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Protein

Kinesin-like protein KAR3

Gene

KAR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for yeast nuclear fusion during mating. KAR3 is a bifunctional protein having a kinesin-like motor domain joined to a distinct microtubule binding domain. It may mediate microtubule sliding during nuclear fusion and possibly mitosis. May interact with spindle microtubules to produce an inwardly directed force acting upon the poles. KAR3 function antagonizes CIP8 and KIP1 outward force action. KAR3 motor activity is directed toward the microtubule's minus end.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi474 – 481ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, minus-end-directed Source: SGD
  • microtubule binding Source: SGD
  • protein heterodimerization activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • karyogamy involved in conjugation with cellular fusion Source: SGD
  • meiotic nuclear division Source: SGD
  • mitotic nuclear division Source: SGD
  • mitotic sister chromatid cohesion Source: SGD
  • nuclear migration involved in conjugation with cellular fusion Source: SGD
  • regulation of mitotic spindle organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Karyogamy

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34276-MONOMER.
BRENDAi3.6.4.5. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KAR3
Alternative name(s):
Nuclear fusion protein
Gene namesi
Name:KAR3
Ordered Locus Names:YPR141C
ORF Names:P9659.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR141C.
SGDiS000006345. KAR3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic microtubule Source: SGD
  • nucleus Source: UniProtKB-SubCell
  • spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi479G → E: Poisons nuclear fusion. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001253911 – 729Kinesin-like protein KAR3Add BLAST729

Proteomic databases

MaxQBiP17119.
PRIDEiP17119.

PTM databases

iPTMnetiP17119.

Expressioni

Inductioni

By alpha factor.

Interactioni

Subunit structurei

Interacts with CIK1 and VIK1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SMC1P329084EBI-9499,EBI-17402
VIK1Q120454EBI-9499,EBI-38784

GO - Molecular functioni

  • microtubule binding Source: SGD
  • protein heterodimerization activity Source: SGD

Protein-protein interaction databases

BioGridi36310. 288 interactors.
DIPiDIP-75N.
IntActiP17119. 8 interactors.
MINTiMINT-435688.

Structurei

Secondary structure

1729
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi348 – 384Combined sources37
Beta strandi386 – 393Combined sources8
Turni398 – 400Combined sources3
Beta strandi406 – 410Combined sources5
Turni415 – 418Combined sources4
Beta strandi419 – 426Combined sources8
Helixi427 – 429Combined sources3
Beta strandi433 – 442Combined sources10
Helixi448 – 459Combined sources12
Helixi460 – 464Combined sources5
Beta strandi468 – 473Combined sources6
Helixi480 – 485Combined sources6
Turni487 – 489Combined sources3
Helixi491 – 506Combined sources16
Helixi507 – 509Combined sources3
Beta strandi512 – 523Combined sources12
Beta strandi526 – 529Combined sources4
Beta strandi549 – 552Combined sources4
Turni553 – 556Combined sources4
Beta strandi557 – 560Combined sources4
Beta strandi566 – 570Combined sources5
Helixi571 – 573Combined sources3
Helixi574 – 581Combined sources8
Helixi596 – 598Combined sources3
Beta strandi599 – 610Combined sources12
Turni612 – 614Combined sources3
Beta strandi617 – 626Combined sources10
Helixi635 – 637Combined sources3
Helixi640 – 663Combined sources24
Turni668 – 670Combined sources3
Helixi675 – 677Combined sources3
Helixi679 – 688Combined sources10
Beta strandi693 – 700Combined sources8
Helixi704 – 706Combined sources3
Helixi707 – 720Combined sources14
Turni723 – 725Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F9TX-ray1.50A372-729[»]
1F9UX-ray1.70A383-729[»]
1F9VX-ray1.30A383-729[»]
1F9WX-ray2.50A/B383-729[»]
3KARX-ray2.30A385-729[»]
4ETPX-ray2.30A348-729[»]
ProteinModelPortaliP17119.
SMRiP17119.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17119.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini386 – 723Kinesin motorPROSITE-ProRule annotationAdd BLAST338

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 109GlobularAdd BLAST109

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili110 – 357Sequence analysisAdd BLAST248

