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Protein

Natriuretic peptides B

Gene

NPPB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.1 Publication

GO - Molecular functioni

  • diuretic hormone activity Source: UniProtKB
  • hormone activity Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

  • body fluid secretion Source: UniProtKB
  • cell surface receptor signaling pathway Source: UniProtKB
  • cGMP biosynthetic process Source: UniProtKB
  • negative regulation of angiogenesis Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • positive regulation of renal sodium excretion Source: UniProtKB
  • positive regulation of urine volume Source: UniProtKB
  • receptor guanylyl cyclase signaling pathway Source: UniProtKB
  • regulation of blood pressure Source: UniProtKB
  • regulation of vascular permeability Source: UniProtKB
  • regulation of vasodilation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hormone, Vasoactive

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120937-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Natriuretic peptides B
Alternative name(s):
Gamma-brain natriuretic peptide
Cleaved into the following 16 chains:
Gene namesi
Name:NPPB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:7940. NPPB.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • extracellular space Source: UniProtKB
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Pharmaceutical usei

Available under the name Nesiritide (Scios). Used for the treatment of heart failure.

Organism-specific databases

DisGeNETi4879.
OpenTargetsiENSG00000120937.
PharmGKBiPA31734.

Chemistry databases

DrugBankiDB01136. Carvedilol.
DB06412. Oxymetholone.

Polymorphism and mutation databases

BioMutaiNPPB.
DMDMi113836.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000000153127 – 134Natriuretic peptides BAdd BLAST108
PeptideiPRO_0000001532103 – 134Brain natriuretic peptide 32Add BLAST32
PeptideiPRO_0000364993103 – 132BNP(1-30)Add BLAST30
PeptideiPRO_0000364994103 – 131BNP(1-29)Add BLAST29
PeptideiPRO_0000364995103 – 130BNP(1-28)Add BLAST28
PeptideiPRO_0000364996104 – 133BNP(2-31)Add BLAST30
PeptideiPRO_0000364997105 – 134BNP(3-32)Add BLAST30
PeptideiPRO_0000364998105 – 132BNP(3-30)Add BLAST28
PeptideiPRO_0000364999105 – 131BNP(3-29)Add BLAST27
PeptideiPRO_0000365000106 – 134BNP(4-32)Add BLAST29
PeptideiPRO_0000365001106 – 133BNP(4-31)Add BLAST28
PeptideiPRO_0000365002106 – 132BNP(4-30)Add BLAST27
PeptideiPRO_0000365003106 – 131BNP(4-29)Add BLAST26
PeptideiPRO_0000365004106 – 129BNP(4-27)Add BLAST24
PeptideiPRO_0000365005107 – 134BNP(5-32)Add BLAST28
PeptideiPRO_0000365006107 – 133BNP(5-31)Add BLAST27
PeptideiPRO_0000365007107 – 131BNP(5-29)Add BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi62O-linked (HexNAc...); Partial1 Publication1
Glycosylationi63O-linked (HexNAc...)1 Publication1
Glycosylationi70O-linked (HexNAc...)1 Publication1
Glycosylationi74O-linked (HexNAc...)1 Publication1
Glycosylationi79O-linked (HexNAc...)1 Publication1
Glycosylationi84O-linked (HexNAc...); Partial1 Publication1
Glycosylationi97O-linked (HexNAc...)1 Publication1
Disulfide bondi112 ↔ 1281 Publication

Post-translational modificationi

The brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei104 – 105Cleavage; by FAP1 Publication2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP16860.
PeptideAtlasiP16860.
PRIDEiP16860.

PTM databases

iPTMnetiP16860.
PhosphoSitePlusiP16860.

Miscellaneous databases

PMAP-CutDBP16860.

Expressioni

Tissue specificityi

Brain and also in atria, but at much lower levels than ANP.

Gene expression databases

BgeeiENSG00000120937.
CleanExiHS_NPPB.
GenevisibleiP16860. HS.

Organism-specific databases

HPAiHPA054960.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRT40Q6A1623EBI-747044,EBI-10171697
KRTAP10-9P604113EBI-747044,EBI-10172052
NOTCH2NLQ7Z3S93EBI-747044,EBI-945833
PSMA3P257883EBI-747044,EBI-348380

GO - Molecular functioni

  • diuretic hormone activity Source: UniProtKB
  • hormone activity Source: UniProtKB
  • receptor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110939. 10 interactors.
IntActiP16860. 10 interactors.
MINTiMINT-1434895.
STRINGi9606.ENSP00000365651.

Structurei

Secondary structure

1134
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi121 – 123Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YK1X-ray2.90E111-131[»]
3N56X-ray3.10C/D103-134[»]
DisProtiDP00551.
ProteinModelPortaliP16860.
SMRiP16860.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16860.

Family & Domainsi

Sequence similaritiesi

Belongs to the natriuretic peptide family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J2F8. Eukaryota.
ENOG410ZE1B. LUCA.
GeneTreeiENSGT00510000049822.
HOGENOMiHOG000033937.
HOVERGENiHBG004227.
InParanoidiP16860.
KOiK12335.
OMAiSGCFGRK.
OrthoDBiEOG091G0T3N.
PhylomeDBiP16860.
TreeFamiTF106304.

