Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P16897 (BLP4_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase PSE-4

EC=3.5.2.6
Alternative name(s):
Beta-lactamase CARB-1
Carbenicillinase 1
Gene names
Name:pse4
Synonyms:carB1
OrganismPseudomonas aeruginosa
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes both carbenicillin and oxacillin.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Sequence caution

The sequence AAA25979.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical term3D-structure
Transposable element
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 288271Beta-lactamase PSE-4
PRO_0000017047

Regions

Region229 – 2313Substrate binding

Sites

Active site651Acyl-ester intermediate

Amino acid modifications

Disulfide bond72 ↔ 118 By similarity

Secondary structure

............................................ 288
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P16897 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 0E618F058720F3A5

FASTA28831,406
        10         20         30         40         50         60 
MKFLLAFSLL IPSVVFASSS KFQQVEQDVK AIEVSLSARI GVSVLDTQNG EYWDYNGNQR 

        70         80         90        100        110        120 
FPLTSTFKTI ACAKLLYDAE QGKVNPNSTV EIKKADLVTY SPVIEKQVGQ AITLDDACFA 

       130        140        150        160        170        180 
TMTTSDNTAA NIILSAVGGP KGVTDFLRQI GDKETRLDRI EPDLNEGKLG DLRDTTTPKA 

       190        200        210        220        230        240 
IASTLNKFLF GSALSEMNQK KLESWMVNNQ VTGNLLRSVL PAGWNIADRS GAGGFGARSI 

       250        260        270        280 
TAVVWSEHQA PIIVSIYLAQ TQASMEERND AIVKIGHSIF DVYTSQSR 

« Hide

References

[1]"Nucleotide sequence of the PSE-4 carbenicillinase gene and correlations with the Staphylococcus aureus PC1 beta-lactamase crystal structure."
Boissinot M., Levesque R.C.
J. Biol. Chem. 265:1225-1230(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Dalgleish.
Transposon: Tn1405.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05162 Genomic DNA. Translation: AAA25979.1. Different initiation.
PIRA35001.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G68X-ray1.95A18-288[»]
1G6AX-ray1.75A18-288[»]
4ID4X-ray1.05A145-185[»]
ProteinModelPortalP16897.
SMRP16897. Positions 20-285.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL1744489.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP16897.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP16897.

Entry information

Entry nameBLP4_PSEAI
AccessionPrimary (citable) accession number: P16897
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references