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P16897

- BLP4_PSEAI

UniProt

P16897 - BLP4_PSEAI

Protein

Beta-lactamase PSE-4

Gene

pse4

Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Hydrolyzes both carbenicillin and oxacillin.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Acyl-ester intermediate

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    SABIO-RKP16897.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase PSE-4 (EC:3.5.2.6)
    Alternative name(s):
    Beta-lactamase CARB-1
    Carbenicillinase 1
    Gene namesi
    Name:pse4
    Synonyms:carB1
    OrganismiPseudomonas aeruginosa
    Taxonomic identifieri287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 288271Beta-lactamase PSE-4PRO_0000017047Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi72 ↔ 118By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    288
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi23 – 3513
    Beta strandi39 – 468
    Turni47 – 504
    Beta strandi51 – 566
    Helixi64 – 674
    Helixi68 – 8013
    Beta strandi89 – 913
    Helixi94 – 963
    Helixi104 – 1063
    Beta strandi110 – 1134
    Helixi114 – 12411
    Helixi127 – 13711
    Helixi140 – 14910
    Helixi163 – 1653
    Helixi178 – 1858
    Beta strandi191 – 1944
    Helixi196 – 20712
    Turni213 – 2153
    Helixi216 – 2194
    Beta strandi225 – 2328
    Helixi234 – 2363
    Beta strandi238 – 2469
    Beta strandi249 – 26012
    Helixi265 – 28420

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G68X-ray1.95A18-288[»]
    1G6AX-ray1.75A18-288[»]
    4ID4X-ray1.05A125-185[»]
    ProteinModelPortaliP16897.
    SMRiP16897. Positions 20-285.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16897.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni229 – 2313Substrate binding

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16897-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG    50
    EYWDYNGNQR FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY 100
    SPVIEKQVGQ AITLDDACFA TMTTSDNTAA NIILSAVGGP KGVTDFLRQI 150
    GDKETRLDRI EPDLNEGKLG DLRDTTTPKA IASTLNKFLF GSALSEMNQK 200
    KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI TAVVWSEHQA 250
    PIIVSIYLAQ TQASMEERND AIVKIGHSIF DVYTSQSR 288
    Length:288
    Mass (Da):31,406
    Last modified:August 1, 1990 - v1
    Checksum:i0E618F058720F3A5
    GO

    Sequence cautioni

    The sequence AAA25979.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05162 Genomic DNA. Translation: AAA25979.1. Different initiation.
    PIRiA35001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05162 Genomic DNA. Translation: AAA25979.1 . Different initiation.
    PIRi A35001.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G68 X-ray 1.95 A 18-288 [» ]
    1G6A X-ray 1.75 A 18-288 [» ]
    4ID4 X-ray 1.05 A 125-185 [» ]
    ProteinModelPortali P16897.
    SMRi P16897. Positions 20-285.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1744489.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P16897.

    Miscellaneous databases

    EvolutionaryTracei P16897.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the PSE-4 carbenicillinase gene and correlations with the Staphylococcus aureus PC1 beta-lactamase crystal structure."
      Boissinot M., Levesque R.C.
      J. Biol. Chem. 265:1225-1230(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Dalgleish.
      Transposon: Tn1405.

    Entry informationi

    Entry nameiBLP4_PSEAI
    AccessioniPrimary (citable) accession number: P16897
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Transposable element

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3