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Protein

Lysosome-associated membrane glycoprotein 2

Gene

Lamp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:10972293). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032). Required for normal degradation of the contents of autophagosomes (PubMed:10972293, PubMed:12221139). Plays a role in lysosomal protein degradation in response to starvation (PubMed:27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity).By similarity3 Publications

GO - Molecular functioni

  • enzyme binding Source: MGI
  • protein domain specific binding Source: UniProtKB

GO - Biological processi

  • autophagosome maturation Source: UniProtKB
  • autophagy Source: UniProtKB
  • cellular response to starvation Source: UniProtKB
  • chaperone-mediated autophagy Source: UniProtKB
  • lysosomal protein catabolic process Source: UniProtKB
  • muscle cell cellular homeostasis Source: MGI
  • negative regulation of protein homooligomerization Source: MGI
  • protein stabilization Source: UniProtKB
  • protein targeting Source: UniProtKB
  • protein targeting to lysosome involved in chaperone-mediated autophagy Source: UniProtKB
  • regulation of protein stability Source: MGI

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome-associated membrane glycoprotein 2
Short name:
LAMP-2
Short name:
Lysosome-associated membrane protein 2
Alternative name(s):
CD107 antigen-like family member B
Lysosomal membrane glycoprotein type B
Short name:
LGP-B
CD_antigen: CD107b
Gene namesi
Name:Lamp2
Synonyms:Lamp-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:96748. Lamp2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 379LumenalSequence analysisAdd BLAST354
Transmembranei380 – 404HelicalPROSITE-ProRule annotationAdd BLAST25
Topological domaini405 – 415CytoplasmicPROSITE-ProRule annotationAdd BLAST11

GO - Cellular componenti

  • autolysosome Source: MGI
  • chaperone-mediated autophagy translocation complex Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • integral component of autophagosome membrane Source: UniProtKB
  • late endosome Source: MGI
  • late endosome membrane Source: MGI
  • lysosomal matrix Source: MGI
  • lysosomal membrane Source: BHF-UCL
  • lysosome Source: MGI
  • membrane Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • phagocytic vesicle membrane Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
  • platelet dense granule membrane Source: MGI
  • trans-Golgi network Source: MGI

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

About half of the mutant mice die between 20 and 40 days after birth. Survivors are smaller, weigh 10 to 15 % less than their littermates, but show normal lifespan. Both mice that die early and long-time survivors display an accumulation of autophagic vacuoles in liver, pancreas, cardiac and skeletal muscle. Mutant mice display an increased ratio of heart weight to body weight and severely impaired contractile function of the heart muscle. Hepatocytes from mutant mice show a decreased rate of degradation of long-lived proteins.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
ChainiPRO_000001711126 – 415Lysosome-associated membrane glycoprotein 2Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi37 ↔ 75PROSITE-ProRule annotation
Glycosylationi45N-linked (GlcNAc...)Sequence analysis1
Glycosylationi54N-linked (GlcNAc...)Sequence analysis1
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Glycosylationi115N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi149 ↔ 185PROSITE-ProRule annotation
Glycosylationi175N-linked (GlcNAc...)Sequence analysis1
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi234N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi237 ↔ 270PROSITE-ProRule annotation
Glycosylationi247N-linked (GlcNAc...)Sequence analysis1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1
Glycosylationi280N-linked (GlcNAc...)Sequence analysis1
Glycosylationi312N-linked (GlcNAc...)Sequence analysis1
Glycosylationi317N-linked (GlcNAc...)Sequence analysis1
Glycosylationi322N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi336 ↔ 373PROSITE-ProRule annotation
Glycosylationi361N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Extensively N-glycosylated. Contains a minor proportion of O-linked glycans.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP17047.
PaxDbiP17047.
PeptideAtlasiP17047.
PRIDEiP17047.

PTM databases

iPTMnetiP17047.
PhosphoSitePlusiP17047.
SwissPalmiP17047.

Expressioni

Tissue specificityi

Detected in liver and kidney (at protein level). Detected in liver and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000016534.
CleanExiMM_LAMP2.
ExpressionAtlasiP17047. baseline and differential.
GenevisibleiP17047. MM.

