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Protein

Purine-cytosine permease FCY2

Gene

FCY2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This permease has a broad specificity towards purines, and also transport cytosine and 5-methylcytosine but neither uracil nor thymine.

GO - Molecular functioni

  • cytidine transmembrane transporter activity Source: SGD
  • nucleobase transmembrane transporter activity Source: SGD

GO - Biological processi

  • cytidine transport Source: SGD
  • cytosine transport Source: SGD
  • purine-containing compound transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30233-MONOMER.

Protein family/group databases

TCDBi2.A.39.2.1. the nucleobase:cation symporter-1 (ncs1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine-cytosine permease FCY2
Short name:
PCP FCY2
Alternative name(s):
Cytosine/purine transport protein FCY2
Fluorocytosine resistance protein 2
Gene namesi
Name:FCY2
Ordered Locus Names:YER056C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER056C.
SGDiS000000858. FCY2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9898CytoplasmicSequence analysisAdd
BLAST
Transmembranei99 – 11921HelicalSequence analysisAdd
BLAST
Topological domaini120 – 1212ExtracellularSequence analysis
Transmembranei122 – 14120HelicalSequence analysisAdd
BLAST
Topological domaini142 – 19857CytoplasmicSequence analysisAdd
BLAST
Transmembranei199 – 21820HelicalSequence analysisAdd
BLAST
Topological domaini219 – 25638ExtracellularSequence analysisAdd
BLAST
Transmembranei257 – 27620HelicalSequence analysisAdd
BLAST
Topological domaini277 – 30024CytoplasmicSequence analysisAdd
BLAST
Transmembranei301 – 32020HelicalSequence analysisAdd
BLAST
Topological domaini321 – 34727ExtracellularSequence analysisAdd
BLAST
Transmembranei348 – 36720HelicalSequence analysisAdd
BLAST
Topological domaini368 – 39831CytoplasmicSequence analysisAdd
BLAST
Transmembranei399 – 41820HelicalSequence analysisAdd
BLAST
Topological domaini419 – 46547ExtracellularSequence analysisAdd
BLAST
Transmembranei466 – 48520HelicalSequence analysisAdd
BLAST
Topological domaini486 – 53348CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Purine-cytosine permease FCY2PRO_0000197920Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181PhosphoserineCombined sources

Post-translational modificationi

Not N-glycosylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP17064.
PeptideAtlasiP17064.

PTM databases

iPTMnetiP17064.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PHO88P382643EBI-2047850,EBI-13350

Protein-protein interaction databases

BioGridi36796. 45 interactions.
DIPiDIP-7593N.
IntActiP17064. 4 interactions.
MINTiMINT-1362074.

Structurei

3D structure databases

ProteinModelPortaliP17064.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni165 – 18420Surface seekingSequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004423.
HOGENOMiHOG000162981.
InParanoidiP17064.
OMAiSANMVIA.
OrthoDBiEOG75QRD0.

Family and domain databases

InterProiIPR012681. NCS1.
IPR001248. Pur-cyt_permease.
IPR026030. Pur-cyt_permease_Fcy2/21/22.
[Graphical view]
PfamiPF02133. Transp_cyt_pur. 1 hit.
[Graphical view]
PIRSFiPIRSF002744. Pur-cyt_permease. 1 hit.
TIGRFAMsiTIGR00800. ncs1. 1 hit.

Sequencei

Sequence statusi: Complete.

