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Protein

SPARC

Gene

Sparc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi272 – 283By similarityAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • collagen binding Source: GO_Central
  • extracellular matrix binding Source: GO_Central

GO - Biological processi

  • bone development Source: Ensembl
  • cellular response to growth factor stimulus Source: Ensembl
  • heart development Source: RGD
  • inner ear development Source: RGD
  • lung development Source: RGD
  • negative regulation of angiogenesis Source: Ensembl
  • negative regulation of endothelial cell proliferation Source: Ensembl
  • ossification Source: RGD
  • pigmentation Source: Ensembl
  • positive regulation of endothelial cell migration Source: Ensembl
  • regulation of cell morphogenesis Source: Ensembl
  • response to cadmium ion Source: RGD
  • response to calcium ion Source: RGD
  • response to cAMP Source: RGD
  • response to cytokine Source: RGD
  • response to ethanol Source: RGD
  • response to glucocorticoid Source: RGD
  • response to gravity Source: RGD
  • response to L-ascorbic acid Source: RGD
  • response to lead ion Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to peptide hormone Source: RGD
  • signal transduction Source: InterPro
  • wound healing Source: RGD

Keywordsi

LigandCalcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-3000178. ECM proteoglycans.
R-RNO-3000497. Scavenging by Class H Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
SPARC
Alternative name(s):
Basement-membrane protein 40
Short name:
BM-40
Osteonectin
Short name:
ON
Secreted protein acidic and rich in cysteine
Gene namesi
Name:Sparc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3742. Sparc.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000002030618 – 301SPARCAdd BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 81PROSITE-ProRule annotation
Disulfide bondi75 ↔ 91PROSITE-ProRule annotation
Disulfide bondi93 ↔ 128PROSITE-ProRule annotation
Disulfide bondi99 ↔ 121PROSITE-ProRule annotation
Disulfide bondi110 ↔ 147PROSITE-ProRule annotation
Glycosylationi114N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi153 ↔ 263PROSITE-ProRule annotation
Disulfide bondi271 ↔ 287PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP16975.
PRIDEiP16975.

Expressioni

Gene expression databases

BgeeiENSRNOG00000012840.
GenevisibleiP16975. RN.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiP16975. 1 interactor.
MINTiMINT-4996496.
STRINGi10116.ENSRNOP00000017486.

Structurei

3D structure databases

ProteinModelPortaliP16975.
SMRiP16975.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini69 – 91Follistatin-likeAdd BLAST23
Domaini87 – 149Kazal-likePROSITE-ProRule annotationAdd BLAST63
Domaini259 – 294EF-handAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 68Asp/Glu-rich (acidic; binds calcium)Add BLAST46

Sequence similaritiesi

Belongs to the SPARC family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4004. Eukaryota.
ENOG41101WW. LUCA.
GeneTreeiENSGT00510000046787.
HOGENOMiHOG000088654.
HOVERGENiHBG002746.
InParanoidiP16975.
OMAiKDFPRRM.
OrthoDBiEOG091G0CPG.

Family and domain databases

InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR003645. Fol_N.
IPR015369. Follistatin/Osteonectin_EGF.
IPR002350. Kazal_dom.
IPR036058. Kazal_dom_sf.
IPR001999. Osteonectin_CS.
IPR019577. SPARC/Testican_Ca-bd-dom.
PfamiView protein in Pfam
PF09289. FOLN. 1 hit.
PF00050. Kazal_1. 1 hit.
PF10591. SPARC_Ca_bdg. 1 hit.
SMARTiView protein in SMART
SM00274. FOLN. 1 hit.
SM00280. KAZAL. 1 hit.
SUPFAMiSSF100895. SSF100895. 1 hit.
SSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS00612. OSTEONECTIN_1. 1 hit.
PS00613. OSTEONECTIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAWIFFLLC LAGRALAAPQ TEAAEEMVAE ETVVEETGLP VGANPVQVEM
60 70 80 90 100
GEFEEGAEET VEEVVAENPC QNHHCKHGKV CELDESNTPM CVCQDPTSCP
110 120 130 140 150
APIGEFEKVC SNDNKTFDSS CHFFATKCTL EGTKKGHKLH LDYIGPCKYI
160 170 180 190 200
APCLDSELTE FPLRMRDWLK NVLVTLYERD EGNNLLTEKQ KLRVKKIHEN
210 220 230 240 250
EKRLEAGDHP VELLARDFEK NYNMYIFPVH WQFGQLDQHP IDGYLSHTEL
260 270 280 290 300
APLRAPLIPM EHCTTRFFET CDLDNDKYIA LEEWAGCFGI KEQDINKDLV

I
Length:301
Mass (Da):34,296
Last modified:May 1, 2007 - v4
Checksum:iF522EA716C7EF1A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61V → P in BAA06029 (PubMed:8660135).Curated1
Sequence conflicti67E → CN in BAA06029 (PubMed:8660135).Curated1
Sequence conflicti82 – 84ELD → DG in BAA06029 (PubMed:8660135).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28875 mRNA. Translation: BAA06029.1.
Y13714 mRNA. Translation: CAA74042.1.
BC061777 mRNA. Translation: AAH61777.1.
PIRiA34572.
RefSeqiNP_036788.1. NM_012656.1.
UniGeneiRn.234316.
Rn.98989.

Genome annotation databases

EnsembliENSRNOT00000017486; ENSRNOP00000017486; ENSRNOG00000012840.
GeneIDi24791.
KEGGirno:24791.
UCSCiRGD:3742. rat.

Similar proteinsi

Entry informationi

Entry nameiSPRC_RAT
AccessioniPrimary (citable) accession number: P16975
Secondary accession number(s): O08953, Q6GSZ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: May 1, 2007
Last modified: October 25, 2017
This is version 146 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families