60S ribosomal protein L8-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P17076 (RL8A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 132. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L8-A

Alternative name(s):
L4
L4-2
L7a-1
Maintenance of killer protein 7
RP6
YL5

Gene names

Name:	RPL8A
Synonyms:	MAK7, RPL4A
Ordered Locus Names:	YHL033C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.8
Subcellular location	Cytoplasm Ref.10.
Miscellaneous	Present with 238000 molecules/cell in log phase SD medium. There are 2 genes for L8 in yeast.
Sequence similarities	Belongs to the ribosomal protein L7Ae family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.8. Source: SGD ribosome biogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.8. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.6 Ref.9

Chain

2 – 256

255

60S ribosomal protein L8-A

PRO_0000136760

Amino acid modifications

Modified residue

126

Phosphoserine Ref.12 Ref.14

Modified residue

216

Phosphoserine Ref.13 Ref.14

Experimental info

Sequence conflict

113

A → R in CAA35073. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P17076 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: E3C8537D8683C905
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FASTA

256

28,125

        10         20         30         40         50         60 
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR 

        70         80         90        100        110        120 
QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE TAAEKKERLT KEAAAVAEGK 

       130        140        150        160        170        180 
SKQDASPKPY AVKYGLNHVV ALIENKKAKL VLIANDVDPI ELVVFLPALC KKMGVPYAIV 

       190        200        210        220        230        240 
KGKARLGTLV NQKTSAVAAL TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN 

       250 
KAQAKMDKRA KNSDSA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a." Yon J., Giallongo A., Fried M. Mol. Gen. Genet. 227:72-80(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Ribosomal protein L4 of Saccharomyces cerevisiae: the gene and its protein." Arevalo S.G., Warner J.R. Nucleic Acids Res. 18:1447-1450(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204626 / S288c / A364A.
[3]	"Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast." Wickner R.B., Leibowitz M.J. J. Mol. Biol. 105:427-443(1976) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20.
[7]	"Yeast virus propagation depends critically on free 60S ribosomal subunit concentration." Ohtake Y., Wickner R.B. Mol. Cell. Biol. 15:2772-2781(1995) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS MAK7.
[8]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[9]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126, MASS SPECTROMETRY.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126 AND SER-216, MASS SPECTROMETRY.
[15]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
[16]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X17204 Genomic DNA. Translation: CAA35073.1.
X56836 Genomic DNA. Translation: CAA40166.1.
U17361 Genomic DNA. Translation: AAA64574.1.
U11583 Genomic DNA. Translation: AAB65045.1.
BK006934 Genomic DNA. Translation: DAA06652.1.

PIR

R5BY7A. S16811.

RefSeq

NP_011830.1. NM_001179113.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	G	102-220	[»]
1S1I	electron microscopy	11.70	G	102-220	[»]
3IZS	electron microscopy	-	H	1-256	[»]
3O58	X-ray	4.00	H	1-256	[»]
3O5H	X-ray	4.00	H	1-256	[»]
3U5E	X-ray	3.00	G	1-256	[»]
3U5I	X-ray	3.00	G	1-256	[»]
4B6A	electron microscopy	8.10	G	1-256	[»]

ProteinModelPortal

P17076.

SMR

P17076. Positions 24-256.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5102N.

IntAct

P17076. 25 interactions.

MINT

MINT-483885.

STRING

4932.YHL033C.

Proteomic databases

PeptideAtlas

P17076.

PRIDE

P17076.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YHL033C; YHL033C; YHL033C.

GeneID

856352.

KEGG

sce:YHL033C.

Organism-specific databases

SGD

S000001025. RPL8A.

Phylogenomic databases

GeneTree

ENSGT00390000004753.

HOGENOM

HOG000216644.

K02936.

OMA

YRPETKV.

OrthoDB

EOG4KWP2Z.

Gene expression databases

Genevestigator

P17076.

GermOnline

YHL033C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001921. Ribosomal_L7A/L8.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]

PANTHER

PTHR23105:SF1. PTHR23105:SF1. 1 hit.

Pfam

PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]

PRINTS

PR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.

PROSITE

PS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P17076.

NextBio

981793.

Entry information

Entry name

RL8A_YEAST

Accession

Primary (citable) accession number: P17076
Secondary accession number(s): D3DKT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 132 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents