SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P16785

- DEN_HCMVA

UniProt

P16785 - DEN_HCMVA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Deneddylase

Gene
UL48
Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei24 – 241 By similarity
Active sitei160 – 1601 By similarity
Active sitei162 – 1621 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Large tegument protein
Short name:
LTP
Gene namesi
Name:UL48
OrganismiHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri10360 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000008991: Genome, UP000008992: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22412241DeneddylasePRO_0000116043Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Structurei

3D structure databases

ProteinModelPortaliP16785.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 226223Peptidase C76Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili544 – 57128 Reviewed predictionAdd
BLAST
Coiled coili765 – 79228 Reviewed predictionAdd
BLAST
Coiled coili1163 – 120038 Reviewed predictionAdd
BLAST
Coiled coili1240 – 127233 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi244 – 2474Poly-Thr
Compositional biasi325 – 3284Poly-Ser
Compositional biasi1921 – 193010Poly-Thr
Compositional biasi2133 – 21375Poly-Ala

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16785-1 [UniParc]FASTAAdd to Basket

« Hide

MKVTQASCHQ GDIARFGARA GNQCVCNGIM FLHALHLGGT SAVLQTEALD     50
AIMEEGARLD ARLERELQKK LPAGGRLPVY RLGDEVPRRL ESRFGRTVHA 100
LSRPFNGTTE TCDLDGYMCP GIFDFLRYAH AKPRPTYVLV TVNSLARAVV 150
FTEDHMLVFD PHSSAECHNA AVYHCEGLHQ VLMVLTGFGV QLSPAFYYEA 200
LFLYMLDVAT VPEAEIAARL VSTYRDRDID LTGVVRESAD TAATTTTAAP 250
SLPPLPDPIV DPGCPPGVAP SIPVYDPSSS PKKTPEKRRK DLSGSKHGGK 300
KKPPSTTSKT LATASSSPSA IAAASSSSAV PPSYSCGEGA LPALGRYQQL 350
VDEVEQELKA LTLPPLPANT SAWTLHAAGT ESGANAATAT APSFDEAFLT 400
DRLQQLIIHA VNQRSCLRRP CGPQSAAQQA VRAYLGLSKK LDAFLLNWLH 450
HGLDLQRMHD YLSHKTTKGT YSTLDRALLE KMQVVFDPYG RQHGPALIAW 500
VEEMLRYVES KPTNELSQRL QRFVTKRPMP VSDSFVCLRP VDFQRLTQVI 550
EQRRRVLQRQ REEYHGVYEH LAGLITSIDI HDLDASDLNR REILKALQPL 600
DDNAKQELFR LGNAKMLELQ MDLDRLSTQL LTRVHNHILN GFLPVEDLKQ 650
MERVVEQVLR LFYDLRDLKL CDGSYEEGFV VIREQLSYLM TGTVRDNVPL 700
LQEILQLRHA YQQATQQNEG RLTQIHDLLH VIETLVRDPG SRGSALTLAL 750
VQEQLAQLEA LGGLQLPEVQ QRLQNAQLAL SRLYEEEEET QRFLDGLSYD 800
DPPNEQTIKR HPQLREMLRR DEQTRLRLIN AVLSMFHTLV MRLARDESPR 850
PTFFDAVSLL LQQLPPDSHE REDLRAANAT YAQMVKKLEQ IEKAGTGASE 900
KRFQALRELV YFFRNHEYFF QHMVGRLGVG PQVTELYERY QHEMEEQHLE 950
RLEREWQEEA GKLTVTSVED VQRVLARAPS HRVMHQMQQT LTTKMQDFLD 1000
KEKRKQEEQQ RQLLDGYQKK VQQDLQRVVD AVKGEMLSTI PHQPLEATLE 1050
LLLGLDQRAQ PLLDKFNQDL LSALQQLSKK LDGRINECLH GVLTGDVERR 1100
CHPHREAAMQ TQASLNHLDQ ILGPQLLIHE TQQALQHAVH QAQFIEKCQQ 1150
GDPTTAITGS EFEGDFARYR SSQQKMEEQL QETRQQMTET SERLDRSLRQ 1200
DPGSSSVTRV PEKPFKGQEL AGRITPPPAD FQQPVFKTLL DQQADAARKA 1250
LSDEADLLNQ KVQTQLRQRD EQLSTAQNLW TDLVTRHKMS GGLDVTTPDA 1300
KALMEKPLET LRELLGKATQ QLPYLSAERT VRWMLAFLEE ALAQITADPT 1350
HPHHGSRTHY RNLQQQAVES AVTLAHQIEQ NAACENFIAQ HQEATANGAS 1400
TPRVDMVQAV EAVWQRLEPG RVAGGAARHQ KVQELLQRLG QTLGDLELQE 1450
TLATEYFALL HGIQTFSYGL DFRSQLEKIR DLRTRFAELA KRRGTRLSNE 1500
GVLPNPRKPQ ATTSLGAFTR GLNALERHVQ LGHQYLLNKL NGSSLVYRLE 1550
DIPSVLPATH ETDPALIMRD RLRRLCFARH HDTFLEVVDV FGMRQIVTQA 1600
GEPIHLVTDY GNVAFKYLAL RDDGRPLAWR RRCSGGGLKN VVTTRYKAIT 1650
VAVAVCQTLR TFWPQISQYD LRPYLTQHQS HTHPAETHTL HNLKLFCYLV 1700
STAWHQRIDT QQELTAADRV GSGEGGDVGE QRPGRGTVLR LSLQEFCVLI 1750
AALYPEYIYT VLKYPVQMSL PSLTAHLHQD VIHAVVNNTH KMPPDHLPEQ 1800
VKAFCITPTQ WPAMQLNKLF WENKLVQQLC QVGPQKSTPP LGKLWLYAMA 1850
TLVFPQDMLQ CLWLELKPQY AETYASVSEL VQTLFQIFTQ QCEMVTEGYT 1900
QPQLPTGEPV LQMIRVPRQD TTTTDTNTTT EPGLLDVFIQ TETALDYALG 1950
SWLFGIPVCL GVHVADLLKG QRILVARHLE YTSRDRDFLR IQRSRDLNLS 2000
QLLQDTWTET PLEHCWLQAQ IRRLRDYLRF PTRLEFIPLV IYNAQDHTVV 2050
RVLRPPSTFE QDHSRLVLDE AFPTFPLYDQ DDNSSADNIA ASGAAPTPPV 2100
PFNRVPVNIQ FLRENPPPIA RVQQPPRRHR HRAAAAADDD GQIDHVQDDT 2150
SRTADSALVS TAFGGSVFQE NRLGETPLCR DELVAVAPGA ASTSFASPPI 2200
TVLTQNVLSA LEILRLVRLD LRQLAQSVQD TIQHMRFLYL L 2241
Length:2,241
Mass (Da):253,223
Last modified:August 1, 1990 - v1
Checksum:iF88929E9F9D0458E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17403 Genomic DNA. Translation: CAA35407.1.
BK000394 Genomic DNA. Translation: DAA00151.1.
PIRiS09811.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17403 Genomic DNA. Translation: CAA35407.1 .
BK000394 Genomic DNA. Translation: DAA00151.1 .
PIRi S09811.

3D structure databases

ProteinModelPortali P16785.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169."
    Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.
    Curr. Top. Microbiol. Immunol. 154:125-169(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome."
    Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S.
    J. Gen. Virol. 84:17-28(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
  3. Cited for: IDENTIFICATION.

Entry informationi

Entry nameiDEN_HCMVA
AccessioniPrimary (citable) accession number: P16785
Secondary accession number(s): Q7M6N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi