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P16785

- DEN_HCMVA

UniProt

P16785 - DEN_HCMVA

Protein

Deneddylase

Gene

UL48

Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei24 – 241PROSITE-ProRule annotation
    Active sitei160 – 1601PROSITE-ProRule annotation
    Active sitei162 – 1621PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Large tegument protein
    Short name:
    LTP
    Gene namesi
    Name:UL48
    OrganismiHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
    Taxonomic identifieri10360 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008991: Genome, UP000008992: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22412241DeneddylasePRO_0000116043Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP16785.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 226223Peptidase C76PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili544 – 57128Sequence AnalysisAdd
    BLAST
    Coiled coili765 – 79228Sequence AnalysisAdd
    BLAST
    Coiled coili1163 – 120038Sequence AnalysisAdd
    BLAST
    Coiled coili1240 – 127233Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi244 – 2474Poly-Thr
    Compositional biasi325 – 3284Poly-Ser
    Compositional biasi1921 – 193010Poly-Thr
    Compositional biasi2133 – 21375Poly-Ala

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16785-1 [UniParc]FASTAAdd to Basket

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    MKVTQASCHQ GDIARFGARA GNQCVCNGIM FLHALHLGGT SAVLQTEALD     50
    AIMEEGARLD ARLERELQKK LPAGGRLPVY RLGDEVPRRL ESRFGRTVHA 100
    LSRPFNGTTE TCDLDGYMCP GIFDFLRYAH AKPRPTYVLV TVNSLARAVV 150
    FTEDHMLVFD PHSSAECHNA AVYHCEGLHQ VLMVLTGFGV QLSPAFYYEA 200
    LFLYMLDVAT VPEAEIAARL VSTYRDRDID LTGVVRESAD TAATTTTAAP 250
    SLPPLPDPIV DPGCPPGVAP SIPVYDPSSS PKKTPEKRRK DLSGSKHGGK 300
    KKPPSTTSKT LATASSSPSA IAAASSSSAV PPSYSCGEGA LPALGRYQQL 350
    VDEVEQELKA LTLPPLPANT SAWTLHAAGT ESGANAATAT APSFDEAFLT 400
    DRLQQLIIHA VNQRSCLRRP CGPQSAAQQA VRAYLGLSKK LDAFLLNWLH 450
    HGLDLQRMHD YLSHKTTKGT YSTLDRALLE KMQVVFDPYG RQHGPALIAW 500
    VEEMLRYVES KPTNELSQRL QRFVTKRPMP VSDSFVCLRP VDFQRLTQVI 550
    EQRRRVLQRQ REEYHGVYEH LAGLITSIDI HDLDASDLNR REILKALQPL 600
    DDNAKQELFR LGNAKMLELQ MDLDRLSTQL LTRVHNHILN GFLPVEDLKQ 650
    MERVVEQVLR LFYDLRDLKL CDGSYEEGFV VIREQLSYLM TGTVRDNVPL 700
    LQEILQLRHA YQQATQQNEG RLTQIHDLLH VIETLVRDPG SRGSALTLAL 750
    VQEQLAQLEA LGGLQLPEVQ QRLQNAQLAL SRLYEEEEET QRFLDGLSYD 800
    DPPNEQTIKR HPQLREMLRR DEQTRLRLIN AVLSMFHTLV MRLARDESPR 850
    PTFFDAVSLL LQQLPPDSHE REDLRAANAT YAQMVKKLEQ IEKAGTGASE 900
    KRFQALRELV YFFRNHEYFF QHMVGRLGVG PQVTELYERY QHEMEEQHLE 950
    RLEREWQEEA GKLTVTSVED VQRVLARAPS HRVMHQMQQT LTTKMQDFLD 1000
    KEKRKQEEQQ RQLLDGYQKK VQQDLQRVVD AVKGEMLSTI PHQPLEATLE 1050
    LLLGLDQRAQ PLLDKFNQDL LSALQQLSKK LDGRINECLH GVLTGDVERR 1100
    CHPHREAAMQ TQASLNHLDQ ILGPQLLIHE TQQALQHAVH QAQFIEKCQQ 1150
    GDPTTAITGS EFEGDFARYR SSQQKMEEQL QETRQQMTET SERLDRSLRQ 1200
    DPGSSSVTRV PEKPFKGQEL AGRITPPPAD FQQPVFKTLL DQQADAARKA 1250
    LSDEADLLNQ KVQTQLRQRD EQLSTAQNLW TDLVTRHKMS GGLDVTTPDA 1300
    KALMEKPLET LRELLGKATQ QLPYLSAERT VRWMLAFLEE ALAQITADPT 1350
    HPHHGSRTHY RNLQQQAVES AVTLAHQIEQ NAACENFIAQ HQEATANGAS 1400
    TPRVDMVQAV EAVWQRLEPG RVAGGAARHQ KVQELLQRLG QTLGDLELQE 1450
    TLATEYFALL HGIQTFSYGL DFRSQLEKIR DLRTRFAELA KRRGTRLSNE 1500
    GVLPNPRKPQ ATTSLGAFTR GLNALERHVQ LGHQYLLNKL NGSSLVYRLE 1550
    DIPSVLPATH ETDPALIMRD RLRRLCFARH HDTFLEVVDV FGMRQIVTQA 1600
    GEPIHLVTDY GNVAFKYLAL RDDGRPLAWR RRCSGGGLKN VVTTRYKAIT 1650
    VAVAVCQTLR TFWPQISQYD LRPYLTQHQS HTHPAETHTL HNLKLFCYLV 1700
    STAWHQRIDT QQELTAADRV GSGEGGDVGE QRPGRGTVLR LSLQEFCVLI 1750
    AALYPEYIYT VLKYPVQMSL PSLTAHLHQD VIHAVVNNTH KMPPDHLPEQ 1800
    VKAFCITPTQ WPAMQLNKLF WENKLVQQLC QVGPQKSTPP LGKLWLYAMA 1850
    TLVFPQDMLQ CLWLELKPQY AETYASVSEL VQTLFQIFTQ QCEMVTEGYT 1900
    QPQLPTGEPV LQMIRVPRQD TTTTDTNTTT EPGLLDVFIQ TETALDYALG 1950
    SWLFGIPVCL GVHVADLLKG QRILVARHLE YTSRDRDFLR IQRSRDLNLS 2000
    QLLQDTWTET PLEHCWLQAQ IRRLRDYLRF PTRLEFIPLV IYNAQDHTVV 2050
    RVLRPPSTFE QDHSRLVLDE AFPTFPLYDQ DDNSSADNIA ASGAAPTPPV 2100
    PFNRVPVNIQ FLRENPPPIA RVQQPPRRHR HRAAAAADDD GQIDHVQDDT 2150
    SRTADSALVS TAFGGSVFQE NRLGETPLCR DELVAVAPGA ASTSFASPPI 2200
    TVLTQNVLSA LEILRLVRLD LRQLAQSVQD TIQHMRFLYL L 2241
    Length:2,241
    Mass (Da):253,223
    Last modified:August 1, 1990 - v1
    Checksum:iF88929E9F9D0458E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17403 Genomic DNA. Translation: CAA35407.1.
    BK000394 Genomic DNA. Translation: DAA00151.1.
    PIRiS09811.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17403 Genomic DNA. Translation: CAA35407.1 .
    BK000394 Genomic DNA. Translation: DAA00151.1 .
    PIRi S09811.

    3D structure databases

    ProteinModelPortali P16785.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169."
      Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.
      Curr. Top. Microbiol. Immunol. 154:125-169(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome."
      Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S.
      J. Gen. Virol. 84:17-28(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
    3. Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiDEN_HCMVA
    AccessioniPrimary (citable) accession number: P16785
    Secondary accession number(s): Q7M6N7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3