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P16901

- POL_OMVVS

UniProt

P16901 - POL_OMVVS

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Protein

Pol polyprotein

Gene

pol

Organism
Ovine maedi visna related virus (strain South Africa) (SA-OMVV) (Ovine lentivirus)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
dUTP + H2O = dUMP + diphosphate.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei44 – 441PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri808 – 84942Integrase-typePROSITE-ProRule annotationAdd
BLAST
DNA bindingi1027 – 107953Integrase-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. dUTP diphosphatase activity Source: UniProtKB-EC
  4. exoribonuclease H activity Source: UniProtKB-EC
  5. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  6. RNA-DNA hybrid ribonuclease activity Source: InterPro
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. DNA recombination Source: UniProtKB-KW
  3. dUTP metabolic process Source: InterPro
  4. establishment of integrated proviral latency Source: UniProtKB-KW
  5. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Nucleotide metabolism, Viral genome integration, Virus entry into host cell

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Protein family/group databases

MEROPSiA02.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 4 chains:
Alternative name(s):
Retropepsin
Alternative name(s):
Exoribonuclease H (EC:3.1.13.2)
Integrase
Short name:
IN
Gene namesi
Name:pol
OrganismiOvine maedi visna related virus (strain South Africa) (SA-OMVV) (Ovine lentivirus)
Taxonomic identifieri11664 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusOvine/caprine lentivirus group
Virus hostiOvis aries (Sheep) [TaxID: 9940]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 120120ProteaseBy similarityPRO_0000038853Add
BLAST
Chaini121 – 671551Reverse transcriptase/ribonuclease HBy similarityPRO_0000038854Add
BLAST
Chaini672 – 805134Deoxyuridine 5'-triphosphate nucleotidohydrolaseBy similarityPRO_0000038855Add
BLAST
Chaini806 – 1086281IntegraseBy similarityPRO_0000038856Add
BLAST

Post-translational modificationi

Cleavage sites that yield the mature proteins remain to be determined.

Structurei

3D structure databases

ProteinModelPortaliP16901.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 11072Peptidase A2PROSITE-ProRule annotationAdd
BLAST
Domaini167 – 356190Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST
Domaini551 – 673123RNase HPROSITE-ProRule annotationAdd
BLAST
Domaini850 – 1010161Integrase catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated
Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
Contains 1 integrase-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 integrase-type zinc finger.PROSITE-ProRule annotation
Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
Contains 1 RNase H domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri808 – 84942Integrase-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
2.70.40.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view]
SUPFAMiSSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF51283. SSF51283. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16901-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
SNITAGKQQE GATCGAVRAP YVVTEAPPKI DIKVGTNWKK VLVDTGADRT
60 70 80 90 100
IVRYHDNSGI PTGRIKLQGI GGIIEGEKWD KVVIQYKEKR IEGTIVVLPS
110 120 130 140 150
SPVEVLGRDN MAKLDIGIIM ANLEEKKIPI TQVKLKEGCK GPHIAQWPLT
160 170 180 190 200
QEKLEGLKEI VDKLEKEGKV GRAPPHWTCN TPIFCIKKKS GKWRMLIDFR
210 220 230 240 250
ELNKQTEDLA EAQLGLPHPG GLQKKKHVTI LDIGDAYFTI PLYEPYRPYT
260 270 280 290 300
CFTMLSPNNL GPCTRYYWKV LPQGWKLSPS VYQFTMQEIL RDWIAKHPMI
310 320 330 340 350
QFGIYMDDIY IGSDLDIMKH REIVEELASY IAQYGFMLPE EKRQEGYPAK
360 370 380 390 400
WLGFELHPEK WRFQKHTLPE IKEGTITLNK LQKLVGDLVW RQSLIGKSIP
410 420 430 440 450
NILKLMEGDR ALQSERRIEL RHVKEWEECR RKLAEMEGNY YDEEKDVYGQ
460 470 480 490 500
IDWGDKAIEY IVFQERGKPL WVNVVHNIKN LSQSQQIIKA AQKLTQEVII
510 520 530 540 550
RIGKIPWILL PGKEEDWILE LQIGNITWMP SFWSCYRGSI RWKKRNVITE
560 570 580 590 600
VVEGPTYYTD GGKKNGKGSL GFIASTGVKF RKHEEGTNQQ LELRAIEEAC
610 620 630 640 650
KQGPEKMNIV TDSRYAYEFM RRNWDEEVIK NPIQARIMKL VHDKEQIGVH
660 670 680 690 700
WVPGHKGIPQ NEEIDKYISE IFLAREGSGI LPKRAEDAGY DLICPQEVCI
710 720 730 740 750
PAGQVRKIPI NLRINLKEDQ WAMVGTKSSF ASKGVFVQGG IIDSGYQGII
760 770 780 790 800
QVVVYNSNDK EVIIPQGRKF AQLILMPLIH EDLEAWGETR RTERGNQGFG
810 820 830 840 850
STGAYWIENI PLAEEDHSKW HQDAGSLHLD FGIPRTAAED IVQQCEVCQE
860 870 880 890 900
NKMPSTIRGS NRRGIDHWQV DYTHYEDKII LVWVETNSGL IYAERVKGET
910 920 930 940 950
GQEFRIMTIR WYGLFAPKSL QSDNGPAFVA EPTQLLMKYL GITHTTGIPW
960 970 980 990 1000
NPQSQALVER THQTLKNTIE KFVSMFASFD SAIAAALITL NIKRKGGLGT
1010 1020 1030 1040 1050
SPMDIFIFNK EQQRIQQQST RNQSKFRFCY YRVRKRGHPG EWLGPTQVLW
1060 1070 1080
EGEGAIVIKD KNLEKYLVIA KKDVKFIPQP KEIQTE
Length:1,086
Mass (Da):124,515
Last modified:August 1, 1990 - v1
Checksum:i099F9614679FC8BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31646 Genomic RNA. Translation: AAA66812.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31646 Genomic RNA. Translation: AAA66812.1 .

3D structure databases

ProteinModelPortali P16901.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi A02.006.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.200. 1 hit.
2.30.30.10. 1 hit.
2.40.70.10. 1 hit.
2.70.40.10. 1 hit.
3.30.420.10. 2 hits.
InterProi IPR001969. Aspartic_peptidase_AS.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR001037. Integrase_C_retrovir.
IPR001584. Integrase_cat-core.
IPR017856. Integrase_Zn-bd_dom-like_N.
IPR003308. Integrase_Zn-bd_dom_N.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
IPR010659. RVT_connect.
IPR010661. RVT_thumb.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
PF00552. IN_DBD_C. 1 hit.
PF02022. Integrase_Zn. 1 hit.
PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
PF06815. RVT_connect. 1 hit.
PF06817. RVT_thumb. 1 hit.
[Graphical view ]
SUPFAMi SSF46919. SSF46919. 1 hit.
SSF50122. SSF50122. 1 hit.
SSF50630. SSF50630. 1 hit.
SSF51283. SSF51283. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS51027. INTEGRASE_DBD. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
PS50876. ZF_INTEGRASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence analysis of SA-OMVV, a visna-related ovine lentivirus: phylogenetic history of lentiviruses."
    Querat G., Audoly G., Sonigo P., Vigne R.
    Virology 175:434-447(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOL_OMVVS
AccessioniPrimary (citable) accession number: P16901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 29, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3