Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P16860

- ANFB_HUMAN

UniProt

P16860 - ANFB_HUMAN

Protein

Natriuretic peptides B

Gene

NPPB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.1 Publication

    GO - Molecular functioni

    1. diuretic hormone activity Source: UniProtKB
    2. hormone activity Source: UniProtKB
    3. receptor binding Source: UniProtKB

    GO - Biological processi

    1. body fluid secretion Source: UniProtKB
    2. cell surface receptor signaling pathway Source: UniProtKB
    3. cGMP biosynthetic process Source: UniProtKB
    4. negative regulation of angiogenesis Source: UniProtKB
    5. negative regulation of cell growth Source: UniProtKB
    6. positive regulation of renal sodium excretion Source: UniProtKB
    7. positive regulation of urine volume Source: UniProtKB
    8. receptor guanylyl cyclase signaling pathway Source: UniProtKB
    9. regulation of blood pressure Source: UniProtKB
    10. regulation of blood vessel size Source: UniProtKB-KW
    11. regulation of vascular permeability Source: UniProtKB
    12. regulation of vasodilation Source: UniProtKB

    Keywords - Molecular functioni

    Hormone, Vasoactive

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Natriuretic peptides B
    Alternative name(s):
    Gamma-brain natriuretic peptide
    Cleaved into the following 16 chains:
    Gene namesi
    Name:NPPB
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:7940. NPPB.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular space Source: UniProtKB
    3. protein complex Source: MGI

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Pharmaceutical usei

    Available under the name Nesiritide (Scios). Used for the treatment of heart failure.

    Organism-specific databases

    PharmGKBiPA31734.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 134108Natriuretic peptides BPRO_0000001531Add
    BLAST
    Peptidei103 – 13432Brain natriuretic peptide 32PRO_0000001532Add
    BLAST
    Peptidei103 – 13230BNP(1-30)PRO_0000364993Add
    BLAST
    Peptidei103 – 13129BNP(1-29)PRO_0000364994Add
    BLAST
    Peptidei103 – 13028BNP(1-28)PRO_0000364995Add
    BLAST
    Peptidei104 – 13330BNP(2-31)PRO_0000364996Add
    BLAST
    Peptidei105 – 13430BNP(3-32)PRO_0000364997Add
    BLAST
    Peptidei105 – 13228BNP(3-30)PRO_0000364998Add
    BLAST
    Peptidei105 – 13127BNP(3-29)PRO_0000364999Add
    BLAST
    Peptidei106 – 13429BNP(4-32)PRO_0000365000Add
    BLAST
    Peptidei106 – 13328BNP(4-31)PRO_0000365001Add
    BLAST
    Peptidei106 – 13227BNP(4-30)PRO_0000365002Add
    BLAST
    Peptidei106 – 13126BNP(4-29)PRO_0000365003Add
    BLAST
    Peptidei106 – 12924BNP(4-27)PRO_0000365004Add
    BLAST
    Peptidei107 – 13428BNP(5-32)PRO_0000365005Add
    BLAST
    Peptidei107 – 13327BNP(5-31)PRO_0000365006Add
    BLAST
    Peptidei107 – 13125BNP(5-29)PRO_0000365007Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi62 – 621O-linked (HexNAc...); Partial1 Publication
    Glycosylationi63 – 631O-linked (HexNAc...)1 Publication
    Glycosylationi70 – 701O-linked (HexNAc...)1 Publication
    Glycosylationi74 – 741O-linked (HexNAc...)1 Publication
    Glycosylationi79 – 791O-linked (HexNAc...)1 Publication
    Glycosylationi84 – 841O-linked (HexNAc...); Partial1 Publication
    Glycosylationi97 – 971O-linked (HexNAc...)1 Publication
    Disulfide bondi112 ↔ 1281 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP16860.
    PRIDEiP16860.

    PTM databases

    PhosphoSiteiP16860.

    Miscellaneous databases

    PMAP-CutDBP16860.

    Expressioni

    Tissue specificityi

    Brain and also in atria, but at much lower levels than ANP.

    Gene expression databases

    BgeeiP16860.
    CleanExiHS_NPPB.
    GenevestigatoriP16860.

    Interactioni

    Protein-protein interaction databases

    BioGridi110939. 6 interactions.
    IntActiP16860. 3 interactions.
    MINTiMINT-1434895.
    STRINGi9606.ENSP00000365651.

    Structurei

    Secondary structure

    1
    134
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi121 – 1233

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YK1X-ray2.90E111-131[»]
    3N56X-ray3.10C/D103-134[»]
    DisProtiDP00551.
    ProteinModelPortaliP16860.
    SMRiP16860. Positions 103-132.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP16860.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the natriuretic peptide family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG67899.
    HOGENOMiHOG000206568.
    HOVERGENiHBG004227.
    KOiK12335.
    OMAiSGCFGRK.
    OrthoDBiEOG7P2XV4.
    PhylomeDBiP16860.
    TreeFamiTF106304.

    Family and domain databases

    InterProiIPR000663. Natr_peptide.
    IPR002408. Natriuretic_peptide_brain.
    [Graphical view]
    PfamiPF00212. ANP. 1 hit.
    [Graphical view]
    PRINTSiPR00712. BNATPEPTIDE.
    SMARTiSM00183. NAT_PEP. 1 hit.
    [Graphical view]
    PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16860-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPQTAPSRA LLLLLFLHLA FLGGRSHPLG SPGSASDLET SGLQEQRNHL    50
    QGKLSELQVE QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA 100
    PRSPKMVQGS GCFGRKMDRI SSSSGLGCKV LRRH 134
    Length:134
    Mass (Da):14,726
    Last modified:August 1, 1990 - v1
    Checksum:iDC884D9408462146
    GO

    Sequence cautioni

    The sequence BAA90441.1 differs from that shown. Reason: Frameshift at position 44.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti25 – 251R → L.
    Corresponds to variant rs5227 [ dbSNP | Ensembl ].
    VAR_014580
    Natural varianti47 – 471R → H.
    Corresponds to variant rs5229 [ dbSNP | Ensembl ].
    VAR_014581
    Natural varianti93 – 931M → L.
    Corresponds to variant rs5230 [ dbSNP | Ensembl ].
    VAR_014582

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31776 Genomic DNA. Translation: AAA35603.1.
    M25296 mRNA. Translation: AAA36355.1.
    AB037521 Genomic DNA. Translation: BAA90441.1. Frameshift.
    CR541976 mRNA. Translation: CAG46774.1.
    CR542003 mRNA. Translation: CAG46800.1.
    EU326309 Genomic DNA. Translation: ACA05917.1.
    AL021155 Genomic DNA. Translation: CAA15956.1.
    CH471130 Genomic DNA. Translation: EAW71718.1.
    BC025785 mRNA. Translation: AAH25785.1.
    CCDSiCCDS140.1.
    PIRiA36736. AWHUB.
    RefSeqiNP_002512.1. NM_002521.2.
    UniGeneiHs.219140.

    Genome annotation databases

    EnsembliENST00000376468; ENSP00000365651; ENSG00000120937.
    GeneIDi4879.
    KEGGihsa:4879.
    UCSCiuc001atj.3. human.

    Polymorphism databases

    DMDMi113836.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Brain natriuretic peptide entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M31776 Genomic DNA. Translation: AAA35603.1 .
    M25296 mRNA. Translation: AAA36355.1 .
    AB037521 Genomic DNA. Translation: BAA90441.1 . Frameshift.
    CR541976 mRNA. Translation: CAG46774.1 .
    CR542003 mRNA. Translation: CAG46800.1 .
    EU326309 Genomic DNA. Translation: ACA05917.1 .
    AL021155 Genomic DNA. Translation: CAA15956.1 .
    CH471130 Genomic DNA. Translation: EAW71718.1 .
    BC025785 mRNA. Translation: AAH25785.1 .
    CCDSi CCDS140.1.
    PIRi A36736. AWHUB.
    RefSeqi NP_002512.1. NM_002521.2.
    UniGenei Hs.219140.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YK1 X-ray 2.90 E 111-131 [» ]
    3N56 X-ray 3.10 C/D 103-134 [» ]
    DisProti DP00551.
    ProteinModelPortali P16860.
    SMRi P16860. Positions 103-132.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110939. 6 interactions.
    IntActi P16860. 3 interactions.
    MINTi MINT-1434895.
    STRINGi 9606.ENSP00000365651.

    Chemistry

    DrugBanki DB01136. Carvedilol.

