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P17119 (KAR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 148. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein KAR3
Alternative name(s):
Nuclear fusion protein
Gene names
Name:KAR3
Ordered Locus Names:YPR141C
ORF Names:P9659.16
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for yeast nuclear fusion during mating. KAR3 is a bifunctional protein having a kinesin-like motor domain joined to a distinct microtubule binding domain. It may mediate microtubule sliding during nuclear fusion and possibly mitosis. May interact with spindle microtubules to produce an inwardly directed force acting upon the poles. KAR3 function antagonizes CIP8 and KIP1 outward force action. KAR3 motor activity is directed toward the microtubule's minus end. Ref.1 Ref.7

Subunit structure

Interacts with CIK1 and VIK1. Ref.6

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body. Nucleus. Cytoplasmcytoskeleton. Note: Cytoplasmic microtubules. Ref.6 Ref.7

Induction

By alpha factor.

Miscellaneous

KAR3 contains two globular domains separated by an alpha-helical coiled coil. The N-terminal portion of KAR3 contains a microtubule association domain distinct from the kinesin-like C-terminal domain.

Present with 3250 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. NCD subfamily.

Contains 1 kinesin motor domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Karyogamy
   Cellular componentCytoplasm
Cytoskeleton
Microtubule
Nucleus
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionMotor protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processkaryogamy involved in conjugation with cellular fusion

Inferred from mutant phenotype Ref.1. Source: SGD

meiotic nuclear division

Inferred from direct assay Ref.7. Source: SGD

metabolic process

Inferred from direct assay PubMed 8041770. Source: GOC

mitotic nuclear division

Inferred from genetic interaction PubMed 9201713. Source: SGD

mitotic sister chromatid cohesion

Inferred from mutant phenotype PubMed 14742714. Source: SGD

nuclear migration involved in conjugation with cellular fusion

Inferred from mutant phenotype PubMed 16380440PubMed 19386762Ref.1. Source: SGD

   Cellular_componentcytoplasmic microtubule

Inferred from direct assay Ref.1. Source: SGD

kinesin complex

Inferred from electronic annotation. Source: InterPro

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

spindle pole body

Inferred from direct assay Ref.7Ref.1PubMed 8106549. Source: SGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule binding

Inferred from direct assay PubMed 22734002. Source: SGD

minus-end-directed microtubule motor activity

Inferred from direct assay PubMed 8041770. Source: SGD

protein binding

Inferred from physical interaction Ref.6PubMed 11087867PubMed 14742714PubMed 17382884. Source: IntAct

protein heterodimerization activity

Inferred from physical interaction PubMed 16107877. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SMC1P329084EBI-9499,EBI-17402
VIK1Q120454EBI-9499,EBI-38784

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Kinesin-like protein KAR3
PRO_0000125391

Regions

Domain386 – 723338Kinesin motor
Nucleotide binding474 – 4818ATP By similarity
Region1 – 109109Globular
Coiled coil110 – 357248 Potential

Natural variations

Natural variant3781N → K in KAR3-894.
Natural variant4621S → L in KAR3-891.
Natural variant5211E → D in KAR3-893.
Natural variant5501R → S in KAR3-899.
Natural variant5581T → A in KAR3-8912.
Natural variant6501N → K in KAR3-898.
Natural variant6591V → L in KAR3-897.

Experimental info

Mutagenesis4791G → E: Poisons nuclear fusion.

Secondary structure

............................................................... 729
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P17119 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: BAEF98BC783ABDB9

FASTA72984,004
        10         20         30         40         50         60 
MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ RTDIHRHSLA 

        70         80         90        100        110        120 
SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE NVNELNRTQA ILFEKKATLD 

       130        140        150        160        170        180 
LLKDELTETK EKINAVNLKF ETLREEKIKI EQQLNLKNNE LISIKEEFLS KKQFMNEGHE 

       190        200        210        220        230        240 
IHLKQLAASN KKELKQMENE YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST 

       250        260        270        280        290        300 
LQEMLNDVEQ KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK 

