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Protein

Alpha-amylase inhibitor BMAI-1

Gene

IAM1

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Could be involved in insect defense mechanisms. Inhibits insect-type alpha-amylase.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Alpha-amylase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase inhibitor BMAI-1
Alternative name(s):
Alpha-amylase flour inhibitor
Allergen: Hor v 1
Gene namesi
Name:IAM1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Involved in baker's asthma.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3328. Hor v 15.0101.
418. Hor v 15.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei‹1 – 141 PublicationAdd BLAST›14
ChainiPRO_000001435515 – 146Alpha-amylase inhibitor BMAI-1Add BLAST132

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Five disulfide bonds are present (Probable), which are essential for the inhibitor activity.
Glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Endosperm.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP16968.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JXPQ. Eukaryota.
ENOG41103SW. LUCA.

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR016309. A-amylase_inhib/allergen.
IPR002411. Allergen/amylase_inhib_rice.
IPR006106. Allergen/soft/tryp_amyl_inhib.
IPR006105. Allergen/tryp_amyl_inhib_CS.
IPR016140. Bifunc_inhib/LTP/seed_store.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PIRSFiPIRSF001657. Allergen/amylase_inhib. 1 hit.
PRINTSiPR00808. AMLASEINHBTR.
PR00809. RAGALLERGEN.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.
PROSITEiPS00426. CEREAL_TRYP_AMYL_INH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
PTSVAVDQGS MVSNSPGEWC WPGMGYPVYP FPRCRALVKS QCAGGQVVES
60 70 80 90 100
IQKDCCRQIA AIGDEWCICG ALGSMRGSMY KELGVALADD KATVAEVFPG
110 120 130 140
CRTEVMDRAV ASLPAVCNQY IPNTNGTDGV CYWLSYYQPP RQMSSR
Length:146
Mass (Da):15,816
Last modified:November 1, 1997 - v2
Checksum:i73F505186D1A48D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63517 mRNA. Translation: CAA45085.1.
PIRiS26197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63517 mRNA. Translation: CAA45085.1.
PIRiS26197.

3D structure databases

ProteinModelPortaliP16968.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3328. Hor v 15.0101.
418. Hor v 15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410JXPQ. Eukaryota.
ENOG41103SW. LUCA.

Family and domain databases

Gene3Di1.10.120.10. 1 hit.
InterProiIPR016309. A-amylase_inhib/allergen.
IPR002411. Allergen/amylase_inhib_rice.
IPR006106. Allergen/soft/tryp_amyl_inhib.
IPR006105. Allergen/tryp_amyl_inhib_CS.
IPR016140. Bifunc_inhib/LTP/seed_store.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PIRSFiPIRSF001657. Allergen/amylase_inhib. 1 hit.
PRINTSiPR00808. AMLASEINHBTR.
PR00809. RAGALLERGEN.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.
PROSITEiPS00426. CEREAL_TRYP_AMYL_INH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIAA1_HORVU
AccessioniPrimary (citable) accession number: P16968
Secondary accession number(s): Q43483
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.