Family and domain databases

InterProiIPR000663. Natr_peptide.
IPR030480. Natr_peptide_CS.
IPR002408. Natriuretic_peptide_brain.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00712. BNATPEPTIDE.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16860-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPQTAPSRA LLLLLFLHLA FLGGRSHPLG SPGSASDLET SGLQEQRNHL
60 70 80 90 100
QGKLSELQVE QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA
110 120 130
PRSPKMVQGS GCFGRKMDRI SSSSGLGCKV LRRH
Length:134
Mass (Da):14,726
Last modified:August 1, 1990 - v1
Checksum:iDC884D9408462146
GO

Sequence cautioni

The sequence BAA90441 differs from that shown. Reason: Frameshift at position 44.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01458025R → L.Corresponds to variant rs5227dbSNPEnsembl.1
Natural variantiVAR_01458147R → H.Corresponds to variant rs5229dbSNPEnsembl.1
Natural variantiVAR_01458293M → L.Corresponds to variant rs5230dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31776 Genomic DNA. Translation: AAA35603.1.
M25296 mRNA. Translation: AAA36355.1.
AB037521 Genomic DNA. Translation: BAA90441.1. Frameshift.
CR541976 mRNA. Translation: CAG46774.1.
CR542003 mRNA. Translation: CAG46800.1.
EU326309 Genomic DNA. Translation: ACA05917.1.
AL021155 Genomic DNA. Translation: CAA15956.1.
CH471130 Genomic DNA. Translation: EAW71718.1.
BC025785 mRNA. Translation: AAH25785.1.
CCDSiCCDS140.1.
PIRiA36736. AWHUB.
RefSeqiNP_002512.1. NM_002521.2.
UniGeneiHs.219140.

Genome annotation databases

EnsembliENST00000376468; ENSP00000365651; ENSG00000120937.
GeneIDi4879.
KEGGihsa:4879.
UCSCiuc001atj.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Brain natriuretic peptide entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31776 Genomic DNA. Translation: AAA35603.1.
M25296 mRNA. Translation: AAA36355.1.
AB037521 Genomic DNA. Translation: BAA90441.1. Frameshift.
CR541976 mRNA. Translation: CAG46774.1.
CR542003 mRNA. Translation: CAG46800.1.
EU326309 Genomic DNA. Translation: ACA05917.1.
AL021155 Genomic DNA. Translation: CAA15956.1.
CH471130 Genomic DNA. Translation: EAW71718.1.
BC025785 mRNA. Translation: AAH25785.1.
CCDSiCCDS140.1.
PIRiA36736. AWHUB.
RefSeqiNP_002512.1. NM_002521.2.
UniGeneiHs.219140.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YK1X-ray2.90E111-131[»]
3N56X-ray3.10C/D103-134[»]
DisProtiDP00551.
ProteinModelPortaliP16860.
SMRiP16860.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110939. 10 interactors.
IntActiP16860. 10 interactors.
MINTiMINT-1434895.
STRINGi9606.ENSP00000365651.

Chemistry databases

DrugBankiDB01136. Carvedilol.
DB06412. Oxymetholone.

PTM databases

iPTMnetiP16860.
PhosphoSitePlusiP16860.

Polymorphism and mutation databases

BioMutaiNPPB.
DMDMi113836.

Proteomic databases

PaxDbiP16860.
PeptideAtlasiP16860.
PRIDEiP16860.

Protocols and materials databases

DNASUi4879.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376468; ENSP00000365651; ENSG00000120937.
GeneIDi4879.
KEGGihsa:4879.
UCSCiuc001atj.4. human.

Organism-specific databases

CTDi4879.
DisGeNETi4879.
GeneCardsiNPPB.
HGNCiHGNC:7940. NPPB.
HPAiHPA054960.
MIMi600295. gene.
neXtProtiNX_P16860.
OpenTargetsiENSG00000120937.
PharmGKBiPA31734.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J2F8. Eukaryota.
ENOG410ZE1B. LUCA.
GeneTreeiENSGT00510000049822.
HOGENOMiHOG000033937.
HOVERGENiHBG004227.
InParanoidiP16860.
KOiK12335.
OMAiSGCFGRK.
OrthoDBiEOG091G0T3N.
PhylomeDBiP16860.
TreeFamiTF106304.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120937-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP16860.
GeneWikiiBrain_natriuretic_peptide.
GenomeRNAii4879.
PMAP-CutDBP16860.
PROiP16860.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120937.
CleanExiHS_NPPB.
GenevisibleiP16860. HS.

Family and domain databases

InterProiIPR000663. Natr_peptide.
IPR030480. Natr_peptide_CS.
IPR002408. Natriuretic_peptide_brain.
[Graphical view]
PfamiPF00212. ANP. 1 hit.
[Graphical view]
PRINTSiPR00712. BNATPEPTIDE.
SMARTiSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANFB_HUMAN
AccessioniPrimary (citable) accession number: P16860
Secondary accession number(s): B0ZBE9, Q6FGY0, Q9P2Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 30, 2016
This is version 165 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.