Reviewed, UniProtKB/Swiss-Prot P16879 (FES_MOUSE)

Names and origin

Protein names

Recommended name:
    Proto-oncogene tyrosine-protein kinase Fes/Fps
    EC=2.7.10.2
Alternative name(s):
    Proto-oncogene c-Fes

Gene names

Name:	Fes
Synonyms:	Fps

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	822 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Disruption phenotype	Mice are fertile and healthy, display slightly reduced numbers of myeloid cells and are more sensitive to lipopolysaccharide (LPS). Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily. Contains 1 FCH domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Disease	Proto-oncogene
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from Experiment. Source: Reactome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 822	822	Proto-oncogene tyrosine-protein kinase Fes/Fps		PRO_0000088089
Regions
	Domain	1 – 96	96	FCH
	Domain	460 – 549	90	SH2
	Domain	561 – 818	258	Protein kinase
	Nucleotide binding	567 – 575	9	ATP By similarity
Sites
	Active site	683	1	Proton acceptor By similarity
	Binding site	590	1	ATP By similarity
Amino acid modifications
	Modified residue	67	1	Phosphoserine By similarity
	Modified residue	261	1	Phosphotyrosine By similarity
	Modified residue	408	1	Phosphoserine By similarity
	Modified residue	409	1	Phosphothreonine By similarity
	Modified residue	410	1	Phosphoserine By similarity
	Modified residue	411	1	Phosphoserine By similarity
	Modified residue	421	1	Phosphothreonine By similarity
	Modified residue	423	1	Phosphothreonine By similarity
	Modified residue	614	1	Phosphotyrosine By similarity
	Modified residue	713	1	Phosphotyrosine; by autocatalysis Ref.6
	Modified residue	716	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	110 – 111	2	QH → HS in CAA31138. Ref.1
	Sequence conflict	413 – 414	2	Missing in CAA31138. Ref.1
	Sequence conflict	413 – 414	2	Missing in AAN33122. Ref.2
	Sequence conflict	467	1	R → W in CAA31138. Ref.1
	Sequence conflict	477	1	S → T in CAA31138. Ref.1
	Sequence conflict	477	1	S → T in AAN33122. Ref.2
	Sequence conflict	500	1	Q → H in CAA31138. Ref.1
	Sequence conflict	500	1	Q → H in AAN33122. Ref.2
	Sequence conflict	509	1	S → L in CAA31138. Ref.1
	Sequence conflict	509	1	S → L in AAN33122. Ref.2
	Sequence conflict	664	1	V → M in CAA31138. Ref.1
	Sequence conflict	716 – 717	2	SA → CS in CAA31138. Ref.1
	Sequence conflict	716 – 717	2	SA → CS in AAA40012. Ref.5
	Sequence conflict	749	1	L → P in AAA40012. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P16879-1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 939280C56ACF25EE	FASTA	822	93,779
	10 20 30 40 50 60 MGFSSELCSP QGHGAVQQMQ EAELRLLEGM RKWMAQRVKS DREYAGLLHH MSLQDSGGQS 70 80 90 100 110 120 WSSGPDSPVS QSWAEITSQT ENLSRVLRQH AEDLNSGPLS KLSVLIRERQ HLRKTYNEQW 130 140 150 160 170 180 QQLQQELTKT HSQDIEKLKT QYRTLVRDST QARRKYQEAS KDKDRDKAKD KYVRSLWKLF 190 200 210 220 230 240 AHHNRYVLGV RAAQLHHHHH HRFMLPGLLQ SLQDLHEEMA GILKDILQEY LEISSLVQDD 250 260 270 280 290 300 VASIHRELAA AAARIQPEFE YLGFLRQYGS TPDVPPCVTF DESLLEDGEQ LEPGELQLNE 310 320 330 340 350 360 LTLESVQHTL TSVTDELAVA TKEVLSRQEM VSQLQRELQS EEQNTHPRER VQLLSKRQML 370 380 390 400 410 420 QEAIQGLQIA LCSQDKLQAQ QELLQSKMEQ LGTGEPPAVP LLQDDRHSTS STEQEREGGR 430 440 450 460 470 480 TPTLEILKSH FSGIFRPKFS