Reviewed,
UniProtKB/Swiss-Prot P16861 (K6PF1_YEAST)
Last modified
November 3, 2009.
Version 93.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 6-phosphofructokinase subunit alpha EC=2.7.1.11 Alternative name(s): Phosphofructokinase 1 Phosphohexokinase 6PF-1-K subunit alpha | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 987 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. |
| Enzyme regulation | Allosterically inhibited by ATP and activated by AMP and fructose 2,6-bisphosphate By similarity. |
| Pathway | |
| Subunit structure | Heterooctamer of 4 alpha and 4 beta chains. |
| Subcellular location | |
| Miscellaneous | Present with 89800 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Belongs to the phosphofructokinase family. Two domains subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Domain | Repeat |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | Allosteric enzyme Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from direct assay. Source: SGD proton transportInferred from genetic interaction. Source: UniProtKB |
| Cellular component | 6-phosphofructokinase complex Inferred from mutant phenotype. Source: SGD mitochondrionInferred from direct assay. Source: SGD |
| Molecular function | 6-phosphofructokinase activity Inferred from mutant phenotype. Source: SGD ATP bindingInferred from electronic annotation. Source: UniProtKB-KW magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| FMP31 | Q08742 | 1 | EBI-9428,EBI-33610 | |
| PFK2 | P16862 | 1 | EBI-9428,EBI-9435 | |
| RSA1 | Q08932 | 1 | EBI-9428,EBI-16186 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 987 | 987 | 6-phosphofructokinase subunit alpha | PRO_0000112045 | |||||
Regions | |||||||||
| Nucleotide binding | 225 – 229 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 383 – 387 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 400 – 416 | 17 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 356 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 414 | 1 | Magnesium; via carbonyl oxygen By similarity | ||||||
| Binding site | 391 | 1 | Substrate By similarity | ||||||
| Binding site | 488 | 1 | Substrate By similarity | ||||||
| Binding site | 491 | 1 | Substrate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 3 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 57 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 166 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 178 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 179 | 1 | Phosphoserine Ref.10 Ref.7 Ref.8 | ||||||
| Modified residue | 185 | 1 | Phosphoserine Ref.10 Ref.8 | ||||||
| Modified residue | 188 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 189 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 192 | 1 | Phosphoserine Ref.10 Ref.8 | ||||||
| Modified residue | 895 | 1 | Phosphoserine Ref.10 Ref.9 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The phosphofructokinase genes of yeast evolved from two duplication events." Heinisch J.J., Ritzel R.G., von Borstel R.C., Aguilera A., Rodicio R., Zimmermann F.K. Gene 78:309-321(1989) [PubMed: 2528496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII." Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C. Yeast 13:275-280(1997) [PubMed: 9090057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K.Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [4] | "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." van der Aart Q.J.M., Kleine K., Steensma H.Y. Yeast 12:385-390(1996) [PubMed: 8701610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 794-987. Strain: ATCC 204508 / S288c. |
| [5] | "Limited proteolysis of yeast phosphofructokinase. Sequence locations of cleavage sites created by the actions of different proteinases." Kopperschlaeger G., Baer J., Stellwagen E. Eur. J. Biochem. 217:527-533(1993) [PubMed: 8223596] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6; 90-97; 197-205 AND 914-921. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-179, MASS SPECTROMETRY. |
| [8] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-179; SER-185 AND SER-192, MASS SPECTROMETRY. |
| [9] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-895, MASS SPECTROMETRY. |
| [10] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3; SER-57; SER-166; THR-178; SER-179; SER-185; SER-188; SER-189; SER-192 AND SER-895, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M26943 Genomic DNA. Translation: AAA34859.1. Z73025 Genomic DNA. Translation: CAA97268.1. X87941 Genomic DNA. Translation: CAA61193.1. | |
| PIR | JQ0016. |
| RefSeq | NP_011756.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 3PFK based on UniProtKB P00512. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:1505N. |
| IntAct | P16861. 56 interactions. |
| STRING | P16861. |
Proteomic databases | |
| PeptideAtlas | P16861. |
| PRIDE | P16861. |
Genome annotation databases | |
| Ensembl | YGR240C; YGR240C; YGR240C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 853155. |
| GenomeReviews | Gene locus YGR240C in contig Y13135_GR. |
| KEGG | sce:YGR240C. |
| NMPDR | fig|4932.3.peg.2884. |
Organism-specific databases | |
| CYGD | YGR240c. |
| SGD | S000003472. PFK1. |
Phylogenomic databases | |
| HOGENOM | P16861. |
| OMA | DSTIGAY. |
Enzyme and pathway databases | |
| BRENDA | 2.7.1.11. 250. |
Gene expression databases | |
| ArrayExpress | P16861. |
| Genevestigator | P16861. |
| GermOnline | YGR240C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR009161. 6-phosphofructokinase_euk. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view] |
| Pfam | PF00365. PFK. 2 hits. [Graphical view] |
| PIRSF | PIRSF000533. ATP_PFK_euk. 1 hit. |
| PRINTS | PR00476. PHFRCTKINASE. |
| ProDom | PD000707. Ppfruckinase. 2 hits. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR02478. 6PF1K_euk. 1 hit. |
| PROSITE | PS00433. PHOSPHOFRUCTOKINASE. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 973249. |
Entry information
| Entry name | K6PF1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P16861 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VII Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names |

Clusters with


