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Protein

Lysosome-associated membrane glycoprotein 2

Gene

Lamp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.

GO - Molecular functioni

  • enzyme binding Source: MGI
  • protein domain specific binding Source: UniProtKB

GO - Biological processi

  • protein stabilization Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_307071. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome-associated membrane glycoprotein 2
Short name:
LAMP-2
Short name:
Lysosome-associated membrane protein 2
Alternative name(s):
CD107 antigen-like family member B
Lysosomal membrane glycoprotein type B
Short name:
LGP-B
CD_antigen: CD107b
Gene namesi
Name:Lamp2
Synonyms:Lamp-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:96748. Lamp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 379354LumenalSequence AnalysisAdd
BLAST
Transmembranei380 – 40425HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini405 – 41511CytoplasmicPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • late endosome Source: MGI
  • late endosome membrane Source: MGI
  • lysosomal membrane Source: BHF-UCL
  • lysosome Source: MGI
  • membrane Source: MGI
  • phagocytic vesicle membrane Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
  • platelet dense granule membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Add
BLAST
Chaini26 – 415390Lysosome-associated membrane glycoprotein 2PRO_0000017111Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi37 ↔ 75PROSITE-ProRule annotation
Glycosylationi45 – 451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi149 ↔ 185PROSITE-ProRule annotation
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi237 ↔ 270PROSITE-ProRule annotation
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi317 – 3171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi336 ↔ 373PROSITE-ProRule annotation
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

O- and N-glycosylated; some of the N-linked glycans are polylactosaminoglycans.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP17047.
PaxDbiP17047.
PRIDEiP17047.

PTM databases

PhosphoSiteiP17047.

Expressioni

Gene expression databases

BgeeiP17047.
CleanExiMM_LAMP2.
ExpressionAtlasiP17047. baseline and differential.
GenevisibleiP17047. MM.

Interactioni

Protein-protein interaction databases

BioGridi201106. 2 interactions.
IntActiP17047. 7 interactions.
MINTiMINT-1857994.
STRINGi10090.ENSMUSP00000016678.

Structurei

3D structure databases

ProteinModelPortaliP17047.
SMRiP17047. Positions 226-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni26 – 188163First lumenal domainAdd
BLAST
Regioni189 – 23345HingeAdd
BLAST
Regioni234 – 379146Second lumenal domainAdd
BLAST

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG301998.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiP17047.
KOiK06528.
OrthoDBiEOG7ZD1VH.
TreeFamiTF316339.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform LAMP-2A (identifier: P17047-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCLSPVKGAK LILIFLFLGA VQSNALIVNL TDSKGTCLYA EWEMNFTITY
60 70 80 90 100
ETTNQTNKTI TIAVPDKATH DGSSCGDDRN SAKIMIQFGF AVSWAVNFTK
110 120 130 140 150
EASHYSIHDI VLSYNTSDST VFPGAVAKGV HTVKNPENFK VPLDVIFKCN
160 170 180 190 200
SVLTYNLTPV VQKYWGIHLQ AFVQNGTVSK NEQVCEEDQT PTTVAPIIHT
210 220 230 240 250
TAPSTTTTLT PTSTPTPTPT PTPTVGNYSI RNGNTTCLLA TMGLQLNITE
260 270 280 290 300
EKVPFIFNIN PATTNFTGSC QPQSAQLRLN NSQIKYLDFI FAVKNEKRFY
310 320 330 340 350
LKEVNVYMYL ANGSAFNISN KNLSFWDAPL GSSYMCNKEQ VLSVSRAFQI
360 370 380 390 400
NTFNLKVQPF NVTKGQYSTA QDCSADEDNF LVPIAVGAAL GGVLILVLLA
410
YFIGLKRHHT GYEQF
Length:415
Mass (Da):45,681
Last modified:May 31, 2011 - v2
Checksum:i053A874B4DF67C88
GO
Isoform LAMP-2B (identifier: P17047-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     371-415: QDCSADEDNF...KRHHTGYEQF → EECAADSDLN...RKSRTGYQSV

Show »
Length:416
Mass (Da):45,797
Checksum:iF6FE0E2F2B5689E2
GO
Isoform LAMP-2C (identifier: P17047-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     372-415: DCSADEDNFL...KRHHTGYEQF → ECSLDDDTIL...RKTYAGYQTL

