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Protein

Isocitrate lyase

Gene
N/A
Organism
Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in storage lipid mobilization during the growth of higher plant seedling.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi175 – 1751MagnesiumBy similarity
Active sitei213 – 2131Proton acceptorBy similarity
Binding sitei250 – 2501SubstrateBy similarity
Binding sitei472 – 4721SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.1.3.1. 2499.
UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyaseBy similarity (EC:4.1.3.1By similarity)
Short name:
ICLBy similarity
Alternative name(s):
IsocitraseBy similarity
IsocitratsysaseBy similarity
OrganismiGossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Taxonomic identifieri3635 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMalvalesMalvaceaeMalvoideaeGossypium

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Isocitrate lyasePRO_0000068806Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP17069.
SMRiP17069. Positions 9-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 1063Substrate bindingBy similarity
Regioni214 – 2152Substrate bindingBy similarity
Regioni437 – 4415Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi574 – 5763Microbody targeting signalSequence Analysis

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASFSVPSM IMEEEGRFET EVAEVQAWWN SERFKLTRRP YSARDVVALR
60 70 80 90 100
GSLKQSYGSN EMAKKLWTTL KTHQANGTAS RTFGALDPVQ VTMMAKHLDS
110 120 130 140 150
IYVSGWQCSS THTTTNEPGP DLADYPYDTV PNKVEHLFFA QQYHDRKQRE
160 170 180 190 200
ARMSMSREER ARTPYVDYLK PIIADGDTGF GGTTATVKLC KLFVERGAAG
210 220 230 240 250
VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV MGVETVLVAR
260 270 280 290 300
TDAVAATLIQ TNVDTRDHQF ILGATNPNLR GKSLANMLAE GMAAGKNGPQ
310 320 330 340 350
LQAIEDNWLA IAQLKTFSEC VMDAIKSMNI TEDEKRRRMN EWMNHSSYDK
360 370 380 390 400
CLSNEQAREI AERLGLQNLF WDWDLPRTRE GFYRFRGSVM AAIVRGWAFA
410 420 430 440 450
PHADLIWMET SSPDMVECTR FAEGVKSMHP EIMLAYNLSP SFNWDASGMT
460 470 480 490 500
DEHMRDFIPR IAKLGFCWQF ITLAGFHADA LVTDTFARDF ARRGMLAYVE
510 520 530 540 550
KIQREERNNG VDTLAHQKWS GANFYDRYLK TVQGGISSTA AMGKGVTEEQ
560 570
FKETWTRPGA GNIGSEGNLV VAKARM
Length:576
Mass (Da):64,733
Last modified:August 1, 1990 - v1
Checksum:i84D211AA3DCE8700
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52136 mRNA. Translation: CAA36381.1.
PIRiS10771. WZCNIU.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52136 mRNA. Translation: CAA36381.1.
PIRiS10771. WZCNIU.

3D structure databases

ProteinModelPortaliP17069.
SMRiP17069. Positions 9-535.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.
BRENDAi4.1.3.1. 2499.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of a cDNA clone encoding the complete amino acid sequence of cotton isocitrate lyase."
    Turley R.B., Choe S.M., Trelease R.N.
    Biochim. Biophys. Acta 1049:223-226(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Deltapine 62.
    Tissue: Cotyledon.

Entry informationi

Entry nameiACEA_GOSHI
AccessioniPrimary (citable) accession number: P17069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 29, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.