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P16778

- VGLI_HCMVA

UniProt

P16778 - VGLI_HCMVA

Protein

UL37 immediate early glycoprotein

Gene

UL37

Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Isoform vMIA sequesters proapoptotic BAX at the outer mitochondrial membrane and prevents cytochrome c release and subsequent initiation of the proapoptotic cascade. Also provoques a calcium efflux from host endoplasmic reticulum and F-actin cytoskeleton disruption. Participates in the increase of host mitochondrial biogenesis, thus promoting viral replication by efficient use of newly made mitochondria.
    Isoform gpUL37 may play a role in escape from the host antiviral response.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei193 – 1942Cleavage site

    GO - Molecular functioni

    1. protein binding Source: UniProtKB

    GO - Biological processi

    1. modulation by virus of host apoptotic process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Modulation of host cell apoptosis by virus

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UL37 immediate early glycoprotein
    Gene namesi
    Name:UL37
    OrganismiHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
    Taxonomic identifieri10360 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008991: Genome, UP000008992: Genome

    Subcellular locationi

    Isoform gpUL37 : Host membrane; Single-pass membrane protein. Host endoplasmic reticulum membrane; Single-pass membrane protein. Host Golgi apparatus membrane; Single-pass membrane protein. Host mitochondrion membrane; Single-pass membrane protein
    Note: The C-terminal fragment localizes to the endoplasmic reticulum while the N-terminal fragment is stable and traffics to mitochondria.
    Isoform vMIA : Host mitochondrion membrane; Single-pass membrane protein. Host endoplasmic reticulum membrane; Single-pass membrane protein
    Note: Transported from the endoplasmic reticulum (ER) through the mitochondrial associated membrane (MAMs) to the mitochondrial outer membrane. Associates with internal lipid rafts (LRs) in the MAM.

    GO - Cellular componenti

    1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. host cell Golgi membrane Source: UniProtKB-SubCell
    3. host cell mitochondrial membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Host mitochondrion, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 487465UL37 immediate early glycoproteinPRO_0000037455Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi206 – 2061N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi210 – 2101N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi219 – 2191N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi223 – 2231N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi242 – 2421N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi246 – 2461N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi275 – 2751N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi281 – 2811N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi294 – 2941N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi306 – 3061N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi333 – 3331N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi337 – 3371N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi343 – 3431N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi379 – 3791N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi384 – 3841N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi391 – 3911N-linked (GlcNAc...); by hostSequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Isofom vMIA interacts with host BAX. Isoform vMIA interacts with host RSAD2/viperin; this interaction results in RSAD2/viperin relocalization from the endoplasmic reticulum to the mitochondria, actin cytoskeleton disruption and enhancement of infection.2 Publications

    Protein-protein interaction databases

    DIPiDIP-60096N.

    Structurei

    Secondary structure

    1
    487
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi138 – 1458
    Beta strandi146 – 1483

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LR1NMR-B130-150[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei433 – 45927HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi97 – 10812Poly-GluAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR010880. Herpes_UL37_HHV-5-rel.
    [Graphical view]
    PfamiPF07413. Herpes_UL37_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform gpUL37 (identifier: P16778-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSPVYVNLLG SVGLLAFWYF SYRWIQRKRL EDPLPPWLRK KKACALTRRS    50
    RHRLRRQHGV IDGENSETER SVDLVAALLA EAGEESVTED TEREDTEEER 100
    EDEEEENEAR TPEVNPIDAE GLSGLAREAC EALKKALRRH RFLWQRRQRA 150
    RMLQHNGPQQ SHHAAVFCRV HGLRGFQVSV WLLLTLLWST GHGVSVRCTY 200
    HGTDVNRTSN TTSMNCHLNC TRNHTQIYNG PCLGTEARLP LNVTFNQSRR 250
    KWHSVMLKFG FQYHLEGWFP LRVLNESREI NVTEVHGEVA CFRNDTNVTV 300
    GQLTLNFTGH SYVLRAIAHT SPFESYVRWE ETNVTDNATS SENTTTVMST 350
    LTKYAESDYI FLQDMCPRFL KRTVKLTRNK TKHNVTVTGN NMTTLPVWTP 400
    ECKGWTYWTT LSVMWRNRRS ALLRAKSRAL GHWALLSICT VAAGSIALLS 450
    LFCILLIGLR RDLLEDFRYI CRDEGSSSTK NDVHRIV 487
    Length:487
    Mass (Da):56,125
    Last modified:May 30, 2000 - v2
    Checksum:iFA39A37C51FFB4A3
    GO
    Isoform vMIA (identifier: P16778-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         163-163: H → Q
         164-487: Missing.

