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Protein

SPARC

Gene

Sparc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi272 – 28312By similarityAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • extracellular matrix binding Source: Ensembl

GO - Biological processi

  • bone development Source: Ensembl
  • cellular response to growth factor stimulus Source: Ensembl
  • heart development Source: RGD
  • inner ear development Source: RGD
  • lung development Source: RGD
  • negative regulation of angiogenesis Source: Ensembl
  • negative regulation of endothelial cell proliferation Source: Ensembl
  • ossification Source: RGD
  • positive regulation of endothelial cell migration Source: Ensembl
  • regulation of cell morphogenesis Source: Ensembl
  • response to cadmium ion Source: RGD
  • response to calcium ion Source: RGD
  • response to cAMP Source: RGD
  • response to cytokine Source: RGD
  • response to ethanol Source: RGD
  • response to glucocorticoid Source: RGD
  • response to gravity Source: RGD
  • response to L-ascorbic acid Source: RGD
  • response to lead ion Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to peptide hormone Source: RGD
  • signal transduction Source: InterPro
  • wound healing Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-3000178. ECM proteoglycans.
R-RNO-3000497. Scavenging by Class H Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC
Alternative name(s):
Basement-membrane protein 40
Short name:
BM-40
Osteonectin
Short name:
ON
Secreted protein acidic and rich in cysteine
Gene namesi
Name:Sparc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3742. Sparc.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: UniProtKB-SubCell
  • cell surface Source: Ensembl
  • cytoplasm Source: RGD
  • extracellular space Source: RGD
  • intracellular Source: RGD
  • membrane-bounded vesicle Source: RGD
  • nuclear matrix Source: Ensembl
  • nucleus Source: RGD
  • platelet alpha granule membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Chaini18 – 301284SPARCPRO_0000020306Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 81PROSITE-ProRule annotation
Disulfide bondi75 ↔ 91PROSITE-ProRule annotation
Disulfide bondi93 ↔ 128PROSITE-ProRule annotation
Disulfide bondi99 ↔ 121PROSITE-ProRule annotation
Disulfide bondi110 ↔ 147PROSITE-ProRule annotation
Glycosylationi114 – 1141N-linked (GlcNAc...)Curated
Disulfide bondi153 ↔ 263PROSITE-ProRule annotation
Disulfide bondi271 ↔ 287PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP16975.
PRIDEiP16975.

Expressioni

Gene expression databases

BgeeiENSRNOG00000012840.
GenevisibleiP16975. RN.

Interactioni

Protein-protein interaction databases

IntActiP16975. 1 interaction.
MINTiMINT-4996496.
STRINGi10116.ENSRNOP00000017486.

Structurei

3D structure databases

ProteinModelPortaliP16975.
SMRiP16975. Positions 69-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 9123Follistatin-likeAdd
BLAST
Domaini87 – 14963Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini259 – 29436EF-handAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi23 – 6846Asp/Glu-rich (acidic; binds calcium)Add
BLAST

Sequence similaritiesi

Belongs to the SPARC family.Curated
Contains 1 EF-hand domain.Curated
Contains 1 follistatin-like domain.Curated
Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4004. Eukaryota.
ENOG41101WW. LUCA.
GeneTreeiENSGT00510000046787.
HOGENOMiHOG000088654.
HOVERGENiHBG002746.
InParanoidiP16975.
OMAiKDFPRRM.
OrthoDBiEOG091G0CPG.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view]
PfamiPF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view]
SMARTiSM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ TEAAEEMVAE ETVVEETGLP VGANPVQVEM
60 70 80 90 100
GEFEEGAEET VEEVVAENPC QNHHCKHGKV CELDESNTPM CVCQDPTSCP
110 120 130 140 150
APIGEFEKVC SNDNKTFDSS CHFFATKCTL EGTKKGHKLH LDYIGPCKYI
160 170 180 190 200
APCLDSELTE FPLRMRDWLK NVLVTLYERD EGNNLLTEKQ KLRVKKIHEN
210 220 230 240 250
EKRLEAGDHP VELLARDFEK NYNMYIFPVH WQFGQLDQHP IDGYLSHTEL
260 270 280 290 300
APLRAPLIPM EHCTTRFFET CDLDNDKYIA LEEWAGCFGI KEQDINKDLV

I
Length:301
Mass (Da):34,296
Last modified:May 1, 2007 - v4
Checksum:iF522EA716C7EF1A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611V → P in BAA06029 (PubMed:8660135).Curated
Sequence conflicti67 – 671E → CN in BAA06029 (PubMed:8660135).Curated
Sequence conflicti82 – 843ELD → DG in BAA06029 (PubMed:8660135).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28875 mRNA. Translation: BAA06029.1.
Y13714 mRNA. Translation: CAA74042.1.
BC061777 mRNA. Translation: AAH61777.1.
PIRiA34572.
RefSeqiNP_036788.1. NM_012656.1.
UniGeneiRn.234316.
Rn.98989.

Genome annotation databases

EnsembliENSRNOT00000017486; ENSRNOP00000017486; ENSRNOG00000012840.
GeneIDi24791.
KEGGirno:24791.
UCSCiRGD:3742. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28875 mRNA. Translation: BAA06029.1.
Y13714 mRNA. Translation: CAA74042.1.
BC061777 mRNA. Translation: AAH61777.1.
PIRiA34572.
RefSeqiNP_036788.1. NM_012656.1.
UniGeneiRn.234316.
Rn.98989.

3D structure databases

ProteinModelPortaliP16975.
SMRiP16975. Positions 69-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP16975. 1 interaction.
MINTiMINT-4996496.
STRINGi10116.ENSRNOP00000017486.

Proteomic databases

PaxDbiP16975.
PRIDEiP16975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017486; ENSRNOP00000017486; ENSRNOG00000012840.
GeneIDi24791.
KEGGirno:24791.
UCSCiRGD:3742. rat.

Organism-specific databases

CTDi6678.
RGDi3742. Sparc.

Phylogenomic databases

eggNOGiKOG4004. Eukaryota.
ENOG41101WW. LUCA.
GeneTreeiENSGT00510000046787.
HOGENOMiHOG000088654.
HOVERGENiHBG002746.
InParanoidiP16975.
OMAiKDFPRRM.
OrthoDBiEOG091G0CPG.

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-3000178. ECM proteoglycans.
R-RNO-3000497. Scavenging by Class H Receptors.

Miscellaneous databases

PROiP16975.

Gene expression databases

BgeeiENSRNOG00000012840.
GenevisibleiP16975. RN.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
[Graphical view]
PfamiPF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
[Graphical view]
SMARTiSM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPRC_RAT
AccessioniPrimary (citable) accession number: P16975
Secondary accession number(s): O08953, Q6GSZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 136 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.