Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine protein kinase UL97

Gene

UL97

Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase that plays important roles in several processes including nuclear viral egress, viral replication or regulation of host cell cycle progression. Participates in the acquisition of tegument during virion morphogenesis in the nucleus. Redistributes the host nuclear lamina by phosphorylating cellular Lamins-A/C. Plays a role in viral DNA synthesis by phosphorylating the DNA polymerase processivity factor UL44. Stimulates host cell cycle to support viral DNA synthesis by phosphorylating host retinoblastoma/RB1 protein. Additional substrates have been identified including host EF1D or H2B.9 Publications

Miscellaneous

Monophosphorylates and thereby activates the antiviral nucleoside analog ganciclovir (GCV) used to treat CMV infections. Subsequent di- and triphosphorylation are carried out by cellular enzymes. The viral DNA polymerase incorporates GCV triphosphate into the viral genome, leading to marked attenuation of viral DNA replication and successful suppression of the infection, while the uninfected cell does not have this ability because it lacks the viral kinase. Mutations in phosphotransferase may induce CMV resistance to antiviral therapies in immunocompromised patients.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei359ATPBy similarity1
Active sitei456Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi337 – 345ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processHost-virus interaction, Modulation of host cell cycle by virus
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine protein kinase UL97 (EC:2.7.1.-)
Alternative name(s):
Ganciclovir kinase
HSRF3 protein
Gene namesi
Name:UL97
OrganismiHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri10360 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008992 Componenti: Genome
  • UP000008991 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • virion Source: UniProtKB-SubCell

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881921 – 707Serine/threonine protein kinase UL97Add BLAST707

Post-translational modificationi

Autophosphorylates on serine and threonine residues.1 Publication

Proteomic databases

PRIDEiP16788.

Interactioni

Subunit structurei

Interacts with UL83.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP16788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 63Ala-richAdd BLAST23

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG09000040.

Family and domain databases

InterProiView protein in InterPro
IPR010615. Herpes_UL97.
IPR011009. Kinase-like_dom.
IPR008266. Tyr_kinase_AS.
PfamiView protein in Pfam
PF06734. UL97. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.

Sequencei

Sequence statusi: Complete.

P16788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSALRSRAR SASLGTTTQG WDPPPLRRPS RARRRQWMRE AAQAAAQAAV
60 70 80 90 100
QAAQAAAAQV AQAHVDENEV VDLMADEAGG GVTTLTTLSS VSTTTVLGHA
110 120 130 140 150
TFSACVRSDV MRDGEKEDAA SDKENLRRPV VPSTSSRGSA ASGDGYHGLR
160 170 180 190 200
CRETSAMWSF EYDRDGDVTS VRRALFTGGS DPSDSVSGVR GGRKRPLRPP
210 220 230 240 250
LVSLARTPLC RRRVGGVDAV LEENDVELRA ESQDSAVASG PGRIPQPLSG
260 270 280 290 300
SSGEESATAV EADSTSHDDV HCTCSNDQII TTSIRGLTCD PRMFLRLTHP
310 320 330 340 350
ELCELSISYL LVYVPKEDDF CHKICYAVDM SDESYRLGQG SFGEVWPLDR
360 370 380 390 400
YRVVKVARKH SETVLTVWMS GLIRTRAAGE QQQPPSLVGT GVHRGLLTAT
410 420 430 440 450
GCCLLHNVTV HRRFHTDMFH HDQWKLACID SYRRAFCTLA DAIKFLNHQC
460 470 480 490 500
RVCHFDITPM NVLIDVNPHN PSEIVRAALC DYSLSEPYPD YNERCVAVFQ
510 520 530 540 550
ETGTARRIPN CSHRLRECYH PAFRPMPLQK LLICDPHARF PVAGLRRYCM
560 570 580 590 600
SELSALGNVL GFCLMRLLDR RGLDEVRMGT EALLFKHAGA ACRALENGKL
610 620 630 640 650
THCSDACLLI LAAQMSYGAC LLGEHGAALV SHTLRFVEAK MSSCRVRAFR
660 670 680 690 700
RFYHECSQTM LHEYVRKNVE RLLATSDGLY LYNAFRRTTS IICEEDLDGD

CRQLFPE
Length:707
Mass (Da):78,233
Last modified:August 1, 1990 - v1
Checksum:i74914183E5A5E03A
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti19Q → E in strain: Isolate clinical E460, Isolate clinical E763 and Isolate clinical TH-7A. 1
Natural varianti68N → D in strain: Isolate clinical C005, Isolate clinical C076, Isolate clinical C128, Isolate clinical C325, Isolate clinical C327, Isolate clinical C336, Isolate clinical CL-1A, Isolate clinical E428, Isolate clinical E460, Isolate clinical E540, Isolate clinical E759, Isolate clinical E760, Isolate clinical E761, Isolate clinical E763, Isolate clinical FK-2A, Isolate clinical GR-3A, Isolate clinical HD-4A, Isolate clinical HR-7, Isolate clinical MG-8, Isolate clinical SN-11, Isolate clinical SW-12, Isolate clinical TH-7A and Isolate clinical TL-8A. 1
Natural varianti95T → S in strain: Isolate clinical C128. 1
Natural varianti108S → N in strain: Isolate clinical E460, Isolate clinical E763, Isolate clinical TH-7A and Isolate clinical TL-8A. 1
Natural varianti112R → C in strain: Isolate clinical C128. 1
Natural varianti126L → Q in strain: Isolate clinical C128, Isolate clinical C076, Isolate clinical CL-1A, Isolate clinical E428, Isolate clinical E760, Isolate clinical FK-2A, Isolate clinical HR-7, Isolate clinical MG-8, Isolate clinical SN-11 and Isolate clinical SW-12. 1
Natural varianti137R → C in strain: Isolate clinical HD-4A. 1
Natural varianti227E → D in strain: Isolate clinical TH-7A. 1
Natural varianti244I → V in strain: Isolate clinical C005, Isolate clinical C076, Isolate clinical C128, Isolate clinical C325, Isolate clinical C327, Isolate clinical C336, Isolate clinical CL-1A, Isolate clinical E428, Isolate clinical E460, Isolate clinical E540, Isolate clinical E759, Isolate clinical E760, Isolate clinical E761, Isolate clinical E763, Isolate clinical FK-2A, Isolate clinical GR-3A, Isolate clinical HD-4A, Isolate clinical HR-7, Isolate clinical MG-8, Isolate clinical SN-11, Isolate clinical SW-12, Isolate clinical TH-7A and Isolate clinical TL-8A. 1
Natural varianti449Q → K in strain: Isolate clinical C076. 1
Natural varianti460M → I in GCV resistant isolate. 1 Publication1
Natural varianti460M → V in GCV resistant isolate. 1 Publication1
Natural varianti469H → Y in strain: Isolate clinical C327 and Isolate clinical E761 and Isolate clinical FK-2A. 1
Natural varianti510N → S in strain: Isolate clinical HG-5A. 1
Natural varianti520H → Q in GCV resistant isolate. 1 Publication1
Natural varianti582A → T in strain: Isolate clinical PB-9. 1
Natural varianti592C → G in GCV resistant isolate. 1 Publication1
Natural varianti594A → P in GCV resistant isolate. 1 Publication1
Natural varianti594A → V in GCV resistant isolate. 1 Publication1
Natural varianti595L → S in GCV resistant isolate. 1 Publication1
Natural varianti595L → W in GCV resistant isolate. 1 Publication1
Natural varianti603C → W in GCV resistant isolate. 1
Natural varianti605D → E in strain: Isolate clinical C325. 1
Natural varianti607C → Y in GCV resistant isolate. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17403 Genomic DNA. Translation: CAA35333.1.
AF345348 Genomic DNA. Translation: AAL10763.1.
AF345349 Genomic DNA. Translation: AAL10764.1.
AF345350 Genomic DNA. Translation: AAL10765.1.
AF345351 Genomic DNA. Translation: AAL10766.1.
AF345352 Genomic DNA. Translation: AAL10767.1.
