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P17076 (RL8A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60S ribosomal protein L8-A
Alternative name(s):
L4
L4-2
L7a-1
Maintenance of killer protein 7
RP6
YL5
Gene names
Name:RPL8A
Synonyms:MAK7, RPL4A
Ordered Locus Names:YHL033C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.8

Subcellular location

Cytoplasm Ref.10.

Miscellaneous

Present with 238000 molecules/cell in log phase SD medium.

There are 2 genes for L8 in yeast.

Sequence similarities

Belongs to the ribosomal protein L7Ae family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6 Ref.9
Chain2 – 25625560S ribosomal protein L8-A
PRO_0000136760

Experimental info

Sequence conflict1131A → R in CAA35073. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P17076 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: E3C8537D8683C905

FASTA25628,125
        10         20         30         40         50         60 
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR 

        70         80         90        100        110        120 
QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE TAAEKKERLT KEAAAVAEGK 

       130        140        150        160        170        180 
SKQDASPKPY AVKYGLNHVV ALIENKKAKL VLIANDVDPI ELVVFLPALC KKMGVPYAIV 

       190        200        210        220        230        240 
KGKARLGTLV NQKTSAVAAL TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN 

       250 
KAQAKMDKRA KNSDSA 

« Hide

References

« Hide 'large scale' references
[1]"The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a."
Yon J., Giallongo A., Fried M.
Mol. Gen. Genet. 227:72-80(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Ribosomal protein L4 of Saccharomyces cerevisiae: the gene and its protein."
Arevalo S.G., Warner J.R.
Nucleic Acids Res. 18:1447-1450(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204626 / S288c / A364A.
[3]"Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast."
Wickner R.B., Leibowitz M.J.
J. Mol. Biol. 105:427-443(1976) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-20.
[7]"Yeast virus propagation depends critically on free 60S ribosomal subunit concentration."
Ohtake Y., Wickner R.B.
Mol. Cell. Biol. 15:2772-2781(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION AS MAK7.
[8]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[9]"The action of N-terminal acetyltransferases on yeast ribosomal proteins."
Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[10]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
[13]"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
[14]"Crystal structure of the eukaryotic ribosome."
Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
[15]"The structure of the eukaryotic ribosome at 3.0 A resolution."
Ben-Shem A., Garreau de Loubresse N., Melnikov S., Jenner L., Yusupova G., Yusupov M.
Science 334:1524-1529(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17204 Genomic DNA. Translation: CAA35073.1.
X56836 Genomic DNA. Translation: CAA40166.1.
U17361 Genomic DNA. Translation: AAA64574.1.
U11583 Genomic DNA. Translation: AAB65045.1.
BK006934 Genomic DNA. Translation: DAA06652.1.
PIRR5BY7A. S16811.
RefSeqNP_011830.1. NM_001179113.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-G102-220[»]
1S1Ielectron microscopy11.70G102-220[»]
3IZSelectron microscopy-H1-256[»]
3O58X-ray4.00H1-256[»]
3O5HX-ray4.00H1-256[»]
3U5EX-ray3.00G1-256[»]
3U5IX-ray3.00G1-256[»]
4B6Aelectron microscopy8.10G1-256[»]
4BYNelectron microscopy4.30G2-256[»]
4BYUelectron microscopy6.60G2-256[»]
ProteinModelPortalP17076.
SMRP17076. Positions 24-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36389. 186 interactions.
DIPDIP-5102N.
IntActP17076. 30 interactions.
MINTMINT-483885.
STRING4932.YHL033C.

Proteomic databases

PeptideAtlasP17076.
PRIDEP17076.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHL033C; YHL033C; YHL033C.
GeneID856352.
KEGGsce:YHL033C.

Organism-specific databases

SGDS000001025. RPL8A.

Phylogenomic databases

GeneTreeENSGT00390000004753.
HOGENOMHOG000216644.
KOK02936.
OMATHRNTHY.
OrthoDBEOG75QRG2.

Enzyme and pathway databases

BioCycYEAST:G3O-31052-MONOMER.

Gene expression databases

GenevestigatorP17076.

Family and domain databases

InterProIPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]
PANTHERPTHR23105:SF1. PTHR23105:SF1. 1 hit.
PfamPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSPR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.
PROSITEPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP17076.
NextBio981793.

Entry information

Entry nameRL8A_YEAST
AccessionPrimary (citable) accession number: P17076
Secondary accession number(s): D3DKT5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 139 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references