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P16785 (DEN_HCMVA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Large tegument protein
Short name=LTP
Gene names
Name:UL48
OrganismHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) [Complete proteome]
Taxonomic identifier10360 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length2241 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 22412241Deneddylase
PRO_0000116043

Regions

Domain4 – 226223Peptidase C76
Coiled coil544 – 57128 Potential
Coiled coil765 – 79228 Potential
Coiled coil1163 – 120038 Potential
Coiled coil1240 – 127233 Potential
Compositional bias244 – 2474Poly-Thr
Compositional bias325 – 3284Poly-Ser
Compositional bias1921 – 193010Poly-Thr
Compositional bias2133 – 21375Poly-Ala

Sites

Active site241 By similarity
Active site1601 By similarity
Active site1621 By similarity

Sequences

Sequence LengthMass (Da)Tools
P16785 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: F88929E9F9D0458E

FASTA2,241253,223
        10         20         30         40         50         60 
MKVTQASCHQ GDIARFGARA GNQCVCNGIM FLHALHLGGT SAVLQTEALD AIMEEGARLD 

        70         80         90        100        110        120 
ARLERELQKK LPAGGRLPVY RLGDEVPRRL ESRFGRTVHA LSRPFNGTTE TCDLDGYMCP 

       130        140        150        160        170        180 
GIFDFLRYAH AKPRPTYVLV TVNSLARAVV FTEDHMLVFD PHSSAECHNA AVYHCEGLHQ 

       190        200        210        220        230        240 
VLMVLTGFGV QLSPAFYYEA LFLYMLDVAT VPEAEIAARL VSTYRDRDID LTGVVRESAD 

       250        260        270        280        290        300 
TAATTTTAAP SLPPLPDPIV DPGCPPGVAP SIPVYDPSSS PKKTPEKRRK DLSGSKHGGK 

       310        320        330        340        350        360 
KKPPSTTSKT LATASSSPSA IAAASSSSAV PPSYSCGEGA LPALGRYQQL VDEVEQELKA 

       370        380        390        400        410        420 
LTLPPLPANT SAWTLHAAGT ESGANAATAT APSFDEAFLT DRLQQLIIHA VNQRSCLRRP 

       430        440        450        460        470        480 
CGPQSAAQQA VRAYLGLSKK LDAFLLNWLH HGLDLQRMHD YLSHKTTKGT YSTLDRALLE 

       490        500        510        520        530        540 
KMQVVFDPYG RQHGPALIAW VEEMLRYVES KPTNELSQRL QRFVTKRPMP VSDSFVCLRP 

       550        560        570        580        590        600 
VDFQRLTQVI EQRRRVLQRQ REEYHGVYEH LAGLITSIDI HDLDASDLNR REILKALQPL 

       610        620        630        640        650        660 
DDNAKQELFR LGNAKMLELQ MDLDRLSTQL LTRVHNHILN GFLPVEDLKQ MERVVEQVLR 

       670        680        690        700        710        720 
LFYDLRDLKL CDGSYEEGFV VIREQLSYLM TGTVRDNVPL LQEILQLRHA YQQATQQNEG 

       730        740        750        760        770        780 
RLTQIHDLLH VIETLVRDPG SRGSALTLAL VQEQLAQLEA LGGLQLPEVQ QRLQNAQLAL 

       790        800        810        820        830        840 
SRLYEEEEET QRFLDGLSYD DPPNEQTIKR HPQLREMLRR DEQTRLRLIN AVLSMFHTLV 

       850        860        870        880        890        900 
MRLARDESPR PTFFDAVSLL LQQLPPDSHE REDLRAANAT YAQMVKKLEQ IEKAGTGASE 

       910        920        930        940        950        960 
KRFQALRELV YFFRNHEYFF QHMVGRLGVG PQVTELYERY QHEMEEQHLE RLEREWQEEA 

       970        980        990       1000       1010       1020 
GKLTVTSVED VQRVLARAPS HRVMHQMQQT LTTKMQDFLD KEKRKQEEQQ RQLLDGYQKK 

      1030       1040       1050       1060       1070       1080 
VQQDLQRVVD AVKGEMLSTI PHQPLEATLE LLLGLDQRAQ PLLDKFNQDL LSALQQLSKK 

      1090       1100       1110       1120       1130       1140 
LDGRINECLH GVLTGDVERR CHPHREAAMQ TQASLNHLDQ ILGPQLLIHE TQQALQHAVH 

