARTRDFR CLNYTHRNPQ ETVQVFIDLR
910 920 930 940
TEHSYALWAS LWSRCFTKKC HSNAKNVHIS IKIRPPDAPV PPATAV
Length:946
Mass (Da):107,826
Last modified:February 15, 2005 - v2
Checksum:iB64BFF01BF1D0A2D
GO

Sequence cautioni

The sequence CAA35386 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17403 Genomic DNA. Translation: CAA35386.1. Different initiation.
BK000394 Genomic DNA. Translation: DAA00166.1.
PIRiS09834.

Similar proteinsi

Entry informationi

Entry nameiPRIM_HCMVA
AccessioniPrimary (citable) accession number: P17149
Secondary accession number(s): Q7M6M2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 15, 2005
Last modified: May 10, 2017
This is version 61 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families