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Protein

Kinesin-like protein KAR3

Gene

KAR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for yeast nuclear fusion during mating. KAR3 is a bifunctional protein having a kinesin-like motor domain joined to a distinct microtubule binding domain. It may mediate microtubule sliding during nuclear fusion and possibly mitosis. May interact with spindle microtubules to produce an inwardly directed force acting upon the poles. KAR3 function antagonizes CIP8 and KIP1 outward force action. KAR3 motor activity is directed toward the microtubule's minus end.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi474 – 4818ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent microtubule motor activity, minus-end-directed Source: SGD
  3. microtubule binding Source: SGD
  4. protein heterodimerization activity Source: SGD

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. karyogamy involved in conjugation with cellular fusion Source: SGD
  3. meiotic nuclear division Source: SGD
  4. metabolic process Source: GOC
  5. mitotic nuclear division Source: SGD
  6. mitotic sister chromatid cohesion Source: SGD
  7. nuclear migration involved in conjugation with cellular fusion Source: SGD
  8. regulation of mitotic spindle organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Karyogamy

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34276-MONOMER.
ReactomeiREACT_189062. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KAR3
Alternative name(s):
Nuclear fusion protein
Gene namesi
Name:KAR3
Ordered Locus Names:YPR141C
ORF Names:P9659.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR141c.
SGDiS000006345. KAR3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasmic microtubule Source: SGD
  2. kinesin complex Source: InterPro
  3. nucleus Source: UniProtKB-SubCell
  4. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi479 – 4791G → E: Poisons nuclear fusion.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Kinesin-like protein KAR3PRO_0000125391Add
BLAST

Proteomic databases

MaxQBiP17119.
PaxDbiP17119.
PeptideAtlasiP17119.

Expressioni

Inductioni

By alpha factor.

Gene expression databases

GenevestigatoriP17119.

Interactioni

Subunit structurei

Interacts with CIK1 and VIK1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SMC1P329084EBI-9499,EBI-17402
VIK1Q120454EBI-9499,EBI-38784

Protein-protein interaction databases

BioGridi36310. 285 interactions.
DIPiDIP-75N.
IntActiP17119. 8 interactions.
MINTiMINT-435688.
STRINGi4932.YPR141C.

Structurei

Secondary structure

1
729
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi348 – 38437Combined sources
Beta strandi386 – 3938Combined sources
Turni398 – 4003Combined sources
Beta strandi406 – 4105Combined sources
Turni415 – 4184Combined sources
Beta strandi419 – 4268Combined sources
Helixi427 – 4293Combined sources
Beta strandi433 – 44210Combined sources
Helixi448 – 45912Combined sources
Helixi460 – 4645Combined sources
Beta strandi468 – 4736Combined sources
Helixi480 – 4856Combined sources
Turni487 – 4893Combined sources
Helixi491 – 50616Combined sources
Helixi507 – 5093Combined sources
Beta strandi512 – 52312Combined sources
Beta strandi526 – 5294Combined sources
Beta strandi549 – 5524Combined sources
Turni553 – 5564Combined sources
Beta strandi557 – 5604Combined sources
Beta strandi566 – 5705Combined sources
Helixi571 – 5733Combined sources
Helixi574 – 5818Combined sources
Helixi596 – 5983Combined sources
Beta strandi599 – 61012Combined sources
Turni612 – 6143Combined sources
Beta strandi617 – 62610Combined sources
Helixi635 – 6373Combined sources
Helixi640 – 66324Combined sources
Turni668 – 6703Combined sources
Helixi675 – 6773Combined sources
Helixi679 – 68810Combined sources
Beta strandi693 – 7008Combined sources
Helixi704 – 7063Combined sources
Helixi707 – 72014Combined sources
Turni723 – 7253Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F9TX-ray1.50A372-729[»]
1F9UX-ray1.70A383-729[»]
1F9VX-ray1.30A383-729[»]
1F9WX-ray2.50A/B383-729[»]
3KARX-ray2.30A385-729[»]
4ETPX-ray2.30A348-729[»]
ProteinModelPortaliP17119.
SMRiP17119. Positions 343-723.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17119.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini386 – 723338Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 109109GlobularAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili110 – 357248Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. NCD subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00550000074610.
HOGENOMiHOG000116164.
InParanoidiP17119.
KOiK10405.
OMAiCEFIEIY.
OrthoDBiEOG7RRFGF.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLPRTPTK GRSTQHLSTP SPKNDILAMN GHKRRNTTTP PPKHTLLKPQ
60 70 80 90 100
RTDIHRHSLA SQSRISMSPN RELLKNYKGT ANLIYGNQKS NSGVTSFYKE
110 120 130 140 150
NVNELNRTQA ILFEKKATLD LLKDELTETK EKINAVNLKF ETLREEKIKI
160 170 180 190 200
EQQLNLKNNE LISIKEEFLS KKQFMNEGHE IHLKQLAASN KKELKQMENE
210 220 230 240 250
YKTKIEKLKF MKIKQFENER ASLLDKIEEV RNKITMNPST LQEMLNDVEQ
260 270 280 290 300
KHMLEKEEWL TEYQSQWKKD IELNNKHMQE IESIKKEIEN TLKPELAEKK
310 320 330 340 350
KLLTEKRNAY EAIKVKVKEK EEETTRLRDE VALKQKTNLE TLEKIKELEE
360 370 380 390 400
YIKDTELGMK ELNEILIKEE TVRRTLHNEL QELRGNIRVY CRIRPALKNL
410 420 430 440 450
ENSDTSLINV NEFDDNSGVQ SMEVTKIQNT AQVHEFKFDK IFDQQDTNVD
460 470 480 490 500
VFKEVGQLVQ SSLDGYNVCI FAYGQTGSGK TFTMLNPGDG IIPSTISHIF
510 520 530 540 550
NWINKLKTKG WDYKVNCEFI EIYNENIVDL LRSDNNNKED TSIGLKHEIR
560 570 580 590 600
HDQETKTTTI TNVTSCKLES EEMVEIILKK ANKLRSTAST ASNEHSSRSH
610 620 630 640 650
SIFIIHLSGS NAKTGAHSYG TLNLVDLAGS ERINVSQVVG DRLRETQNIN
660 670 680 690 700
KSLSCLGDVI HALGQPDSTK RHIPFRNSKL TYLLQYSLTG DSKTLMFVNI
710 720
SPSSSHINET LNSLRFASKV NSTRLVSRK
Length:729
Mass (Da):84,004
Last modified:August 1, 1990 - v1
Checksum:iBAEF98BC783ABDB9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti378 – 3781N → K in KAR3-894.
Natural varianti462 – 4621S → L in KAR3-891.
Natural varianti521 – 5211E → D in KAR3-893.
Natural varianti550 – 5501R → S in KAR3-899.
Natural varianti558 – 5581T → A in KAR3-8912.
Natural varianti650 – 6501N → K in KAR3-898.
Natural varianti659 – 6591V → L in KAR3-897.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31719 mRNA. Translation: AAA34715.1.
U40829 Genomic DNA. Translation: AAB68281.1.
BK006949 Genomic DNA. Translation: DAA11555.1.
PIRiA34796.
RefSeqiNP_015467.1. NM_001184238.1.