P17064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEEGNNVYE IQDLEKRSPV IGSSLENEKK VAASETFTAT SEDDQQYIVE
60 70 80 90 100
SSEATKLSWF HKFFASLNAE TKGVEPVTED EKTDDSILNA ASMWFSANMV
110 120 130 140 150
IASYALGALG PMVFGLNFGQ SVLVIIFFNI MGLIFVAFFS VFGAELGLRQ
160 170 180 190 200
MILSRYLVGN VTARIFSLIN VIACVGWGIV NTSVSAQLLN MVNEGSGHVC
210 220 230 240 250
PIWAGCLIII GGTVLVTFFG YSVIHAYEKW SWVPNFAVFL VIIAQLSRSG
260 270 280 290 300
KFKGGEWVGG ATTAGSVLSF GSSIFGFAAG WTTYAADYTV YMPKSTNKYK
310 320 330 340 350
IFFSLVAGLA FPLFFTMILG AASAMAALND PTWKAYYDKN AMGGVIYAIL
360 370 380 390 400
VPNSLNGFGQ FCCVLLALST IANNIPNMYT VALSAQALWA PLAKIPRVVW
410 420 430 440 450
TMAGNAATLG ISIPATYYFD GFMENFMDSI GYYLAIYIAI SCSEHFFYRR
460 470 480 490 500
SFSAYNIDDW DNWEHLPIGI AGTAALIVGA FGVALGMCQT YWVGEIGRLI
510 520 530
GKYGGDIGFE LGASWAFIIY NILRPLELKY FGR
Length:533
Mass (Da):58,201
Last modified:February 1, 1995 - v2
Checksum:i762DDB9483A546AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921V → M in CAA36040 (PubMed:2191181).Curated
Sequence conflicti261 – 2611A → G in CAA36040 (PubMed:2191181).Curated
Sequence conflicti459 – 4591D → Y in V01306 (PubMed:6300123).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51751 Genomic DNA. Translation: CAA36040.1.
U18813 Genomic DNA. Translation: AAB64592.1.
V01306 Genomic DNA. No translation available.
BK006939 Genomic DNA. Translation: DAA07713.1.
PIRiS50559. GRBYCP.
RefSeqiNP_010976.1. NM_001178947.1.

Genome annotation databases

EnsemblFungiiYER056C; YER056C; YER056C.
GeneIDi856783.
KEGGisce:YER056C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51751 Genomic DNA. Translation: CAA36040.1.
U18813 Genomic DNA. Translation: AAB64592.1.
V01306 Genomic DNA. No translation available.
BK006939 Genomic DNA. Translation: DAA07713.1.
PIRiS50559. GRBYCP.
RefSeqiNP_010976.1. NM_001178947.1.

3D structure databases

ProteinModelPortaliP17064.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36796. 45 interactions.
DIPiDIP-7593N.
IntActiP17064. 4 interactions.
MINTiMINT-1362074.

Protein family/group databases

TCDBi2.A.39.2.1. the nucleobase:cation symporter-1 (ncs1) family.

PTM databases

iPTMnetiP17064.

Proteomic databases

MaxQBiP17064.
PeptideAtlasiP17064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER056C; YER056C; YER056C.
GeneIDi856783.
KEGGisce:YER056C.

Organism-specific databases

EuPathDBiFungiDB:YER056C.
SGDiS000000858. FCY2.

Phylogenomic databases

GeneTreeiENSGT00390000004423.
HOGENOMiHOG000162981.
InParanoidiP17064.
OMAiSANMVIA.
OrthoDBiEOG75QRD0.

Enzyme and pathway databases

BioCyciYEAST:G3O-30233-MONOMER.

Miscellaneous databases

PROiP17064.

Family and domain databases

InterProiIPR012681. NCS1.
IPR001248. Pur-cyt_permease.
IPR026030. Pur-cyt_permease_Fcy2/21/22.
[Graphical view]
PfamiPF02133. Transp_cyt_pur. 1 hit.
[Graphical view]
PIRSFiPIRSF002744. Pur-cyt_permease. 1 hit.
TIGRFAMsiTIGR00800. ncs1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The purine-cytosine permease gene of Saccharomyces cerevisiae: primary structure and deduced protein sequence of the FCY2 gene product."
    Weber E., Rodriguez C., Chevalier M.R., Jund R.
    Mol. Microbiol. 4:585-596(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae."
    Hinnebusch A.G., Fink G.R.
    J. Biol. Chem. 258:5238-5247(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 380-533.
  5. "The immunodetected yeast purine-cytosine permease is not N-linked glycosylated, nor are glycosylation sequences required to have a functional permease."
    Rodriguez C., Bloch J.C., Chevalier M.R.
    Yeast 11:15-23(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFCY2_YEAST
AccessioniPrimary (citable) accession number: P17064
Secondary accession number(s): D3DLV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 23600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.