Reviewed, UniProtKB/Swiss-Prot P16860 (ANFB_HUMAN)

Names and origin

Protein names

Recommended name:
    Natriuretic peptides B
Alternative name(s):
    Gamma-brain natriuretic peptide
Cleaved into the following 16 chains:
    1- Recommended name:
            Brain natriuretic peptide 32
                Short name=BNP-32
                Short name=BNP(1-32)
    2- Recommended name:
            BNP(1-30)
    3- Recommended name:
            BNP(1-29)
    4- Recommended name:
            BNP(1-28)
    5- Recommended name:
            BNP(2-31)
    6- Recommended name:
            BNP(3-32)
    7- Recommended name:
            BNP(3-30)
    8- Recommended name:
            BNP(3-29)
    9- Recommended name:
            BNP(4-32)
    10- Recommended name:
            BNP(4-31)
    11- Recommended name:
            BNP(4-30)
    12- Recommended name:
            BNP(4-29)
    13- Recommended name:
            BNP(4-27)
    14- Recommended name:
            BNP(5-32)
    15- Recommended name:
            BNP(5-31)
    16- Recommended name:
            BNP(5-29)

Gene names

Name:

NPPB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a cardiac hormone with a variety of biological actions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. Helps restore the body's salt and water balance. Improves heart function.
Subunit structure	Interacts with NPR3.
Subcellular location	Secreted.
Tissue specificity	Brain and also in atria, but at much lower levels than ANP.
Pharmaceutical use	Available under the name Nesiritide (Scios). Used for the treatment of heart failure.
Sequence similarities	Belongs to the natriuretic peptide family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Hormone Vasoactive
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing Pharmaceutical
Gene Ontology (GO)
Biological process	cell surface receptor linked signal transduction Non-traceable author statement. Source: UniProtKB diuresis Traceable author statement. Source: UniProtKB natriuresis Traceable author statement. Source: UniProtKB negative regulation of angiogenesis Traceable author statement. Source: UniProtKB negative regulation of cell growth Non-traceable author statement. Source: UniProtKB regulation of blood pressure Non-traceable author statement. Source: UniProtKB regulation of blood vessel size Inferred from electronic annotation. Source: UniProtKB-KW regulation of vascular permeability Traceable author statement. Source: UniProtKB regulation of vasodilation Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular space Non-traceable author statement. Source: UniProtKB
Molecular function	diuretic hormone activity Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.8
	Chain	27 – 134	108	Natriuretic peptides B		PRO_0000001531
	Peptide	103 – 134	32	Brain natriuretic peptide 32		PRO_0000001532
	Peptide	103 – 132	30	BNP(1-30)		PRO_0000364993
	Peptide	103 – 131	29	BNP(1-29)		PRO_0000364994
	Peptide	103 – 130	28	BNP(1-28)		PRO_0000364995
	Peptide	104 – 133	30	BNP(2-31)		PRO_0000364996
	Peptide	105 – 134	30	BNP(3-32)		PRO_0000364997
	Peptide	105 – 132	28	BNP(3-30)		PRO_0000364998
	Peptide	105 – 131	27	BNP(3-29)		PRO_0000364999
	Peptide	106 – 134	29	BNP(4-32)		PRO_0000365000
	Peptide	106 – 133	28	BNP(4-31)		PRO_0000365001
	Peptide	106 – 132	27	BNP(4-30)		PRO_0000365002
	Peptide	106 – 131	26	BNP(4-29)		PRO_0000365003
	Peptide	106 – 129	24	BNP(4-27)		PRO_0000365004
	Peptide	107 – 134	28	BNP(5-32)		PRO_0000365005
	Peptide	107 – 133	27	BNP(5-31)		PRO_0000365006
	Peptide	107 – 131	25	BNP(5-29)		PRO_0000365007
Amino acid modifications
	Disulfide bond	112 ↔ 128		Ref.11
Natural variations
	Natural variant	25	1	R → L: dbSNP rs5227.		VAR_014580
	Natural variant	47	1	R → H: dbSNP rs5229.		VAR_014581
	Natural variant	93	1	M → L: dbSNP rs5230.		VAR_014582