       310        320        330        340        350        360 
KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE YIKDTELGMK 

       370        380        390        400        410        420 
ELNEILIKEE TVRRTLHNEL QELRGNIRVY CRIRPALKNL ENSDTSLINV NEFDDNSGVQ 

       430        440        450        460        470        480 
SMEVTKIQNT AQVHEFKFDK IFDQQDTNVD VFKEVGQLVQ SSLDGYNVCI FAYGQTGSGK 

       490        500        510        520        530        540 
TFTMLNPGDG IIPSTISHIF NWINKLKTKG WDYKVNCEFI EIYNENIVDL LRSDNNNKED 

       550        560        570        580        590        600 
TSIGLKHEIR HDQETKTTTI TNVTSCKLES EEMVEIILKK ANKLRSTAST ASNEHSSRSH 

       610        620        630        640        650        660 
SIFIIHLSGS NAKTGAHSYG TLNLVDLAGS ERINVSQVVG DRLRETQNIN KSLSCLGDVI 

       670        680        690        700        710        720 
HALGQPDSTK RHIPFRNSKL TYLLQYSLTG DSKTLMFVNI SPSSSHINET LNSLRFASKV 


NSTRLVSRK 

« Hide

References

« Hide 'large scale' references
[1]"KAR3, a kinesin-related gene required for yeast nuclear fusion."
Meluh P.B., Rose M.D.
Cell 60:1029-1041(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: MY1124.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations."
Hoyt M.A., He L., Totis L., Saunders W.S.
Genetics 135:35-44(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTANTS KAR3.
[5]"Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends."
Endow S.A., Kang S.J., Satterwhite L.L., Rose M.D., Skeen V.P., Salmon E.D.
EMBO J. 13:2708-2713(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p."
Manning B.D., Barrett J.G., Wallace J.A., Granok H., Snyder M.
J. Cell Biol. 144:1219-1233(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CIK1 AND VIK1, SUBCELLULAR LOCATION.
[7]"The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae."
Shanks R.M.Q., Kamieniecki R.J., Dawson D.S.
Genetics 159:939-951(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3-A resolution."
Gulick A.M., Song H., Endow S.A., Rayment I.
Biochemistry 37:1769-1776(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 383-729.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31719 mRNA. Translation: AAA34715.1.
U40829 Genomic DNA. Translation: AAB68281.1.
BK006949 Genomic DNA. Translation: DAA11555.1.
PIRA34796.
RefSeqNP_015467.1. NM_001184238.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1F9TX-ray1.50A372-729[»]
1F9UX-ray1.70A383-729[»]
1F9VX-ray1.30A383-729[»]
1F9WX-ray2.50A/B383-729[»]
3KARX-ray2.30A385-729[»]
4ETPX-ray2.30A348-729[»]
ProteinModelPortalP17119.
SMRP17119. Positions 343-723.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36310. 285 interactions.
DIPDIP-75N.
IntActP17119. 8 interactions.
MINTMINT-435688.
STRING4932.YPR141C.

Proteomic databases

MaxQBP17119.
PaxDbP17119.
PeptideAtlasP17119.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR141C; YPR141C; YPR141C.
GeneID856263.
KEGGsce:YPR141C.

Organism-specific databases

CYGDYPR141c.
SGDS000006345. KAR3.

Phylogenomic databases

eggNOGCOG5059.
GeneTreeENSGT00550000074610.
HOGENOMHOG000116164.
KOK10405.
OMACKLESEE.
OrthoDBEOG7RRFGF.

Enzyme and pathway databases

BioCycYEAST:G3O-34276-MONOMER.

Gene expression databases

GenevestigatorP17119.

Family and domain databases

Gene3D3.40.850.10. 1 hit.
InterProIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR24115. PTHR24115. 1 hit.
PfamPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP17119.
NextBio981560.
PROP17119.

Entry information

Entry nameKAR3_YEAST
AccessionPrimary (citable) accession number: P17119
Secondary accession number(s): D6W4D9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 9, 2014
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references