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Protein

Modification methylase BsuFI

Gene

hsdFM

Organism
Bacillus subtilis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BsuFI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3338. M.BsuFI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BsuFI (EC:2.1.1.37)
Short name:
M.BsuFI
Alternative name(s):
Cytosine-specific methyltransferase BsuFI
Gene namesi
Name:hsdFM
Synonyms:hsmF
OrganismiBacillus subtilis
Taxonomic identifieri1423 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409Modification methylase BsuFIPRO_0000087863Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP17044.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 402302SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P17044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGGNRLGAG RKVIPESEKK KRKSVYITDK LYTRIMDTDI ENCNNFSQKC
60 70 80 90 100
MALIELAMEN LNKNNQEHSV KRNNILMVRD TKSTYNKTNN NFEKQNRGIK
110 120 130 140 150
LTFIDLFAGI GGIRLGFEDK YTKCVFSSEW DKYAAQTYEA NYGEKPHGDI
160 170 180 190 200
TKINENDIPD QDVLLAGFPC QPFSNIGKRE GFAHERRNII FDVLRILKKK
210 220 230 240 250
QPKMFLLENV KGLLTNDNGN TFRVILDNLK SLGYSVFYEV MDAQNFGLPQ
260 270 280 290 300
RRERIVIVGF HPDLGINDFS FPKGNPDNKV PINAILEHNP TGYSISKRLQ
310 320 330 340 350
ESYLFKKDDG KPQIVDFRCT YQVNTLVASY HKIQRLTGTF VKDGETGLRL
360 370 380 390 400
FSELELKRLM GFPVDFKVPV SRTQMYRQFG NSVAVPMIKA VAGAMKERLL

LAEMQVLKK
Length:409
Mass (Da):46,911
Last modified:August 1, 1990 - v1
Checksum:i9CBE3732596A3C20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51515 Genomic DNA. Translation: CAA35888.1.
PIRiS09247. CTBSFI.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51515 Genomic DNA. Translation: CAA35888.1.
PIRiS09247. CTBSFI.

3D structure databases

ProteinModelPortaliP17044.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3338. M.BsuFI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The amino acid sequence of the CCGG recognizing DNA methyltransferase M.BsuFI: implications for the analysis of sequence recognition by cytosine DNA methyltransferases."
    Walter J., Noyer-Weidner M., Trautner T.A.
    EMBO J. 9:1007-1013(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ISF18.

Entry informationi

Entry nameiMTBF_BACIU
AccessioniPrimary (citable) accession number: P17044
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: January 7, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.