Reviewed, UniProtKB/Swiss-Prot P17065 (SEC2_YEAST)

Names and origin

Protein names

Recommended name:
    Rab guanine nucleotide exchange factor SEC2
Alternative name(s):
    GDP-GTP exchange factor SEC2

Gene names

Name:	SEC2
Ordered Locus Names:	YNL272C
ORF Names:	N0641

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Guanine nucleotide exchange factor for SEC4, catalyzing the dissociation of GDP from SEC4 and also potently promoting binding of GTP. Activation of SEC4 by SEC2 is needed for the directed transport of vesicles to sites of exocytosis. Binds the Rab GTPase YPT32, but does not have exhange activity on YPT32. Ref.3 Ref.4 Ref.5
Subunit structure	Interacts with SEC4. Interacts with YPT32, preferentially in its GTP-bound form. Ref.3 Ref.5
Subcellular location	Bud neck. Bud tip. Cytoplasmic vesicle › secretory vesicle. Note: Localizes to sites of polarized growth, namely to the bud neck and to the bud tip of growing buds, and associates with membranes. Colocalizes with secretory vesicles at exocytic sites. Proper localization is dependent on the actin cytoskeleton, MYO2, the kinesin-related protein SMY1, the Rab GTPase YPT32, and the production of post-Golgi vesicles. Ref.4
Domain	The N-terminal domain (1-374) is sufficient for the exchange factor activity.
Post-translational modification	Phosphorylated. Ref.4 Ref.7 Ref.8 Ref.9
Miscellaneous	Present with 13200 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the SEC2 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	exocytosis Ref.5 Inferred from mutant phenotype. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cellular bud neck Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud tip Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Ref.5 Inferred from direct assay. Source: SGD mating projection tip Inferred from direct assay. Source: SGD secretory granule Inferred from electronic annotation. Source: UniProtKB-SubCell transport vesicle Ref.5 Inferred from direct assay. Source: SGD
Molecular function	guanyl-nucleotide exchange factor activity Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 759

759

Rab guanine nucleotide exchange factor SEC2

PRO_0000097661

Regions

Region

451 – 508

58

Required for proper polarized localization of the protein

Coiled coil

26 – 164

139

Potential

Coiled coil

651 – 682

32

Potential

Coiled coil

732 – 759

28

Potential

Amino acid modifications

Modified residue

197

1

Phosphoserine Ref.9

Modified residue

200

1

Phosphoserine Ref.9

Modified residue

204

1

Phosphotyrosine Ref.9

Modified residue

409

1

Phosphothreonine Ref.9

Modified residue

515

1

Phosphoserine Ref.7 Ref.9

Modified residue

524

1

Phosphoserine Ref.7

Modified residue

544

1

Phosphoserine Ref.8

Modified residue

545

1

Phosphoserine Ref.8

Experimental info

Mutagenesis

483

1

C → Y in SEC2-78; temperature-sensitive; causes mislocalization of the protein, displaying many cytoplasmic punctae. Ref.4

Secondary structure

1

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.

.

759

Helix Strand Turn

Details...

Helix

17 – 30

14

Turn

34 – 36

3

Helix

37 – 65

29

Turn

66 – 68

3

Helix

69 – 74

6

Helix

76 – 82

7

Helix

86 – 92

7

Helix

95 – 118

24

Helix

121 – 135

15

Turn

136 – 138

3

Helix

139 – 142

4

Sequences

	Sequence		Length	Mass (Da)
	P17065-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 7F5A9B2343CBE835	FASTA	759	84,652
	10 20 30 40 50 60 MDASEEAKRV SIQVTSLSTQ LIESVDKQSH LEEQLNKSLK TIASQKAAIE NYNQLKEDYN 70 80 90 100 110 120 TLKRELSDRD DEVKRLREDI AKENELRTKA EEEADKLNKE VEDLTASLFD EANNMVADAR 130 140 150 160 170 180 KEKYAIEILN KRLTEQLREK DTLLDTLTLQ LKNLKKVMHS LDNESTVTNN SNRYSTILSD 190 200 210 220 230 240 SATSSSTSLN KVPTSYSLAS QDIYSGIVYS PSISSIRYDI SLYNEFLKFV AALPRCENIK 250 260 270 280 290 300 ATSTESKLIR RLVNDEIQPI LKIDNASGIG WLVKKTLLSL IIDGLVVVEP LSGVNATYQI 310 320 330 340 350 360 GYNSSSPAKQ ATSNMPKMFK FPLDSPPVAV HAACSFCGES RDDIIEHARM YILKTLHKTD 370 380 390 400 410 420 DGKEQVTNTY PLCHWCLLKL RQTCEIFAFL RSLKVGAWHL EKLTTQNITK EDLEKFSEVT 430 440 450 460 470 480 KHTKRDGRVS SQDKKTKRLS FMAGLGINSS TKNKPKMEIF SSETNAKPGQ PTTNIQRAWL 490 500 510 520 530 540 QLCKLRCILH WTHIGIWAVD DSISSKIGPL VEDDSDEDQN DAISVRLQDK ALWKQDAKRP 550 560 570 580 590 600 FSSSSAEESQ KSDAFDFESG DMENEITGES SSDESSSDGS STDNSTADSS SEDESSLADS 610 620 630 640 650 660 TTSSADSSSP ESIDNGEGDD TVTKDDKSSI KSANNNEENS DCGDKKGRSI IKKKAPQRKI 670 680 690 700 710 720 QKKKLLQDLD DLEEQFREES AIDQTEFENA ESNVKQNISS KRASSGDENS KKDNNEKTLK 730 740 750 TNLTIGDKTQ EQIGENSPSS GLHASSSNDD NFDDAQEQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sec2 protein contains a coiled-coil domain essential for vesicular transport and a dispensable carboxy terminal domain." Nair J., Mueller H., Peterson M., Novick P.J. J. Cell Biol. 110:1897-1909(1990) [PubMed: 1693620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles." Walch-Solimena C., Collins R.N., Novick P.J. J. Cell Biol. 137:1495-1509(1997) [PubMed: 9199166] [Abstract] Cited for: FUNCTION, INTERACTION WITH SEC4.
[4]	"The role of the COOH terminus of Sec2p in the transport of post-Golgi vesicles." Elkind N.B., Walch-Solimena C., Novick P.J. J. Cell Biol. 149:95-110(2000) [PubMed: 10747090] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-483, PHOSPHORYLATION.
[5]	"Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast." Ortiz D., Medkova M., Walch-Solimena C., Novick P.J. J. Cell Biol. 157:1005-1015(2002) [PubMed: 12045183] [Abstract] Cited for: FUNCTION, INTERACTION WITH YPT32.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-515 AND SER-524, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-544 AND SER-545, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-197; SER-200; TYR-204; THR-409 AND SER-515, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X52147 Genomic DNA. Translation: CAA36395.1.
Z71548 Genomic DNA. Translation: CAA96180.1.

PIR

A35597.

RefSeq

NP_014127.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2E7S	X-ray	3.00	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T	31-160	[»]
2EQB	X-ray	2.70	B/C	51-142	[»]
2OCY	X-ray	3.30	A/B	17-167	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2493N.

IntAct

P17065. 29 interactions.

STRING

P17065.

Genome annotation databases

Ensembl

YNL272C; YNL272C; YNL272C; Saccharomyces cerevisiae. [Genome view]

GeneID

855449.

KEGG

sce:YNL272C.

NMPDR

fig|4932.3.peg.5192.

Organism-specific databases

CYGD

YNL272c.

SGD

S000005216. SEC2.

Phylogenomic databases

HOGENOM

P17065.

OMA

STQLIES

OrthoDB

EOG9Z37WV

Gene expression databases

ArrayExpress

P17065.

Genevestigator

P17065.

GermOnline

YNL272C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR009449. Sec2p.
[Graphical view]

Pfam

PF06428. Sec2p. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

979355.

Entry information

Entry name

SEC2_YEAST

Accession

Primary (citable) accession number: P17065

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	November 24, 2009
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)