IPPPLQLVPE VQKPLYEQLW YHGAIPRAEV AELLTHSGDF 490 500 510 520 530 540 LVRESQGKQE YVLSVMWDGQ PRHFIIQSSD NLYRLEGDGF PSIPLLITHL LSSQQPLTKK 550 560 570 580 590 600 SGVVLFRAVP KDKWVLKHED LVLGEQIGRG NFGEVFSGRL RADNTPVAVK SCRETLPPDL 610 620 630 640 650 660 KAKFLQEARI LKQYNHPNIV RLIGVCTQKQ PIYIVMELVQ GGDFLTFLRT EGARLRVKTL 670 680 690 700 710 720 LQMVGDAAAG MEYLESKCCI HRDLAARNCL VTEKNVLKIS DFGMSREEAD GIYAASAGLR 730 740 750 760 770 780 QVPVKWTAPE ALNYGRYSSE SDVWSFGILL WETFSLGASP YPNLTNQQTR EFVEKGHRLP 790 800 810 820 CPELCPDAVF RLMEQCWAYE PGQRPSFSII CQELHSIRKR HR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and structural analysis of murine c-fes cDNA clones." Wilks A.F., Kurban R.R. Oncogene 3:289-294(1988) [PubMed: 3060793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Enhanced endotoxin sensitivity in fps/fes-null mice with minimal defects in hematopoietic homeostasis." Zirngibl R.A., Senis Y., Greer P.A. Mol. Cell. Biol. 22:2472-2486(2002) [PubMed: 11909942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE. Strain: 129/SvJ.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Spleen.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family." Wilks A.F., Kurban R.R., Hovens C.M., Ralph S.J. Gene 85:67-74(1989) [PubMed: 2482828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 680-749.
[6]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-713, MASS SPECTROMETRY. Tissue: Mast cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X12616 mRNA. Translation: CAA31138.1. AF542394 Genomic DNA. Translation: AAN33122.1. M33421 mRNA. Translation: AAA40012.1. AK143639 mRNA. Translation: BAE25475.1. AK170418 mRNA. Translation: BAE41784.1. BC129919 mRNA. Translation: AAI29920.1.
IPI	IPI00109075.
PIR	I48347.
RefSeq	NP_034324.2.
UniGene	Mm.48757
3D structure databases
SMR	P16879. Positions 15-282, 449-821.
ModBase	Search...
Protein-protein interaction databases
STRING	P16879.
PTM databases
PhosphoSite	P16879.
Proteomic databases
PRIDE	P16879.
Genome annotation databases
Ensembl	ENSMUST00000080932; ENSMUSP00000079733; ENSMUSG00000053158; Mus musculus. [Genome view]
GeneID	14159.
KEGG	mmu:14159.
Organism-specific databases
CTD	14159.
MGI	MGI:95514. Fes.
Phylogenomic databases
eggNOG	roNOG11172.
HOGENOM	HBG446727.
HOVERGEN	P16879.
InParanoid	P16879.
OMA	RDSAQAK.
OrthoDB	EOG96HJW8.
PhylomeDB	P16879.
Enzyme and pathway databases
BRENDA	2.7.10.2. 244.
Gene expression databases
ArrayExpress	P16879.
Bgee	P16879.
CleanEx	MM_FES.
Genevestigator	P16879.
GermOnline	ENSMUSG00000053158. Mus musculus.
Family and domain databases
InterPro	IPR001060. FCH. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR020635. Tyr_Pkinase_cat_dom. IPR008266. Tyr_prot_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF00611. FCH. 1 hit. PF00017. SH2. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN.
SMART	SM00055. FCH. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS50133. FCH. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	285286.
SOURCE	Search...

Entry information

Entry name

FES_MOUSE

Accession

Primary (citable) accession number: P16879
Secondary accession number(s): Q3TD20, Q62122, Q8CG02

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 15, 2008
Last modified:	February 9, 2010
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)