P16689 (PHNM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase
Short name=RPnTP diphosphatase
EC=3.6.1.63

Gene names

Name:	phnM
Ordered Locus Names:	b4095, JW4056

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydrolysis of alpha-D-ribose 1-methylphosphonate triphosphate (RPnTP) to form alpha-D-ribose 1-methylphosphonate phosphate (PRPn) and diphosphate. Ref.6
Catalytic activity	Alpha-D-ribose 1-methylphosphonate 5-triphosphate + H2O = alpha-D-ribose 1-methylphosphonate 5-phosphate + diphosphate. Ref.6
Miscellaneous	The sequence shown is that of strain K12.
Biophysicochemical properties	Kinetic parameters: KM=56 µM for alpha-D-ribose 1-methylphosphonate 5-triphosphate Ref.6 KM=98 µM for D-ribose-5-triphosphate KM=200 µM for D-ribose-5-diphosphate

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	organic phosphonate catabolic process Inferred from mutant phenotype PubMed 8388873. Source: EcoCyc
Molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase		PRO_0000058399
Natural variations
	Natural variant	318	1	Q → E in strain: B.

Sequences

	Sequence		Length	Mass (Da)
	P16689 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 28CC9C5C77EAD37D	FASTA	378	42,010
	10 20 30 40 50 60 MIINNVKLVL ENEVVSGSLE VQNGEIRAFA ESQSRLPEAM DGEGGWLLPG LIELHTDNLD 70 80 90 100 110 120 KFFTPRPKVD WPAHSAMSSH DALMVASGIT TVLDAVAIGD VRDGGDRLEN LEKMINAIEE 130 140 150 160 170 180 TQKRGVNRAE HRLHLRCELP HHTTLPLFEK LVQREPVTLV SLMDHSPGQR QFANREKYRE 190 200 210 220 230 240 YYQGKYSLTD AQMQQYEEEQ LALAARWSQP NRESIAALCR ARKIALASHD DATHAHVAES 250 260 270 280 290 300 HQLGSVIAEF PTTFEAAEAS RKHGMNVLMG APNIVRGGSH SGNVAASELA QLGLLDILSS 310 320 330 340 350 360 DYYPASLLDA AFRVADDQSN RFTLPQAVKL VTKNPAQALN LQDRGVIGEG KRADLVLAHR 370 KDNHIHIDHV WRQGKRVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B." Chen C.-M., Ye Q.-Z., Zhu Z., Wanner B.L., Walsh C.T. J. Biol. Chem. 265:4461-4471(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B.
[2]	"Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12." Makino K., Kim S.K., Shinagawa H., Amemura M., Nakata A. J. Bacteriol. 173:2665-2672(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Intermediates in the transformation of phosphonates to phosphate by bacteria." Kamat S.S., Williams H.J., Raushel F.M. Nature 480:570-573(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05260 Genomic DNA. Translation: AAA24352.1. D90227 Genomic DNA. Translation: BAA14273.1. U14003 Genomic DNA. Translation: AAA96994.1. U00096 Genomic DNA. Translation: AAC77056.1. AP009048 Genomic DNA. Translation: BAE78098.1.
PIR	S56323.
RefSeq	NP_418519.1. NC_000913.2. YP_492239.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P16689.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b4095.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77056; AAC77056; b4095. BAE78098; BAE78098; BAE78098.
GeneID	12933027. 948613.
KEGG	ecj:Y75_p3983. eco:b4095.
PATRIC	32123749. VBIEscCol129921_4223.
Organism-specific databases
EchoBASE	EB0716.
EcoGene	EG10722. phnM.
Phylogenomic databases
eggNOG	COG3454.
HOGENOM	HOG000126996.
KO	K06162.
OMA	HDAQIVA.
ProtClustDB	PRK15446.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10722-MONOMER. ECOL316407:JW4056-MONOMER. MetaCyc:EG10722-MONOMER.
Gene expression databases
Genevestigator	P16689.
Family and domain databases
InterPro	IPR011059. Metal-dep_hydrolase_composite. IPR012696. Phosphono_PhnM. [Graphical view]
PIRSF	PIRSF038971. PhnM. 1 hit.
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR02318. phosphono_phnM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNM_ECOLI

Accession

Primary (citable) accession number: P16689
Secondary accession number(s): Q2M6K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program