Interactioni

Subunit structurei

Monomer. Forms large homooligomers. Interacts (via its cytoplasmic region) with HSPA8. Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (By similarity). Interacts with MLLT11 (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: MGI
  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi201106. 3 interactors.
IntActiP17047. 7 interactors.
MINTiMINT-1857994.
STRINGi10090.ENSMUSP00000016678.

Structurei

3D structure databases

ProteinModelPortaliP17047.
SMRiP17047.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 188First lumenal domainAdd BLAST163
Regioni189 – 233HingeAdd BLAST45
Regioni234 – 379Second lumenal domainAdd BLAST146
Regioni406 – 409Important for binding and subsequent lysosomal degradation of target proteinsBy similarity4

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiP17047.
KOiK06528.
OMAiAEECFAD.
TreeFamiTF316339.

Family and domain databases

InterProiView protein in InterPro
IPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiView protein in Pfam
PF01299. Lamp. 1 hit.
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiView protein in PROSITE
PS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform LAMP-2A (identifier: P17047-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCLSPVKGAK LILIFLFLGA VQSNALIVNL TDSKGTCLYA EWEMNFTITY
60 70 80 90 100
ETTNQTNKTI TIAVPDKATH DGSSCGDDRN SAKIMIQFGF AVSWAVNFTK
110 120 130 140 150
EASHYSIHDI VLSYNTSDST VFPGAVAKGV HTVKNPENFK VPLDVIFKCN
160 170 180 190 200
SVLTYNLTPV VQKYWGIHLQ AFVQNGTVSK NEQVCEEDQT PTTVAPIIHT
210 220 230 240 250
TAPSTTTTLT PTSTPTPTPT PTPTVGNYSI RNGNTTCLLA TMGLQLNITE
260 270 280 290 300
EKVPFIFNIN PATTNFTGSC QPQSAQLRLN NSQIKYLDFI FAVKNEKRFY
310 320 330 340 350
LKEVNVYMYL ANGSAFNISN KNLSFWDAPL GSSYMCNKEQ VLSVSRAFQI
360 370 380 390 400
NTFNLKVQPF NVTKGQYSTA QDCSADEDNF LVPIAVGAAL GGVLILVLLA
410
YFIGLKRHHT GYEQF
Length:415
Mass (Da):45,681
Last modified:May 31, 2011 - v2
Checksum:i053A874B4DF67C88
GO
Isoform LAMP-2B (identifier: P17047-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     371-415: QDCSADEDNF...KRHHTGYEQF → EECAADSDLN...RKSRTGYQSV

Show »
Length:416
Mass (Da):45,797
Checksum:iF6FE0E2F2B5689E2
GO
Isoform LAMP-2C (identifier: P17047-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     372-415: DCSADEDNFL...KRHHTGYEQF → ECSLDDDTIL...RKTYAGYQTL

Show »
Length:415
Mass (Da):45,655
Checksum:iFF0021CBFDEED589
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272P → H in BAE34951 (PubMed:16141072).Curated1
Sequence conflicti289F → L in AAA39412 (PubMed:2318880).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003045371 – 415QDCSA…GYEQF → EECAADSDLNFLIPVAVGVA LGFLIIAVFISYMIGRRKSR TGYQSV in isoform LAMP-2B. 2 PublicationsAdd BLAST45
Alternative sequenceiVSP_003046372 – 415DCSAD…GYEQF → ECSLDDDTILIPIIVGAGLS GLIIVIVIAYLIGRRKTYAG YQTL in isoform LAMP-2C. CuratedAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05287 mRNA. Translation: AAA39412.1.
AK159272 mRNA. Translation: BAE34951.1.
AK032974 mRNA. Translation: BAC28106.1.
AK163933 mRNA. Translation: BAE37543.1.
AL513356 Genomic DNA. Translation: CAM17143.1.
AL513356 Genomic DNA. Translation: CAM17144.1.
CH466570 Genomic DNA. Translation: EDL29016.1.
CH466570 Genomic DNA. Translation: EDL29018.1.
BC138718 mRNA. Translation: AAI38719.1.
BC138723 mRNA. Translation: AAI38724.1.
M32017 mRNA. Translation: AAA39429.1.
M32018 Genomic DNA. Translation: AAA39430.1.
CCDSiCCDS30092.1. [P17047-1]
CCDS30093.1. [P17047-3]
CCDS72369.1. [P17047-2]
PIRiA35560.
RefSeqiNP_001017959.1. NM_001017959.2. [P17047-1]
NP_001277414.1. NM_001290485.1. [P17047-2]
NP_034815.2. NM_010685.4. [P17047-3]
UniGeneiMm.414016.
Mm.486.