    Show »
    Length:163
    Mass (Da):19,115
    Checksum:iB657342BB57AEC21
    GO
    Isoform pUL37m (identifier: P16778-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         178-262: Missing.

    Show »
    Length:402
    Mass (Da):46,435
    Checksum:iBDFD81E69DC4753E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei163 – 1631H → Q in isoform vMIA. CuratedVSP_044014
    Alternative sequencei164 – 487324Missing in isoform vMIA. CuratedVSP_044015Add
    BLAST
    Alternative sequencei178 – 26285Missing in isoform pUL37m. CuratedVSP_044016Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17403 Genomic DNA. Translation: CAA35396.1.
    BK000394 Genomic DNA. Translation: DAA00142.1.
    PIRiS09801. QQBEU5.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17403 Genomic DNA. Translation: CAA35396.1 .
    BK000394 Genomic DNA. Translation: DAA00142.1 .
    PIRi S09801. QQBEU5.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LR1 NMR - B 130-150 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60096N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR010880. Herpes_UL37_HHV-5-rel.
    [Graphical view ]
    Pfami PF07413. Herpes_UL37_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An immediate early gene of human cytomegalovirus encodes a potential membrane glycoprotein."
      Kouzarides T., Bankier A.T., Satchwell S.C., Preddy E., Barrell B.G.
      Virology 165:151-164(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169."
      Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.
      Curr. Top. Microbiol. Immunol. 154:125-169(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ISOFORM PUL37M, ISOFORM VMIA.
    3. "The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome."
      Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J., McGeoch D.J., Hayward G.S.
      J. Gen. Virol. 84:17-28(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
    4. "The human cytomegalovirus UL37 immediate-early regulatory protein is an integral membrane N-glycoprotein which traffics through the endoplasmic reticulum and Golgi apparatus."
      Al-Barazi H.O., Colberg-Poley A.M.
      J. Virol. 70:7198-7208(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. Cited for: FUNCTION, INTERACTION WITH HOST BAX.
    6. "Cytomegalovirus cell death suppressor vMIA blocks Bax- but not Bak-mediated apoptosis by binding and sequestering Bax at mitochondria."
      Arnoult D., Bartle L.M., Skaletskaya A., Poncet D., Zamzami N., Park P.U., Sharpe J., Youle R.J., Goldmacher V.S.
      Proc. Natl. Acad. Sci. U.S.A. 101:7988-7993(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Internal cleavage of the human cytomegalovirus UL37 immediate-early glycoprotein and divergent trafficking of its proteolytic fragments."
      Mavinakere M.S., Colberg-Poley A.M.
      J. Gen. Virol. 85:1989-1994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE SITE, SUBCELLULAR LOCATION.
    8. "Trafficking of UL37 proteins into mitochondrion-associated membranes during permissive human cytomegalovirus infection."
      Bozidis P., Williamson C.D., Wong D.S., Colberg-Poley A.M.
      J. Virol. 84:7898-7903(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Human cytomegalovirus directly induces the antiviral protein viperin to enhance infectivity."
      Seo J.Y., Yaneva R., Hinson E.R., Cresswell P.
      Science 332:1093-1097(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST RSAD2.
    10. "Human cytomegalovirus infection increases mitochondrial biogenesis."
      Kaarbo M., Ager-Wick E., Osenbroch P.O., Kilander A., Skinnes R., Muller F., Eide L.
      Mitochondrion 11:935-945(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiVGLI_HCMVA
    AccessioniPrimary (citable) accession number: P16778
    Secondary accession number(s): P87887, Q7M6P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 59 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3