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Protein

Lysosome-associated membrane glycoprotein 2

Gene

Lamp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter- and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens.

GO - Molecular functioni

  • enzyme binding Source: MGI
  • protein domain specific binding Source: UniProtKB

GO - Biological processi

  • chaperone-mediated autophagy Source: ParkinsonsUK-UCL
  • muscle cell cellular homeostasis Source: MGI
  • protein stabilization Source: UniProtKB
  • protein targeting to lysosome involved in chaperone-mediated autophagy Source: MGI
  • regulation of protein stability Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome-associated membrane glycoprotein 2
Short name:
LAMP-2
Short name:
Lysosome-associated membrane protein 2
Alternative name(s):
CD107 antigen-like family member B
Lysosomal membrane glycoprotein type B
Short name:
LGP-B
CD_antigen: CD107b
Gene namesi
Name:Lamp2
Synonyms:Lamp-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:96748. Lamp2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 379LumenalSequence analysisAdd BLAST354
Transmembranei380 – 404HelicalPROSITE-ProRule annotationAdd BLAST25
Topological domaini405 – 415CytoplasmicPROSITE-ProRule annotationAdd BLAST11

GO - Cellular componenti

  • autolysosome Source: MGI
  • chaperone-mediated autophagy translocation complex Source: ParkinsonsUK-UCL
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • late endosome Source: MGI
  • late endosome membrane Source: MGI
  • lysosomal matrix Source: MGI
  • lysosomal membrane Source: BHF-UCL
  • lysosome Source: MGI
  • membrane Source: MGI
  • phagocytic vesicle membrane Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
  • platelet dense granule membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
ChainiPRO_000001711126 – 415Lysosome-associated membrane glycoprotein 2Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi37 ↔ 75PROSITE-ProRule annotation
Glycosylationi45N-linked (GlcNAc...)Sequence analysis1
Glycosylationi54N-linked (GlcNAc...)Sequence analysis1
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Glycosylationi115N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi149 ↔ 185PROSITE-ProRule annotation
Glycosylationi175N-linked (GlcNAc...)Sequence analysis1
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi234N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi237 ↔ 270PROSITE-ProRule annotation
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1
Glycosylationi280N-linked (GlcNAc...)Sequence analysis1
Glycosylationi312N-linked (GlcNAc...)Sequence analysis1
Glycosylationi317N-linked (GlcNAc...)Sequence analysis1
Glycosylationi322N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi336 ↔ 373PROSITE-ProRule annotation
Glycosylationi361N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

O- and N-glycosylated; some of the N-linked glycans are polylactosaminoglycans.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP17047.
PaxDbiP17047.
PeptideAtlasiP17047.
PRIDEiP17047.

PTM databases

iPTMnetiP17047.
PhosphoSitePlusiP17047.
SwissPalmiP17047.

Expressioni

Gene expression databases

BgeeiENSMUSG00000016534.
CleanExiMM_LAMP2.
ExpressionAtlasiP17047. baseline and differential.
GenevisibleiP17047. MM.

Interactioni

Subunit structurei

Interacts with MLLT11.By similarity

GO - Molecular functioni

  • enzyme binding Source: MGI
  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi201106. 3 interactors.
IntActiP17047. 7 interactors.
MINTiMINT-1857994.
STRINGi10090.ENSMUSP00000016678.

Structurei

3D structure databases

ProteinModelPortaliP17047.
SMRiP17047.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 188First lumenal domainAdd BLAST163
Regioni189 – 233HingeAdd BLAST45
Regioni234 – 379Second lumenal domainAdd BLAST146

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiP17047.
KOiK06528.
OMAiAEECFAD.
TreeFamiTF316339.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform LAMP-2A (identifier: P17047-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCLSPVKGAK LILIFLFLGA VQSNALIVNL TDSKGTCLYA EWEMNFTITY
60 70 80 90 100
ETTNQTNKTI TIAVPDKATH DGSSCGDDRN SAKIMIQFGF AVSWAVNFTK
110 120 130 140 150
EASHYSIHDI VLSYNTSDST VFPGAVAKGV HTVKNPENFK VPLDVIFKCN
160 170 180 190 200
SVLTYNLTPV VQKYWGIHLQ AFVQNGTVSK NEQVCEEDQT PTTVAPIIHT
210 220 230 240 250
TAPSTTTTLT PTSTPTPTPT PTPTVGNYSI RNGNTTCLLA TMGLQLNITE
260 270 280 290 300
EKVPFIFNIN PATTNFTGSC QPQSAQLRLN NSQIKYLDFI FAVKNEKRFY
310 320 330 340 350
LKEVNVYMYL ANGSAFNISN KNLSFWDAPL GSSYMCNKEQ VLSVSRAFQI
360 370 380 390 400
NTFNLKVQPF NVTKGQYSTA QDCSADEDNF LVPIAVGAAL GGVLILVLLA
410
YFIGLKRHHT GYEQF
Length:415
Mass (Da):45,681
Last modified:May 31, 2011 - v2
Checksum:i053A874B4DF67C88
GO
Isoform LAMP-2B (identifier: P17047-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     371-415: QDCSADEDNF...KRHHTGYEQF → EECAADSDLN...RKSRTGYQSV

