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P17119

- KAR3_YEAST

UniProt

P17119 - KAR3_YEAST

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Protein
Kinesin-like protein KAR3
Gene
KAR3, YPR141C, P9659.16
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for yeast nuclear fusion during mating. KAR3 is a bifunctional protein having a kinesin-like motor domain joined to a distinct microtubule binding domain. It may mediate microtubule sliding during nuclear fusion and possibly mitosis. May interact with spindle microtubules to produce an inwardly directed force acting upon the poles. KAR3 function antagonizes CIP8 and KIP1 outward force action. KAR3 motor activity is directed toward the microtubule's minus end.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi474 – 4818ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. microtubule binding Source: SGD
  3. minus-end-directed microtubule motor activity Source: SGD
  4. protein binding Source: IntAct
  5. protein heterodimerization activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. karyogamy involved in conjugation with cellular fusion Source: SGD
  2. meiotic nuclear division Source: SGD
  3. metabolic process Source: GOC
  4. mitotic nuclear division Source: SGD
  5. mitotic sister chromatid cohesion Source: SGD
  6. nuclear migration involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Karyogamy

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34276-MONOMER.
ReactomeiREACT_189062. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KAR3
Alternative name(s):
Nuclear fusion protein
Gene namesi
Name:KAR3
Ordered Locus Names:YPR141C
ORF Names:P9659.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR141c.
SGDiS000006345. KAR3.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body. Nucleus. Cytoplasmcytoskeleton
Note: Cytoplasmic microtubules.2 Publications

GO - Cellular componenti

  1. cytoplasmic microtubule Source: SGD
  2. kinesin complex Source: InterPro
  3. nucleus Source: UniProtKB-SubCell
  4. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi479 – 4791G → E: Poisons nuclear fusion.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Kinesin-like protein KAR3
PRO_0000125391Add
BLAST

Proteomic databases

MaxQBiP17119.
PaxDbiP17119.
PeptideAtlasiP17119.

Expressioni

Inductioni

By alpha factor.

Gene expression databases

GenevestigatoriP17119.

Interactioni

Subunit structurei

Interacts with CIK1 and VIK1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SMC1P329084EBI-9499,EBI-17402
VIK1Q120454EBI-9499,EBI-38784

Protein-protein interaction databases

BioGridi36310. 285 interactions.
DIPiDIP-75N.
IntActiP17119. 8 interactions.
MINTiMINT-435688.
STRINGi4932.YPR141C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi348 – 38437
Beta strandi386 – 3938
Turni398 – 4003
Beta strandi406 – 4105
Turni415 – 4184
Beta strandi419 – 4268
Helixi427 – 4293
Beta strandi433 – 44210
Helixi448 – 45912
Helixi460 – 4645
Beta strandi468 – 4736
Helixi480 – 4856
Turni487 – 4893
Helixi491 – 50616
Helixi507 – 5093
Beta strandi512 – 52312
Beta strandi526 – 5294
Beta strandi549 – 5524
Turni553 – 5564
Beta strandi557 – 5604
Beta strandi566 – 5705
Helixi571 – 5733
Helixi574 – 5818
Helixi596 – 5983
Beta strandi599 – 61012
Turni612 – 6143
Beta strandi617 – 62610
Helixi635 – 6373
Helixi640 – 66324
Turni668 – 6703
Helixi675 – 6773
Helixi679 – 68810
Beta strandi693 – 7008
Helixi704 – 7063
Helixi707 – 72014
Turni723 – 7253

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F9TX-ray1.50A372-729[»]
1F9UX-ray1.70A383-729[»]
1F9VX-ray1.30A383-729[»]
1F9WX-ray2.50A/B383-729[»]
3KARX-ray2.30A385-729[»]
4ETPX-ray2.30A348-729[»]
ProteinModelPortaliP17119.
SMRiP17119. Positions 343-723.

Miscellaneous databases

EvolutionaryTraceiP17119.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini386 – 723338Kinesin motor
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 109109Globular
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili110 – 357248 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00550000074610.
HOGENOMiHOG000116164.
KOiK10405.
OMAiCKLESEE.
OrthoDBiEOG7RRFGF.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17119-1 [UniParc]FASTAAdd to Basket

