P16968 (IAA1_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase inhibitor BMAI-1

Alternative name(s):
Alpha-amylase flour inhibitor
Allergen=Hor v 1

Gene names

Name:

IAM1

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	146 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could be involved in insect defense mechanisms. Inhibits insect-type alpha-amylase.
Subunit structure	Monomer.
Subcellular location	Secreted.
Tissue specificity	Endosperm.
Post-translational modification	Five disulfide bonds are present Probable, which are essential for the inhibitor activity. Glycosylated.
Allergenic properties	Causes an allergic reaction in human. Involved in baker's asthma.
Sequence similarities	Belongs to the protease inhibitor I6 (cereal trypsin/alpha-amylase inhibitor) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Alpha-amylase inhibitor
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	type I hypersensitivity Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 14	›14	Ref.2
	Chain	15 – 146	132	Alpha-amylase inhibitor BMAI-1		PRO_0000014355
Amino acid modifications
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P16968 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 73F505186D1A48D8	FASTA	146	15,816
	10 20 30 40 50 60 PTSVAVDQGS MVSNSPGEWC WPGMGYPVYP FPRCRALVKS QCAGGQVVES IQKDCCRQIA 70 80 90 100 110 120 AIGDEWCICG ALGSMRGSMY KELGVALADD KATVAEVFPG CRTEVMDRAV ASLPAVCNQY 130 140 IPNTNGTDGV CYWLSYYQPP RQMSSR

References

[1]	"A major barley allergen associated with baker's asthma disease is a glycosylated monomeric inhibitor of insect alpha-amylase: cDNA cloning and chromosomal location of the gene." Mena M., Sanchez-Monge R., Gomez L., Salcedo G., Carbonero P. Plant Mol. Biol. 20:451-458(1992) [PubMed: 1421148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Abyssinian 2231. Tissue: Endosperm.
[2]	"A barley flour inhibitor of insect alpha-amylase is a major allergen associated with baker's asthma disease." Barber D., Sanchez-Monge R., Gomez L., Carpizo J., Armentia A., Lopez-Otin C., Juan F., Salcedo G. FEBS Lett. 248:119-122(1989) [PubMed: 2785932] [Abstract] Cited for: PROTEIN SEQUENCE OF 15-34. Strain: cv. Bomi.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63517 mRNA. Translation: CAA45085.1.
PIR	S26197.
3D structure databases
ProteinModelPortal	P16968.
ModBase	Search...
Protein family/group databases
Allergome	3328. Hor v 15.0101. 418. Hor v 15.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P16968.
Gene expression databases
Genevestigator	P16968.
Family and domain databases
InterPro	IPR002411. Allergen/amylase_inhib_rice. IPR006106. Allergen/soft/tryp_amyl_inhib. IPR006105. Allergen/tryp_amyl_inhib_CS. IPR016140. Bifunc_inhib/LTP/seed_store. IPR003612. LTP/seed_store/tryp_amyl_inhib. IPR013771. Trypsin/amylase_inhib. [Graphical view]
Gene3D	G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00808. AMLASEINHBTR. PR00809. RAGALLERGEN.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
PROSITE	PS00426. CEREAL_TRYP_AMYL_INH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IAA1_HORVU

Accession

Primary (citable) accession number: P16968
Secondary accession number(s): Q43483

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 1, 1997
Last modified:	September 21, 2011
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program