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Protein

Modification methylase BsuFI

Gene

hsdFM

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BsuFI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei170PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3338 M.BsuFI

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BsuFI (EC:2.1.1.37)
Short name:
M.BsuFI
Alternative name(s):
Cytosine-specific methyltransferase BsuFI
Gene namesi
Name:hsdFM
Synonyms:hsmF
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878631 – 409Modification methylase BsuFIAdd BLAST409

Proteomic databases

PRIDEiP17044

Structurei

3D structure databases

ProteinModelPortaliP17044
SMRiP17044
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 402SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST302

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P17044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGGNRLGAG RKVIPESEKK KRKSVYITDK LYTRIMDTDI ENCNNFSQKC
60 70 80 90 100
MALIELAMEN LNKNNQEHSV KRNNILMVRD TKSTYNKTNN NFEKQNRGIK
110 120 130 140 150
LTFIDLFAGI GGIRLGFEDK YTKCVFSSEW DKYAAQTYEA NYGEKPHGDI
160 170 180 190 200
TKINENDIPD QDVLLAGFPC QPFSNIGKRE GFAHERRNII FDVLRILKKK
210 220 230 240 250
QPKMFLLENV KGLLTNDNGN TFRVILDNLK SLGYSVFYEV MDAQNFGLPQ
260 270 280 290 300
RRERIVIVGF HPDLGINDFS FPKGNPDNKV PINAILEHNP TGYSISKRLQ
310 320 330 340 350
ESYLFKKDDG KPQIVDFRCT YQVNTLVASY HKIQRLTGTF VKDGETGLRL
360 370 380 390 400
FSELELKRLM GFPVDFKVPV SRTQMYRQFG NSVAVPMIKA VAGAMKERLL

LAEMQVLKK
Length:409
Mass (Da):46,911
Last modified:August 1, 1990 - v1
Checksum:i9CBE3732596A3C20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51515 Genomic DNA Translation: CAA35888.1
PIRiS09247 CTBSFI

Similar proteinsi

Entry informationi

Entry nameiMTBF_BACIU
AccessioniPrimary (citable) accession number: P17044
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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