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Protein

Ribose 1,5-bisphosphate phosphokinase PhnN

Gene

phnN

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of ribose 1,5-bisphosphate to 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP). Accepts ATP but not GTP as a phosphoryl donor, and uses ribose 1,5-bisphosphate but not ribose, ribose 1-phosphate, or ribose 5-phosphate as a phosphoryl acceptor.2 Publications

Catalytic activityi

ATP + alpha-D-ribose 1,5-bisphosphate = ADP + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Phosphopentomutase (deoB)
  2. no protein annotated in this organism
  3. Ribose 1,5-bisphosphate phosphokinase PhnN (phnN)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route II), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 17ATPBy similarity8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ribose 1,5-bisphosphate phosphokinase activity Source: UniProtKB

GO - Biological processi

  • 5-phosphoribose 1-diphosphate biosynthetic process Source: UniProtKB
  • organic phosphonate metabolic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10723-MONOMER
MetaCyc:EG10723-MONOMER
UniPathwayiUPA00087; UER00175

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose 1,5-bisphosphate phosphokinase PhnN (EC:2.7.4.23)
Alternative name(s):
Ribose 1,5-bisphosphokinase
Gene namesi
Name:phnN
Ordered Locus Names:b4094, JW4055
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10723 phnN

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584001 – 185Ribose 1,5-bisphosphate phosphokinase PhnNAdd BLAST185

Proteomic databases

PaxDbiP16690
PRIDEiP16690

Interactioni

Protein-protein interaction databases

BioGridi4262022, 2 interactors
IntActiP16690, 33 interactors
STRINGi316385.ECDH10B_4285

Structurei

3D structure databases

ProteinModelPortaliP16690
SMRiP16690
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribose 1,5-bisphosphokinase family.Curated

Phylogenomic databases

eggNOGiENOG4105MFQ Bacteria
COG3709 LUCA
HOGENOMiHOG000037637
InParanoidiP16690
KOiK05774
OMAiMARLIWL
PhylomeDBiP16690

Family and domain databases

HAMAPiMF_00836 PhnN, 1 hit
InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR027417 P-loop_NTPase
IPR012699 PhnN
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02322 phosphon_PhnN, 1 hit

Sequencei

Sequence statusi: Complete.

P16690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMGKLIWLMG PSGSGKDSLL AELRLREQTQ LLVAHRYITR DASAGSENHI
60 70 80 90 100
ALSEQEFFTR AGQNLLALSW HANGLYYGVG VEIDLWLHAG FDVLVNGSRA
110 120 130 140 150
HLPQARARYQ SALLPVCLQV SPEILRQRLE NRGRENASEI NARLARAARY
160 170 180
TPQDCHTLNN DGSLRQSVDT LLTLIHQKEK HHACL
Length:185
Mass (Da):20,730
Last modified:August 1, 1990 - v1
Checksum:i399ADE57C7975325
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05260 Genomic DNA Translation: AAA24353.1
D90227 Genomic DNA Translation: BAA14274.1
U14003 Genomic DNA Translation: AAA96993.1
U00096 Genomic DNA Translation: AAC77055.1
AP009048 Genomic DNA Translation: BAE78097.1
PIRiF35719
RefSeqiNP_418518.1, NC_000913.3
WP_000971886.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77055; AAC77055; b4094
BAE78097; BAE78097; BAE78097
GeneIDi948608
KEGGiecj:JW4055
eco:b4094
PATRICifig|1411691.4.peg.2606

Similar proteinsi

Entry informationi

Entry nameiPHNN_ECOLI
AccessioniPrimary (citable) accession number: P16690
Secondary accession number(s): Q2M6K9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health