Purine-cytosine permease FCY2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P17064 (FCY2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Purine-cytosine permease FCY2
Short name=PCP FCY2

Alternative name(s):
Cytosine/purine transport protein FCY2
Fluorocytosine resistance protein 2

Gene names

Name:	FCY2
Ordered Locus Names:	YER056C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This permease has a broad specificity towards purines, and also transport cytosine and 5-methylcytosine but neither uracil nor thymine.
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	Not N-glycosylated.
Miscellaneous	Present with 23600 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the purine-cytosine permease family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytosine transport Inferred from direct assay. Source: SGD purine base transport Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: SGD
Molecular function	cytidine transmembrane transporter activity Inferred from mutant phenotype. Source: SGD nucleobase transmembrane transporter activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
AGP1	P25376	1	EBI-2047850,EBI-2357
CAN1	P04817	1	EBI-2047850,EBI-3993
CHO1	P08456	1	EBI-2047850,EBI-14055
GAP1	P19145	1	EBI-2047850,EBI-7314
PHO88	P38264	1	EBI-2047850,EBI-13350
SOP4	P39543	1	EBI-2047850,EBI-26193
SPF1	P39986	1	EBI-2047850,EBI-3128

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 533

533

Purine-cytosine permease FCY2

PRO_0000197920

Regions

Topological domain

1 – 98

Cytoplasmic Potential

Transmembrane

99 – 119

Helical; Potential

Topological domain

120 – 121

Extracellular Potential

Transmembrane

122 – 141

Helical; Potential

Topological domain

142 – 198

Cytoplasmic Potential

Transmembrane

199 – 218

Helical; Potential

Topological domain

219 – 256

Extracellular Potential

Transmembrane

257 – 276

Helical; Potential

Topological domain

277 – 300

Cytoplasmic Potential

Transmembrane

301 – 320

Helical; Potential

Topological domain

321 – 347

Extracellular Potential

Transmembrane

348 – 367

Helical; Potential

Topological domain

368 – 398

Cytoplasmic Potential

Transmembrane

399 – 418

Helical; Potential

Topological domain

419 – 465

Extracellular Potential

Transmembrane

466 – 485

Helical; Potential

Topological domain

486 – 533

Cytoplasmic Potential

Region

165 – 184

Surface seeking Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Experimental info

Sequence conflict

192

V → M in CAA36040. Ref.1

Sequence conflict

261

A → G in CAA36040. Ref.1

Sequence conflict

459

D → Y in V01306. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P17064 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 762DDB9483A546AC
Show »

FASTA

533

58,201

        10         20         30         40         50         60 
MLEEGNNVYE IQDLEKRSPV IGSSLENEKK VAASETFTAT SEDDQQYIVE SSEATKLSWF 

        70         80         90        100        110        120 
HKFFASLNAE TKGVEPVTED EKTDDSILNA ASMWFSANMV IASYALGALG PMVFGLNFGQ 

       130        140        150        160        170        180 
SVLVIIFFNI MGLIFVAFFS VFGAELGLRQ MILSRYLVGN VTARIFSLIN VIACVGWGIV 

       190        200        210        220        230        240 
NTSVSAQLLN MVNEGSGHVC PIWAGCLIII GGTVLVTFFG YSVIHAYEKW SWVPNFAVFL 

       250        260        270        280        290        300 
VIIAQLSRSG KFKGGEWVGG ATTAGSVLSF GSSIFGFAAG WTTYAADYTV YMPKSTNKYK 

       310        320        330        340        350        360 
IFFSLVAGLA FPLFFTMILG AASAMAALND PTWKAYYDKN AMGGVIYAIL VPNSLNGFGQ 

       370        380        390        400        410        420 
FCCVLLALST IANNIPNMYT VALSAQALWA PLAKIPRVVW TMAGNAATLG ISIPATYYFD 

       430        440        450        460        470        480 
GFMENFMDSI GYYLAIYIAI SCSEHFFYRR SFSAYNIDDW DNWEHLPIGI AGTAALIVGA 

       490        500        510        520        530 
FGVALGMCQT YWVGEIGRLI GKYGGDIGFE LGASWAFIIY NILRPLELKY FGR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The purine-cytosine permease gene of Saccharomyces cerevisiae: primary structure and deduced protein sequence of the FCY2 gene product." Weber E., Rodriguez C., Chevalier M.R., Jund R. Mol. Microbiol. 4:585-596(1990) [PubMed: 2191181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae." Hinnebusch A.G., Fink G.R. J. Biol. Chem. 258:5238-5247(1983) [PubMed: 6300123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 380-533.
[5]	"The immunodetected yeast purine-cytosine permease is not N-linked glycosylated, nor are glycosylation sequences required to have a functional permease." Rodriguez C., Bloch J.C., Chevalier M.R. Yeast 11:15-23(1995) [PubMed: 7762297] [Abstract] Cited for: MUTAGENESIS.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X51751 Genomic DNA. Translation: CAA36040.1. U18813 Genomic DNA. Translation: AAB64592.1. V01306 Genomic DNA. No translation available. BK006939 Genomic DNA. Translation: DAA07713.1.
PIR	GRBYCP. S50559.
RefSeq	NP_010976.1. NM_001178947.1.
3D structure databases
ProteinModelPortal	P17064.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7593N.
IntAct	P17064. 7 interactions.
MINT	MINT-1362074.
STRING	P17064.
Protein family/group databases
TCDB	2.A.39.2.1. nucleobase:cation symporter-1 (NCS1) family.
Proteomic databases
PeptideAtlas	P17064.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER056C; YER056C; YER056C.
GeneID	856783.
KEGG	sce:YER056C.
NMPDR	fig\|4932.3.peg.2040.
Organism-specific databases
CYGD	YER056c.
SGD	S000000858. FCY2.
Phylogenomic databases
eggNOG	fuNOG05221.
GeneTree	EFGT00050000001818.
HOGENOM	HBG396658.
OMA	PNIYSVS.
OrthoDB	EOG4Z0FF0.
Gene expression databases
ArrayExpress	P17064.
Genevestigator	P17064.
GermOnline	YER056C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001248. Cyt_pur_permease. IPR012681. NCS1. [Graphical view]
Pfam	PF02133. Transp_cyt_pur. 1 hit. [Graphical view]
TIGRFAMs	TIGR00800. Ncs1. 1 hit.
ProtoNet	Search...
Other
NextBio	982997.

Entry information

Entry name

FCY2_YEAST

Accession

Primary (citable) accession number: P17064
Secondary accession number(s): D3DLV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents