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Protein

Oxygen-insensitive NADPH nitroreductase

Gene

nfsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.1 Publication

Catalytic activityi

An oxidized nitroaromatic compound + NADPH = a reduced nitroaromatic compound + NADP+.1 Publication

Cofactori

FMN2 PublicationsNote: Binds 1 FMN per monomer.1 Publication

Kineticsi

  1. KM=11 µM for NADPH1 Publication
  2. KM=5.5 µM for nitrofurazone1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei39FMN; via amide nitrogenCombined sources1 Publication1
    Binding sitei67FMNCombined sources1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi11 – 15FMNCombined sources1 Publication5
    Nucleotide bindingi128 – 131FMNCombined sources1 Publication4
    Nucleotide bindingi167 – 169FMNCombined sources1 Publication3

    GO - Molecular functioni

    • chromate reductase activity Source: EcoCyc
    • FMN binding Source: EcoCyc
    • oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11261-MONOMER.
    ECOL316407:JW0835-MONOMER.
    MetaCyc:EG11261-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oxygen-insensitive NADPH nitroreductaseCurated (EC:1.-.-.-1 Publication)
    Alternative name(s):
    Modulator of drug activity A
    Gene namesi
    Name:nfsA
    Synonyms:mda18, mdaA1 Publication, ybjB
    Ordered Locus Names:b0851, JW0835
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11261. nfsA.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi203R → A: Strong decrease in activity. 1 Publication1
    Mutagenesisi208R → A: No change in activity. 1 Publication1

    Chemistry databases

    DrugBankiDB00698. Nitrofurantoin.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002055101 – 240Oxygen-insensitive NADPH nitroreductaseAdd BLAST240

    Proteomic databases

    EPDiP17117.
    PaxDbiP17117.
    PRIDEiP17117.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    holAP286303EBI-1120624,EBI-549153

    Protein-protein interaction databases

    BioGridi4259991. 10 interactors.
    DIPiDIP-10334N.
    IntActiP17117. 5 interactors.
    STRINGi511145.b0851.

    Structurei

    Secondary structure

    1240
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 9Combined sources7
    Helixi24 – 35Combined sources12
    Helixi40 – 42Combined sources3
    Beta strandi46 – 50Combined sources5
    Helixi54 – 63Combined sources10
    Helixi69 – 72Combined sources4
    Beta strandi73 – 82Combined sources10
    Helixi84 – 89Combined sources6
    Helixi98 – 121Combined sources24
    Beta strandi125 – 129Combined sources5
    Helixi130 – 135Combined sources6
    Helixi136 – 142Combined sources7
    Beta strandi149 – 158Combined sources10
    Helixi172 – 175Combined sources4
    Beta strandi176 – 180Combined sources5
    Helixi186 – 201Combined sources16
    Helixi212 – 220Combined sources9
    Helixi228 – 234Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1F5VX-ray1.70A/B1-240[»]
    ProteinModelPortaliP17117.
    SMRiP17117.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17117.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin oxidoreductase frp family.Curated

    Phylogenomic databases

    eggNOGiENOG4108DBC. Bacteria.
    COG0778. LUCA.
    HOGENOMiHOG000272869.
    InParanoidiP17117.
    KOiK10678.
    OMAiAYYKERS.
    PhylomeDBiP17117.

    Family and domain databases

    CDDicd02146. NfsA_FRP. 1 hit.
    Gene3Di3.40.109.10. 1 hit.
    InterProiIPR016446. Flavin_OxRdtase_Frp.
    IPR029479. Nitroreductase.
    IPR000415. Nitroreductase-like.
    [Graphical view]
    PfamiPF00881. Nitroreductase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005426. Frp. 1 hit.
    SUPFAMiSSF55469. SSF55469. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P17117-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTPTIELICG HRSIRHFTDE PISEAQREAI INSARATSSS SFLQCSSIIR
    60 70 80 90 100
    ITDKALREEL VTLTGGQKHV AQAAEFWVFC ADFNRHLQIC PDAQLGLAEQ
    110 120 130 140 150
    LLLGVVDTAM MAQNALIAAE SLGLGGVYIG GLRNNIEAVT KLLKLPQHVL
    160 170 180 190 200
    PLFGLCLGWP ADNPDLKPRL PASILVHENS YQPLDKGALA QYDEQLAEYY
    210 220 230 240
    LTRGSNNRRD TWSDHIRRTI IKESRPFILD YLHKQGWATR
    Length:240
    Mass (Da):26,801
    Last modified:February 1, 1995 - v2
    Checksum:i3B389FCDB86DED1D
    GO

    Sequence cautioni

    The sequence CAA33867 differs from that shown. Reason: Frameshift at positions 155, 161 and 187.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18655 Genomic DNA. Translation: AAC43450.1.
    D38308 Genomic DNA. Translation: BAA07425.1.
    U00096 Genomic DNA. Translation: AAC73938.1.
    AP009048 Genomic DNA. Translation: BAA35562.1.
    X15859 Genomic DNA. Translation: CAA33867.1. Frameshift.
    PIRiI80318.
    RefSeqiNP_415372.1. NC_000913.3.
    WP_000189159.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73938; AAC73938; b0851.
    BAA35562; BAA35562; BAA35562.
    GeneIDi945483.
    KEGGiecj:JW0835.
    eco:b0851.
    PATRICi32116907. VBIEscCol129921_0879.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18655 Genomic DNA. Translation: AAC43450.1.
    D38308 Genomic DNA. Translation: BAA07425.1.
    U00096 Genomic DNA. Translation: AAC73938.1.
    AP009048 Genomic DNA. Translation: BAA35562.1.
    X15859 Genomic DNA. Translation: CAA33867.1. Frameshift.
    PIRiI80318.
    RefSeqiNP_415372.1. NC_000913.3.
    WP_000189159.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1F5VX-ray1.70A/B1-240[»]
    ProteinModelPortaliP17117.
    SMRiP17117.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259991. 10 interactors.
    DIPiDIP-10334N.
    IntActiP17117. 5 interactors.
    STRINGi511145.b0851.

    Chemistry databases

    DrugBankiDB00698. Nitrofurantoin.

    Proteomic databases

    EPDiP17117.
    PaxDbiP17117.
    PRIDEiP17117.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73938; AAC73938; b0851.
    BAA35562; BAA35562; BAA35562.
    GeneIDi945483.
    KEGGiecj:JW0835.
    eco:b0851.
    PATRICi32116907. VBIEscCol129921_0879.

    Organism-specific databases

    EchoBASEiEB1241.
    EcoGeneiEG11261. nfsA.

    Phylogenomic databases

    eggNOGiENOG4108DBC. Bacteria.
    COG0778. LUCA.
    HOGENOMiHOG000272869.
    InParanoidiP17117.
    KOiK10678.
    OMAiAYYKERS.
    PhylomeDBiP17117.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11261-MONOMER.
    ECOL316407:JW0835-MONOMER.
    MetaCyc:EG11261-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP17117.
    PROiP17117.

    Family and domain databases

    CDDicd02146. NfsA_FRP. 1 hit.
    Gene3Di3.40.109.10. 1 hit.
    InterProiIPR016446. Flavin_OxRdtase_Frp.
    IPR029479. Nitroreductase.
    IPR000415. Nitroreductase-like.
    [Graphical view]
    PfamiPF00881. Nitroreductase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005426. Frp. 1 hit.
    SUPFAMiSSF55469. SSF55469. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNFSA_ECOLI
    AccessioniPrimary (citable) accession number: P17117
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: February 1, 1995
    Last modified: November 2, 2016
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.