P16782 (RRL_HCMVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase large subunit-like protein UL45

Gene names

Name:

UL45

Organism

Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5) [Complete proteome]

Taxonomic identifier

10360 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Betaherpesvirinae › Cytomegalovirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	906 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Does not have ribonucleotide reductase activity. Betaherpesviruses probably use another strategy to expand the dNTP pool in a quiescent host cell. Ref.4
Subcellular location	Virion Ref.4.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family.
Caution	Lacks the conserved sequence Asn-x-Cys-x-Glu essential for ribonucleotide reductase activity.

Ontologies

Keywords
Cellular component	Virion
Developmental stage	Late protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 906	906	Ribonucleoside-diphosphate reductase large subunit-like protein UL45		PRO_0000187235

Sequences

	Sequence		Length	Mass (Da)
	P16782 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: EE05E44B2DDEA6F9	FASTA	906	101,668
	10 20 30 40 50 60 MNPADADEEQ RVSSVPAHRC RPGRIPSRSA ETETEESSAE VAADTIGGDD SELEEGPLPG 70 80 90 100 110 120 GDKEASAGNT NVSSGVACVA GFTSGGGVVS WRPESPSPDG TPSVLSLTRD SGPAVPSRGG 130 140 150 160 170 180 RVSSGLSTFN PAGATRMELD SVEEEDDFGA SLCKVSPPIQ ATRMLMGKKC HCHGYWGKFR 190 200 210 220 230 240 FCGVQEPARE LPSDRNALWR EMDTVSRHSA GLGSFRLFQL IMRHGPCLIR HSPRCDLLLG 250 260 270 280 290 300 RFYFKANWAR ESRTPLCYAS ELCDESVRRF VLRHMEDLPK LAEETARFVE LAGCWGLYAA 310 320 330 340 350 360 ILCLDKVCRQ LHGQDESPGG VFLRIAVALT AAIENSRHSR IYRFHLDARF EGEVLESVLK 370 380 390 400 410 420 RCRDGQLSLS TFTMSTVGFD RVPQYDFLIS ADPFSRDASW AAMCKWMSTL SCGVSVSVNV 430 440 450 460 470 480 TRLNADVNSV IRCLGGYCDL IREKEVHRPV VRVFVDMWDV AAIRVINFIL KESTSELTGV 490 500 510 520 530 540 CYAFNVPSVL MKRYRAREQR YSLFGRPVSR RLSDLGQESA FEKEYSRCEQ SCPKVVVNTD 550 560 570 580 590 600 DFLKKMLLCA LKGRASVVFV HHVVKYSIMA DSVCLPPCLS PDMASCHFGE CDMPVQRLTV 610 620 630 640 650 660 NVARCVFARS DEQKLHLPDV VLGNTRRYFD LSVLRELVTE AVVWGNARLD ALMSASEWWV 670 680 690 700 710 720 ESALEKLRPL HIGVAGLHTA LMRLGFTYFA SWDLIERIFE HMYFAAVRAS VDLCKSGLPR 730 740 750 760 770 780 CEWFERTIYQ EGKFIFELYR LPRLSIASAR WEALRADMLE FGLRNCQFLA VGPDDEVAHL 790 800 810 820 830 840 WGVTPSVWAS RGTVFEEETV WSLCPPNREC YFPTVVRRPL RVPVVNYAWL EQHQEEGKAT 850 860 870 880 890 900 QCLFQAAPAI QNDVEMAAVN LSVFVDQCVA LVFYYDSGMT PDVLLARMLK WYHWRFKVGV YKYCAS

References

[1]	"Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169." Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G. Curr. Top. Microbiol. Immunol. 154:125-169(1990) [PubMed: 2161319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome." Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:17-28(2003) [PubMed: 12533697] [Abstract] Cited for: GENOME REANNOTATION.
[3]	Erratum Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S. J. Gen. Virol. 84:1053-1053(2003)
[4]	"The human cytomegalovirus UL45 gene product is a late, virion-associated protein and influences virus growth at low multiplicities of infection." Patrone M., Percivalle E., Secchi M., Fiorina L., Pedrali-Noy G., Zoppe M., Baldanti F., Hahn G., Koszinowski U.H., Milanesi G., Gallina A. J. Gen. Virol. 84:3359-3370(2003) [PubMed: 14645917] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PROBABLE ABSENCE OF RIBONUCLEOTIDE REDUCTASE ACTIVITY.
[5]	"Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome." Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., Shenk T., Smith R.D., Nelson J.A. J. Virol. 78:10960-10966(2004) [PubMed: 15452216] [Abstract] Cited for: IDENTIFICATION.
[6]	Erratum Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., Shenk T., Smith R.D., Nelson J.A. J. Virol. 78:13395-13395(2004)
[7]	"Tinkering with a viral ribonucleotide reductase." Lembo D., Brune W. Trends Biochem. Sci. 34:25-32(2009) [PubMed: 18990579] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17403 Genomic DNA. Translation: CAA35404.1. BK000394 Genomic DNA. Translation: DAA00148.1.
PIR	WMBEV3. S09808.
3D structure databases
ProteinModelPortal	P16782.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000788. Ribncl_red_lg_C. [Graphical view]
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
PROSITE	PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RRL_HCMVA

Accession

Primary (citable) accession number: P16782
Secondary accession number(s): Q7M6P0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	July 27, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program