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P17119

- KAR3_YEAST

UniProt

P17119 - KAR3_YEAST

Protein

Kinesin-like protein KAR3

Gene

KAR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 150 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Essential for yeast nuclear fusion during mating. KAR3 is a bifunctional protein having a kinesin-like motor domain joined to a distinct microtubule binding domain. It may mediate microtubule sliding during nuclear fusion and possibly mitosis. May interact with spindle microtubules to produce an inwardly directed force acting upon the poles. KAR3 function antagonizes CIP8 and KIP1 outward force action. KAR3 motor activity is directed toward the microtubule's minus end.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi474 – 4818ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. microtubule binding Source: SGD
    3. minus-end-directed microtubule motor activity Source: SGD
    4. protein binding Source: IntAct
    5. protein heterodimerization activity Source: SGD

    GO - Biological processi

    1. karyogamy involved in conjugation with cellular fusion Source: SGD
    2. meiotic nuclear division Source: SGD
    3. metabolic process Source: GOC
    4. mitotic nuclear division Source: SGD
    5. mitotic sister chromatid cohesion Source: SGD
    6. nuclear migration involved in conjugation with cellular fusion Source: SGD

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Cell cycle, Cell division, Karyogamy

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34276-MONOMER.
    ReactomeiREACT_189062. Kinesins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-like protein KAR3
    Alternative name(s):
    Nuclear fusion protein
    Gene namesi
    Name:KAR3
    Ordered Locus Names:YPR141C
    ORF Names:P9659.16
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR141c.
    SGDiS000006345. KAR3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic microtubule Source: SGD
    2. kinesin complex Source: InterPro
    3. nucleus Source: UniProtKB-SubCell
    4. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi479 – 4791G → E: Poisons nuclear fusion.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 729729Kinesin-like protein KAR3PRO_0000125391Add
    BLAST

    Proteomic databases

    MaxQBiP17119.
    PaxDbiP17119.
    PeptideAtlasiP17119.

    Expressioni

    Inductioni

    By alpha factor.

    Gene expression databases

    GenevestigatoriP17119.

    Interactioni

    Subunit structurei

    Interacts with CIK1 and VIK1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SMC1P329084EBI-9499,EBI-17402
    VIK1Q120454EBI-9499,EBI-38784

    Protein-protein interaction databases

    BioGridi36310. 285 interactions.
    DIPiDIP-75N.
    IntActiP17119. 8 interactions.
    MINTiMINT-435688.
    STRINGi4932.YPR141C.

    Structurei

    Secondary structure

    1
    729
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi348 – 38437
    Beta strandi386 – 3938
    Turni398 – 4003
    Beta strandi406 – 4105
    Turni415 – 4184
    Beta strandi419 – 4268
    Helixi427 – 4293
    Beta strandi433 – 44210
    Helixi448 – 45912
    Helixi460 – 4645
    Beta strandi468 – 4736
    Helixi480 – 4856
    Turni487 – 4893
    Helixi491 – 50616
    Helixi507 – 5093
    Beta strandi512 – 52312
    Beta strandi526 – 5294
    Beta strandi549 – 5524
    Turni553 – 5564
    Beta strandi557 – 5604
    Beta strandi566 – 5705
    Helixi571 – 5733
    Helixi574 – 5818
    Helixi596 – 5983
    Beta strandi599 – 61012
    Turni612 – 6143
    Beta strandi617 – 62610
    Helixi635 – 6373
    Helixi640 – 66324
    Turni668 – 6703
    Helixi675 – 6773
    Helixi679 – 68810
    Beta strandi693 – 7008
    Helixi704 – 7063
    Helixi707 – 72014
    Turni723 – 7253

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1F9TX-ray1.50A372-729[»]
    1F9UX-ray1.70A383-729[»]
    1F9VX-ray1.30A383-729[»]
    1F9WX-ray2.50A/B383-729[»]
    3KARX-ray2.30A385-729[»]
    4ETPX-ray2.30A348-729[»]
    ProteinModelPortaliP17119.
    SMRiP17119. Positions 343-723.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17119.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini386 – 723338Kinesin motorPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 109109GlobularAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili110 – 357248Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. NCD subfamily.PROSITE-ProRule annotation
    Contains 1 kinesin motor domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5059.
    GeneTreeiENSGT00550000074610.
    HOGENOMiHOG000116164.
    KOiK10405.
    OMAiCKLESEE.
    OrthoDBiEOG7RRFGF.

    Family and domain databases

    Gene3Di3.40.850.10. 1 hit.
    InterProiIPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR24115. PTHR24115. 1 hit.
    PfamiPF00225. Kinesin. 1 hit.
    [Graphical view]
    PRINTSiPR00380. KINESINHEAVY.
    SMARTiSM00129. KISc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P17119-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ    50
    RTDIHRHSLA SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE 100
    NVNELNRTQA ILFEKKATLD LLKDELTETK EKINAVNLKF ETLREEKIKI 150
    EQQLNLKNNE LISIKEEFLS KKQFMNEGHE IHLKQLAASN KKELKQMENE 200
    YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST LQEMLNDVEQ 250
    KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK 300
    KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE 350
    YIKDTELGMK ELNEILIKEE TVRRTLHNEL QELRGNIRVY CRIRPALKNL 400
    ENSDTSLINV NEFDDNSGVQ SMEVTKIQNT AQVHEFKFDK IFDQQDTNVD 450
    VFKEVGQLVQ SSLDGYNVCI FAYGQTGSGK TFTMLNPGDG IIPSTISHIF 500
    NWINKLKTKG WDYKVNCEFI EIYNENIVDL LRSDNNNKED TSIGLKHEIR 550
    HDQETKTTTI TNVTSCKLES EEMVEIILKK ANKLRSTAST ASNEHSSRSH 600
    SIFIIHLSGS NAKTGAHSYG TLNLVDLAGS ERINVSQVVG DRLRETQNIN 650
    KSLSCLGDVI HALGQPDSTK RHIPFRNSKL TYLLQYSLTG DSKTLMFVNI 700
    SPSSSHINET LNSLRFASKV NSTRLVSRK 729
    Length:729
    Mass (Da):84,004
    Last modified:August 1, 1990 - v1
    Checksum:iBAEF98BC783ABDB9
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti378 – 3781N → K in KAR3-894.
    Natural varianti462 – 4621S → L in KAR3-891.
    Natural varianti521 – 5211E → D in KAR3-893.
    Natural varianti550 – 5501R → S in KAR3-899.
    Natural varianti558 – 5581T → A in KAR3-8912.
    Natural varianti650 – 6501N → K in KAR3-898.
    Natural varianti659 – 6591V → L in KAR3-897.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31719 mRNA. Translation: AAA34715.1.
    U40829 Genomic DNA. Translation: AAB68281.1.
    BK006949 Genomic DNA. Translation: DAA11555.1.
    PIRiA34796.
    RefSeqiNP_015467.1. NM_001184238.1.

    Genome annotation databases

    EnsemblFungiiYPR141C; YPR141C; YPR141C.
    GeneIDi856263.
    KEGGisce:YPR141C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31719 mRNA. Translation: AAA34715.1 .
    U40829 Genomic DNA. Translation: AAB68281.1 .
    BK006949 Genomic DNA. Translation: DAA11555.1 .
    PIRi A34796.
    RefSeqi NP_015467.1. NM_001184238.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1F9T X-ray 1.50 A 372-729 [» ]
    1F9U X-ray 1.70 A 383-729 [» ]
    1F9V X-ray 1.30 A 383-729 [» ]
    1F9W X-ray 2.50 A/B 383-729 [» ]
    3KAR X-ray 2.30 A 385-729 [» ]
    4ETP X-ray 2.30 A 348-729 [» ]
    ProteinModelPortali P17119.
    SMRi P17119. Positions 343-723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36310. 285 interactions.
    DIPi DIP-75N.
    IntActi P17119. 8 interactions.
    MINTi MINT-435688.
    STRINGi 4932.YPR141C.

    Proteomic databases

    MaxQBi P17119.
    PaxDbi P17119.
    PeptideAtlasi P17119.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR141C ; YPR141C ; YPR141C .
    GeneIDi 856263.
    KEGGi sce:YPR141C.

    Organism-specific databases

    CYGDi YPR141c.
    SGDi S000006345. KAR3.

    Phylogenomic databases

    eggNOGi COG5059.
    GeneTreei ENSGT00550000074610.
    HOGENOMi HOG000116164.
    KOi K10405.
    OMAi CKLESEE.
    OrthoDBi EOG7RRFGF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34276-MONOMER.
    Reactomei REACT_189062. Kinesins.

    Miscellaneous databases

    EvolutionaryTracei P17119.
    NextBioi 981560.
    PROi P17119.

    Gene expression databases

    Genevestigatori P17119.

    Family and domain databases

    Gene3Di 3.40.850.10. 1 hit.
    InterProi IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR24115. PTHR24115. 1 hit.
    Pfami PF00225. Kinesin. 1 hit.
    [Graphical view ]
    PRINTSi PR00380. KINESINHEAVY.
    SMARTi SM00129. KISc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "KAR3, a kinesin-related gene required for yeast nuclear fusion."
      Meluh P.B., Rose M.D.
      Cell 60:1029-1041(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: MY1124.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations."
      Hoyt M.A., He L., Totis L., Saunders W.S.
      Genetics 135:35-44(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTANTS KAR3.
    5. "Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends."
      Endow S.A., Kang S.J., Satterwhite L.L., Rose M.D., Skeen V.P., Salmon E.D.
      EMBO J. 13:2708-2713(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p."
      Manning B.D., Barrett J.G., Wallace J.A., Granok H., Snyder M.
      J. Cell Biol. 144:1219-1233(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CIK1 AND VIK1, SUBCELLULAR LOCATION.
    7. "The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae."
      Shanks R.M.Q., Kamieniecki R.J., Dawson D.S.
      Genetics 159:939-951(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3-A resolution."
      Gulick A.M., Song H., Endow S.A., Rayment I.
      Biochemistry 37:1769-1776(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 383-729.

    Entry informationi

    Entry nameiKAR3_YEAST
    AccessioniPrimary (citable) accession number: P17119
    Secondary accession number(s): D6W4D9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 150 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    KAR3 contains two globular domains separated by an alpha-helical coiled coil. The N-terminal portion of KAR3 contains a microtubule association domain distinct from the kinesin-like C-terminal domain.
    Present with 3250 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3