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Protein

Thiosulfate-binding protein

Gene

cysP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport.

GO - Molecular functioni

  • anion binding Source: EcoCyc
  • ATPase-coupled sulfate transmembrane transporter activity Source: InterPro
  • sulfur compound binding Source: InterPro

GO - Biological processi

  • sulfur compound metabolic process Source: EcoCyc

Keywordsi

Biological processSulfate transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CYSP-MONOMER.
MetaCyc:CYSP-MONOMER.

Protein family/group databases

TCDBi3.A.1.6.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiosulfate-binding protein
Gene namesi
Name:cysP
Ordered Locus Names:b2425, JW2418
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10195. cysP.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003168126 – 338Thiosulfate-binding proteinAdd BLAST313

Proteomic databases

EPDiP16700.
PaxDbiP16700.
PRIDEiP16700.

2D gel databases

SWISS-2DPAGEiP16700.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).Curated

Protein-protein interaction databases

BioGridi4263209. 14 interactors.
DIPiDIP-9384N.
IntActiP16700. 4 interactors.
STRINGi511145.b2425.

Structurei

3D structure databases

ProteinModelPortaliP16700.
SMRiP16700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108J2H. Bacteria.
COG4150. LUCA.
HOGENOMiHOG000260057.
InParanoidiP16700.
KOiK02048.
OMAiVDQSHAG.
PhylomeDBiP16700.

Family and domain databases

CDDicd01005. PBP2_CysP. 1 hit.
InterProiView protein in InterPro
IPR000957. Sulphate/thiosulphate-bd_CS.
IPR034408. Sulphate/thiosulphate_BS.
IPR005669. Thiosulph/SO4-bd.
PANTHERiPTHR30368. PTHR30368. 1 hit.
TIGRFAMsiTIGR00971. 3a0106s03. 1 hit.
PROSITEiView protein in PROSITE
PS00401. PROK_SULFATE_BIND_1. 1 hit.
PS00757. PROK_SULFATE_BIND_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVNLLKKNS LALVASLLLA GHVQATELLN SSYDVSRELF AALNPPFEQQ
60 70 80 90 100
WAKDNGGDKL TIKQSHAGSS KQALAILQGL KADVVTYNQV TDVQILHDKG
110 120 130 140 150
KLIPADWQSR LPNNSSPFYS TMGFLVRKGN PKNIHDWNDL VRSDVKLIFP
160 170 180 190 200
NPKTSGNARY TYLAAWGAAD KADGGDKGKT EQFMTQFLKN VEVFDTGGRG
210 220 230 240 250
ATTTFAERGL GDVLISFESE VNNIRKQYEA QGFEVVIPKT NILAEFPVAW
260 270 280 290 300
VDKNVQANGT EKAAKAYLNW LYSPQAQTII TDYYYRVNNP EVMDKLKDKF
310 320 330
PQTELFRVED KFGSWPEVMK THFTSGGELD KLLAAGRN
Length:338
Mass (Da):37,615
Last modified:August 1, 1990 - v1
Checksum:i79BCD531AFA01DC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32101 Genomic DNA. Translation: AAA23636.1.
U00096 Genomic DNA. Translation: AAC75478.1.
AP009048 Genomic DNA. Translation: BAA16299.1.
PIRiA35403. JGECT.
RefSeqiNP_416920.1. NC_000913.3.
WP_000290230.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75478; AAC75478; b2425.
BAA16299; BAA16299; BAA16299.
GeneIDi946883.
KEGGiecj:JW2418.
eco:b2425.
PATRICi32120235. VBIEscCol129921_2520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32101 Genomic DNA. Translation: AAA23636.1.
U00096 Genomic DNA. Translation: AAC75478.1.
AP009048 Genomic DNA. Translation: BAA16299.1.
PIRiA35403. JGECT.
RefSeqiNP_416920.1. NC_000913.3.
WP_000290230.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP16700.
SMRiP16700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263209. 14 interactors.
DIPiDIP-9384N.
IntActiP16700. 4 interactors.
STRINGi511145.b2425.

Protein family/group databases

TCDBi3.A.1.6.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEiP16700.

Proteomic databases

EPDiP16700.
PaxDbiP16700.
PRIDEiP16700.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75478; AAC75478; b2425.
BAA16299; BAA16299; BAA16299.
GeneIDi946883.
KEGGiecj:JW2418.
eco:b2425.
PATRICi32120235. VBIEscCol129921_2520.

Organism-specific databases

EchoBASEiEB0192.
EcoGeneiEG10195. cysP.

Phylogenomic databases

eggNOGiENOG4108J2H. Bacteria.
COG4150. LUCA.
HOGENOMiHOG000260057.
InParanoidiP16700.
KOiK02048.
OMAiVDQSHAG.
PhylomeDBiP16700.

Enzyme and pathway databases

BioCyciEcoCyc:CYSP-MONOMER.
MetaCyc:CYSP-MONOMER.

Miscellaneous databases

PROiPR:P16700.

Family and domain databases

CDDicd01005. PBP2_CysP. 1 hit.
InterProiView protein in InterPro
IPR000957. Sulphate/thiosulphate-bd_CS.
IPR034408. Sulphate/thiosulphate_BS.
IPR005669. Thiosulph/SO4-bd.
PANTHERiPTHR30368. PTHR30368. 1 hit.
TIGRFAMsiTIGR00971. 3a0106s03. 1 hit.
PROSITEiView protein in PROSITE
PS00401. PROK_SULFATE_BIND_1. 1 hit.
PS00757. PROK_SULFATE_BIND_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCYSP_ECOLI
AccessioniPrimary (citable) accession number: P16700
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 10, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.