P16824 (DUT_HCMVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	DUT
Synonyms:	UL72

Organism

Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) [Complete proteome]

Taxonomic identifier

10360 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Betaherpesvirinae › Cytomegalovirus › Human cytomegalovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Subcellular location	Virion.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Virion
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000182965

Sequences

	Sequence		Length	Mass (Da)
	P16824 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: A747D7D4C1E80DA6	FASTA	388	43,575
	10 20 30 40 50 60 MLTMLTDRID SQLVLSRLPR SRFQRFWETP TLIMKEESAS SSGSIILAEK SVNMRYCVRF 70 80 90 100 110 120 ASDSDFQTTF TLPQSTEEKY DKEQHPGEDE ASSPLPSPLK VPYKWMPSSF IVKQCHTQLA 130 140 150 160 170 180 FYNKHIIWLS RERKVPTSLG VSLYIPEGFF GITFYKCLDA QFVCMPELLE SRLQVPQLDV 190 200 210 220 230 240 VNLNDTFQSI FPGTIEGDIG VFPCFVPEPW QLMNLPPPNE HRFFSLRTRQ TLVIGPGHTQ 250 260 270 280 290 300 TVYFDAAYVH APGICALIVG VRQFSQSDLI IRPTIWLPGT AAGVTVVNTS HTTVCISPHT 310 320 330 340 350 360 TVAKAVFTTH RFTYLPVGSH PLGQMIVPPT PDIGFTHTPE HALLQRTPSP VDDDVDETEE 370 380 DEKSSDAESP VNTNDVIFDV GPKPPRHP

References

[1]	"Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169." Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G. Curr. Top. Microbiol. Immunol. 154:125-169(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome." Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:17-28(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION.
[3]	Erratum Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:1053-1053(2003)
[4]	"Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome." Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., Shenk T., Smith R.D., Nelson J.A. J. Virol. 78:10960-10966(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[5]	Erratum Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., Shenk T., Smith R.D., Nelson J.A. J. Virol. 78:13395-13395(2004)

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17403 Genomic DNA. Translation: CAA35387.1. BK000394 Genomic DNA. Translation: DAA00168.1.
PIR	S09835.
3D structure databases
ProteinModelPortal	P16824.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
InterPro	IPR006882. Herpes_Orf11. [Graphical view]
Pfam	PF04797. Herpes_ORF11. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUT_HCMVA

Accession

Primary (citable) accession number: P16824
Secondary accession number(s): Q7M6M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program