Show »
Length:416
Mass (Da):45,797
Checksum:iF6FE0E2F2B5689E2
GO
Isoform LAMP-2C (identifier: P17047-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     372-415: DCSADEDNFL...KRHHTGYEQF → ECSLDDDTIL...RKTYAGYQTL

Show »
Length:415
Mass (Da):45,655
Checksum:iFF0021CBFDEED589
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272P → H in BAE34951 (PubMed:16141072).Curated1
Sequence conflicti289F → L in AAA39412 (PubMed:2318880).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003045371 – 415QDCSA…GYEQF → EECAADSDLNFLIPVAVGVA LGFLIIAVFISYMIGRRKSR TGYQSV in isoform LAMP-2B. 2 PublicationsAdd BLAST45
Alternative sequenceiVSP_003046372 – 415DCSAD…GYEQF → ECSLDDDTILIPIIVGAGLS GLIIVIVIAYLIGRRKTYAG YQTL in isoform LAMP-2C. CuratedAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05287 mRNA. Translation: AAA39412.1.
AK159272 mRNA. Translation: BAE34951.1.
AK032974 mRNA. Translation: BAC28106.1.
AK163933 mRNA. Translation: BAE37543.1.
AL513356 Genomic DNA. Translation: CAM17143.1.
AL513356 Genomic DNA. Translation: CAM17144.1.
CH466570 Genomic DNA. Translation: EDL29016.1.
CH466570 Genomic DNA. Translation: EDL29018.1.
BC138718 mRNA. Translation: AAI38719.1.
BC138723 mRNA. Translation: AAI38724.1.
M32017 mRNA. Translation: AAA39429.1.
M32018 Genomic DNA. Translation: AAA39430.1.
CCDSiCCDS30092.1. [P17047-1]
CCDS30093.1. [P17047-3]
CCDS72369.1. [P17047-2]
PIRiA35560.
RefSeqiNP_001017959.1. NM_001017959.2. [P17047-1]
NP_001277414.1. NM_001290485.1. [P17047-2]
NP_034815.2. NM_010685.4. [P17047-3]
UniGeneiMm.414016.
Mm.486.

Genome annotation databases

EnsembliENSMUST00000016678; ENSMUSP00000016678; ENSMUSG00000016534. [P17047-1]
ENSMUST00000061755; ENSMUSP00000052283; ENSMUSG00000016534. [P17047-3]
ENSMUST00000074913; ENSMUSP00000074448; ENSMUSG00000016534. [P17047-2]
GeneIDi16784.
KEGGimmu:16784.
UCSCiuc009szw.2. mouse. [P17047-1]
uc009szx.2. mouse. [P17047-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05287 mRNA. Translation: AAA39412.1.
AK159272 mRNA. Translation: BAE34951.1.
AK032974 mRNA. Translation: BAC28106.1.
AK163933 mRNA. Translation: BAE37543.1.
AL513356 Genomic DNA. Translation: CAM17143.1.
AL513356 Genomic DNA. Translation: CAM17144.1.
CH466570 Genomic DNA. Translation: EDL29016.1.
CH466570 Genomic DNA. Translation: EDL29018.1.
BC138718 mRNA. Translation: AAI38719.1.
BC138723 mRNA. Translation: AAI38724.1.
M32017 mRNA. Translation: AAA39429.1.
M32018 Genomic DNA. Translation: AAA39430.1.
CCDSiCCDS30092.1. [P17047-1]
CCDS30093.1. [P17047-3]
CCDS72369.1. [P17047-2]
PIRiA35560.
RefSeqiNP_001017959.1. NM_001017959.2. [P17047-1]
NP_001277414.1. NM_001290485.1. [P17047-2]
NP_034815.2. NM_010685.4. [P17047-3]
UniGeneiMm.414016.
Mm.486.

3D structure databases

ProteinModelPortaliP17047.
SMRiP17047.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201106. 3 interactors.
IntActiP17047. 7 interactors.
MINTiMINT-1857994.
STRINGi10090.ENSMUSP00000016678.

PTM databases

iPTMnetiP17047.
PhosphoSitePlusiP17047.
SwissPalmiP17047.

Proteomic databases

MaxQBiP17047.
PaxDbiP17047.
PeptideAtlasiP17047.
PRIDEiP17047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016678; ENSMUSP00000016678; ENSMUSG00000016534. [P17047-1]
ENSMUST00000061755; ENSMUSP00000052283; ENSMUSG00000016534. [P17047-3]
ENSMUST00000074913; ENSMUSP00000074448; ENSMUSG00000016534. [P17047-2]
GeneIDi16784.
KEGGimmu:16784.
UCSCiuc009szw.2. mouse. [P17047-1]
uc009szx.2. mouse. [P17047-3]

Organism-specific databases

CTDi3920.
MGIiMGI:96748. Lamp2.

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiP17047.
KOiK06528.
OMAiAEECFAD.
TreeFamiTF316339.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiLamp2. mouse.
PROiP17047.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016534.
CleanExiMM_LAMP2.
ExpressionAtlasiP17047. baseline and differential.
GenevisibleiP17047. MM.

Family and domain databases

InterProiIPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
[Graphical view]
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiPF01299. Lamp. 1 hit.
[Graphical view]
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiPS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLAMP2_MOUSE
AccessioniPrimary (citable) accession number: P17047
Secondary accession number(s): A2A430, Q3TXG5, Q8BSG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 31, 2011
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.