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. NCD subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074610.
HOGENOMiHOG000116164.
InParanoidiP17119.
KOiK10405.
OMAiCEFIEIY.
OrthoDBiEOG092C2D4G.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 3 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ
60 70 80 90 100
RTDIHRHSLA SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE
110 120 130 140 150
NVNELNRTQA ILFEKKATLD LLKDELTETK EKINAVNLKF ETLREEKIKI
160 170 180 190 200
EQQLNLKNNE LISIKEEFLS KKQFMNEGHE IHLKQLAASN KKELKQMENE
210 220 230 240 250
YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST LQEMLNDVEQ
260 270 280 290 300
KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK
310 320 330 340 350
KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE
360 370 380 390 400
YIKDTELGMK ELNEILIKEE TVRRTLHNEL QELRGNIRVY CRIRPALKNL
410 420 430 440 450
ENSDTSLINV NEFDDNSGVQ SMEVTKIQNT AQVHEFKFDK IFDQQDTNVD
460 470 480 490 500
VFKEVGQLVQ SSLDGYNVCI FAYGQTGSGK TFTMLNPGDG IIPSTISHIF
510 520 530 540 550
NWINKLKTKG WDYKVNCEFI EIYNENIVDL LRSDNNNKED TSIGLKHEIR
560 570 580 590 600
HDQETKTTTI TNVTSCKLES EEMVEIILKK ANKLRSTAST ASNEHSSRSH
610 620 630 640 650
SIFIIHLSGS NAKTGAHSYG TLNLVDLAGS ERINVSQVVG DRLRETQNIN
660 670 680 690 700
KSLSCLGDVI HALGQPDSTK RHIPFRNSKL TYLLQYSLTG DSKTLMFVNI
710 720
SPSSSHINET LNSLRFASKV NSTRLVSRK
Length:729
Mass (Da):84,004
Last modified:August 1, 1990 - v1
Checksum:iBAEF98BC783ABDB9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti378N → K in KAR3-894. 1
Natural varianti462S → L in KAR3-891. 1
Natural varianti521E → D in KAR3-893. 1
Natural varianti550R → S in KAR3-899. 1
Natural varianti558T → A in KAR3-8912. 1
Natural varianti650N → K in KAR3-898. 1
Natural varianti659V → L in KAR3-897. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31719 mRNA. Translation: AAA34715.1.
U40829 Genomic DNA. Translation: AAB68281.1.
BK006949 Genomic DNA. Translation: DAA11555.1.
PIRiA34796.
RefSeqiNP_015467.1. NM_001184238.1.

Genome annotation databases

EnsemblFungiiYPR141C; YPR141C; YPR141C.
GeneIDi856263.
KEGGisce:YPR141C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31719 mRNA. Translation: AAA34715.1.
U40829 Genomic DNA. Translation: AAB68281.1.
BK006949 Genomic DNA. Translation: DAA11555.1.
PIRiA34796.
RefSeqiNP_015467.1. NM_001184238.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F9TX-ray1.50A372-729[»]
1F9UX-ray1.70A383-729[»]
1F9VX-ray1.30A383-729[»]
1F9WX-ray2.50A/B383-729[»]
3KARX-ray2.30A385-729[»]
4ETPX-ray2.30A348-729[»]
ProteinModelPortaliP17119.
SMRiP17119.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36310. 288 interactors.
DIPiDIP-75N.
IntActiP17119. 8 interactors.
MINTiMINT-435688.

PTM databases

iPTMnetiP17119.

Proteomic databases

MaxQBiP17119.
PRIDEiP17119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR141C; YPR141C; YPR141C.
GeneIDi856263.
KEGGisce:YPR141C.

Organism-specific databases

EuPathDBiFungiDB:YPR141C.
SGDiS000006345. KAR3.

Phylogenomic databases

GeneTreeiENSGT00550000074610.
HOGENOMiHOG000116164.
InParanoidiP17119.
KOiK10405.
OMAiCEFIEIY.
OrthoDBiEOG092C2D4G.

Enzyme and pathway databases

BioCyciYEAST:G3O-34276-MONOMER.
BRENDAi3.6.4.5. 984.

Miscellaneous databases

EvolutionaryTraceiP17119.
PROiP17119.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 3 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAR3_YEAST
AccessioniPrimary (citable) accession number: P17119
Secondary accession number(s): D6W4D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 2, 2016
This is version 172 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

KAR3 contains two globular domains separated by an alpha-helical coiled coil. The N-terminal portion of KAR3 contains a microtubule association domain distinct from the kinesin-like C-terminal domain.
Present with 3250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.