« Hide

MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ    50
RTDIHRHSLA SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE 100
NVNELNRTQA ILFEKKATLD LLKDELTETK EKINAVNLKF ETLREEKIKI 150
EQQLNLKNNE LISIKEEFLS KKQFMNEGHE IHLKQLAASN KKELKQMENE 200
YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST LQEMLNDVEQ 250
KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK 300
KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE 350
YIKDTELGMK ELNEILIKEE TVRRTLHNEL QELRGNIRVY CRIRPALKNL 400
ENSDTSLINV NEFDDNSGVQ SMEVTKIQNT AQVHEFKFDK IFDQQDTNVD 450
VFKEVGQLVQ SSLDGYNVCI FAYGQTGSGK TFTMLNPGDG IIPSTISHIF 500
NWINKLKTKG WDYKVNCEFI EIYNENIVDL LRSDNNNKED TSIGLKHEIR 550
HDQETKTTTI TNVTSCKLES EEMVEIILKK ANKLRSTAST ASNEHSSRSH 600
SIFIIHLSGS NAKTGAHSYG TLNLVDLAGS ERINVSQVVG DRLRETQNIN 650
KSLSCLGDVI HALGQPDSTK RHIPFRNSKL TYLLQYSLTG DSKTLMFVNI 700
SPSSSHINET LNSLRFASKV NSTRLVSRK 729
Length:729
Mass (Da):84,004
Last modified:August 1, 1990 - v1
Checksum:iBAEF98BC783ABDB9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti378 – 3781N → K in KAR3-894.
Natural varianti462 – 4621S → L in KAR3-891.
Natural varianti521 – 5211E → D in KAR3-893.
Natural varianti550 – 5501R → S in KAR3-899.
Natural varianti558 – 5581T → A in KAR3-8912.
Natural varianti650 – 6501N → K in KAR3-898.
Natural varianti659 – 6591V → L in KAR3-897.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31719 mRNA. Translation: AAA34715.1.
U40829 Genomic DNA. Translation: AAB68281.1.
BK006949 Genomic DNA. Translation: DAA11555.1.
PIRiA34796.
RefSeqiNP_015467.1. NM_001184238.1.

Genome annotation databases

EnsemblFungiiYPR141C; YPR141C; YPR141C.
GeneIDi856263.
KEGGisce:YPR141C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31719 mRNA. Translation: AAA34715.1 .
U40829 Genomic DNA. Translation: AAB68281.1 .
BK006949 Genomic DNA. Translation: DAA11555.1 .
PIRi A34796.
RefSeqi NP_015467.1. NM_001184238.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1F9T X-ray 1.50 A 372-729 [» ]
1F9U X-ray 1.70 A 383-729 [» ]
1F9V X-ray 1.30 A 383-729 [» ]
1F9W X-ray 2.50 A/B 383-729 [» ]
3KAR X-ray 2.30 A 385-729 [» ]
4ETP X-ray 2.30 A 348-729 [» ]
ProteinModelPortali P17119.
SMRi P17119. Positions 343-723.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36310. 285 interactions.
DIPi DIP-75N.
IntActi P17119. 8 interactions.
MINTi MINT-435688.
STRINGi 4932.YPR141C.

Proteomic databases

MaxQBi P17119.
PaxDbi P17119.
PeptideAtlasi P17119.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR141C ; YPR141C ; YPR141C .
GeneIDi 856263.
KEGGi sce:YPR141C.

Organism-specific databases

CYGDi YPR141c.
SGDi S000006345. KAR3.

Phylogenomic databases

eggNOGi COG5059.
GeneTreei ENSGT00550000074610.
HOGENOMi HOG000116164.
KOi K10405.
OMAi CKLESEE.
OrthoDBi EOG7RRFGF.

Enzyme and pathway databases

BioCyci YEAST:G3O-34276-MONOMER.
Reactomei REACT_189062. Kinesins.

Miscellaneous databases

EvolutionaryTracei P17119.
NextBioi 981560.
PROi P17119.

Gene expression databases

Genevestigatori P17119.

Family and domain databases

Gene3Di 3.40.850.10. 1 hit.
InterProi IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR24115. PTHR24115. 1 hit.
Pfami PF00225. Kinesin. 1 hit.
[Graphical view ]
PRINTSi PR00380. KINESINHEAVY.
SMARTi SM00129. KISc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "KAR3, a kinesin-related gene required for yeast nuclear fusion."
    Meluh P.B., Rose M.D.
    Cell 60:1029-1041(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: MY1124.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations."
    Hoyt M.A., He L., Totis L., Saunders W.S.
    Genetics 135:35-44(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANTS KAR3.
  5. "Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends."
    Endow S.A., Kang S.J., Satterwhite L.L., Rose M.D., Skeen V.P., Salmon E.D.
    EMBO J. 13:2708-2713(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p."
    Manning B.D., Barrett J.G., Wallace J.A., Granok H., Snyder M.
    J. Cell Biol. 144:1219-1233(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CIK1 AND VIK1, SUBCELLULAR LOCATION.
  7. "The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae."
    Shanks R.M.Q., Kamieniecki R.J., Dawson D.S.
    Genetics 159:939-951(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3-A resolution."
    Gulick A.M., Song H., Endow S.A., Rayment I.
    Biochemistry 37:1769-1776(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 383-729.

Entry informationi

Entry nameiKAR3_YEAST
AccessioniPrimary (citable) accession number: P17119
Secondary accession number(s): D6W4D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 3, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

KAR3 contains two globular domains separated by an alpha-helical coiled coil. The N-terminal portion of KAR3 contains a microtubule association domain distinct from the kinesin-like C-terminal domain.
Present with 3250 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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