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Protein

ATP-binding cassette sub-family D member 3

Gene

Abcd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transporter involved in the transport of branched-chain fatty acids and C27 bile acids into the peroxisome; the latter function is a crucial step in bile acid biosynthesis. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi473 – 4808ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of substances Source: RGD
  • ATP binding Source: RGD
  • protein self-association Source: RGD

GO - Biological processi

  • fatty acid beta-oxidation Source: Ensembl
  • fatty acid biosynthetic process Source: UniProtKB
  • peroxisomal long-chain fatty acid import Source: RGD
  • peroxisome organization Source: Ensembl
  • response to drug Source: RGD
  • response to organic cyclic compound Source: RGD
  • very long-chain fatty acid catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family D member 3
Alternative name(s):
70 kDa peroxisomal membrane protein
Short name:
PMP70
Gene namesi
Name:Abcd3
Synonyms:Pmp70, Pxmp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2007. Abcd3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei84 – 10421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei126 – 14621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei224 – 24421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei313 – 33321HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: RGD
  • mitochondrial inner membrane Source: Ensembl
  • mitochondrion Source: UniProtKB
  • peroxisomal matrix Source: Ensembl
  • peroxisomal membrane Source: HGNC
  • peroxisome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 659658ATP-binding cassette sub-family D member 3PRO_0000093311Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi12 – 121N-linked (GlcNAc...)Sequence Analysis
Modified residuei61 – 611N6-acetyllysineBy similarity
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi206 – 2061N-linked (GlcNAc...)Sequence Analysis
Modified residuei260 – 2601N6-acetyllysineBy similarity
Modified residuei399 – 3991N6-acetyllysineBy similarity
Modified residuei533 – 5331N6-acetyllysineBy similarity
Modified residuei659 – 6591PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP16970.
PRIDEiP16970.

PTM databases

PhosphoSiteiP16970.

Expressioni

Gene expression databases

GenevisibleiP16970. RN.

Interactioni

Subunit structurei

Homodimer or heterodimer (Potential). Interacts with PEX19 (By similarity).By similarityCurated

Protein-protein interaction databases

BioGridi247310. 2 interactions.
STRINGi10116.ENSRNOP00000016739.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini85 – 372288ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini434 – 659226ABC transporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 199198Interaction with PEX19By similarityAdd
BLAST
Regioni2 – 124123Targeting to peroxisomesBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4178.
GeneTreeiENSGT00390000003950.
HOGENOMiHOG000206081.
HOVERGENiHBG050438.
InParanoidiP16970.
KOiK05677.
PhylomeDBiP16970.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005283. FA_transporter.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06472. ABC_membrane_2. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00954. 3a01203. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAFSKYLTA RNSSLAGAAF LLFCLLHKRR RALGLHGKKS GKPPLQNNEK
60 70 80 90 100
EGKKERAVVD KVFLSRLSQI LKIMVPRTFC KETGYLILIA VMLVSRTYCD
110 120 130 140 150
VWMIQNGTLI ESGIIGRSSK DFKRYLFNFI AAMPLISLVN NFLKYGLNEL
160 170 180 190 200
KLCFRVRLTR YLYEEYLQAF TYYKMGNLDN RIANPDQLLT QDVEKFCNSV
210 220 230 240 250
VDLYSNLSKP FLDIVLYIFK LTSAIGAQGP ASMMAYLLVS GLFLTRLRRP
260 270 280 290 300
IGKMTIMEQK YEGEYRFVNS RLITNSEEIA FYNGNKREKQ TIHSVFRKLV
310 320 330 340 350
EHLHNFIFFR FSMGFIDSII AKYIATVVGY LVVSRPFLDL AHPRHLHSTH
360 370 380 390 400
SELLEDYYQS GRMLLRMSQA LGRIVLAGRE MTRLAGFTAR ITELMQVLKD
410 420 430 440 450
LNHGKYERTM VSQQDKGIEG AQASPLIPGA GEIINADNII KFDHVPLATP
460 470 480 490 500
NGDILIQDLS FEVRSGANVL ICGPNGCGKS SLFRVLGELW PLFGGHLTKP
510 520 530 540 550
ERGKLFYVPQ RPYMTLGTLR DQVIYPDGKE DQKKKGISDQ VLKGYLDNVQ
560 570 580 590 600
LGHILEREGG WDSVQDWMDV LSGGEKQRMA MARLFYHKPQ FAILDECTSA
610 620 630 640 650
VSVDVEDYIY SHCRKVGITL FTVSHRKSLW KHHEYYLHMD GRGNYEFKKI

TEDTVEFGS
Length:659
Mass (Da):75,316
Last modified:January 23, 2007 - v3
Checksum:iCA567F3DBE80B169
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90038 mRNA. Translation: BAA14086.1.
PIRiA35723.
RefSeqiNP_036936.1. NM_012804.1.
UniGeneiRn.7024.

Genome annotation databases

EnsembliENSRNOT00000016739; ENSRNOP00000016739; ENSRNOG00000011929.
GeneIDi25270.
KEGGirno:25270.
UCSCiRGD:2007. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90038 mRNA. Translation: BAA14086.1.
PIRiA35723.
RefSeqiNP_036936.1. NM_012804.1.
UniGeneiRn.7024.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247310. 2 interactions.
STRINGi10116.ENSRNOP00000016739.

PTM databases

PhosphoSiteiP16970.

Proteomic databases

PaxDbiP16970.
PRIDEiP16970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016739; ENSRNOP00000016739; ENSRNOG00000011929.
GeneIDi25270.
KEGGirno:25270.
UCSCiRGD:2007. rat.

Organism-specific databases

CTDi5825.
RGDi2007. Abcd3.

Phylogenomic databases

eggNOGiCOG4178.
GeneTreeiENSGT00390000003950.
HOGENOMiHOG000206081.
HOVERGENiHBG050438.
InParanoidiP16970.
KOiK05677.
PhylomeDBiP16970.

Miscellaneous databases

NextBioi605953.
PROiP16970.

Gene expression databases

GenevisibleiP16970. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005283. FA_transporter.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06472. ABC_membrane_2. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR00954. 3a01203. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The 70-kDa peroxisomal membrane protein is a member of the Mdr (P-glycoprotein)-related ATP-binding protein superfamily."
    Kamijo K., Taketani S., Yokota S., Osumi T., Hashimoto T.
    J. Biol. Chem. 265:4534-4540(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Wistar.
    Tissue: Liver.
  2. "Alkaline density gradient floatation of membranes: polypeptide composition of the mammalian peroxisomal membrane."
    Gouveia A.M.M., Reguenga C., Oliveira M.E.M., Eckerskorn C., Sa-Miranda C., Azevedo J.E.
    Anal. Biochem. 274:270-277(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-15 AND 283-296.
    Tissue: Liver.

Entry informationi

Entry nameiABCD3_RAT
AccessioniPrimary (citable) accession number: P16970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.