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P17076

- RL8A_YEAST

UniProt

P17076 - RL8A_YEAST

Protein

60S ribosomal protein L8-A

Gene

RPL8A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. maturation of LSU-rRNA Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31052-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L8-A
    Alternative name(s):
    L4
    L4-2
    L7a-1
    Maintenance of killer protein 7
    RP6
    YL5
    Gene namesi
    Name:RPL8A
    Synonyms:MAK7, RPL4A
    Ordered Locus Names:YHL033C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    SGDiS000001025. RPL8A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 25625560S ribosomal protein L8-APRO_0000136760Add
    BLAST

    Proteomic databases

    MaxQBiP17076.
    PeptideAtlasiP17076.
    PRIDEiP17076.

    Expressioni

    Gene expression databases

    GenevestigatoriP17076.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi36389. 187 interactions.
    DIPiDIP-5102N.
    IntActiP17076. 30 interactions.
    MINTiMINT-483885.
    STRINGi4932.YHL033C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-G102-220[»]
    1S1Ielectron microscopy11.70G102-220[»]
    1VW8electron microscopy6.10H1-256[»]
    1VWUelectron microscopy6.10H1-256[»]
    3IZSelectron microscopy-H1-256[»]
    3J65electron microscopy8.70H1-256[»]
    3O58X-ray4.00H1-256[»]
    3O5HX-ray4.00H1-256[»]
    3U5EX-ray3.00G1-256[»]
    3U5IX-ray3.00G1-256[»]
    4B6Aelectron microscopy8.10G1-256[»]
    4BYNelectron microscopy4.30G2-256[»]
    4BYUelectron microscopy6.60G2-256[»]
    4CUWelectron microscopy3.70G1-256[»]
    ProteinModelPortaliP17076.
    SMRiP17076. Positions 24-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP17076.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L7Ae family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000004753.
    HOGENOMiHOG000216644.
    KOiK02936.
    OMAiPSLCKKM.
    OrthoDBiEOG75QRG2.

    Family and domain databases

    Gene3Di3.30.1330.30. 1 hit.
    InterProiIPR029064. L30e-like.
    IPR001921. Ribosomal_L7A/L8.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    IPR018492. Ribosomal_L7Ae/L8/Nhp2.
    IPR004037. Ribosomal_L7Ae_CS.
    [Graphical view]
    PANTHERiPTHR23105:SF1. PTHR23105:SF1. 1 hit.
    PfamiPF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view]
    PRINTSiPR00881. L7ARS6FAMILY.
    PR00882. RIBOSOMALL7A.
    SUPFAMiSSF55315. SSF55315. 1 hit.
    PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P17076-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV    50
    KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE 100
    TAAEKKERLT KEAAAVAEGK SKQDASPKPY AVKYGLNHVV ALIENKKAKL 150
    VLIANDVDPI ELVVFLPALC KKMGVPYAIV KGKARLGTLV NQKTSAVAAL 200
    TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN KAQAKMDKRA 250
    KNSDSA 256
    Length:256
    Mass (Da):28,125
    Last modified:January 23, 2007 - v4
    Checksum:iE3C8537D8683C905
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti113 – 1131A → R in CAA35073. (PubMed:2183194)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17204 Genomic DNA. Translation: CAA35073.1.
    X56836 Genomic DNA. Translation: CAA40166.1.
    U17361 Genomic DNA. Translation: AAA64574.1.
    U11583 Genomic DNA. Translation: AAB65045.1.
    BK006934 Genomic DNA. Translation: DAA06652.1.
    PIRiS16811. R5BY7A.
    RefSeqiNP_011830.1. NM_001179113.1.

    Genome annotation databases

    EnsemblFungiiYHL033C; YHL033C; YHL033C.
    GeneIDi856352.
    KEGGisce:YHL033C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17204 Genomic DNA. Translation: CAA35073.1 .
    X56836 Genomic DNA. Translation: CAA40166.1 .
    U17361 Genomic DNA. Translation: AAA64574.1 .
    U11583 Genomic DNA. Translation: AAB65045.1 .
    BK006934 Genomic DNA. Translation: DAA06652.1 .
    PIRi S16811. R5BY7A.
    RefSeqi NP_011830.1. NM_001179113.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - G 102-220 [» ]
    1S1I electron microscopy 11.70 G 102-220 [» ]
    1VW8 electron microscopy 6.10 H 1-256 [» ]
    1VWU electron microscopy 6.10 H 1-256 [» ]
    3IZS electron microscopy - H 1-256 [» ]
    3J65 electron microscopy 8.70 H 1-256 [» ]
    3O58 X-ray 4.00 H 1-256 [» ]
    3O5H X-ray 4.00 H 1-256 [» ]
    3U5E X-ray 3.00 G 1-256 [» ]
    3U5I X-ray 3.00 G 1-256 [» ]
    4B6A electron microscopy 8.10 G 1-256 [» ]
    4BYN electron microscopy 4.30 G 2-256 [» ]
    4BYU electron microscopy 6.60 G 2-256 [» ]
    4CUW electron microscopy 3.70 G 1-256 [» ]
    ProteinModelPortali P17076.
    SMRi P17076. Positions 24-256.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36389. 187 interactions.
    DIPi DIP-5102N.
    IntActi P17076. 30 interactions.
    MINTi MINT-483885.
    STRINGi 4932.YHL033C.

    Proteomic databases

    MaxQBi P17076.
    PeptideAtlasi P17076.
    PRIDEi P17076.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHL033C ; YHL033C ; YHL033C .
    GeneIDi 856352.
    KEGGi sce:YHL033C.

    Organism-specific databases

    SGDi S000001025. RPL8A.

    Phylogenomic databases

    GeneTreei ENSGT00390000004753.
    HOGENOMi HOG000216644.
    KOi K02936.
    OMAi PSLCKKM.
    OrthoDBi EOG75QRG2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31052-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P17076.
    NextBioi 981793.

    Gene expression databases

    Genevestigatori P17076.

    Family and domain databases

    Gene3Di 3.30.1330.30. 1 hit.
    InterProi IPR029064. L30e-like.
    IPR001921. Ribosomal_L7A/L8.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    IPR018492. Ribosomal_L7Ae/L8/Nhp2.
    IPR004037. Ribosomal_L7Ae_CS.
    [Graphical view ]
    PANTHERi PTHR23105:SF1. PTHR23105:SF1. 1 hit.
    Pfami PF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view ]
    PRINTSi PR00881. L7ARS6FAMILY.
    PR00882. RIBOSOMALL7A.
    SUPFAMi SSF55315. SSF55315. 1 hit.
    PROSITEi PS01082. RIBOSOMAL_L7AE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a."
      Yon J., Giallongo A., Fried M.
      Mol. Gen. Genet. 227:72-80(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Ribosomal protein L4 of Saccharomyces cerevisiae: the gene and its protein."
      Arevalo S.G., Warner J.R.
      Nucleic Acids Res. 18:1447-1450(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204626 / S288c / A364A.
    3. "Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast."
      Wickner R.B., Leibowitz M.J.
      J. Mol. Biol. 105:427-443(1976) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
      Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
      J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-20.
    7. "Yeast virus propagation depends critically on free 60S ribosomal subunit concentration."
      Ohtake Y., Wickner R.B.
      Mol. Cell. Biol. 15:2772-2781(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION AS MAK7.
    8. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    9. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
    13. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
    14. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    15. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL8A_YEAST
    AccessioniPrimary (citable) accession number: P17076
    Secondary accession number(s): D3DKT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 143 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 238000 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for L8 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3