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Protein

Purine-cytosine permease FCY2

Gene

FCY2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This permease has a broad specificity towards purines, and also transport cytosine and 5-methylcytosine but neither uracil nor thymine.

GO - Molecular functioni

  • cytidine transmembrane transporter activity Source: SGD
  • nucleobase transmembrane transporter activity Source: SGD

GO - Biological processi

  • cytidine transport Source: SGD
  • cytosine transport Source: SGD
  • purine-containing compound transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30233-MONOMER.

Protein family/group databases

TCDBi2.A.39.2.1. the nucleobase:cation symporter-1 (ncs1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine-cytosine permease FCY2
Short name:
PCP FCY2
Alternative name(s):
Cytosine/purine transport protein FCY2
Fluorocytosine resistance protein 2
Gene namesi
Name:FCY2
Ordered Locus Names:YER056C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER056C.
SGDiS000000858. FCY2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 98CytoplasmicSequence analysisAdd BLAST98
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Topological domaini120 – 121ExtracellularSequence analysis2
Transmembranei122 – 141HelicalSequence analysisAdd BLAST20
Topological domaini142 – 198CytoplasmicSequence analysisAdd BLAST57
Transmembranei199 – 218HelicalSequence analysisAdd BLAST20
Topological domaini219 – 256ExtracellularSequence analysisAdd BLAST38
Transmembranei257 – 276HelicalSequence analysisAdd BLAST20
Topological domaini277 – 300CytoplasmicSequence analysisAdd BLAST24
Transmembranei301 – 320HelicalSequence analysisAdd BLAST20
Topological domaini321 – 347ExtracellularSequence analysisAdd BLAST27
Transmembranei348 – 367HelicalSequence analysisAdd BLAST20
Topological domaini368 – 398CytoplasmicSequence analysisAdd BLAST31
Transmembranei399 – 418HelicalSequence analysisAdd BLAST20
Topological domaini419 – 465ExtracellularSequence analysisAdd BLAST47
Transmembranei466 – 485HelicalSequence analysisAdd BLAST20
Topological domaini486 – 533CytoplasmicSequence analysisAdd BLAST48

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001979201 – 533Purine-cytosine permease FCY2Add BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki16Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei18PhosphoserineCombined sources1

Post-translational modificationi

Not N-glycosylated.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP17064.
PRIDEiP17064.

PTM databases

iPTMnetiP17064.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PHO88P382643EBI-2047850,EBI-13350

Protein-protein interaction databases

BioGridi36796. 45 interactors.
DIPiDIP-7593N.
IntActiP17064. 4 interactors.
MINTiMINT-1362074.

Structurei

3D structure databases

ProteinModelPortaliP17064.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 184Surface seekingSequence analysisAdd BLAST20

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004423.
HOGENOMiHOG000162981.
InParanoidiP17064.
OMAiINIASDW.
OrthoDBiEOG092C1MEG.

Family and domain databases

InterProiIPR012681. NCS1.
IPR001248. Pur-cyt_permease.
IPR026030. Pur-cyt_permease_Fcy2/21/22.
[Graphical view]
PfamiPF02133. Transp_cyt_pur. 1 hit.
[Graphical view]
PIRSFiPIRSF002744. Pur-cyt_permease. 1 hit.
TIGRFAMsiTIGR00800. ncs1. 1 hit.

Sequencei

Sequence statusi: Complete.

P17064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEEGNNVYE IQDLEKRSPV IGSSLENEKK VAASETFTAT SEDDQQYIVE
60 70 80 90 100
SSEATKLSWF HKFFASLNAE TKGVEPVTED EKTDDSILNA ASMWFSANMV
110 120 130 140 150
IASYALGALG PMVFGLNFGQ SVLVIIFFNI MGLIFVAFFS VFGAELGLRQ
160 170 180 190 200
MILSRYLVGN VTARIFSLIN VIACVGWGIV NTSVSAQLLN MVNEGSGHVC
210 220 230 240 250
PIWAGCLIII GGTVLVTFFG YSVIHAYEKW SWVPNFAVFL VIIAQLSRSG
260 270 280 290 300
KFKGGEWVGG ATTAGSVLSF GSSIFGFAAG WTTYAADYTV YMPKSTNKYK
310 320 330 340 350
IFFSLVAGLA FPLFFTMILG AASAMAALND PTWKAYYDKN AMGGVIYAIL
360 370 380 390 400
VPNSLNGFGQ FCCVLLALST IANNIPNMYT VALSAQALWA PLAKIPRVVW
410 420 430 440 450
TMAGNAATLG ISIPATYYFD GFMENFMDSI GYYLAIYIAI SCSEHFFYRR
460 470 480 490 500
SFSAYNIDDW DNWEHLPIGI AGTAALIVGA FGVALGMCQT YWVGEIGRLI
510 520 530
GKYGGDIGFE LGASWAFIIY NILRPLELKY FGR
Length:533
Mass (Da):58,201
Last modified:February 1, 1995 - v2
Checksum:i762DDB9483A546AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192V → M in CAA36040 (PubMed:2191181).Curated1
Sequence conflicti261A → G in CAA36040 (PubMed:2191181).Curated1
Sequence conflicti459D → Y in V01306 (PubMed:6300123).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51751 Genomic DNA. Translation: CAA36040.1.
U18813 Genomic DNA. Translation: AAB64592.1.
V01306 Genomic DNA. No translation available.
BK006939 Genomic DNA. Translation: DAA07713.1.
PIRiS50559. GRBYCP.
RefSeqiNP_010976.1. NM_001178947.1.

Genome annotation databases

EnsemblFungiiYER056C; YER056C; YER056C.
GeneIDi856783.
KEGGisce:YER056C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51751 Genomic DNA. Translation: CAA36040.1.
U18813 Genomic DNA. Translation: AAB64592.1.
V01306 Genomic DNA. No translation available.
BK006939 Genomic DNA. Translation: DAA07713.1.
PIRiS50559. GRBYCP.
RefSeqiNP_010976.1. NM_001178947.1.

3D structure databases

ProteinModelPortaliP17064.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36796. 45 interactors.
DIPiDIP-7593N.
IntActiP17064. 4 interactors.
MINTiMINT-1362074.

Protein family/group databases

TCDBi2.A.39.2.1. the nucleobase:cation symporter-1 (ncs1) family.

PTM databases

iPTMnetiP17064.

Proteomic databases

MaxQBiP17064.
PRIDEiP17064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER056C; YER056C; YER056C.
GeneIDi856783.
KEGGisce:YER056C.

Organism-specific databases

EuPathDBiFungiDB:YER056C.
SGDiS000000858. FCY2.

Phylogenomic databases

GeneTreeiENSGT00390000004423.
HOGENOMiHOG000162981.
InParanoidiP17064.
OMAiINIASDW.
OrthoDBiEOG092C1MEG.

Enzyme and pathway databases

BioCyciYEAST:G3O-30233-MONOMER.

Miscellaneous databases

PROiP17064.

Family and domain databases

InterProiIPR012681. NCS1.
IPR001248. Pur-cyt_permease.
IPR026030. Pur-cyt_permease_Fcy2/21/22.
[Graphical view]
PfamiPF02133. Transp_cyt_pur. 1 hit.
[Graphical view]
PIRSFiPIRSF002744. Pur-cyt_permease. 1 hit.
TIGRFAMsiTIGR00800. ncs1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFCY2_YEAST
AccessioniPrimary (citable) accession number: P17064
Secondary accession number(s): D3DLV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 23600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.