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Protein

Aminoalkylphosphonate N-acetyltransferase

Gene

phnO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Aminoalkylphosphonate N-acetyltransferase which is able to acetylate a range of aminoalkylphosphonic acids, including aminomethylphosphonate, (S)-1-aminoethylphosphonate and 2-aminoethyl- and 3-aminopropylphosphonate, using acetyl-CoA as acetyl donor. Is required for the utilization of aminomethylphosphonate and (S)-1-aminoethylphosphonate as a phosphate source via the C-P lyase pathway. Is also essential for the detoxification of (S)-1-aminoethylphosphonate, a structural analog of D-alanine that has bacteriocidal properties due to inhibition of cell wall synthesis.1 Publication

Catalytic activityi

Acetyl-CoA + aminomethylphosphonate = CoA + N-acetamidomethylphosphonate.1 Publication
Acetyl-CoA + (S)-1-aminoethylphosphonate = CoA + N-acetyl-(S)-1-aminoethylphosphonate.1 Publication

Cofactori

a divalent metal cationBy similarity

GO - Molecular functioni

  • N-acetyltransferase activity Source: EcoCyc
  • peptide alpha-N-acetyltransferase activity Source: GO_Central

GO - Biological processi

  • aminophosphonate metabolic process Source: EcoCyc
  • N-terminal protein amino acid acetylation Source: GO_Central
  • organic phosphonate transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG10724-MONOMER.
ECOL316407:JW4054-MONOMER.
MetaCyc:EG10724-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoalkylphosphonate N-acetyltransferase1 Publication (EC:2.3.1.-1 Publication)
Gene namesi
Name:phnO1 Publication
Ordered Locus Names:b4093, JW4054
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10724. phnO.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene have lost the ability to utilize aminomethylphosphonate or (S)-1-aminoethylphosphonate as the sole source of phosphate for growth, but are still able to utilize the other aminoalkylphosphonates 2-aminoethyl- and 3-aminopropylphosphonate, and alkylphosphonates. They are also much more susceptible to the bacteriocidal effect of (S)-1-aminoethylphosphonate, even with phosphate or D-alanine present.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 144144Aminoalkylphosphonate N-acetyltransferasePRO_0000058401Add
BLAST

Proteomic databases

PaxDbiP16691.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262023. 9 interactions.
IntActiP16691. 1 interaction.
STRINGi511145.b4093.

Structurei

3D structure databases

ProteinModelPortaliP16691.
SMRiP16691. Positions 43-137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 144141N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410849A. Bacteria.
ENOG410ZXJ8. LUCA.
HOGENOMiHOG000078524.
InParanoidiP16691.
KOiK09994.
OMAiGMIGLHM.
PhylomeDBiP16691.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPACELRPAT QYDTDAVYAL ICELKQAEFD HHAFRVGFNA NLRDPNMRYH
60 70 80 90 100
LALLDGEVVG MIGLHLQFHL HHVNWIGEIQ ELVVMPQARG LNVGSKLLAW
110 120 130 140
AEEEARQAGA EMTELSTNVK RHDAHRFYLR EGYEQSHFRF TKAL
Length:144
Mass (Da):16,570
Last modified:August 1, 1990 - v1
Checksum:iB4BCBD253788737B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05260 Genomic DNA. Translation: AAA24354.1.
D90227 Genomic DNA. Translation: BAA14275.1.
U14003 Genomic DNA. Translation: AAA96992.1.
U00096 Genomic DNA. Translation: AAC77054.1.
AP009048 Genomic DNA. Translation: BAE78096.1.
PIRiG35719.
RefSeqiNP_418517.1. NC_000913.3.
WP_001110490.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77054; AAC77054; b4093.
BAE78096; BAE78096; BAE78096.
GeneIDi948599.
KEGGiecj:JW4054.
eco:b4093.
PATRICi32123745. VBIEscCol129921_4221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05260 Genomic DNA. Translation: AAA24354.1.
D90227 Genomic DNA. Translation: BAA14275.1.
U14003 Genomic DNA. Translation: AAA96992.1.
U00096 Genomic DNA. Translation: AAC77054.1.
AP009048 Genomic DNA. Translation: BAE78096.1.
PIRiG35719.
RefSeqiNP_418517.1. NC_000913.3.
WP_001110490.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP16691.
SMRiP16691. Positions 43-137.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262023. 9 interactions.
IntActiP16691. 1 interaction.
STRINGi511145.b4093.

Proteomic databases

PaxDbiP16691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77054; AAC77054; b4093.
BAE78096; BAE78096; BAE78096.
GeneIDi948599.
KEGGiecj:JW4054.
eco:b4093.
PATRICi32123745. VBIEscCol129921_4221.

Organism-specific databases

EchoBASEiEB0718.
EcoGeneiEG10724. phnO.

Phylogenomic databases

eggNOGiENOG410849A. Bacteria.
ENOG410ZXJ8. LUCA.
HOGENOMiHOG000078524.
InParanoidiP16691.
KOiK09994.
OMAiGMIGLHM.
PhylomeDBiP16691.

Enzyme and pathway databases

BioCyciEcoCyc:EG10724-MONOMER.
ECOL316407:JW4054-MONOMER.
MetaCyc:EG10724-MONOMER.

Miscellaneous databases

PROiP16691.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHNO_ECOLI
AccessioniPrimary (citable) accession number: P16691
Secondary accession number(s): Q2M6L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The sequence shown is that of strains K12 and B.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.