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P17121 (SAC7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTPase-activating protein SAC7
Gene names
Name:SAC7
Ordered Locus Names:YDR389W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length654 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase-activating protein for RHO1. Must be involved in the normal assembly or function or both of actin. Plays an essential role only at low temperatures. Ref.4

Subcellular location

Cytoplasm › cytoskeleton By similarity.

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Contains 1 Rho-GAP domain.

Sequence caution

The sequence AAA35016.1 differs from that shown. Reason: Frameshift at positions 201 and 498.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 654654GTPase-activating protein SAC7
PRO_0000056764

Regions

Domain134 – 393260Rho-GAP

Amino acid modifications

Modified residue371Phosphoserine Ref.9
Modified residue461Phosphoserine Ref.6 Ref.7
Modified residue991Phosphoserine Ref.8 Ref.9
Modified residue4351Phosphoserine Ref.9
Modified residue4911Phosphoserine Ref.6 Ref.9
Modified residue6001Phosphoserine Ref.9
Modified residue6321Phosphoserine Ref.9
Modified residue6481Phosphoserine Ref.8
Modified residue6501Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P17121 [UniParc].

Last modified February 12, 2003. Version 2.
Checksum: 50FD33872D13BBFD

FASTA65473,629
        10         20         30         40         50         60 
MPNNTLKQGS KIENVSPSKG HVPSFWKQFI NNPKSMSSEN ITVPRSPTSL SRNAQPTTLK 

        70         80         90        100        110        120 
RPPLSSRPYS YNTPTKDRKS FSKSAKQNNN NNNANSGTSP HAEFKNYRDM FLSNRNGFTG 

       130        140        150        160        170        180 
RVFGVTLAES LSVASAEVIV QSELVSFGRI PIVVAKCGAY LKANGLETSG IFRIAGNGKR 

       190        200        210        220        230        240 
VKALQYIFSS PPDYGTKFND WETYTVHDVA SLLRRYLNNL AEPLIPLSLY EQFRNPLRSR 

       250        260        270        280        290        300 
PRILRHMLTH EVSHPNANKT NNVTVKSSRQ NYNDDGANDG DIEKEDAKDD EEKRRRKIRH 

       310        320        330        340        350        360 
KRRLTRDIRA AIKEYEELFV TLSNDTKQLT IYLLDLLSLF ARQSQFNLMS GRNLAAIFQP 

       370        380        390        400        410        420 
SILSHPQHDM DPKEYELSRL VVEFLIEYSY KLLPHLLKLA KREQQERLST ENKKNNGDKQ 

       430        440        450        460        470        480 
KTDPIEIPKI TSSDSPPIVS SNKNPPAIDN NNKLDHTTLS PISTSIPENS SDLQTSKMLK 

       490        500        510        520        530        540 
PPKQRRPHSK SFGSTPVPPD VIASNKRRTS LFPWLHKPGI LSDTGDNGDL TATEAEGDDY 

       550        560        570        580        590        600 
EEENVDPYGQ SPSSVHSGSL PKQHYLPIPR MNRSLSGNST NSSFNTRPIS MILTSGNDNS 

       610        620        630        640        650 
ADQLELLSNT HSNNERSNAL PLTEDDGDER NSRSRKRESW FQRLTSRSGS ANRA

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Null alleles of SAC7 suppress temperature-sensitive actin mutations in Saccharomyces cerevisiae."
Dunn T.M., Shortle D.
Mol. Cell. Biol. 10:2308-2314(1990) [PubMed: 2183030] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-654.
[4]"The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2."
Schmidt A., Bickle M., Beck T., Hall M.N.
Cell 88:531-542(1997) [PubMed: 9038344] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46 AND SER-491, MASS SPECTROMETRY.
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-46, MASS SPECTROMETRY.
[8]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-99; SER-648 AND SER-650, MASS SPECTROMETRY.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37; SER-99; SER-435; SER-491; SER-600 AND SER-632, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U32274 Genomic DNA. Translation: AAB64831.1.
M32335 Genomic DNA. Translation: AAA35016.1. Frameshift.
BK006938 Genomic DNA. Translation: DAA12233.1.
PIRS69673.
RefSeqNP_010677.1. NM_001180697.1.

3D structure databases

ProteinModelPortalP17121.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1010N.
IntActP17121. 1 interaction.
MINTMINT-427802.
STRINGP17121.

Proteomic databases

PeptideAtlasP17121.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR389W; YDR389W; YDR389W.
GeneID851997.
KEGGsce:YDR389W.
NMPDRfig|4932.3.peg.1449.

Organism-specific databases

CYGDYDR389w.
SGDS000002797. SAC7.

Phylogenomic databases

eggNOGfuNOG06782.
GeneTreeEFGT00050000000812.
HOGENOMHBG203279.
OMAVSHPNAN.
OrthoDBEOG49GPR0.

Gene expression databases

ArrayExpressP17121.
GenevestigatorP17121.
GermOnlineYDR389W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
Gene3DG3DSA:1.10.555.10. RhoGAP. 2 hits.
PfamPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMSSF48350. Rho_GAP. 1 hit.
PROSITEPS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970175.

Entry information

Entry nameSAC7_YEAST
AccessionPrimary (citable) accession number: P17121
Secondary accession number(s): D6VT23, Q04163
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 12, 2003
Last modified: December 14, 2011
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents