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P16897

- BLP4_PSEAI

UniProt

P16897 - BLP4_PSEAI

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Protein

Beta-lactamase PSE-4

Gene

pse4

Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes both carbenicillin and oxacillin.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Acyl-ester intermediate

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKP16897.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase PSE-4 (EC:3.5.2.6)
Alternative name(s):
Beta-lactamase CARB-1
Carbenicillinase 1
Gene namesi
Name:pse4
Synonyms:carB1
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 288271Beta-lactamase PSE-4PRO_0000017047Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 118By similarity

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
288
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 3513
Beta strandi39 – 468
Turni47 – 504
Beta strandi51 – 566
Helixi64 – 674
Helixi68 – 8013
Beta strandi89 – 913
Helixi94 – 963
Helixi104 – 1063
Beta strandi110 – 1134
Helixi114 – 12411
Helixi127 – 13711
Helixi140 – 14910
Helixi163 – 1653
Helixi178 – 1858
Beta strandi191 – 1944
Helixi196 – 20712
Turni213 – 2153
Helixi216 – 2194
Beta strandi225 – 2328
Helixi234 – 2363
Beta strandi238 – 2469
Beta strandi249 – 26012
Helixi265 – 28420

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G68X-ray1.95A18-288[»]
1G6AX-ray1.75A18-288[»]
4ID4X-ray1.05A145-185[»]
ProteinModelPortaliP16897.
SMRiP16897. Positions 20-285.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16897.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni229 – 2313Substrate binding

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16897-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG
60 70 80 90 100
EYWDYNGNQR FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY
110 120 130 140 150
SPVIEKQVGQ AITLDDACFA TMTTSDNTAA NIILSAVGGP KGVTDFLRQI
160 170 180 190 200
GDKETRLDRI EPDLNEGKLG DLRDTTTPKA IASTLNKFLF GSALSEMNQK
210 220 230 240 250
KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI TAVVWSEHQA
260 270 280
PIIVSIYLAQ TQASMEERND AIVKIGHSIF DVYTSQSR
Length:288
Mass (Da):31,406
Last modified:August 1, 1990 - v1
Checksum:i0E618F058720F3A5
GO

Sequence cautioni

The sequence AAA25979.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05162 Genomic DNA. Translation: AAA25979.1. Different initiation.
PIRiA35001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05162 Genomic DNA. Translation: AAA25979.1 . Different initiation.
PIRi A35001.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G68 X-ray 1.95 A 18-288 [» ]
1G6A X-ray 1.75 A 18-288 [» ]
4ID4 X-ray 1.05 A 145-185 [» ]
ProteinModelPortali P16897.
SMRi P16897. Positions 20-285.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1744489.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P16897.

Miscellaneous databases

EvolutionaryTracei P16897.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the PSE-4 carbenicillinase gene and correlations with the Staphylococcus aureus PC1 beta-lactamase crystal structure."
    Boissinot M., Levesque R.C.
    J. Biol. Chem. 265:1225-1230(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Dalgleish.
    Transposon: Tn1405.

Entry informationi

Entry nameiBLP4_PSEAI
AccessioniPrimary (citable) accession number: P16897
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3