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Protein

Cysteine synthase B

Gene

cysM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product.

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase (cysE)
  2. Cysteine synthase B (cysM), Cysteine synthase A (cysK)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71Pyridoxal phosphate1 Publication1
Binding sitei255Pyridoxal phosphate1 Publication1

GO - Molecular functioni

  • cysteine synthase activity Source: EcoCyc
  • L-cysteine desulfhydrase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: GO_Central
  • transferase activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:ACSERLYB-MONOMER.
ECOL316407:JW2414-MONOMER.
MetaCyc:ACSERLYB-MONOMER.
BRENDAi2.5.1.47. 2026.
SABIO-RKP16703.
UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine synthase B (EC:2.5.1.47)
Short name:
CSase B
Alternative name(s):
O-acetylserine (thiol)-lyase B
Short name:
OAS-TL B
O-acetylserine sulfhydrylase B
Gene namesi
Name:cysM
Ordered Locus Names:b2421, JW2414
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10193. cysM.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001671081 – 303Cysteine synthase BAdd BLAST303

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41N6-(pyridoxal phosphate)lysine1

Proteomic databases

PaxDbiP16703.
PRIDEiP16703.

2D gel databases

SWISS-2DPAGEP16703.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4259326. 6 interactors.
DIPiDIP-9383N.
IntActiP16703. 21 interactors.
MINTiMINT-1300981.
STRINGi511145.b2421.

Structurei

Secondary structure

1303
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 7Combined sources4
Beta strandi13 – 15Combined sources3
Beta strandi17 – 20Combined sources4
Beta strandi22 – 24Combined sources3
Beta strandi26 – 31Combined sources6
Helixi32 – 34Combined sources3
Helixi41 – 54Combined sources14
Beta strandi63 – 67Combined sources5
Helixi71 – 83Combined sources13
Beta strandi86 – 92Combined sources7
Helixi97 – 105Combined sources9
Beta strandi109 – 113Combined sources5
Turni115 – 117Combined sources3
Helixi118 – 131Combined sources14
Beta strandi134 – 137Combined sources4
Turni140 – 142Combined sources3
Helixi145 – 152Combined sources8
Helixi154 – 161Combined sources8
Turni162 – 164Combined sources3
Beta strandi168 – 172Combined sources5
Beta strandi174 – 176Combined sources3
Helixi177 – 187Combined sources11
Beta strandi189 – 191Combined sources3
Beta strandi194 – 200Combined sources7
Helixi214 – 216Combined sources3
Helixi223 – 225Combined sources3
Beta strandi227 – 232Combined sources6
Helixi234 – 248Combined sources15
Helixi254 – 269Combined sources16
Beta strandi274 – 279Combined sources6
Beta strandi281 – 283Combined sources3
Helixi284 – 289Combined sources6
Turni290 – 292Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BHSX-ray2.67A/B/C/D1-303[»]
2BHTX-ray2.10A/B/C/D1-303[»]
2V03X-ray1.33A1-303[»]
ProteinModelPortaliP16703.
SMRiP16703.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16703.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 178Pyridoxal phosphate binding5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C6T. Bacteria.
COG0031. LUCA.
HOGENOMiHOG000217394.
InParanoidiP16703.
KOiK12339.
OMAiFTTTGPE.
PhylomeDBiP16703.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005858. CysM.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01136. cysKM. 1 hit.
TIGR01138. cysM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLEQTIGN TPLVKLQRMG PDNGSEVWLK LEGNNPAGSV KDRAALSMIV
60 70 80 90 100
EAEKRGEIKP GDVLIEATSG NTGIALAMIA ALKGYRMKLL MPDNMSQERR
110 120 130 140 150
AAMRAYGAEL ILVTKEQGME GARDLALEMA NRGEGKLLDQ FNNPDNPYAH
160 170 180 190 200
YTTTGPEIWQ QTGGRITHFV SSMGTTGTIT GVSRFMREQS KPVTIVGLQP
210 220 230 240 250
EEGSSIPGIR RWPTEYLPGI FNASLVDEVL DIHQRDAENT MRELAVREGI
260 270 280 290 300
FCGVSSGGAV AGALRVAKAN PDAVVVAIIC DRGDRYLSTG VFGEEHFSQG

AGI
Length:303
Mass (Da):32,664
Last modified:August 1, 1990 - v1
Checksum:i1C7FBCB943FCFE39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32101 Genomic DNA. Translation: AAA23640.1.
U00096 Genomic DNA. Translation: AAC75474.1.
AP009048 Genomic DNA. Translation: BAA16295.1.
PIRiD35402. SYECBC.
RefSeqiNP_416916.1. NC_000913.3.
WP_001336044.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75474; AAC75474; b2421.
BAA16295; BAA16295; BAA16295.
GeneIDi946888.
KEGGiecj:JW2414.
eco:b2421.
PATRICi32120227. VBIEscCol129921_2516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32101 Genomic DNA. Translation: AAA23640.1.
U00096 Genomic DNA. Translation: AAC75474.1.
AP009048 Genomic DNA. Translation: BAA16295.1.
PIRiD35402. SYECBC.
RefSeqiNP_416916.1. NC_000913.3.
WP_001336044.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BHSX-ray2.67A/B/C/D1-303[»]
2BHTX-ray2.10A/B/C/D1-303[»]
2V03X-ray1.33A1-303[»]
ProteinModelPortaliP16703.
SMRiP16703.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259326. 6 interactors.
DIPiDIP-9383N.
IntActiP16703. 21 interactors.
MINTiMINT-1300981.
STRINGi511145.b2421.

2D gel databases

SWISS-2DPAGEP16703.

Proteomic databases

PaxDbiP16703.
PRIDEiP16703.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75474; AAC75474; b2421.
BAA16295; BAA16295; BAA16295.
GeneIDi946888.
KEGGiecj:JW2414.
eco:b2421.
PATRICi32120227. VBIEscCol129921_2516.

Organism-specific databases

EchoBASEiEB0190.
EcoGeneiEG10193. cysM.

Phylogenomic databases

eggNOGiENOG4105C6T. Bacteria.
COG0031. LUCA.
HOGENOMiHOG000217394.
InParanoidiP16703.
KOiK12339.
OMAiFTTTGPE.
PhylomeDBiP16703.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.
BioCyciEcoCyc:ACSERLYB-MONOMER.
ECOL316407:JW2414-MONOMER.
MetaCyc:ACSERLYB-MONOMER.
BRENDAi2.5.1.47. 2026.
SABIO-RKP16703.

Miscellaneous databases

EvolutionaryTraceiP16703.
PROiP16703.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005858. CysM.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01136. cysKM. 1 hit.
TIGR01138. cysM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSM_ECOLI
AccessioniPrimary (citable) accession number: P16703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 2, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.