Genome annotation databases

EnsemblFungiiYPR141C; YPR141C; YPR141C.
GeneIDi856263.
KEGGisce:YPR141C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31719 mRNA. Translation: AAA34715.1.
U40829 Genomic DNA. Translation: AAB68281.1.
BK006949 Genomic DNA. Translation: DAA11555.1.
PIRiA34796.
RefSeqiNP_015467.1. NM_001184238.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F9TX-ray1.50A372-729[»]
1F9UX-ray1.70A383-729[»]
1F9VX-ray1.30A383-729[»]
1F9WX-ray2.50A/B383-729[»]
3KARX-ray2.30A385-729[»]
4ETPX-ray2.30A348-729[»]
ProteinModelPortaliP17119.
SMRiP17119. Positions 343-723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36310. 285 interactions.
DIPiDIP-75N.
IntActiP17119. 8 interactions.
MINTiMINT-435688.
STRINGi4932.YPR141C.

Proteomic databases

MaxQBiP17119.
PaxDbiP17119.
PeptideAtlasiP17119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR141C; YPR141C; YPR141C.
GeneIDi856263.
KEGGisce:YPR141C.

Organism-specific databases

CYGDiYPR141c.
SGDiS000006345. KAR3.

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00550000074610.
HOGENOMiHOG000116164.
InParanoidiP17119.
KOiK10405.
OMAiCEFIEIY.
OrthoDBiEOG7RRFGF.

Enzyme and pathway databases

BioCyciYEAST:G3O-34276-MONOMER.
ReactomeiREACT_189062. Kinesins.

Miscellaneous databases

EvolutionaryTraceiP17119.
NextBioi981560.
PROiP17119.

Gene expression databases

GenevestigatoriP17119.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "KAR3, a kinesin-related gene required for yeast nuclear fusion."
    Meluh P.B., Rose M.D.
    Cell 60:1029-1041(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: MY1124.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations."
    Hoyt M.A., He L., Totis L., Saunders W.S.
    Genetics 135:35-44(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTANTS KAR3.
  5. "Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends."
    Endow S.A., Kang S.J., Satterwhite L.L., Rose M.D., Skeen V.P., Salmon E.D.
    EMBO J. 13:2708-2713(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p."
    Manning B.D., Barrett J.G., Wallace J.A., Granok H., Snyder M.
    J. Cell Biol. 144:1219-1233(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CIK1 AND VIK1, SUBCELLULAR LOCATION.
  7. "The Kar3-interacting protein Cik1p plays a critical role in passage through meiosis I in Saccharomyces cerevisiae."
    Shanks R.M.Q., Kamieniecki R.J., Dawson D.S.
    Genetics 159:939-951(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "X-ray crystal structure of the yeast Kar3 motor domain complexed with Mg.ADP to 2.3-A resolution."
    Gulick A.M., Song H., Endow S.A., Rayment I.
    Biochemistry 37:1769-1776(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 383-729.

Entry informationi

Entry nameiKAR3_YEAST
AccessioniPrimary (citable) accession number: P17119
Secondary accession number(s): D6W4D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 4, 2015
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

KAR3 contains two globular domains separated by an alpha-helical coiled coil. The N-terminal portion of KAR3 contains a microtubule association domain distinct from the kinesin-like C-terminal domain.
Present with 3250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.