Sequences

	Sequence		Length	Mass (Da)
	P16860-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: DC884D9408462146	FASTA	134	14,726
	10 20 30 40 50 60 MDPQTAPSRA LLLLLFLHLA FLGGRSHPLG SPGSASDLET SGLQEQRNHL QGKLSELQVE 70 80 90 100 110 120 QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA PRSPKMVQGS GCFGRKMDRI 130 SSSSGLGCKV LRRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human and canine gene homologs of porcine brain natriuretic peptide." Seilhamer J.J., Arfsten A., Miller J.A., Lundquist P., Scarborough R.M., Lewicki J.A., Porter J.G. Biochem. Biophys. Res. Commun. 165:650-658(1989) [PubMed: 2597152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and sequence analysis of cDNA encoding a precursor for human brain natriuretic peptide." Sudoh T., Maekawa K., Kojima M., Minamino N., Kangawa K., Matsuo H. Biochem. Biophys. Res. Commun. 159:1427-1434(1989) [PubMed: 2522777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	NHLBI resequencing and genotyping service (RS&G) Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Spleen.
[8]	"Isolation and identification of human brain natriuretic peptides in cardiac atrium." Hino J., Tateyaa H., Minamino N., Kangawa K., Matsuo H. Biochem. Biophys. Res. Commun. 167:693-700(1990) [PubMed: 2138890] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-58 AND 103-134.
[9]	"Isolation and sequence determination of human brain natriuretic peptide in human atrium." Kambayashi Y., Nakao K., Mukoyama M., Saito Y., Ogawa Y., Shiono S., Inouye K., Yoshida N., Imura H. FEBS Lett. 259:341-345(1990) [PubMed: 2136732] [Abstract] Cited for: PROTEIN SEQUENCE OF 103-134.
[10]	"Detection of endogenous B-type natriuretic peptide at very low concentrations in patients with heart failure." Niederkofler E.E., Kiernan U.A., O'Rear J., Menon S., Saghir S., Protter A.A., Nelson R.W., Schellenberger U. Circ. Heart Fail. 1:258-264(2008) Cited for: PROTEOLYTIC PROCESSING, MASS SPECTROMETRY.
[11]	"Structural determinants of natriuretic peptide receptor specificity and degeneracy." He X.-L., Dukkipati A., Garcia K.C. J. Mol. Biol. 361:698-714(2006) [PubMed: 16870210] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 111-131 IN COMPLEX WITH NPR3, DISULFIDE BOND.
+	Additional computationally mapped references.

Web resources

Wikipedia

Brain natriuretic peptide entry

Cross-references

Sequence databases

EMBLGenBankDDBJ

M31776 Genomic DNA. Translation: AAA35603.1.
M25296 mRNA. Translation: AAA36355.1.
CR541976 mRNA. Translation: CAG46774.1.
CR542003 mRNA. Translation: CAG46800.1.
EU326309 Genomic DNA. Translation: ACA05917.1.
AL021155 Genomic DNA. Translation: CAA15956.1.
CH471130 Genomic DNA. Translation: EAW71718.1.
BC025785 mRNA. Translation: AAH25785.1.

IPI

IPI00031101.

PIR

AWHUB. A36736.

RefSeq

NP_002512.1.

UniGene

Hs.219140

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YK1	X-ray	2.90	E	111-131	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P16860. 1 interaction.

Proteomic databases

PRIDE

P16860.

Genome annotation databases

Ensembl

ENSG00000120937. Homo sapiens. [Contig view]

GeneID

4879.

KEGG

hsa:4879.

Organism-specific databases

GeneCards

GC01M011851.

H-InvDB

HIX0000131.

HGNC

HGNC:7940. NPPB.

MIM

600295. gene.

PharmGKB

PA31734.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P16860.

HOVERGEN

P16860.

OMA

P16860. GCNVLRR.

Gene expression databases

ArrayExpress

P16860.

Bgee

P16860.

CleanEx

HS_NPPB.

GermOnline

ENSG00000120937. Homo sapiens.

Family and domain databases

InterPro

IPR002408. Br__natriurtcpep.
IPR000663. Natr_peptide.
[Graphical view]

Pfam

PF00212. ANP. 1 hit.
[Graphical view]

PRINTS

PR00712. BNATPEPTIDE.

ProDom

PD005617. Natr_peptide. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00183. NAT_PEP. 1 hit.
[Graphical view]

PROSITE

PS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB01136. Carvedilol.
DB04899. Nesiritide.
DB00624. Testosterone.

NextBio

18780.

PMAP-CutDB

P16860.

SOURCE

Search...

Entry information

Entry name

ANFB_HUMAN

Accession

Primary (citable) accession number: P16860
Secondary accession number(s): B0ZBE9, Q6FGY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)