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P17076

- RL8A_YEAST

UniProt

P17076 - RL8A_YEAST

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Protein

60S ribosomal protein L8-A

Gene

RPL8A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. maturation of LSU-rRNA Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-31052-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L8-A
Alternative name(s):
L4
L4-2
L7a-1
Maintenance of killer protein 7
RP6
YL5
Gene namesi
Name:RPL8A
Synonyms:MAK7, RPL4A
Ordered Locus Names:YHL033C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

SGDiS000001025. RPL8A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 25625560S ribosomal protein L8-APRO_0000136760Add
BLAST

Proteomic databases

MaxQBiP17076.
PeptideAtlasiP17076.
PRIDEiP17076.

Expressioni

Gene expression databases

GenevestigatoriP17076.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi36389. 189 interactions.
DIPiDIP-5102N.
IntActiP17076. 30 interactions.
MINTiMINT-483885.
STRINGi4932.YHL033C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-G102-220[»]
1S1Ielectron microscopy11.70G102-220[»]
1VW8electron microscopy6.10H1-256[»]
1VWUelectron microscopy6.10H1-256[»]
1VXVelectron microscopy6.20H1-256[»]
1VXYelectron microscopy6.30H1-256[»]
3IZSelectron microscopy-H1-256[»]
3J65electron microscopy8.70H1-256[»]
3O58X-ray4.00H1-256[»]
3O5HX-ray4.00H1-256[»]
3U5EX-ray3.00G1-256[»]
3U5IX-ray3.00G1-256[»]
4B6Aelectron microscopy8.10G1-256[»]
4BYNelectron microscopy4.30G2-256[»]
4BYUelectron microscopy6.60G2-256[»]
4CUWelectron microscopy3.70G1-256[»]
ProteinModelPortaliP17076.
SMRiP17076. Positions 24-256.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP17076.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L7Ae family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004753.
HOGENOMiHOG000216644.
InParanoidiP17076.
KOiK02936.
OMAiPSLCKKM.
OrthoDBiEOG75QRG2.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
InterProiIPR029064. L30e-like.
IPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]
PANTHERiPTHR23105:SF1. PTHR23105:SF1. 1 hit.
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.
SUPFAMiSSF55315. SSF55315. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17076-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV
60 70 80 90 100
KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE
110 120 130 140 150
TAAEKKERLT KEAAAVAEGK SKQDASPKPY AVKYGLNHVV ALIENKKAKL
160 170 180 190 200
VLIANDVDPI ELVVFLPALC KKMGVPYAIV KGKARLGTLV NQKTSAVAAL
210 220 230 240 250
TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN KAQAKMDKRA

KNSDSA
Length:256
Mass (Da):28,125
Last modified:January 23, 2007 - v4
Checksum:iE3C8537D8683C905
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131A → R in CAA35073. (PubMed:2183194)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17204 Genomic DNA. Translation: CAA35073.1.
X56836 Genomic DNA. Translation: CAA40166.1.
U17361 Genomic DNA. Translation: AAA64574.1.
U11583 Genomic DNA. Translation: AAB65045.1.
BK006934 Genomic DNA. Translation: DAA06652.1.
PIRiS16811. R5BY7A.
RefSeqiNP_011830.1. NM_001179113.1.

Genome annotation databases

EnsemblFungiiYHL033C; YHL033C; YHL033C.
GeneIDi856352.
KEGGisce:YHL033C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17204 Genomic DNA. Translation: CAA35073.1 .
X56836 Genomic DNA. Translation: CAA40166.1 .
U17361 Genomic DNA. Translation: AAA64574.1 .
U11583 Genomic DNA. Translation: AAB65045.1 .
BK006934 Genomic DNA. Translation: DAA06652.1 .
PIRi S16811. R5BY7A.
RefSeqi NP_011830.1. NM_001179113.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1K5Y model - G 102-220 [» ]
1S1I electron microscopy 11.70 G 102-220 [» ]
1VW8 electron microscopy 6.10 H 1-256 [» ]
1VWU electron microscopy 6.10 H 1-256 [» ]
1VXV electron microscopy 6.20 H 1-256 [» ]
1VXY electron microscopy 6.30 H 1-256 [» ]
3IZS electron microscopy - H 1-256 [» ]
3J65 electron microscopy 8.70 H 1-256 [» ]
3O58 X-ray 4.00 H 1-256 [» ]
3O5H X-ray 4.00 H 1-256 [» ]
3U5E X-ray 3.00 G 1-256 [» ]
3U5I X-ray 3.00 G 1-256 [» ]
4B6A electron microscopy 8.10 G 1-256 [» ]
4BYN electron microscopy 4.30 G 2-256 [» ]
4BYU electron microscopy 6.60 G 2-256 [» ]
4CUW electron microscopy 3.70 G 1-256 [» ]
ProteinModelPortali P17076.
SMRi P17076. Positions 24-256.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36389. 189 interactions.
DIPi DIP-5102N.
IntActi P17076. 30 interactions.
MINTi MINT-483885.
STRINGi 4932.YHL033C.

Proteomic databases

MaxQBi P17076.
PeptideAtlasi P17076.
PRIDEi P17076.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHL033C ; YHL033C ; YHL033C .
GeneIDi 856352.
KEGGi sce:YHL033C.

Organism-specific databases

SGDi S000001025. RPL8A.

Phylogenomic databases

GeneTreei ENSGT00390000004753.
HOGENOMi HOG000216644.
InParanoidi P17076.
KOi K02936.
OMAi PSLCKKM.
OrthoDBi EOG75QRG2.

Enzyme and pathway databases

BioCyci YEAST:G3O-31052-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

EvolutionaryTracei P17076.
NextBioi 981793.

Gene expression databases

Genevestigatori P17076.

Family and domain databases

Gene3Di 3.30.1330.30. 1 hit.
InterProi IPR029064. L30e-like.
IPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view ]
PANTHERi PTHR23105:SF1. PTHR23105:SF1. 1 hit.
Pfami PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view ]
PRINTSi PR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.
SUPFAMi SSF55315. SSF55315. 1 hit.
PROSITEi PS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a."
    Yon J., Giallongo A., Fried M.
    Mol. Gen. Genet. 227:72-80(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Ribosomal protein L4 of Saccharomyces cerevisiae: the gene and its protein."
    Arevalo S.G., Warner J.R.
    Nucleic Acids Res. 18:1447-1450(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204626 / S288c / A364A.
  3. "Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast."
    Wickner R.B., Leibowitz M.J.
    J. Mol. Biol. 105:427-443(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20.
  7. "Yeast virus propagation depends critically on free 60S ribosomal subunit concentration."
    Ohtake Y., Wickner R.B.
    Mol. Cell. Biol. 15:2772-2781(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS MAK7.
  8. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  9. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  12. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
  13. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
  14. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  15. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL8A_YEAST
AccessioniPrimary (citable) accession number: P17076
Secondary accession number(s): D3DKT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 145 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 238000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L8 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3