60S ribosomal protein L8-A - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P17076 (RL8A_YEAST)

Last modified November 24, 2009. Version 101. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    60S ribosomal protein L8-A
Alternative name(s):
    L4
    L4-2
    YL5
    RP6
    L7a-1
    Maintenance of killer protein 7

Gene names

Name:	RPL8A
Synonyms:	MAK7, RPL4A
Ordered Locus Names:	YHL033C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7
Subcellular location	Cytoplasm.
Miscellaneous	Present with 238000 molecules/cell in log phase SD medium. Ref.10 There are 2 genes for L8 in yeast.
Sequence similarities	Belongs to the ribosomal protein L7Ae family.

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Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ribosome biogenesis Inferred from electronic annotation. Source: InterPro translation Ref.7 Traceable author statement. Source: SGD
Cellular component	cytosolic large ribosomal subunit Ref.7 Traceable author statement. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of ribosome Ref.7 Traceable author statement. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	1	EBI-15431,EBI-16219
SSM4	P40318	1	EBI-15431,EBI-18208

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5 Ref.8

Chain

2 – 256

255

60S ribosomal protein L8-A

PRO_0000136760

Amino acid modifications

Modified residue

126

Phosphoserine Ref.11 Ref.13

Modified residue

216

Phosphoserine Ref.13 Ref.12

Experimental info

Sequence conflict

113

A → R in CAA35073. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P17076-1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: E3C8537D8683C905
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FASTA

256

28,125

        10         20         30         40         50         60 
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR 

        70         80         90        100        110        120 
QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE TAAEKKERLT KEAAAVAEGK 

       130        140        150        160        170        180 
SKQDASPKPY AVKYGLNHVV ALIENKKAKL VLIANDVDPI ELVVFLPALC KKMGVPYAIV 

       190        200        210        220        230        240 
KGKARLGTLV NQKTSAVAAL TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN 

       250 
KAQAKMDKRA KNSDSA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The organization and expression of the Saccharomyces cerevisiae L4 ribosomal protein genes and their identification as the homologues of the mammalian ribosomal protein gene L7a." Yon J., Giallongo A., Fried M. Mol. Gen. Genet. 227:72-80(1991) [PubMed: 2046660] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Ribosomal protein L4 of Saccharomyces cerevisiae: the gene and its protein." Arevalo S.G., Warner J.R. Nucleic Acids Res. 18:1447-1450(1990) [PubMed: 2183194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204626 / S288c / A364A.
[3]	"Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast." Wickner R.B., Leibowitz M.J. J. Mol. Biol. 105:427-443(1976) [PubMed: 787537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed: 1544921] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20.
[6]	"Yeast virus propagation depends critically on free 60S ribosomal subunit concentration." Ohtake Y., Wickner R.B. Mol. Cell. Biol. 15:2772-2781(1995) [PubMed: 7739558] [Abstract] Cited for: IDENTIFICATION AS MAK7.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126, MASS SPECTROMETRY.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126 AND SER-216, MASS SPECTROMETRY.
[14]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
[15]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 102-220, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X17204 Genomic DNA. Translation: CAA35073.1.
X56836 Genomic DNA. Translation: CAA40166.1.
U17361 Genomic DNA. Translation: AAA64574.1.
U11583 Genomic DNA. Translation: AAB65045.1.

PIR

R5BY7A. S16811.

RefSeq

NP_011830.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	G	102-220	[»]
1S1I	electron microscopy	11.70	G	102-220	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:5102N.

IntAct

P17076. 31 interactions.

STRING

P17076.

Proteomic databases

PeptideAtlas

P17076.

PRIDE

P17076.

Genome annotation databases

Ensembl

YHL033C; YHL033C; YHL033C; Saccharomyces cerevisiae. [Genome view]

GeneID

856352.

KEGG

sce:YHL033C.

NMPDR

fig|4932.3.peg.2967.

Organism-specific databases

CYGD

YHL033c.

SGD

S000001025. RPL8A.

Phylogenomic databases

HOGENOM

P17076.

OMA

RREDEAQ

OrthoDB

EOG9HB02T

Gene expression databases

ArrayExpress

P17076.

Genevestigator

P17076.

GermOnline

YHL033C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001921. Ribosomal_L7A.
IPR018492. Ribosomal_L7A/RS6_fam.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR004037. Ribosomal_L7Ae_CS.
[Graphical view]

PANTHER

PTHR23105:SF1. Ribosomal_L7A. 1 hit.

Pfam

PF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]

PRINTS

PR00881. L7ARS6FAMILY.
PR00882. RIBOSOMALL7A.

PROSITE

PS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

981793.

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Entry information

Entry name

RL8A_YEAST

Accession

Primary (citable) accession number: P17076

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 101 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents