P16863 (CXA1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gap junction alpha-1 protein

Alternative name(s):
Connexin-43
Short name=Cx43

Gene names

Name:

gja1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Expressed in most tissues. Highest levels found in eye and brain.
Developmental stage	Appears during organogenesis.
Sequence similarities	Belongs to the connexin family. Alpha-type (group II) subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	cell-cell signaling Inferred from electronic annotation. Source: InterPro heart development Inferred from electronic annotation. Source: InterPro transmembrane transport Inferred from electronic annotation. Source: GOC
Cellular_component	connexon complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 379	378	Gap junction alpha-1 protein		PRO_0000057806
Regions
	Topological domain	2 – 13	12	Cytoplasmic Potential
	Transmembrane	14 – 36	23	Helical; Potential
	Topological domain	37 – 76	40	Extracellular Potential
	Transmembrane	77 – 99	23	Helical; Potential
	Topological domain	100 – 154	55	Cytoplasmic Potential
	Transmembrane	155 – 177	23	Helical; Potential
	Topological domain	178 – 208	31	Extracellular Potential
	Transmembrane	209 – 231	23	Helical; Potential
	Topological domain	232 – 379	148	Cytoplasmic Potential
Amino acid modifications
	Disulfide bond	54 ↔ 192		By similarity
	Disulfide bond	187 ↔ 198		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P16863 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 4FD12B031F595DDD	FASTA	379	42,961
	10 20 30 40 50 60 MGDWSALGRL LDKVQAYSTA GGKVWLSVLF IFRILLLGTA VESAWGDEQS AFVCNTQQPG 70 80 90 100 110 120 CENVCYDKSF PISHVRFWVL QIIFVSTPTL LYLAHVFYLM RKEEKLNRKE EELKMVQNEG 130 140 150 160 170 180 GNVDMHLKQI EIKKFKYGLE EHGKVKMRGG LLRTYIISIL FKSVFEVGFI IIQWYMYGFS 190 200 210 220 230 240 LSAIYTCKRD PCPHQVDCFL SRPTEKTIFI WFMLIVSIVS LALNIIELFY VTYKSIKDGI 250 260 270 280 290 300 KGKKDPFSAT NDAVISGKEC GSPKYAYFNG CSSPTAPMSP PGYKLVTGER NPSSCRNYNK 310 320 330 340 350 360 QASEQNWANY SAEQNRMGQA GSTISNTHAQ PFDFSDEHQN TKKMAPGHEM QPLTILDQRP 370 SSRASSHASS RPRPDDLEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential regulation of the levels of three gap junction mRNAs in Xenopus embryos." Gimlich R.L., Kumar N.M., Gilula N.B. J. Cell Biol. 110:597-605(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17243 mRNA. Translation: CAA35108.1. BC073407 mRNA. Translation: AAH73407.1.
PIR	A34575.
RefSeq	NP_001079129.1. NM_001085660.1.
UniGene	Xl.1109.
3D structure databases
ProteinModelPortal	P16863.
SMR	P16863. Positions 258-379.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	373664.
KEGG	xla:373664.
Organism-specific databases
CTD	2697.
Xenbase	XB-GENE-876609. gja1.
Phylogenomic databases
HOVERGEN	HBG009576.
KO	K07372.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002261. Connexin43. IPR013124. Connexin43_C. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. [Graphical view]
PANTHER	PTHR11984. PTHR11984. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF03508. Connexin43. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01132. CONNEXINA1.
SMART	SM00037. CNX. 1 hit. SM01089. Connexin_CCC. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CXA1_XENLA

Accession

Primary (citable) accession number: P16863
Secondary accession number(s): Q6GNU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program