    PTM databases

    PhosphoSitei P16860.

    Polymorphism databases

    DMDMi 113836.

    Proteomic databases

    PaxDbi P16860.
    PRIDEi P16860.

    Protocols and materials databases

    DNASUi 4879.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000376468 ; ENSP00000365651 ; ENSG00000120937 .
    GeneIDi 4879.
    KEGGi hsa:4879.
    UCSCi uc001atj.3. human.

    Organism-specific databases

    CTDi 4879.
    GeneCardsi GC01M011918.
    HGNCi HGNC:7940. NPPB.
    MIMi 600295. gene.
    neXtProti NX_P16860.
    PharmGKBi PA31734.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG67899.
    HOGENOMi HOG000206568.
    HOVERGENi HBG004227.
    KOi K12335.
    OMAi SGCFGRK.
    OrthoDBi EOG7P2XV4.
    PhylomeDBi P16860.
    TreeFami TF106304.

    Miscellaneous databases

    EvolutionaryTracei P16860.
    GeneWikii Brain_natriuretic_peptide.
    GenomeRNAii 4879.
    NextBioi 18780.
    PMAP-CutDB P16860.
    PROi P16860.
    SOURCEi Search...

    Gene expression databases

    Bgeei P16860.
    CleanExi HS_NPPB.
    Genevestigatori P16860.

    Family and domain databases

    InterProi IPR000663. Natr_peptide.
    IPR002408. Natriuretic_peptide_brain.
    [Graphical view ]
    Pfami PF00212. ANP. 1 hit.
    [Graphical view ]
    PRINTSi PR00712. BNATPEPTIDE.
    SMARTi SM00183. NAT_PEP. 1 hit.
    [Graphical view ]
    PROSITEi PS00263. NATRIURETIC_PEPTIDE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cloning and sequence analysis of cDNA encoding a precursor for human brain natriuretic peptide."
      Sudoh T., Maekawa K., Kojima M., Minamino N., Kangawa K., Matsuo H.
      Biochem. Biophys. Res. Commun. 159:1427-1434(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Silver Project."
      Kitano T., Kobayakawa H., Saitou N.
      Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. NHLBI resequencing and genotyping service (RS&G)
      Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pancreas and Spleen.
    9. "Isolation and identification of human brain natriuretic peptides in cardiac atrium."
      Hino J., Tateyaa H., Minamino N., Kangawa K., Matsuo H.
      Biochem. Biophys. Res. Commun. 167:693-700(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-58 AND 103-134.
    10. "Isolation and sequence determination of human brain natriuretic peptide in human atrium."
      Kambayashi Y., Nakao K., Mukoyama M., Saito Y., Ogawa Y., Shiono S., Inouye K., Yoshida N., Imura H.
      FEBS Lett. 259:341-345(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 103-134.
    11. "Selective activation of the B natriuretic peptide receptor by C-type natriuretic peptide (CNP)."
      Koller K.J., Lowe D.G., Bennett G.L., Minamino N., Kangawa K., Matsuo H., Goeddel D.V.
      Science 252:120-123(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "The precursor to B-type natriuretic peptide is an O-linked glycoprotein."
      Schellenberger U., O'Rear J., Guzzetta A., Jue R.A., Protter A.A., Pollitt N.S.
      Arch. Biochem. Biophys. 451:160-166(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT THR-62; SER-63; SER-70; THR-74; SER-79; THR-84 AND THR-97.
    13. "Detection of endogenous B-type natriuretic peptide at very low concentrations in patients with heart failure."
      Niederkofler E.E., Kiernan U.A., O'Rear J., Menon S., Saghir S., Protter A.A., Nelson R.W., Schellenberger U.
      Circ. Heart Fail. 1:258-264(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING, IDENTIFICATION BY MASS SPECTROMETRY.
    14. "Structural determinants of natriuretic peptide receptor specificity and degeneracy."
      He X.-L., Dukkipati A., Garcia K.C.
      J. Mol. Biol. 361:698-714(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 111-131 IN COMPLEX WITH NPR3, DISULFIDE BOND.

    Entry informationi

    Entry nameiANFB_HUMAN
    AccessioniPrimary (citable) accession number: P16860
    Secondary accession number(s): B0ZBE9, Q6FGY0, Q9P2Q7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 147 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3