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Protein

Phytoene synthase

Gene

crtB

Organism
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of carotenoids. Catalyzes the condensation of two molecules of geranylgeranyl diphosphate (GGPP) to give prephytoene diphosphate (PPPP) and the subsequent rearrangement of the cyclopropylcarbinyl intermediate to yield phytoene (By similarity).By similarity

Cofactori

Protein has several cofactor binding sites:
  • ATPBy similarityNote: ATP is required for the transferase activity but it does not seem to be hydrolyzed during the reaction.By similarity
  • Mn2+By similarity, Mg2+By similarity

Pathway: phytoene biosynthesis

This protein is involved in the pathway phytoene biosynthesis, which is part of Carotenoid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway phytoene biosynthesis and in Carotenoid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14934.
RCAP272942:GJIY-693-MONOMER.
UniPathwayiUPA00799.

Names & Taxonomyi

Protein namesi
Recommended name:
Phytoene synthase (EC:2.5.1.-)
Short name:
PSase
Gene namesi
Name:crtB
Ordered Locus Names:RCAP_rcc00680
OrganismiRhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Taxonomic identifieri272942 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
ProteomesiUP000002361 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Phytoene synthasePRO_0000067433Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272942.RCAP_rcc00680.

Structurei

3D structure databases

ProteinModelPortaliP17056.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phytoene/squalene synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000220848.
KOiK02291.
OMAiCAMQLTN.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17056-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAEADMEVC RELIRTGSYS FHAASRVLPA RVRDPALALY AFCRVADDEV
60 70 80 90 100
DEVGAPRDKA AAVLKLGDRL EDIYAGRPRN APSDRAFAAV VEEFEMPREL
110 120 130 140 150
PEALLEGFAW DAEGRWYHTL SDVQAYSARV AAAVGAMMCV LMRVRNPDAL
160 170 180 190 200
ARACDLGLAM QMSNIARDVG EDARAGRLFL PTDWMVEEGI DPQAFLADPQ
210 220 230 240 250
PTKGIRRVTE RLLNRADRLY WRAATGVRLL PFDCRPGIMA AGKIYAAIGA
260 270 280 290 300
EVAKAKYDNI TRRAHTTKGR KLWLVANSAM SATATSMLPL SPRVHAKPEP
310 320 330
EVAHLVDAAA HRNLHPERSE VLISALMALK ARDRGLAMD
Length:339
Mass (Da):37,302
Last modified:August 1, 1990 - v1
Checksum:i6D7052994934BF4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52291 Genomic DNA. Translation: CAA36534.1.
Z11165 Genomic DNA. Translation: CAA77541.1.
CP001312 Genomic DNA. Translation: ADE84445.1.
PIRiS04403.
RefSeqiWP_013066424.1. NC_014034.1.
YP_003576852.1. NC_014034.1.

Genome annotation databases

EnsemblBacteriaiADE84445; ADE84445; RCAP_rcc00680.
KEGGircp:RCAP_rcc00680.
PATRICi35501428. VBIRhoCap134200_0691.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52291 Genomic DNA. Translation: CAA36534.1.
Z11165 Genomic DNA. Translation: CAA77541.1.
CP001312 Genomic DNA. Translation: ADE84445.1.
PIRiS04403.
RefSeqiWP_013066424.1. NC_014034.1.
YP_003576852.1. NC_014034.1.

3D structure databases

ProteinModelPortaliP17056.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272942.RCAP_rcc00680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE84445; ADE84445; RCAP_rcc00680.
KEGGircp:RCAP_rcc00680.
PATRICi35501428. VBIRhoCap134200_0691.

Phylogenomic databases

HOGENOMiHOG000220848.
KOiK02291.
OMAiCAMQLTN.

Enzyme and pathway databases

UniPathwayiUPA00799.
BioCyciMetaCyc:MONOMER-14934.
RCAP272942:GJIY-693-MONOMER.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
PROSITEiPS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit.
PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus."
    Armstrong G.A., Alberti M., Leach F., Hearst J.E.
    Mol. Gen. Genet. 216:254-268(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-309 / NBRC 16581 / SB1003.
  2. "Complete genome sequence of the photosynthetic purple nonsulfur bacterium Rhodobacter capsulatus SB 1003."
    Strnad H., Lapidus A., Paces J., Ulbrich P., Vlcek C., Paces V., Haselkorn R.
    J. Bacteriol. 192:3545-3546(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-309 / NBRC 16581 / SB1003.

Entry informationi

Entry nameiCRTB_RHOCB
AccessioniPrimary (citable) accession number: P17056
Secondary accession number(s): D5AP73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 24, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.