Reviewed, UniProtKB/Swiss-Prot P16699 (MANB_BACSM)

Names and origin

Protein names	Recommended name:     Mannan endo-1,4-beta-mannosidase A and B     EC=3.2.1.78 Alternative name(s):     Beta-mannanase     Endo-1,4-mannanase Cleaved into the following 2 chains:     1- Recommended name:             Mannan endo-1,4-beta-mannosidase A     2- Recommended name:             Mannan endo-1,4-beta-mannosidase B
Organism	Bacillus sp. (strain AM-001)
Taxonomic identifier	1418 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Sequence similarities	Belongs to the glycosyl hydrolase 26 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	substituted mannan metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26
	Chain	27 – 513	487	Mannan endo-1,4-beta-mannosidase A		PRO_0000012169
	Chain	27 – 365	339	Mannan endo-1,4-beta-mannosidase B		PRO_0000012170

Sequences

	Sequence		Length	Mass (Da)
	P16699-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 88D105F622CDB5A8	FASTA	513	58,430
	10 20 30 40 50 60 MKVYKKVAFV MAFIMFFSVL PTISMSSEAN GAALSNPNAN QTTKNVYSWL ANLPNKSNKR 70 80 90 100 110 120 VVSGHFGGYS DSTLAWIKQC ARELTGKMPG ILSCDYKNWQ TRLYVADQIS YGCNQELINF 130 140 150 160 170 180 WNQGGLVTIS VHMPNPGFHS GENYKTILPT SQFQNLTNHR TTEGRRWKDM LDKMADGLDE 190 200 210 220 230 240 LQNNGVTVLF RPLHEMNGEW FWWGAEGYNQ FDQTRANAYI SAWRDMYQYF THERKLNNLI 250 260 270 280 290 300 WVYSPDVYRD HVTSYYPGAN YVDIVALDSY HPDPHSLTDQ YNRMIALDKP FAFAEIGPPE 310 320 330 340 350 360 SMAGSFDYSN YIQAIKQKYP RTVYFLAWND KWSPHNNRGA WDLFNDSWVV NRGEIDYGQS 370 380 390 400 410 420 NPATVLYDFE NNTLSWSGCE FTDGGPWTSN EWSANGTQSL KADVVLGNNS YHLQKTVNRN 430 440 450 460 470 480 LSSFKNLEIK VSHSSWGNVG SGMTARVFVK TGSAWRWNAG EFCQFAGKRT TALSIDLTKV 490 500 510 SNLHDVREIG VEYKAPANSN GKTAIYLDHV TVR

References

[1]	"Two Bacillus beta-mannanases having different COOH termini are produced in Escherichia coli carrying pMAH5." Akino T., Kato C., Horikoshi K. Appl. Environ. Microbiol. 55:3178-3183(1989) [PubMed: 2694961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M31797 Genomic DNA. Translation: AAA22586.1.
PIR	A37219.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH26. Glycoside Hydrolase Family 26.
Family and domain databases
InterPro	IPR000805. Glyco_hydro_26. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02156. Glyco_hydro_26. 1 hit. [Graphical view]
PRINTS	PR00739. GLHYDRLASE26.
ProtoNet	Search...

Entry information

Entry name

MANB_BACSM

Accession

Primary (citable) accession number: P16699

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)