P17063 (GIA1_GIAIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Giardin subunit alpha-1
Organism	Giardia intestinalis (Giardia lamblia)
Taxonomic identifier	5741 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Fornicata › Diplomonadida › Hexamitidae › Giardiinae › Giardia

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Giardins are involved in parasite attachment to the intestinal mucosa and in the cytoskeletal disassembly and reassembly that marks the transition from infectious trophozoite to transmissible cyst. They may interact with other cytoskeletal proteins such as microtubules in the microribbons or crossbridges, to maintain the integrity of the ventral disk.
Subcellular location	Cytoplasm › cytoskeleton. Note: Most likely in the edges of the ventral disk microribbons.
Sequence similarities	Belongs to the giardin subunit alpha family. Contains 1 annexin repeat.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Annexin
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	cytoskeleton organization Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 295

295

Giardin subunit alpha-1

PRO_0000067522

Regions

Repeat

4 – 69

66

Annexin

Secondary structure

1

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295

Helix Strand Turn

Details...

Helix

4 – 16

13

Helix

20 – 28

9

Helix

32 – 46

15

Helix

50 – 57

8

Helix

62 – 70

9

Helix

74 – 87

14

Helix

92 – 99

8

Helix

104 – 118

15

Helix

122 – 130

9

Beta strand

132 – 134

3

Helix

135 – 145

11

Helix

155 – 167

13

Helix

171 – 180

10

Helix

183 – 196

14

Beta strand

197 – 199

3

Helix

201 – 208

8

Helix

211 – 225

15

Helix

227 – 240

14

Helix

245 – 255

11

Helix

256 – 258

3

Helix

262 – 265

4

Helix

266 – 268

3

Helix

272 – 279

8

Helix

284 – 292

9

Sequences

	Sequence		Length	Mass (Da)
	P17063 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 9DA8F7318E7E4C50	FASTA	295	33,890
	10 20 30 40 50 60 MPKVTDIANE LKQAIDAKDE VQIAFIASEY SAESREKIAK AYVASYGKEL PDDIKKALKG 70 80 90 100 110 120 GSEESLLMDL FSDRHEVRAQ HIRDALSGRN DHMAFFDTVI LCTPEDWHET VAAYTRMFKK 130 140 150 160 170 180 PLVEDFMKDV GRKEDWCLLM EKWMAHERVS RPGSPEDEAQ RLDQAFDQKN TAYLIDFFGT 190 200 210 220 230 240 VPSAEYRPIA EAFKAQNGKS IEQAIATIYT KTDYYTFYCA HFALLGMHRL AAYLINCACN 250 260 270 280 290 DKGDEKRMRR ITGMMVDKCL GAKHAYKIYG DMGTDIERCF DKRMAPILRT LWRVK

References

[1]	"Ultrastructural localization of giardins to the edges of disk microribbons of Giarida lamblia and the nucleotide and deduced protein sequence of alpha giardin." Peattie D.A., Alonso R.A., Hein A., Caulfield J.P. J. Cell Biol. 109:2323-2335(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 3-31. Strain: Portland-1.
[2]	"Nucleotide sequence of a second alpha giardin gene and molecular analysis of the alpha giardin genes and transcripts in Giardia lamblia." Alonso R.A., Peattie D.A. Mol. Biochem. Parasitol. 50:95-104(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Portland-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X52485 Genomic DNA. Translation: CAA36727.1.

PIR

A33735.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4EVF	X-ray	1.90	A	1-295	[»]
4EVH	X-ray	2.60	A	1-295	[»]

ProteinModelPortal

P17063.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.220.10. 1 hit.

InterPro

IPR008088. Alpha_giardin.
IPR001464. Annexin.
IPR018502. Annexin_repeat.
[Graphical view]

Pfam

PF00191. Annexin. 1 hit.
[Graphical view]

PRINTS

PR01712. ALPHAGIARDIN.

SMART

SM00335. ANX. 1 hit.
[Graphical view]

SUPFAM

SSF47874. Annexin. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GIA1_GIAIN

Accession

Primary (citable) accession number: P17063

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)