Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Oxygen-insensitive NADPH nitroreductase

Gene

nfsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.1 Publication

Catalytic activityi

An oxidized nitroaromatic compound + NADPH = a reduced nitroaromatic compound + NADP+.1 Publication

Cofactori

FMN2 PublicationsNote: Binds 1 FMN per monomer.1 Publication

Kineticsi

  1. KM=11 µM for NADPH1 Publication
  2. KM=5.5 µM for nitrofurazone1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei39FMN; via amide nitrogenCombined sources1 Publication1
    Binding sitei67FMNCombined sources1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi11 – 15FMNCombined sources1 Publication5
    Nucleotide bindingi128 – 131FMNCombined sources1 Publication4
    Nucleotide bindingi167 – 169FMNCombined sources1 Publication3

    GO - Molecular functioni

    • chromate reductase activity Source: EcoCyc
    • FMN binding Source: EcoCyc
    • oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor Source: EcoCyc

    Keywordsi

    Molecular functionOxidoreductase
    LigandFlavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11261-MONOMER
    MetaCyc:EG11261-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oxygen-insensitive NADPH nitroreductaseCurated (EC:1.-.-.-1 Publication)
    Alternative name(s):
    Modulator of drug activity A
    Gene namesi
    Name:nfsA
    Synonyms:mda18, mdaA1 Publication, ybjB
    Ordered Locus Names:b0851, JW0835
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11261 nfsA

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi203R → A: Strong decrease in activity. 1 Publication1
    Mutagenesisi208R → A: No change in activity. 1 Publication1

    Chemistry databases

    DrugBankiDB00698 Nitrofurantoin
    DB03247 Riboflavin Monophosphate

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002055101 – 240Oxygen-insensitive NADPH nitroreductaseAdd BLAST240

    Proteomic databases

    EPDiP17117
    PaxDbiP17117
    PRIDEiP17117

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    holAP286303EBI-1120624,EBI-549153

    Protein-protein interaction databases

    BioGridi4259991, 28 interactors
    DIPiDIP-10334N
    IntActiP17117, 5 interactors
    STRINGi316385.ECDH10B_0921

    Structurei

    Secondary structure

    1240
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 9Combined sources7
    Helixi24 – 35Combined sources12
    Helixi40 – 42Combined sources3
    Beta strandi46 – 50Combined sources5
    Helixi54 – 63Combined sources10
    Helixi69 – 72Combined sources4
    Beta strandi73 – 82Combined sources10
    Helixi84 – 89Combined sources6
    Helixi98 – 121Combined sources24
    Beta strandi125 – 129Combined sources5
    Helixi130 – 135Combined sources6
    Helixi136 – 142Combined sources7
    Beta strandi149 – 158Combined sources10
    Helixi172 – 175Combined sources4
    Beta strandi176 – 180Combined sources5
    Helixi186 – 201Combined sources16
    Helixi212 – 220Combined sources9
    Helixi228 – 234Combined sources7

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1F5VX-ray1.70A/B1-240[»]
    ProteinModelPortaliP17117
    SMRiP17117
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17117

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin oxidoreductase frp family.Curated

    Phylogenomic databases

    eggNOGiENOG4108DBC Bacteria
    COG0778 LUCA
    HOGENOMiHOG000272869
    InParanoidiP17117
    KOiK10678
    OMAiGMCLGYP
    PhylomeDBiP17117

    Family and domain databases

    CDDicd02146 NfsA_FRP, 1 hit
    Gene3Di3.40.109.10, 1 hit
    InterProiView protein in InterPro
    IPR016446 Flavin_OxRdtase_Frp
    IPR029479 Nitroreductase
    IPR000415 Nitroreductase-like
    PANTHERiPTHR43425 PTHR43425, 1 hit
    PfamiView protein in Pfam
    PF00881 Nitroreductase, 1 hit
    PIRSFiPIRSF005426 Frp, 1 hit
    SUPFAMiSSF55469 SSF55469, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P17117-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTPTIELICG HRSIRHFTDE PISEAQREAI INSARATSSS SFLQCSSIIR
    60 70 80 90 100
    ITDKALREEL VTLTGGQKHV AQAAEFWVFC ADFNRHLQIC PDAQLGLAEQ
    110 120 130 140 150
    LLLGVVDTAM MAQNALIAAE SLGLGGVYIG GLRNNIEAVT KLLKLPQHVL
    160 170 180 190 200
    PLFGLCLGWP ADNPDLKPRL PASILVHENS YQPLDKGALA QYDEQLAEYY
    210 220 230 240
    LTRGSNNRRD TWSDHIRRTI IKESRPFILD YLHKQGWATR
    Length:240
    Mass (Da):26,801
    Last modified:February 1, 1995 - v2
    Checksum:i3B389FCDB86DED1D
    GO

    Sequence cautioni

    The sequence CAA33867 differs from that shown. Reason: Frameshift at positions 155, 161 and 187.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18655 Genomic DNA Translation: AAC43450.1
    D38308 Genomic DNA Translation: BAA07425.1
    U00096 Genomic DNA Translation: AAC73938.1
    AP009048 Genomic DNA Translation: BAA35562.1
    X15859 Genomic DNA Translation: CAA33867.1 Frameshift.
    PIRiI80318
    RefSeqiNP_415372.1, NC_000913.3
    WP_000189159.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73938; AAC73938; b0851
    BAA35562; BAA35562; BAA35562
    GeneIDi945483
    KEGGiecj:JW0835
    eco:b0851
    PATRICifig|1411691.4.peg.1427

    Entry informationi

    Entry nameiNFSA_ECOLI
    AccessioniPrimary (citable) accession number: P17117
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: February 1, 1995
    Last modified: March 28, 2018
    This is version 143 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Cookie policy

    We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health