      1150       1160       1170       1180       1190       1200 
QAQFIEKCQQ GDPTTAITGS EFEGDFARYR SSQQKMEEQL QETRQQMTET SERLDRSLRQ 

      1210       1220       1230       1240       1250       1260 
DPGSSSVTRV PEKPFKGQEL AGRITPPPAD FQQPVFKTLL DQQADAARKA LSDEADLLNQ 

      1270       1280       1290       1300       1310       1320 
KVQTQLRQRD EQLSTAQNLW TDLVTRHKMS GGLDVTTPDA KALMEKPLET LRELLGKATQ 

      1330       1340       1350       1360       1370       1380 
QLPYLSAERT VRWMLAFLEE ALAQITADPT HPHHGSRTHY RNLQQQAVES AVTLAHQIEQ 

      1390       1400       1410       1420       1430       1440 
NAACENFIAQ HQEATANGAS TPRVDMVQAV EAVWQRLEPG RVAGGAARHQ KVQELLQRLG 

      1450       1460       1470       1480       1490       1500 
QTLGDLELQE TLATEYFALL HGIQTFSYGL DFRSQLEKIR DLRTRFAELA KRRGTRLSNE 

      1510       1520       1530       1540       1550       1560 
GVLPNPRKPQ ATTSLGAFTR GLNALERHVQ LGHQYLLNKL NGSSLVYRLE DIPSVLPATH 

      1570       1580       1590       1600       1610       1620 
ETDPALIMRD RLRRLCFARH HDTFLEVVDV FGMRQIVTQA GEPIHLVTDY GNVAFKYLAL 

      1630       1640       1650       1660       1670       1680 
RDDGRPLAWR RRCSGGGLKN VVTTRYKAIT VAVAVCQTLR TFWPQISQYD LRPYLTQHQS 

      1690       1700       1710       1720       1730       1740 
HTHPAETHTL HNLKLFCYLV STAWHQRIDT QQELTAADRV GSGEGGDVGE QRPGRGTVLR 

      1750       1760       1770       1780       1790       1800 
LSLQEFCVLI AALYPEYIYT VLKYPVQMSL PSLTAHLHQD VIHAVVNNTH KMPPDHLPEQ 

      1810       1820       1830       1840       1850       1860 
VKAFCITPTQ WPAMQLNKLF WENKLVQQLC QVGPQKSTPP LGKLWLYAMA TLVFPQDMLQ 

      1870       1880       1890       1900       1910       1920 
CLWLELKPQY AETYASVSEL VQTLFQIFTQ QCEMVTEGYT QPQLPTGEPV LQMIRVPRQD 

      1930       1940       1950       1960       1970       1980 
TTTTDTNTTT EPGLLDVFIQ TETALDYALG SWLFGIPVCL GVHVADLLKG QRILVARHLE 

      1990       2000       2010       2020       2030       2040 
YTSRDRDFLR IQRSRDLNLS QLLQDTWTET PLEHCWLQAQ IRRLRDYLRF PTRLEFIPLV 

      2050       2060       2070       2080       2090       2100 
IYNAQDHTVV RVLRPPSTFE QDHSRLVLDE AFPTFPLYDQ DDNSSADNIA ASGAAPTPPV 

      2110       2120       2130       2140       2150       2160 
PFNRVPVNIQ FLRENPPPIA RVQQPPRRHR HRAAAAADDD GQIDHVQDDT SRTADSALVS 

      2170       2180       2190       2200       2210       2220 
TAFGGSVFQE NRLGETPLCR DELVAVAPGA ASTSFASPPI TVLTQNVLSA LEILRLVRLD 

      2230       2240 
LRQLAQSVQD TIQHMRFLYL L 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169."
Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.
Curr. Top. Microbiol. Immunol. 154:125-169(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome."
Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S.
J. Gen. Virol. 84:17-28(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
[3]Erratum
Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S.
J. Gen. Virol. 84:1053-1053(2003)
[4]"Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome."
Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., Shenk T., Smith R.D., Nelson J.A.
J. Virol. 78:10960-10966(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]Erratum
Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., Shenk T., Smith R.D., Nelson J.A.
J. Virol. 78:13395-13395(2004)

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17403 Genomic DNA. Translation: CAA35407.1.
BK000394 Genomic DNA. Translation: DAA00151.1.
PIRS09811.

3D structure databases

ProteinModelPortalP16785.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_HCMVA
AccessionPrimary (citable) accession number: P16785
Secondary accession number(s): Q7M6N7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries