Reviewed, UniProtKB/Swiss-Prot P17089 (URED_PROMH)

Names and origin

Protein names

Recommended name:
    Urease accessory protein ureD

Gene names

Name:	ureD
Ordered Locus Names:	PMI3682

Organism

Proteus mirabilis (strain HI4320) [Complete proteome] [HAMAP]

Taxonomic identifier

529507 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity.
Subunit structure	UreD, ureF and ureG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of ureC. The ureE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	By urea. Ref.4
Sequence similarities	Belongs to the ureD family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	Urease accessory protein ureD HAMAP MF_01384		PRO_0000067613
Experimental info
	Mutagenesis	270	1	R → RRRRI: Reduces activity 14-fold. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P17089-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 5B52FAAFD3573A9E	FASTA	274	31,009
	10 20 30 40 50 60 MPDFSEKGWL ADIALRYELK RGKTCLTEKR HLGPLMVQRP FYPEQGVAHT YLLHPPGGVV 70 80 90 100 110 120 GGDTLSININ VQPYAHALLT TPGATKFYRS AGGTASQTQT LTVAQEGFLE WLPQENIFFP 130 140 150 160 170 180 DAQVCLTTHI HLASSAKFIG WEMQCFGRPV LNEWFETGKV KGRLNFYVDE RLILTESMRV 190 200 210 220 230 240 EGLQKQAAAM REFPMFGSLY IYPATDALKE IIQHHLEKVN PLVEYGLTDV DGILVLRVLG 250 260 270 TQTEPMMACF AQVWQIVRQH WLGYCPEPPR IWAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Proteus mirabilis urease: nucleotide sequence determination and comparison with jack bean urease." Jones B.D., Mobley H.L.T. J. Bacteriol. 171:6414-6422(1989) [PubMed: 2687233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility." Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S., Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Walker D., Whithead S. , Thomson N.R., Rather P.N., Parkhill J., Mobley H.L.T. J. Bacteriol. 190:4027-4037(2008) [PubMed: 18375554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection." Jones B.D., Lockatell C.V., Johnson D.E., Warren J.W., Mobley H.L.T. Infect. Immun. 58:1120-1123(1990) [PubMed: 2180821] [Abstract] Cited for: UREASE AS A VIRULENCE FACTOR.
[4]	"Proteus mirabilis urease: transcriptional regulation by UreR." Nicholson E.B., Concaugh E.A., Foxall P.A., Island M.D., Mobley H.L.T. J. Bacteriol. 175:465-473(1993) [PubMed: 7678244] [Abstract] Cited for: INDUCTION.
[5]	"Proteus mirabilis urease: operon fusion and linker insertion analysis of ure gene organization, regulation, and function." Island M.D., Mobley H.L.T. J. Bacteriol. 177:5653-5660(1995) [PubMed: 7559355] [Abstract] Cited for: PROBABLE OPERON STRUCTURE, MUTAGENESIS OF ARG-270.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M31834 Genomic DNA. Translation: AAA25666.1. AM942759 Genomic DNA. Translation: CAR47180.1.
PIR	A43719.
RefSeq	YP_002153357.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6801576.
GenomeReviews	Gene locus PMI3682 in contig AM942759_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	P17089. PQSSIFF.
Family and domain databases
HAMAP	MF_01384. [Tree]
InterPro	IPR002669. UreD. [Graphical view]
Pfam	PF01774. UreD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URED_PROMH

Accession

Primary (citable) accession number: P17089
Secondary accession number(s): B4EXN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)