Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P17044 (MTBF_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BsuFI

Short name=M.BsuFI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase BsuFI
Gene names
Name:hsdFM
Synonyms:hsmF
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BsuFI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409Modification methylase BsuFI
PRO_0000087863

Regions

Domain101 – 402302SAM-dependent MTase C5-type

Sites

Active site1701 By similarity

Sequences

Sequence LengthMass (Da)Tools
P17044 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 9CBE3732596A3C20

FASTA40946,911
        10         20         30         40         50         60 
MRGGNRLGAG RKVIPESEKK KRKSVYITDK LYTRIMDTDI ENCNNFSQKC MALIELAMEN 

        70         80         90        100        110        120 
LNKNNQEHSV KRNNILMVRD TKSTYNKTNN NFEKQNRGIK LTFIDLFAGI GGIRLGFEDK 

       130        140        150        160        170        180 
YTKCVFSSEW DKYAAQTYEA NYGEKPHGDI TKINENDIPD QDVLLAGFPC QPFSNIGKRE 

       190        200        210        220        230        240 
GFAHERRNII FDVLRILKKK QPKMFLLENV KGLLTNDNGN TFRVILDNLK SLGYSVFYEV 

       250        260        270        280        290        300 
MDAQNFGLPQ RRERIVIVGF HPDLGINDFS FPKGNPDNKV PINAILEHNP TGYSISKRLQ 

       310        320        330        340        350        360 
ESYLFKKDDG KPQIVDFRCT YQVNTLVASY HKIQRLTGTF VKDGETGLRL FSELELKRLM 

       370        380        390        400 
GFPVDFKVPV SRTQMYRQFG NSVAVPMIKA VAGAMKERLL LAEMQVLKK 

« Hide

References

[1]"The amino acid sequence of the CCGG recognizing DNA methyltransferase M.BsuFI: implications for the analysis of sequence recognition by cytosine DNA methyltransferases."
Walter J., Noyer-Weidner M., Trautner T.A.
EMBO J. 9:1007-1013(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ISF18.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X51515 Genomic DNA. Translation: CAA35888.1.
PIRCTBSFI. S09247.

3D structure databases

ProteinModelPortalP17044.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3338. M.BsuFI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTBF_BACIU
AccessionPrimary (citable) accession number: P17044
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries