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P16860 (ANFB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Natriuretic peptides B
Alternative name(s):
Gamma-brain natriuretic peptide
Gene names
Name:NPPB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length134 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Ref.11

Subcellular location

Secreted.

Tissue specificity

Brain and also in atria, but at much lower levels than ANP.

Pharmaceutical use

Available under the name Nesiritide (Scios). Used for the treatment of heart failure.

Sequence similarities

Belongs to the natriuretic peptide family.

Sequence caution

The sequence BAA90441.1 differs from that shown. Reason: Frameshift at position 44.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionHormone
Vasoactive
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Pharmaceutical
Reference proteome
Gene Ontology (GO)
   Biological_processbody fluid secretion

Traceable author statement PubMed 14960748. Source: UniProtKB

cGMP biosynthetic process

Inferred from direct assay Ref.11. Source: UniProtKB

cell surface receptor signaling pathway

Non-traceable author statement PubMed 12727915. Source: UniProtKB

negative regulation of angiogenesis

Traceable author statement PubMed 14737067. Source: UniProtKB

negative regulation of cell growth

Non-traceable author statement PubMed 12727915. Source: UniProtKB

positive regulation of renal sodium excretion

Traceable author statement PubMed 14960748. Source: UniProtKB

positive regulation of urine volume

Traceable author statement PubMed 14737067. Source: UniProtKB

receptor guanylyl cyclase signaling pathway

Inferred from direct assay Ref.11. Source: UniProtKB

regulation of blood pressure

Non-traceable author statement PubMed 12727915. Source: UniProtKB

regulation of blood vessel size

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of vascular permeability

Traceable author statement PubMed 14737067. Source: UniProtKB

regulation of vasodilation

Non-traceable author statement PubMed 7601467. Source: UniProtKB

   Cellular_componentextracellular region

Traceable author statement PubMed 14960748. Source: UniProtKB

extracellular space

Non-traceable author statement PubMed 7601467. Source: UniProtKB

protein complex

Inferred from direct assay PubMed 23382219. Source: MGI

   Molecular_functiondiuretic hormone activity

Traceable author statement PubMed 14960748. Source: UniProtKB

hormone activity

Non-traceable author statement PubMed 7601467. Source: UniProtKB

receptor binding

Inferred from direct assay Ref.11. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.9
Chain27 – 134108Natriuretic peptides B
PRO_0000001531
Peptide103 – 13432Brain natriuretic peptide 32 Ref.10
PRO_0000001532
Peptide103 – 13230BNP(1-30)
PRO_0000364993
Peptide103 – 13129BNP(1-29)
PRO_0000364994
Peptide103 – 13028BNP(1-28)
PRO_0000364995
Peptide104 – 13330BNP(2-31)
PRO_0000364996
Peptide105 – 13430BNP(3-32)
PRO_0000364997
Peptide105 – 13228BNP(3-30)
PRO_0000364998
Peptide105 – 13127BNP(3-29)
PRO_0000364999
Peptide106 – 13429BNP(4-32)
PRO_0000365000
Peptide106 – 13328BNP(4-31)
PRO_0000365001
Peptide106 – 13227BNP(4-30)
PRO_0000365002
Peptide106 – 13126BNP(4-29)
PRO_0000365003
Peptide106 – 12924BNP(4-27)
PRO_0000365004
Peptide107 – 13428BNP(5-32)
PRO_0000365005
Peptide107 – 13327BNP(5-31)
PRO_0000365006
Peptide107 – 13125BNP(5-29)
PRO_0000365007

Amino acid modifications

Glycosylation621O-linked (HexNAc...); Partial Ref.12
Glycosylation631O-linked (HexNAc...) Ref.12
Glycosylation701O-linked (HexNAc...) Ref.12
Glycosylation741O-linked (HexNAc...) Ref.12
Glycosylation791O-linked (HexNAc...) Ref.12
Glycosylation841O-linked (HexNAc...); Partial Ref.12
Glycosylation971O-linked (HexNAc...) Ref.12
Disulfide bond112 ↔ 128 Ref.14

Natural variations

Natural variant251R → L.
Corresponds to variant rs5227 [ dbSNP | Ensembl ].
VAR_014580
Natural variant471R → H.
Corresponds to variant rs5229 [ dbSNP | Ensembl ].
VAR_014581
Natural variant931M → L.
Corresponds to variant rs5230 [ dbSNP | Ensembl ].
VAR_014582

Secondary structure

... 134
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P16860 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: DC884D9408462146

FASTA13414,726
        10         20         30         40         50         60 
MDPQTAPSRA LLLLLFLHLA FLGGRSHPLG SPGSASDLET SGLQEQRNHL QGKLSELQVE 

        70         80         90        100        110        120 
QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA PRSPKMVQGS GCFGRKMDRI 

       130 
SSSSGLGCKV LRRH 

« Hide

References

« Hide 'large scale' references
[1]"Human and canine gene homologs of porcine brain natriuretic peptide."
Seilhamer J.J., Arfsten A., Miller J.A., Lundquist P., Scarborough R.M., Lewicki J.A., Porter J.G.
Biochem. Biophys. Res. Commun. 165:650-658(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning and sequence analysis of cDNA encoding a precursor for human brain natriuretic peptide."
Sudoh T., Maekawa K., Kojima M., Minamino N., Kangawa K., Matsuo H.
Biochem. Biophys. Res. Commun. 159:1427-1434(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Silver Project."
Kitano T., Kobayakawa H., Saitou N.
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]NHLBI resequencing and genotyping service (RS&G)
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Pancreas and Spleen.
[9]"Isolation and identification of human brain natriuretic peptides in cardiac atrium."
Hino J., Tateyaa H., Minamino N., Kangawa K., Matsuo H.
Biochem. Biophys. Res. Commun. 167:693-700(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-58 AND 103-134.
[10]"Isolation and sequence determination of human brain natriuretic peptide in human atrium."
Kambayashi Y., Nakao K., Mukoyama M., Saito Y., Ogawa Y., Shiono S., Inouye K., Yoshida N., Imura H.
FEBS Lett. 259:341-345(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 103-134.
[11]"Selective activation of the B natriuretic peptide receptor by C-type natriuretic peptide (CNP)."
Koller K.J., Lowe D.G., Bennett G.L., Minamino N., Kangawa K., Matsuo H., Goeddel D.V.
Science 252:120-123(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"The precursor to B-type natriuretic peptide is an O-linked glycoprotein."
Schellenberger U., O'Rear J., Guzzetta A., Jue R.A., Protter A.A., Pollitt N.S.
Arch. Biochem. Biophys. 451:160-166(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT THR-62; SER-63; SER-70; THR-74; SER-79; THR-84 AND THR-97.
[13]"Detection of endogenous B-type natriuretic peptide at very low concentrations in patients with heart failure."
Niederkofler E.E., Kiernan U.A., O'Rear J., Menon S., Saghir S., Protter A.A., Nelson R.W., Schellenberger U.
Circ. Heart Fail. 1:258-264(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING, IDENTIFICATION BY MASS SPECTROMETRY.
[14]"Structural determinants of natriuretic peptide receptor specificity and degeneracy."
He X.-L., Dukkipati A., Garcia K.C.
J. Mol. Biol. 361:698-714(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 111-131 IN COMPLEX WITH NPR3, DISULFIDE BOND.
+Additional computationally mapped references.

Web resources

Wikipedia

Brain natriuretic peptide entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31776 Genomic DNA. Translation: AAA35603.1.
M25296 mRNA. Translation: AAA36355.1.
AB037521 Genomic DNA. Translation: BAA90441.1. Frameshift.
CR541976 mRNA. Translation: CAG46774.1.
CR542003 mRNA. Translation: CAG46800.1.
EU326309 Genomic DNA. Translation: ACA05917.1.
AL021155 Genomic DNA. Translation: CAA15956.1.
CH471130 Genomic DNA. Translation: EAW71718.1.
BC025785 mRNA. Translation: AAH25785.1.
CCDSCCDS140.1.
PIRAWHUB. A36736.
RefSeqNP_002512.1. NM_002521.2.
UniGeneHs.219140.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YK1X-ray2.90E111-131[»]
3N56X-ray3.10C/D103-134[»]
DisProtDP00551.
ProteinModelPortalP16860.
SMRP16860. Positions 103-132.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110939. 6 interactions.
IntActP16860. 3 interactions.
MINTMINT-1434895.
STRING9606.ENSP00000365651.

Chemistry

DrugBankDB01136. Carvedilol.
DB04899. Nesiritide.
DB00624. Testosterone.

PTM databases

PhosphoSiteP16860.

Polymorphism databases

DMDM113836.

Proteomic databases

PaxDbP16860.
PRIDEP16860.

Protocols and materials databases

DNASU4879.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000376468; ENSP00000365651; ENSG00000120937.
GeneID4879.
KEGGhsa:4879.
UCSCuc001atj.3. human.

Organism-specific databases

CTD4879.
GeneCardsGC01M011918.
HGNCHGNC:7940. NPPB.
MIM600295. gene.
neXtProtNX_P16860.
PharmGKBPA31734.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG67899.
HOGENOMHOG000206568.
HOVERGENHBG004227.
KOK12335.
OMASGCFGRK.
OrthoDBEOG7P2XV4.
PhylomeDBP16860.
TreeFamTF106304.

Gene expression databases

BgeeP16860.
CleanExHS_NPPB.
GenevestigatorP16860.

Family and domain databases

InterProIPR000663. Natr_peptide.
IPR002408. Natriuretic_peptide_brain.
[Graphical view]
PfamPF00212. ANP. 1 hit.
[Graphical view]
PRINTSPR00712. BNATPEPTIDE.
SMARTSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP16860.
GeneWikiBrain_natriuretic_peptide.
GenomeRNAi4879.
NextBio18780.
PMAP-CutDBP16860.
PROP16860.
SOURCESearch...

Entry information

Entry nameANFB_HUMAN
AccessionPrimary (citable) accession number: P16860
Secondary accession number(s): B0ZBE9, Q6FGY0, Q9P2Q7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 9, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM