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P16954 (GLGB_SYNE7) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Synpcc7942_1085
OrganismSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]
Taxonomic identifier1140 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length774 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Biophysicochemical properties

Temperature dependence:

Optimum temperature is about 35 degrees Celsius. HAMAP-Rule MF_00685

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed HAMAP-Rule MF_00685
Chain2 – 7747731,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188756

Sites

Active site4401Nucleophile By similarity
Active site4931Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P16954 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: C4C8885EF103CCE4

FASTA77489,195
        10         20         30         40         50         60 
MTGTTPLPSS SLSVEQVNRI ASNQEQNPFD ILGPHPYEHE GQAGWVIRAY LPEAQEAAVI 

        70         80         90        100        110        120 
CPALRREFAM HPVHHPHFFE TWVPEETLEI YQLRITEGER ERIIYDPYAF RSPLLTDYDI 

       130        140        150        160        170        180 
HLFAEGNHHR IYEKLGAHPC ELENVAGVNF AVWAPSARNV SILGDFNSWD GRKHQMARRS 

       190        200        210        220        230        240 
NGIWELFIPE LTVGAAYKYE IKNYDGHIYE KSDPYGFQQE VRPKTASIVA DLDRYTWGDA 

       250        260        270        280        290        300 
DWLERRRHQE PLRQPISVYE VHLGSWMHAS SDAIATDAQG KPLPPVPVAD LKPGARFLTY 

       310        320        330        340        350        360 
RELADRLIPY VLDLGYSHIE LLPIAEHPFD GSWGYQVTGY YAATSRYGSP EDFMYFVDRC 

       370        380        390        400        410        420 
HQNGIGVILD WVPGHFPKDG HGLAFFDGTH LYEHADSRQG EHREWGTLVF NYGRHEVRNF 

       430        440        450        460        470        480 
LAANALFWFD KYHIDGIRVD AVASMLYLDY NRKEGEWIPN EYGGRENIEA ADFLRQVNHL 

       490        500        510        520        530        540 
IFSYFPGALS IAEESTSWPM VSWPTYVGGL GFNLKWNMGW MHDMLDYFSM DPWFRQFHQN 

       550        560        570        580        590        600 
NVTFSIWYAF SENFMLALSH DEVVHGKSNL IGKMPGDEWQ KFANLRCLLG YMFTHPGKKT 

       610        620        630        640        650        660 
LFMGMEFGQW AEWNVWGDLE WHLLQYEPHQ GLKQFVKDLN HLYRNAPALY SEDCNQAGFE 

       670        680        690        700        710        720 
WIDCSDNRHS IVSFIRRAHE SDRFLVVVCN FTPQPHAHYR IGVPVAGFYR EIFNSDARSY 

       730        740        750        760        770 
GGSNMGNLGG KWTDEWSCHN RPYSLDLCLP PLTTLVLELA SGPESLSEAA NSPL 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the Synechococcus sp. PCC7942 branching enzyme gene (glgB): expression in Bacillus subtilis."
Kiel J.A.K.W., Boels J.M., Beldman G., Venema G.
Gene 89:77-84(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7942.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31544 Genomic DNA. Translation: AAB39038.1.
CP000100 Genomic DNA. Translation: ABB57115.1.
PIRJQ0550.
RefSeqYP_171174.1. NC_006576.1.
YP_400102.1. NC_007604.1.

3D structure databases

ProteinModelPortalP16954.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING1140.Synpcc7942_1085.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB57115; ABB57115; Synpcc7942_1085.
GeneID3198206.
3775035.
KEGGsyc:syc0464_c.
syf:Synpcc7942_1085.
PATRIC23787447. VBISynElo51371_1254.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycSYNEL:SYNPCC7942_1085-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_SYNE7
AccessionPrimary (citable) accession number: P16954
Secondary accession number(s): Q31PA4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries