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P17047

- LAMP2_MOUSE

UniProt

P17047 - LAMP2_MOUSE

Protein

Lysosome-associated membrane glycoprotein 2

Gene

Lamp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (31 May 2011)
      Previous versions | rss
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    Functioni

    Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.

    GO - Molecular functioni

    1. protein domain specific binding Source: UniProt

    GO - Biological processi

    1. protein stabilization Source: UniProt

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysosome-associated membrane glycoprotein 2
    Short name:
    LAMP-2
    Short name:
    Lysosome-associated membrane protein 2
    Alternative name(s):
    CD107 antigen-like family member B
    Lysosomal membrane glycoprotein type B
    Short name:
    LGP-B
    CD_antigen: CD107b
    Gene namesi
    Name:Lamp2
    Synonyms:Lamp-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:96748. Lamp2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. late endosome Source: MGI
    3. late endosome membrane Source: MGI
    4. lysosomal membrane Source: BHF-UCL
    5. lysosome Source: MGI
    6. membrane Source: MGI
    7. phagocytic vesicle membrane Source: MGI
    8. plasma membrane Source: UniProtKB-SubCell
    9. platelet dense granule membrane Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Endosome, Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Add
    BLAST
    Chaini26 – 415390Lysosome-associated membrane glycoprotein 2PRO_0000017111Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi37 ↔ 75PROSITE-ProRule annotation
    Glycosylationi45 – 451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi149 ↔ 185PROSITE-ProRule annotation
    Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi237 ↔ 270PROSITE-ProRule annotation
    Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi317 – 3171N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi336 ↔ 373PROSITE-ProRule annotation
    Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    O- and N-glycosylated; some of the N-linked glycans are polylactosaminoglycans.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP17047.
    PaxDbiP17047.
    PRIDEiP17047.

    PTM databases

    PhosphoSiteiP17047.

    Expressioni

    Gene expression databases

    ArrayExpressiP17047.
    BgeeiP17047.
    CleanExiMM_LAMP2.
    GenevestigatoriP17047.

    Interactioni

    Protein-protein interaction databases

    BioGridi201106. 2 interactions.
    IntActiP17047. 7 interactions.
    MINTiMINT-1857994.
    STRINGi10090.ENSMUSP00000074448.

    Structurei

    3D structure databases

    ProteinModelPortaliP17047.
    SMRiP17047. Positions 226-375.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini26 – 379354LumenalSequence AnalysisAdd
    BLAST
    Topological domaini405 – 41511CytoplasmicPROSITE-ProRule annotationAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei380 – 40425HelicalPROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni26 – 188163First lumenal domainAdd
    BLAST
    Regioni189 – 23345HingeAdd
    BLAST
    Regioni234 – 379146Second lumenal domainAdd
    BLAST

    Sequence similaritiesi

    Belongs to the LAMP family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG301998.
    GeneTreeiENSGT00530000063068.
    HOGENOMiHOG000230942.
    HOVERGENiHBG052303.
    KOiK06528.
    OMAiAPNESEQ.
    OrthoDBiEOG7ZD1VH.
    TreeFamiTF316339.