Genome annotation databases

EnsembliENSMUST00000016678; ENSMUSP00000016678; ENSMUSG00000016534. [P17047-1]
ENSMUST00000061755; ENSMUSP00000052283; ENSMUSG00000016534. [P17047-3]
ENSMUST00000074913; ENSMUSP00000074448; ENSMUSG00000016534. [P17047-2]
GeneIDi16784.
KEGGimmu:16784.
UCSCiuc009szw.2. mouse. [P17047-1]
uc009szx.2. mouse. [P17047-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05287 mRNA. Translation: AAA39412.1.
AK159272 mRNA. Translation: BAE34951.1.
AK032974 mRNA. Translation: BAC28106.1.
AK163933 mRNA. Translation: BAE37543.1.
AL513356 Genomic DNA. Translation: CAM17143.1.
AL513356 Genomic DNA. Translation: CAM17144.1.
CH466570 Genomic DNA. Translation: EDL29016.1.
CH466570 Genomic DNA. Translation: EDL29018.1.
BC138718 mRNA. Translation: AAI38719.1.
BC138723 mRNA. Translation: AAI38724.1.
M32017 mRNA. Translation: AAA39429.1.
M32018 Genomic DNA. Translation: AAA39430.1.
CCDSiCCDS30092.1. [P17047-1]
CCDS30093.1. [P17047-3]
CCDS72369.1. [P17047-2]
PIRiA35560.
RefSeqiNP_001017959.1. NM_001017959.2. [P17047-1]
NP_001277414.1. NM_001290485.1. [P17047-2]
NP_034815.2. NM_010685.4. [P17047-3]
UniGeneiMm.414016.
Mm.486.

3D structure databases

ProteinModelPortaliP17047.
SMRiP17047.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201106. 3 interactors.
IntActiP17047. 7 interactors.
MINTiMINT-1857994.
STRINGi10090.ENSMUSP00000016678.

PTM databases

iPTMnetiP17047.
PhosphoSitePlusiP17047.
SwissPalmiP17047.

Proteomic databases

MaxQBiP17047.
PaxDbiP17047.
PeptideAtlasiP17047.
PRIDEiP17047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016678; ENSMUSP00000016678; ENSMUSG00000016534. [P17047-1]
ENSMUST00000061755; ENSMUSP00000052283; ENSMUSG00000016534. [P17047-3]
ENSMUST00000074913; ENSMUSP00000074448; ENSMUSG00000016534. [P17047-2]
GeneIDi16784.
KEGGimmu:16784.
UCSCiuc009szw.2. mouse. [P17047-1]
uc009szx.2. mouse. [P17047-3]

Organism-specific databases

CTDi3920.
MGIiMGI:96748. Lamp2.

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiP17047.
KOiK06528.
OMAiAEECFAD.
TreeFamiTF316339.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiLamp2. mouse.
PROiPR:P17047.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016534.
CleanExiMM_LAMP2.
ExpressionAtlasiP17047. baseline and differential.
GenevisibleiP17047. MM.

Family and domain databases

InterProiView protein in InterPro
IPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiView protein in Pfam
PF01299. Lamp. 1 hit.
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiView protein in PROSITE
PS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLAMP2_MOUSE
AccessioniPrimary (citable) accession number: P17047
Secondary accession number(s): A2A430, Q3TXG5, Q8BSG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 31, 2011
Last modified: March 15, 2017
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.