AF345353 Genomic DNA. Translation: AAL10768.1.
AF345354 Genomic DNA. Translation: AAL10769.1.
AF345355 Genomic DNA. Translation: AAL10770.1.
AF345356 Genomic DNA. Translation: AAL10771.1.
AF345357 Genomic DNA. Translation: AAL10772.1.
AF345358 Genomic DNA. Translation: AAL10773.1.
AF345359 Genomic DNA. Translation: AAL10774.1.
AF345360 Genomic DNA. Translation: AAL10775.1.
AF345361 Genomic DNA. Translation: AAL10776.1.
AF345362 Genomic DNA. Translation: AAL10777.1.
AF345363 Genomic DNA. Translation: AAL10778.1.
AF345364 Genomic DNA. Translation: AAL10779.1.
AF345365 Genomic DNA. Translation: AAL10780.1.
AF345366 Genomic DNA. Translation: AAL10781.1.
AF345367 Genomic DNA. Translation: AAL10782.1.
AF345368 Genomic DNA. Translation: AAL10783.1.
AF345369 Genomic DNA. Translation: AAL10784.1.
AF345370 Genomic DNA. Translation: AAL10785.1.
AF345371 Genomic DNA. Translation: AAL10786.1.
AF345372 Genomic DNA. Translation: AAL10787.1.
AF345373 Genomic DNA. Translation: AAL10788.1.
AF425081 Genomic DNA. Translation: AAL10789.1.
AF425082 Genomic DNA. Translation: AAL10790.1.
BK000394 Genomic DNA. Translation: DAA00194.1.
PIRiS09862. QQBEJ5.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17403 Genomic DNA. Translation: CAA35333.1.
AF345348 Genomic DNA. Translation: AAL10763.1.
AF345349 Genomic DNA. Translation: AAL10764.1.
AF345350 Genomic DNA. Translation: AAL10765.1.
AF345351 Genomic DNA. Translation: AAL10766.1.
AF345352 Genomic DNA. Translation: AAL10767.1.
AF345353 Genomic DNA. Translation: AAL10768.1.
AF345354 Genomic DNA. Translation: AAL10769.1.
AF345355 Genomic DNA. Translation: AAL10770.1.
AF345356 Genomic DNA. Translation: AAL10771.1.
AF345357 Genomic DNA. Translation: AAL10772.1.
AF345358 Genomic DNA. Translation: AAL10773.1.
AF345359 Genomic DNA. Translation: AAL10774.1.
AF345360 Genomic DNA. Translation: AAL10775.1.
AF345361 Genomic DNA. Translation: AAL10776.1.
AF345362 Genomic DNA. Translation: AAL10777.1.
AF345363 Genomic DNA. Translation: AAL10778.1.
AF345364 Genomic DNA. Translation: AAL10779.1.
AF345365 Genomic DNA. Translation: AAL10780.1.
AF345366 Genomic DNA. Translation: AAL10781.1.
AF345367 Genomic DNA. Translation: AAL10782.1.
AF345368 Genomic DNA. Translation: AAL10783.1.
AF345369 Genomic DNA. Translation: AAL10784.1.
AF345370 Genomic DNA. Translation: AAL10785.1.
AF345371 Genomic DNA. Translation: AAL10786.1.
AF345372 Genomic DNA. Translation: AAL10787.1.
AF345373 Genomic DNA. Translation: AAL10788.1.
AF425081 Genomic DNA. Translation: AAL10789.1.
AF425082 Genomic DNA. Translation: AAL10790.1.
BK000394 Genomic DNA. Translation: DAA00194.1.
PIRiS09862. QQBEJ5.

3D structure databases

ProteinModelPortaliP16788.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP16788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG09000040.

Family and domain databases

InterProiView protein in InterPro
IPR010615. Herpes_UL97.
IPR011009. Kinase-like_dom.
IPR008266. Tyr_kinase_AS.
PfamiView protein in Pfam
PF06734. UL97. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUL97_HCMVA
AccessioniPrimary (citable) accession number: P16788
Secondary accession number(s): Q7M6J4
, Q910D5, Q910F8, Q910W3, Q91B46, Q91B47, Q91B48, Q91B49, Q91B50, Q91B51, Q91B52, Q91B53, Q91B54, Q91B55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 10, 2017
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.