Show »
Length:415
Mass (Da):45,655
Checksum:iFF0021CBFDEED589
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti272 – 2721P → H in BAE34951 (PubMed:16141072).Curated
Sequence conflicti289 – 2891F → L in AAA39412 (PubMed:2318880).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei371 – 41545QDCSA…GYEQF → EECAADSDLNFLIPVAVGVA LGFLIIAVFISYMIGRRKSR TGYQSV in isoform LAMP-2B. 2 PublicationsVSP_003045Add
BLAST
Alternative sequencei372 – 41544DCSAD…GYEQF → ECSLDDDTILIPIIVGAGLS GLIIVIVIAYLIGRRKTYAG YQTL in isoform LAMP-2C. CuratedVSP_003046Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05287 mRNA. Translation: AAA39412.1.
AK159272 mRNA. Translation: BAE34951.1.
AK032974 mRNA. Translation: BAC28106.1.
AK163933 mRNA. Translation: BAE37543.1.
AL513356 Genomic DNA. Translation: CAM17143.1.
AL513356 Genomic DNA. Translation: CAM17144.1.
CH466570 Genomic DNA. Translation: EDL29016.1.
CH466570 Genomic DNA. Translation: EDL29018.1.
BC138718 mRNA. Translation: AAI38719.1.
BC138723 mRNA. Translation: AAI38724.1.
M32017 mRNA. Translation: AAA39429.1.
M32018 Genomic DNA. Translation: AAA39430.1.
CCDSiCCDS30092.1. [P17047-1]
CCDS30093.1. [P17047-3]
CCDS72369.1. [P17047-2]
PIRiA35560.
RefSeqiNP_001017959.1. NM_001017959.2. [P17047-1]
NP_001277414.1. NM_001290485.1. [P17047-2]
NP_034815.2. NM_010685.4. [P17047-3]
UniGeneiMm.414016.
Mm.486.

Genome annotation databases

EnsembliENSMUST00000016678; ENSMUSP00000016678; ENSMUSG00000016534. [P17047-1]
ENSMUST00000061755; ENSMUSP00000052283; ENSMUSG00000016534. [P17047-3]
ENSMUST00000074913; ENSMUSP00000074448; ENSMUSG00000016534. [P17047-2]
GeneIDi16784.
KEGGimmu:16784.
UCSCiuc009szw.1. mouse. [P17047-1]
uc009szx.1. mouse. [P17047-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05287 mRNA. Translation: AAA39412.1.
AK159272 mRNA. Translation: BAE34951.1.
AK032974 mRNA. Translation: BAC28106.1.
AK163933 mRNA. Translation: BAE37543.1.
AL513356 Genomic DNA. Translation: CAM17143.1.
AL513356 Genomic DNA. Translation: CAM17144.1.
CH466570 Genomic DNA. Translation: EDL29016.1.
CH466570 Genomic DNA. Translation: EDL29018.1.
BC138718 mRNA. Translation: AAI38719.1.
BC138723 mRNA. Translation: AAI38724.1.
M32017 mRNA. Translation: AAA39429.1.
M32018 Genomic DNA. Translation: AAA39430.1.
CCDSiCCDS30092.1. [P17047-1]
CCDS30093.1. [P17047-3]
CCDS72369.1. [P17047-2]
PIRiA35560.
RefSeqiNP_001017959.1. NM_001017959.2. [P17047-1]
NP_001277414.1. NM_001290485.1. [P17047-2]
NP_034815.2. NM_010685.4. [P17047-3]
UniGeneiMm.414016.
Mm.486.

3D structure databases

ProteinModelPortaliP17047.
SMRiP17047. Positions 226-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201106. 2 interactions.
IntActiP17047. 7 interactions.
MINTiMINT-1857994.
STRINGi10090.ENSMUSP00000016678.

PTM databases

PhosphoSiteiP17047.

Proteomic databases

MaxQBiP17047.
PaxDbiP17047.
PRIDEiP17047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016678; ENSMUSP00000016678; ENSMUSG00000016534. [P17047-1]
ENSMUST00000061755; ENSMUSP00000052283; ENSMUSG00000016534. [P17047-3]
ENSMUST00000074913; ENSMUSP00000074448; ENSMUSG00000016534. [P17047-2]
GeneIDi16784.
KEGGimmu:16784.
UCSCiuc009szw.1. mouse. [P17047-1]
uc009szx.1. mouse. [P17047-3]

Organism-specific databases

CTDi3920.
MGIiMGI:96748. Lamp2.

Phylogenomic databases

eggNOGiNOG301998.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiP17047.
KOiK06528.
OrthoDBiEOG7ZD1VH.
TreeFamiTF316339.

Enzyme and pathway databases

ReactomeiREACT_307071. Platelet degranulation.

Miscellaneous databases

ChiTaRSiLamp2. mouse.
NextBioi290644.
PROiP17047.
SOURCEiSearch...

Gene expression databases

BgeeiP17047.
CleanExiMM_LAMP2.
ExpressionAtlasiP17047. baseline and differential.
GenevisibleiP17047. MM.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins."
    Cha Y., Holland S.M., August J.T.
    J. Biol. Chem. 265:5008-5013(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LAMP-2A).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LAMP-2A AND LAMP-2B).
    Strain: C57BL/6J.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LAMP-2B).
  6. "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells."
    Granger B.L., Green S.A., Gabel C.A., Howe C.L., Mellman I., Helenius A.
    J. Biol. Chem. 265:12036-12043(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 58-128 AND 186-415.
  7. "The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c."
    Gough N.R., Hatem C.L., Fambrough D.M.
    DNA Cell Biol. 14:863-867(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING.
    Tissue: Brain.

Entry informationi

Entry nameiLAMP2_MOUSE
AccessioniPrimary (citable) accession number: P17047
Secondary accession number(s): A2A430, Q3TXG5, Q8BSG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 31, 2011
Last modified: June 24, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.