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Protein

Mannan endo-1,4-beta-mannosidase A and B

Gene
N/A
Organism
Bacillus mannanilyticus (strain DSM 16130 / JCM 10596 / AM-001)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could be involved in the degradation of glucomannan and catalyzes the endo hydrolysis of beta-1,4-linked mannan, galactomannan and glucomannan.By similarity

Miscellaneous

The production of the two beta-mannanases seems not to be caused by proteolytic cleavage.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei132SubstrateBy similarity1
Sitei194Plays an important role in maintaining the position of the catalytic nucleophileBy similarity1
Active sitei195Proton donorBy similarity1
Binding sitei200SubstrateBy similarity1
Binding sitei270SubstrateBy similarity1
Active sitei295NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiCBM59 Carbohydrate-Binding Module Family 59
GH26 Glycoside Hydrolase Family 26

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase A and B1 Publication (EC:3.2.1.78By similarity)
Alternative name(s):
1,4-beta-D-mannan mannanohydrolase1 Publication
Beta-mannanase1 Publication
Endo-1,4-mannanase1 Publication
Cleaved into the following 2 chains:
OrganismiBacillus mannanilyticus (strain DSM 16130 / JCM 10596 / AM-001)
Taxonomic identifieri1236954 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000001216927 – 513Mannan endo-1,4-beta-mannosidase AAdd BLAST487
ChainiPRO_000001217027 – 365Mannan endo-1,4-beta-mannosidase BAdd BLAST339

Proteomic databases

PRIDEiP16699

Structurei

3D structure databases

ProteinModelPortaliP16699
SMRiP16699
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 353GH26PROSITE-ProRule annotationAdd BLAST313

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni429 – 430Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 26 family.PROSITE-ProRule annotationCurated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR022790 GH26_dom
IPR000805 Glyco_hydro_26
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR40079 PTHR40079, 1 hit
PfamiView protein in Pfam
PF02156 Glyco_hydro_26, 1 hit
PRINTSiPR00739 GLHYDRLASE26
SUPFAMiSSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS51764 GH26, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVYKKVAFV MAFIMFFSVL PTISMSSEAN GAALSNPNAN QTTKNVYSWL
60 70 80 90 100
ANLPNKSNKR VVSGHFGGYS DSTLAWIKQC ARELTGKMPG ILSCDYKNWQ
110 120 130 140 150
TRLYVADQIS YGCNQELINF WNQGGLVTIS VHMPNPGFHS GENYKTILPT
160 170 180 190 200
SQFQNLTNHR TTEGRRWKDM LDKMADGLDE LQNNGVTVLF RPLHEMNGEW
210 220 230 240 250
FWWGAEGYNQ FDQTRANAYI SAWRDMYQYF THERKLNNLI WVYSPDVYRD
260 270 280 290 300
HVTSYYPGAN YVDIVALDSY HPDPHSLTDQ YNRMIALDKP FAFAEIGPPE
310 320 330 340 350
SMAGSFDYSN YIQAIKQKYP RTVYFLAWND KWSPHNNRGA WDLFNDSWVV
360 370 380 390 400
NRGEIDYGQS NPATVLYDFE NNTLSWSGCE FTDGGPWTSN EWSANGTQSL
410 420 430 440 450
KADVVLGNNS YHLQKTVNRN LSSFKNLEIK VSHSSWGNVG SGMTARVFVK
460 470 480 490 500
TGSAWRWNAG EFCQFAGKRT TALSIDLTKV SNLHDVREIG VEYKAPANSN
510
GKTAIYLDHV TVR
Length:513
Mass (Da):58,430
Last modified:August 1, 1990 - v1
Checksum:i88D105F622CDB5A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31797 Genomic DNA Translation: AAA22586.1
PIRiA37219

Entry informationi

Entry nameiMANB_BACMA
AccessioniPrimary (citable) accession number: P16699
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 23, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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