P16897 (BLP4_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase PSE-4
EC=3.5.2.6

Alternative name(s):
Beta-lactamase CARB-1
Carbenicillinase 1

Gene names

Name:	pse4
Synonyms:	carB1

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes both carbenicillin and oxacillin.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.
Sequence caution	The sequence AAA25979.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Transposable element
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Potential

Chain

18 – 288

271

Beta-lactamase PSE-4

PRO_0000017047

Regions

Region

229 – 231

3

Substrate binding

Sites

Active site

65

1

Acyl-ester intermediate

Amino acid modifications

Disulfide bond

72 ↔ 118

By similarity

Secondary structure

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288

Helix Strand Turn

Details...

Helix

23 – 35

13

Beta strand

39 – 46

8

Turn

47 – 50

4

Beta strand

51 – 56

6

Helix

64 – 67

4

Helix

68 – 80

13

Beta strand

89 – 91

3

Helix

94 – 96

3

Helix

104 – 106

3

Beta strand

110 – 113

4

Helix

114 – 124

11

Helix

127 – 136

10

Helix

139 – 149

11

Helix

163 – 165

3

Helix

178 – 190

13

Beta strand

191 – 194

4

Helix

196 – 207

12

Turn

213 – 215

3

Helix

216 – 219

4

Beta strand

225 – 232

8

Helix

234 – 236

3

Beta strand

238 – 246

9

Beta strand

249 – 260

12

Helix

265 – 284

20

Sequences

	Sequence		Length	Mass (Da)
	P16897 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 0E618F058720F3A5	FASTA	288	31,406
	10 20 30 40 50 60 MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG EYWDYNGNQR 70 80 90 100 110 120 FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY SPVIEKQVGQ AITLDDACFA 130 140 150 160 170 180 TMTTSDNTAA NIILSAVGGP KGVTDFLRQI GDKETRLDRI EPDLNEGKLG DLRDTTTPKA 190 200 210 220 230 240 IASTLNKFLF GSALSEMNQK KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI 250 260 270 280 TAVVWSEHQA PIIVSIYLAQ TQASMEERND AIVKIGHSIF DVYTSQSR

References

[1]	"Nucleotide sequence of the PSE-4 carbenicillinase gene and correlations with the Staphylococcus aureus PC1 beta-lactamase crystal structure." Boissinot M., Levesque R.C. J. Biol. Chem. 265:1225-1230(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Dalgleish. Transposon: Tn1405.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J05162 Genomic DNA. Translation: AAA25979.1. Different initiation.

PIR

A35001.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G68	X-ray	1.95	A	18-288	[»]
1G6A	X-ray	1.75	A	18-288	[»]

ProteinModelPortal

P16897.

SMR

P16897. Positions 20-285.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

SABIO-RK

P16897.

Family and domain databases

Gene3D

3.40.710.10. 1 hit.

InterPro

IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]

PRINTS

PR00118. BLACTAMASEA.

SUPFAM

SSF56601. PBP_transp_fold. 1 hit.

PROSITE

PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChEMBL

CHEMBL1744489.

EvolutionaryTrace

P16897.

Entry information

Entry name

BLP4_PSEAI

Accession

Primary (citable) accession number: P16897

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program