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Protein

Arabinose 5-phosphate isomerase GutQ

Gene

gutQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P). It appears that the physiological function of G-API may be to synthesize the regulatory molecule A5P, which in turn participates in the induction of the gut operon through an unknown mechanism. It is also able of sustaining the biosynthetic pathway of 3-deoxy-D-manno-octulosonate (KDO), a unique 8-carbon sugar component of lipopolysaccharides (LPSs).1 Publication

Catalytic activityi

D-arabinose 5-phosphate = D-ribulose 5-phosphate.

Kineticsi

  1. KM=0.64 mM for Ru5P (at pH 8.25 and at 37 degrees Celsius)1 Publication
  2. KM=1.2 mM for A5P (at pH 8.25 and at 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 8.25.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei52 – 521Catalytically relevantBy similarity
    Metal bindingi75 – 751ZincBy similarity
    Binding sitei75 – 751SubstrateBy similarity
    Binding sitei81 – 811SubstrateBy similarity
    Sitei104 – 1041Catalytically relevantBy similarity
    Sitei145 – 1451Catalytically relevantBy similarity
    Sitei186 – 1861Catalytically relevantBy similarity
    Binding sitei215 – 2151SubstrateBy similarity
    Binding sitei267 – 2671SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi49 – 546ATPSequence analysis

    GO - Molecular functioni

    GO - Biological processi

    • keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Source: UniProtKB
    • single-species biofilm formation Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10973-MONOMER.
    ECOL316407:JW5431-MONOMER.
    MetaCyc:EG10973-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arabinose 5-phosphate isomerase GutQ (EC:5.3.1.13)
    Short name:
    API
    Short name:
    G-API
    Alternative name(s):
    Phosphosugar aldol-ketol isomerase
    Gene namesi
    Name:gutQ
    Synonyms:srlQ
    Ordered Locus Names:b2708, JW5431
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10973. gutQ.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemoved1 Publication
    Chaini2 – 321320Arabinose 5-phosphate isomerase GutQPRO_0000136572Add
    BLAST

    Proteomic databases

    PaxDbiP17115.
    PRIDEiP17115.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi4263273. 290 interactions.
    IntActiP17115. 2 interactions.
    STRINGi511145.b2708.

    Structurei

    3D structure databases

    ProteinModelPortaliP17115.
    SMRiP17115. Positions 8-176, 192-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 177144SISPROSITE-ProRule annotationAdd
    BLAST
    Domaini203 – 26159CBS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini269 – 32153CBS 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni68 – 692Substrate bindingBy similarity
    Regioni107 – 11610Substrate bindingBy similarity
    Regioni141 – 1433Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the SIS family. GutQ/KpsF subfamily.Curated
    Contains 2 CBS domains.PROSITE-ProRule annotation
    Contains 1 SIS domain.PROSITE-ProRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    eggNOGiENOG4105C2X. Bacteria.
    COG0517. LUCA.
    COG0794. LUCA.
    HOGENOMiHOG000264729.
    InParanoidiP17115.
    KOiK02467.
    OMAiAVCDEAN.
    PhylomeDBiP17115.

    Family and domain databases

    InterProiIPR000644. CBS_dom.
    IPR004800. KdsD/KpsF-type.
    IPR001347. SIS.
    [Graphical view]
    PfamiPF00571. CBS. 1 hit.
    PF01380. SIS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004692. KdsD_KpsF. 1 hit.
    TIGRFAMsiTIGR00393. kpsF. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS51464. SIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P17115-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSEALLNAGR QTLMLELQEA SRLPERLGDD FVRAANIILH CEGKVVVSGI
    60 70 80 90 100
    GKSGHIGKKI AATLASTGTP AFFVHPAEAL HGDLGMIESR DVMLFISYSG
    110 120 130 140 150
    GAKELDLIIP RLEDKSIALL AMTGKPTSPL GLAAKAVLDI SVEREACPMH
    160 170 180 190 200
    LAPTSSTVNT LMMGDALAMA VMQARGFNEE DFARSHPAGA LGARLLNKVH
    210 220 230 240 250
    HLMRRDDAIP QVALTASVMD AMLELSRTGL GLVAVCDAQQ QVQGVFTDGD
    260 270 280 290 300
    LRRWLVGGGA LTTPVNEAMT VGGTTLQSQS RAIDAKEILM KRKITAAPVV
    310 320
    DENGKLTGAI NLQDFYQAGI I
    Length:321
    Mass (Da):34,031
    Last modified:July 11, 2001 - v3
    Checksum:i85C31DFBD92F7B7C
    GO

    Sequence cautioni

    The sequence AAA69217 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence CAA35745 differs from that shown. Reason: Frameshift at position 214. Curated
    The sequence CAA35745 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U29579 Genomic DNA. Translation: AAA69217.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC75750.2.
    AP009048 Genomic DNA. Translation: BAE76785.1.
    X51361 Genomic DNA. Translation: CAA35745.1. Sequence problems.
    RefSeqiNP_417188.4. NC_000913.3.
    WP_001287420.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75750; AAC75750; b2708.
    BAE76785; BAE76785; BAE76785.
    GeneIDi947587.
    KEGGiecj:JW5431.
    eco:b2708.
    PATRICi32120814. VBIEscCol129921_2799.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U29579 Genomic DNA. Translation: AAA69217.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC75750.2.
    AP009048 Genomic DNA. Translation: BAE76785.1.
    X51361 Genomic DNA. Translation: CAA35745.1. Sequence problems.
    RefSeqiNP_417188.4. NC_000913.3.
    WP_001287420.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP17115.
    SMRiP17115. Positions 8-176, 192-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4263273. 290 interactions.
    IntActiP17115. 2 interactions.
    STRINGi511145.b2708.

    Proteomic databases

    PaxDbiP17115.
    PRIDEiP17115.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75750; AAC75750; b2708.
    BAE76785; BAE76785; BAE76785.
    GeneIDi947587.
    KEGGiecj:JW5431.
    eco:b2708.
    PATRICi32120814. VBIEscCol129921_2799.

    Organism-specific databases

    EchoBASEiEB0966.
    EcoGeneiEG10973. gutQ.

    Phylogenomic databases

    eggNOGiENOG4105C2X. Bacteria.
    COG0517. LUCA.
    COG0794. LUCA.
    HOGENOMiHOG000264729.
    InParanoidiP17115.
    KOiK02467.
    OMAiAVCDEAN.
    PhylomeDBiP17115.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10973-MONOMER.
    ECOL316407:JW5431-MONOMER.
    MetaCyc:EG10973-MONOMER.

    Miscellaneous databases

    PROiP17115.

    Family and domain databases

    InterProiIPR000644. CBS_dom.
    IPR004800. KdsD/KpsF-type.
    IPR001347. SIS.
    [Graphical view]
    PfamiPF00571. CBS. 1 hit.
    PF01380. SIS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004692. KdsD_KpsF. 1 hit.
    TIGRFAMsiTIGR00393. kpsF. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS51464. SIS. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGUTQ_ECOLI
    AccessioniPrimary (citable) accession number: P17115
    Secondary accession number(s): Q2MAC1, Q46874
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: July 11, 2001
    Last modified: September 7, 2016
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.