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Protein

Aminoalkylphosphonate N-acetyltransferase

Gene

phnO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Aminoalkylphosphonate N-acetyltransferase which is able to acetylate a range of aminoalkylphosphonic acids, including aminomethylphosphonate, (S)-1-aminoethylphosphonate and 2-aminoethyl- and 3-aminopropylphosphonate, using acetyl-CoA as acetyl donor. Is required for the utilization of aminomethylphosphonate and (S)-1-aminoethylphosphonate as a phosphate source via the C-P lyase pathway. Is also essential for the detoxification of (S)-1-aminoethylphosphonate, a structural analog of D-alanine that has bacteriocidal properties due to inhibition of cell wall synthesis.1 Publication

Miscellaneous

The sequence shown is that of strains K12 and B.

Catalytic activityi

Acetyl-CoA + aminomethylphosphonate = CoA + N-acetamidomethylphosphonate.1 Publication
Acetyl-CoA + (S)-1-aminoethylphosphonate = CoA + N-acetyl-(S)-1-aminoethylphosphonate.1 Publication

Cofactori

a divalent metal cationBy similarity

GO - Molecular functioni

  • N-acetyltransferase activity Source: EcoCyc

GO - Biological processi

  • aminophosphonate metabolic process Source: EcoCyc

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG10724-MONOMER
MetaCyc:EG10724-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoalkylphosphonate N-acetyltransferase1 Publication (EC:2.3.1.-1 Publication)
Gene namesi
Name:phnO1 Publication
Ordered Locus Names:b4093, JW4054
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10724 phnO

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene have lost the ability to utilize aminomethylphosphonate or (S)-1-aminoethylphosphonate as the sole source of phosphate for growth, but are still able to utilize the other aminoalkylphosphonates 2-aminoethyl- and 3-aminopropylphosphonate, and alkylphosphonates. They are also much more susceptible to the bacteriocidal effect of (S)-1-aminoethylphosphonate, even with phosphate or D-alanine present.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584011 – 144Aminoalkylphosphonate N-acetyltransferaseAdd BLAST144

Proteomic databases

PaxDbiP16691
PRIDEiP16691

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4262023, 11 interactors
IntActiP16691, 1 interactor
STRINGi316385.ECDH10B_4284

Structurei

3D structure databases

ProteinModelPortaliP16691
SMRiP16691
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 144N-acetyltransferasePROSITE-ProRule annotationAdd BLAST141

Phylogenomic databases

eggNOGiENOG410849A Bacteria
ENOG410ZXJ8 LUCA
HOGENOMiHOG000078524
InParanoidiP16691
KOiK09994
OMAiKRIRAHQ
PhylomeDBiP16691

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR000182 GNAT_dom
PfamiView protein in Pfam
PF00583 Acetyltransf_1, 1 hit
SUPFAMiSSF55729 SSF55729, 1 hit
PROSITEiView protein in PROSITE
PS51186 GNAT, 1 hit

Sequencei

Sequence statusi: Complete.

P16691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPACELRPAT QYDTDAVYAL ICELKQAEFD HHAFRVGFNA NLRDPNMRYH
60 70 80 90 100
LALLDGEVVG MIGLHLQFHL HHVNWIGEIQ ELVVMPQARG LNVGSKLLAW
110 120 130 140
AEEEARQAGA EMTELSTNVK RHDAHRFYLR EGYEQSHFRF TKAL
Length:144
Mass (Da):16,570
Last modified:August 1, 1990 - v1
Checksum:iB4BCBD253788737B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05260 Genomic DNA Translation: AAA24354.1
D90227 Genomic DNA Translation: BAA14275.1
U14003 Genomic DNA Translation: AAA96992.1
U00096 Genomic DNA Translation: AAC77054.1
AP009048 Genomic DNA Translation: BAE78096.1
PIRiG35719
RefSeqiNP_418517.1, NC_000913.3
WP_001110490.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77054; AAC77054; b4093
BAE78096; BAE78096; BAE78096
GeneIDi948599
KEGGiecj:JW4054
eco:b4093
PATRICifig|1411691.4.peg.2607

Similar proteinsi

Entry informationi

Entry nameiPHNO_ECOLI
AccessioniPrimary (citable) accession number: P16691
Secondary accession number(s): Q2M6L0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health