    Family and domain databases

    InterProiIPR018134. LAMP_CS.
    IPR002000. Lysosome-assoc_membr_glycop.
    [Graphical view]
    PANTHERiPTHR11506. PTHR11506. 1 hit.
    PfamiPF01299. Lamp. 1 hit.
    [Graphical view]
    PRINTSiPR00336. LYSASSOCTDMP.
    PROSITEiPS00310. LAMP_1. 1 hit.
    PS00311. LAMP_2. 1 hit.
    PS51407. LAMP_3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform LAMP-2A (identifier: P17047-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCLSPVKGAK LILIFLFLGA VQSNALIVNL TDSKGTCLYA EWEMNFTITY    50
    ETTNQTNKTI TIAVPDKATH DGSSCGDDRN SAKIMIQFGF AVSWAVNFTK 100
    EASHYSIHDI VLSYNTSDST VFPGAVAKGV HTVKNPENFK VPLDVIFKCN 150
    SVLTYNLTPV VQKYWGIHLQ AFVQNGTVSK NEQVCEEDQT PTTVAPIIHT 200
    TAPSTTTTLT PTSTPTPTPT PTPTVGNYSI RNGNTTCLLA TMGLQLNITE 250
    EKVPFIFNIN PATTNFTGSC QPQSAQLRLN NSQIKYLDFI FAVKNEKRFY 300
    LKEVNVYMYL ANGSAFNISN KNLSFWDAPL GSSYMCNKEQ VLSVSRAFQI 350
    NTFNLKVQPF NVTKGQYSTA QDCSADEDNF LVPIAVGAAL GGVLILVLLA 400
    YFIGLKRHHT GYEQF 415
    Length:415
    Mass (Da):45,681
    Last modified:May 31, 2011 - v2
    Checksum:i053A874B4DF67C88
    GO
    Isoform LAMP-2B (identifier: P17047-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         371-415: QDCSADEDNF...KRHHTGYEQF → EECAADSDLN...RKSRTGYQSV

    Show »
    Length:416
    Mass (Da):45,797
    Checksum:iF6FE0E2F2B5689E2
    GO
    Isoform LAMP-2C (identifier: P17047-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         372-415: DCSADEDNFL...KRHHTGYEQF → ECSLDDDTIL...RKTYAGYQTL

    Show »
    Length:415
    Mass (Da):45,655
    Checksum:iFF0021CBFDEED589
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti272 – 2721P → H in BAE34951. (PubMed:16141072)Curated
    Sequence conflicti289 – 2891F → L in AAA39412. (PubMed:2318880)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei371 – 41545QDCSA…GYEQF → EECAADSDLNFLIPVAVGVA LGFLIIAVFISYMIGRRKSR TGYQSV in isoform LAMP-2B. 2 PublicationsVSP_003045Add
    BLAST
    Alternative sequencei372 – 41544DCSAD…GYEQF → ECSLDDDTILIPIIVGAGLS GLIIVIVIAYLIGRRKTYAG YQTL in isoform LAMP-2C. CuratedVSP_003046Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05287 mRNA. Translation: AAA39412.1.
    AK159272 mRNA. Translation: BAE34951.1.
    AK032974 mRNA. Translation: BAC28106.1.
    AK163933 mRNA. Translation: BAE37543.1.
    AL513356 Genomic DNA. Translation: CAM17143.1.
    AL513356 Genomic DNA. Translation: CAM17144.1.
    CH466570 Genomic DNA. Translation: EDL29016.1.
    CH466570 Genomic DNA. Translation: EDL29018.1.
    BC138718 mRNA. Translation: AAI38719.1.
    BC138723 mRNA. Translation: AAI38724.1.
    M32017 mRNA. Translation: AAA39429.1.
    M32018 Genomic DNA. Translation: AAA39430.1.
    CCDSiCCDS30092.1. [P17047-1]
    CCDS30093.1. [P17047-3]
    CCDS72369.1. [P17047-2]
    PIRiA35560.
    RefSeqiNP_001017959.1. NM_001017959.2. [P17047-1]
    NP_001277414.1. NM_001290485.1. [P17047-2]
    NP_034815.2. NM_010685.4. [P17047-3]
    UniGeneiMm.414016.
    Mm.486.

