Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lysosome-associated membrane glycoprotein 2

Gene

Lamp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038). Binds target proteins, such as GAPDH, and targets them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871). Plays a role in lysosomal protein degradation in response to starvation (PubMed:11082038). Required for the fusion of autophagosomes with lysosomes during autophagy. Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes. Required for normal degradation of the contents of autophagosomes. Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy

Enzyme and pathway databases

ReactomeiR-RNO-114608 Platelet degranulation
R-RNO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosome-associated membrane glycoprotein 2
Short name:
LAMP-2
Short name:
Lysosome-associated membrane protein 2
Alternative name(s):
CD107 antigen-like family member B
LGP-1101 Publication
LGP-961 Publication
Lysosomal membrane glycoprotein type B
Short name:
LGP-B
CD_antigen: CD107b
Gene namesi
Name:Lamp2
Synonyms:Lamp-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2990 Lamp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 376LumenalSequence analysisAdd BLAST350
Transmembranei377 – 400HelicalPROSITE-ProRule annotationAdd BLAST24
Topological domaini401 – 411CytoplasmicPROSITE-ProRule annotation1 PublicationAdd BLAST11

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 263 PublicationsAdd BLAST26
ChainiPRO_000001711227 – 411Lysosome-associated membrane glycoprotein 2Add BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi30N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi38 ↔ 75PROSITE-ProRule annotation
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi109N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi117N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi149 ↔ 185PROSITE-ProRule annotation
Glycosylationi175N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi223N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi230N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi233 ↔ 266PROSITE-ProRule annotation
Glycosylationi243N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi261N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi276N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi308N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi318N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi332 ↔ 369PROSITE-ProRule annotation
Glycosylationi357N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Extensively N-glycosylated (PubMed:1794981, PubMed:18644871). Contains a minor proportion of O-linked glycans (PubMed:1794981). Contains sialylated glycans (PubMed:1794981).2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP17046
PRIDEiP17046

PTM databases

UniCarbKBiP17046

Expressioni

Tissue specificityi

Detected in liver, kidney, spleen and macrophages (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000000164
GenevisibleiQ6P6W1 RN

Interactioni

Subunit structurei

Monomer (PubMed:18644871). Forms large homooligomers (PubMed:11082038, PubMed:18644871). Interacts (via its cytoplasmic region) with HSPA8 (PubMed:18644871). Interacts with HSP90 in the lysosome lumen; this enhances LAMP2 stability (PubMed:18644871). Interacts with MLLT11 (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiP17046, 6 interactors
MINTiP17046
STRINGi10116.ENSRNOP00000000177

Structurei

3D structure databases

SMRiP17046
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 188First lumenal domainAdd BLAST162
Regioni189 – 229HingeAdd BLAST41
Regioni230 – 376Second lumenal domainAdd BLAST147
Regioni402 – 405Important for binding and subsequent lysosomal degradation of target proteinsBy similarity4

Sequence similaritiesi

Belongs to the LAMP family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4818 Eukaryota
ENOG410XQ96 LUCA
GeneTreeiENSGT00530000063068
HOGENOMiHOG000230942
HOVERGENiHBG052303
InParanoidiP17046
KOiK06528
OMAiPIIHTTV
OrthoDBiEOG091G09EV
PhylomeDBiP17046
TreeFamiTF316339

Family and domain databases

InterProiView protein in InterPro
IPR018134 LAMP_CS
IPR002000 Lysosome-assoc_membr_glycop
PANTHERiPTHR11506 PTHR11506, 1 hit
PfamiView protein in Pfam
PF01299 Lamp, 1 hit
PRINTSiPR00336 LYSASSOCTDMP
PROSITEiView protein in PROSITE
PS00310 LAMP_1, 1 hit
PS00311 LAMP_2, 1 hit
PS51407 LAMP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P17046-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLSPVTGS KLVLLFLFLG AVRSDALKLN LTDSKGTCLY AEWEMNFTIT
60 70 80 90 100
YEALKVNETV TITVPDKVTY NGSSCGDDKN GAKIMIQYGS TLSWAVNFTK
110 120 130 140 150
EASQYFINNI TLSYNTNDTK TFPGAVPKGI LTVIIPVGSQ LPLGVIFKCS
160 170 180 190 200
SVLTFNLSPV VQHYWGIHLQ AFVQNGTVSK HEQVCKEDKT ATTVAPIIHT
210 220 230 240 250
TVPSPTTTLT PTSIPVPTPT VGNYTISNGN ATCLLATMGL QLNITEEKVP
260 270 280 290 300
FIFNINPAIT NFTGSCQPQT AQLRLNNSQI KYLDFIFAVK NEKRFYLKEV
310 320 330 340 350
NVNMYLANGS AFHVSNNNLS FWDAPLGSSY MCNKEQVVSV SRTFQINTFN
360 370 380 390 400
LKVQPFNVTK GEYSTAQDCS ADEDNFLVPI AVGAALGGVL ILVLLAYFIG
410
LKRHHTGYEQ F
Length:411
Mass (Da):45,156
Last modified:February 15, 2017 - v3
Checksum:i001CFBA548AD3869
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4 – 8LSPVT → PLSGY in BAA14236 (PubMed:2590192).5
Sequence conflicti259I → T in BAA14236 (PubMed:2590192).1
Sequence conflicti259I → T in AAA41526 (PubMed:2142158).1
Sequence conflicti259I → T in AAH61990 (PubMed:15489334).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90211 mRNA Translation: BAA14236.1
AABR07041263 Genomic DNA No translation available.
BC061990 mRNA Translation: AAH61990.1
M32016 mRNA Translation: AAA41526.1
PIRiA33664
RefSeqiNP_058764.2, NM_017068.2
UniGeneiRn.1722

Genome annotation databases

EnsembliENSRNOT00000000177; ENSRNOP00000000177; ENSRNOG00000000164
GeneIDi24944
KEGGirno:24944
UCSCiRGD:2990 rat

Entry informationi

Entry nameiLAMP2_RAT
AccessioniPrimary (citable) accession number: P17046
Secondary accession number(s): F1LLX8, Q6P6W1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: February 15, 2017
Last modified: May 23, 2018
This is version 126 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health