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Protein

Lysosome-associated membrane glycoprotein 2

Gene

Lamp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038). Binds target proteins, such as GAPDH, and targets them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871). Plays a role in lysosomal protein degradation in response to starvation (PubMed:11082038). Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity).By similarity2 Publications

GO - Biological processi

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome-associated membrane glycoprotein 2
Short name:
LAMP-2
Short name:
Lysosome-associated membrane protein 2
Alternative name(s):
CD107 antigen-like family member B
LGP-1101 Publication
LGP-961 Publication
Lysosomal membrane glycoprotein type B
Short name:
LGP-B
CD_antigen: CD107b
Gene namesi
Name:Lamp2
Synonyms:Lamp-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2990. Lamp2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 376LumenalSequence analysisAdd BLAST350
Transmembranei377 – 400HelicalPROSITE-ProRule annotationAdd BLAST24
Topological domaini401 – 411CytoplasmicPROSITE-ProRule annotation1 PublicationAdd BLAST11

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 263 PublicationsAdd BLAST26
ChainiPRO_000001711227 – 411Lysosome-associated membrane glycoprotein 2Add BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi30N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi38 ↔ 75PROSITE-ProRule annotation
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi149 ↔ 185PROSITE-ProRule annotation
Glycosylationi175N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi223N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi230N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi233 ↔ 266PROSITE-ProRule annotation
Glycosylationi243N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi261N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi318N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi332 ↔ 369PROSITE-ProRule annotation
Glycosylationi357N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Extensively N-glycosylated (PubMed:1794981, PubMed:18644871). Contains a minor proportion of O-linked glycans (PubMed:1794981). Contains sialylated glycans (PubMed:1794981).2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP17046.
PRIDEiP17046.

PTM databases

UniCarbKBiP17046.

Expressioni

Tissue specificityi

Detected in liver, kidney, spleen and macrophages (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000000164.
GenevisibleiQ6P6W1. RN.

Interactioni

Subunit structurei

Monomer (PubMed:18644871). Forms large homooligomers (PubMed:11082038, PubMed:18644871). Interacts (via its cytoplasmic region) with HSPA8 (PubMed:18644871). Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (PubMed:18644871). Interacts with MLLT11 (By similarity).By similarity2 Publications

Protein-protein interaction databases

IntActiP17046. 6 interactors.
MINTiMINT-2982158.
STRINGi10116.ENSRNOP00000000177.

Structurei

3D structure databases

SMRiP17046.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 188First lumenal domainAdd BLAST162
Regioni189 – 229HingeAdd BLAST41
Regioni230 – 376Second lumenal domainAdd BLAST147
Regioni402 – 405Important for binding and subsequent lysosomal degradation of target proteinsBy similarity4

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4818. Eukaryota.
ENOG410XQ96. LUCA.
GeneTreeiENSGT00530000063068.
HOGENOMiHOG000230942.
HOVERGENiHBG052303.
InParanoidiP17046.
KOiK06528.
OMAiPIIHTTV.
OrthoDBiEOG091G09EV.
PhylomeDBiP17046.
TreeFamiTF316339.

Family and domain databases

InterProiView protein in InterPro
IPR018134. LAMP_CS.
IPR002000. Lysosome-assoc_membr_glycop.
PANTHERiPTHR11506. PTHR11506. 1 hit.
PfamiView protein in Pfam
PF01299. Lamp. 1 hit.
PRINTSiPR00336. LYSASSOCTDMP.
PROSITEiView protein in PROSITE
PS00310. LAMP_1. 1 hit.
PS00311. LAMP_2. 1 hit.
PS51407. LAMP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLSPVTGS KLVLLFLFLG AVRSDALKLN LTDSKGTCLY AEWEMNFTIT
60 70 80 90 100
YEALKVNETV TITVPDKVTY NGSSCGDDKN GAKIMIQYGS TLSWAVNFTK
110 120 130 140 150
EASQYFINNI TLSYNTNDTK TFPGAVPKGI LTVIIPVGSQ LPLGVIFKCS
160 170 180 190 200
SVLTFNLSPV VQHYWGIHLQ AFVQNGTVSK HEQVCKEDKT ATTVAPIIHT
210 220 230 240 250
TVPSPTTTLT PTSIPVPTPT VGNYTISNGN ATCLLATMGL QLNITEEKVP
260 270 280 290 300
FIFNINPAIT NFTGSCQPQT AQLRLNNSQI KYLDFIFAVK NEKRFYLKEV
310 320 330 340 350
NVNMYLANGS AFHVSNNNLS FWDAPLGSSY MCNKEQVVSV SRTFQINTFN
360 370 380 390 400
LKVQPFNVTK GEYSTAQDCS ADEDNFLVPI AVGAALGGVL ILVLLAYFIG
410
LKRHHTGYEQ F
Length:411
Mass (Da):45,156
Last modified:February 15, 2017 - v3
Checksum:i001CFBA548AD3869
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4 – 8LSPVT → PLSGY in BAA14236 (PubMed:2590192).5
Sequence conflicti259I → T in BAA14236 (PubMed:2590192).1
Sequence conflicti259I → T in AAA41526 (PubMed:2142158).1
Sequence conflicti259I → T in AAH61990 (PubMed:15489334).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90211 mRNA. Translation: BAA14236.1.
AABR07041263 Genomic DNA. No translation available.
BC061990 mRNA. Translation: AAH61990.1.
M32016 mRNA. Translation: AAA41526.1.
PIRiA33664.
RefSeqiNP_058764.2. NM_017068.2.
UniGeneiRn.1722.

Genome annotation databases

EnsembliENSRNOT00000000177; ENSRNOP00000000177; ENSRNOG00000000164.
GeneIDi24944.
KEGGirno:24944.
UCSCiRGD:2990. rat.

Entry informationi

Entry nameiLAMP2_RAT
AccessioniPrimary (citable) accession number: P17046
Secondary accession number(s): F1LLX8, Q6P6W1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 15, 2017
Last modified: September 27, 2017
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families