    Genome annotation databases

    EnsembliENSMUST00000016678; ENSMUSP00000016678; ENSMUSG00000016534. [P17047-1]
    ENSMUST00000061755; ENSMUSP00000052283; ENSMUSG00000016534. [P17047-3]
    ENSMUST00000074913; ENSMUSP00000074448; ENSMUSG00000016534. [P17047-2]
    GeneIDi16784.
    KEGGimmu:16784.
    UCSCiuc009szw.1. mouse. [P17047-1]
    uc009szx.1. mouse. [P17047-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05287 mRNA. Translation: AAA39412.1 .
    AK159272 mRNA. Translation: BAE34951.1 .
    AK032974 mRNA. Translation: BAC28106.1 .
    AK163933 mRNA. Translation: BAE37543.1 .
    AL513356 Genomic DNA. Translation: CAM17143.1 .
    AL513356 Genomic DNA. Translation: CAM17144.1 .
    CH466570 Genomic DNA. Translation: EDL29016.1 .
    CH466570 Genomic DNA. Translation: EDL29018.1 .
    BC138718 mRNA. Translation: AAI38719.1 .
    BC138723 mRNA. Translation: AAI38724.1 .
    M32017 mRNA. Translation: AAA39429.1 .
    M32018 Genomic DNA. Translation: AAA39430.1 .
    CCDSi CCDS30092.1. [P17047-1 ]
    CCDS30093.1. [P17047-3 ]
    CCDS72369.1. [P17047-2 ]
    PIRi A35560.
    RefSeqi NP_001017959.1. NM_001017959.2. [P17047-1 ]
    NP_001277414.1. NM_001290485.1. [P17047-2 ]
    NP_034815.2. NM_010685.4. [P17047-3 ]
    UniGenei Mm.414016.
    Mm.486.

    3D structure databases

    ProteinModelPortali P17047.
    SMRi P17047. Positions 226-375.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201106. 2 interactions.
    IntActi P17047. 7 interactions.
    MINTi MINT-1857994.
    STRINGi 10090.ENSMUSP00000074448.

    PTM databases

    PhosphoSitei P17047.

    Proteomic databases

    MaxQBi P17047.
    PaxDbi P17047.
    PRIDEi P17047.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000016678 ; ENSMUSP00000016678 ; ENSMUSG00000016534 . [P17047-1 ]
    ENSMUST00000061755 ; ENSMUSP00000052283 ; ENSMUSG00000016534 . [P17047-3 ]
    ENSMUST00000074913 ; ENSMUSP00000074448 ; ENSMUSG00000016534 . [P17047-2 ]
    GeneIDi 16784.
    KEGGi mmu:16784.
    UCSCi uc009szw.1. mouse. [P17047-1 ]
    uc009szx.1. mouse. [P17047-3 ]

    Organism-specific databases

    CTDi 3920.
    MGIi MGI:96748. Lamp2.

    Phylogenomic databases

    eggNOGi NOG301998.
    GeneTreei ENSGT00530000063068.
    HOGENOMi HOG000230942.
    HOVERGENi HBG052303.
    KOi K06528.
    OMAi APNESEQ.
    OrthoDBi EOG7ZD1VH.
    TreeFami TF316339.

    Miscellaneous databases

    ChiTaRSi LAMP2. mouse.
    NextBioi 290644.
    PROi P17047.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P17047.
    Bgeei P17047.
    CleanExi MM_LAMP2.
    Genevestigatori P17047.

    Family and domain databases

    InterProi IPR018134. LAMP_CS.
    IPR002000. Lysosome-assoc_membr_glycop.
    [Graphical view ]
    PANTHERi PTHR11506. PTHR11506. 1 hit.
    Pfami PF01299. Lamp. 1 hit.
    [Graphical view ]
    PRINTSi PR00336. LYSASSOCTDMP.
    PROSITEi PS00310. LAMP_1. 1 hit.
    PS00311. LAMP_2. 1 hit.
    PS51407. LAMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins."
      Cha Y., Holland S.M., August J.T.
      J. Biol. Chem. 265:5008-5013(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LAMP-2A).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS LAMP-2A AND LAMP-2B).
      Strain: C57BL/6J.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LAMP-2B).
    6. "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells."
      Granger B.L., Green S.A., Gabel C.A., Howe C.L., Mellman I., Helenius A.
      J. Biol. Chem. 265:12036-12043(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 58-128 AND 186-415.
    7. "The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c."
      Gough N.R., Hatem C.L., Fambrough D.M.
      DNA Cell Biol. 14:863-867(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
      Tissue: Brain.

    Entry informationi

    Entry nameiLAMP2_MOUSE
    AccessioniPrimary (citable) accession number: P17047
    Secondary accession number(s): A2A430, Q3TXG5, Q8BSG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: May 31, 2011
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3