Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase

Gene

phnM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of alpha-D-ribose 1-methylphosphonate triphosphate (RPnTP) to form alpha-D-ribose 1-methylphosphonate phosphate (PRPn) and diphosphate.1 Publication

Miscellaneous

The sequence shown is that of strain K12.

Catalytic activityi

Alpha-D-ribose 1-methylphosphonate 5-triphosphate + H2O = alpha-D-ribose 1-methylphosphonate 5-phosphate + diphosphate.1 Publication

Kineticsi

  1. KM=56 µM for alpha-D-ribose 1-methylphosphonate 5-triphosphate1 Publication
  2. KM=98 µM for D-ribose-5-triphosphate1 Publication
  3. KM=200 µM for D-ribose-5-diphosphate1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • organic phosphonate catabolic process Source: EcoCyc

    Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10722-MONOMER
    MetaCyc:EG10722-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (EC:3.6.1.63)
    Short name:
    RPnTP diphosphatase
    Gene namesi
    Name:phnM
    Ordered Locus Names:b4095, JW4056
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10722 phnM

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000583991 – 378Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphataseAdd BLAST378

    Proteomic databases

    PaxDbiP16689
    PRIDEiP16689

    Interactioni

    Protein-protein interaction databases

    BioGridi4262021, 11 interactors
    STRINGi316385.ECDH10B_4286

    Structurei

    3D structure databases

    ProteinModelPortaliP16689
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105FD8 Bacteria
    COG3454 LUCA
    HOGENOMiHOG000126996
    InParanoidiP16689
    KOiK06162
    OMAiMSLMDHT
    PhylomeDBiP16689

    Family and domain databases

    CDDicd01306 PhnM, 1 hit
    Gene3Di2.30.40.10, 2 hits
    InterProiView protein in InterPro
    IPR013108 Amidohydro_3
    IPR011059 Metal-dep_hydrolase_composite
    IPR032466 Metal_Hydrolase
    IPR012696 PhnM
    PfamiView protein in Pfam
    PF07969 Amidohydro_3, 1 hit
    PIRSFiPIRSF038971 PhnM, 1 hit
    SUPFAMiSSF51338 SSF51338, 2 hits
    SSF51556 SSF51556, 1 hit
    TIGRFAMsiTIGR02318 phosphono_phnM, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P16689-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MIINNVKLVL ENEVVSGSLE VQNGEIRAFA ESQSRLPEAM DGEGGWLLPG
    60 70 80 90 100
    LIELHTDNLD KFFTPRPKVD WPAHSAMSSH DALMVASGIT TVLDAVAIGD
    110 120 130 140 150
    VRDGGDRLEN LEKMINAIEE TQKRGVNRAE HRLHLRCELP HHTTLPLFEK
    160 170 180 190 200
    LVQREPVTLV SLMDHSPGQR QFANREKYRE YYQGKYSLTD AQMQQYEEEQ
    210 220 230 240 250
    LALAARWSQP NRESIAALCR ARKIALASHD DATHAHVAES HQLGSVIAEF
    260 270 280 290 300
    PTTFEAAEAS RKHGMNVLMG APNIVRGGSH SGNVAASELA QLGLLDILSS
    310 320 330 340 350
    DYYPASLLDA AFRVADDQSN RFTLPQAVKL VTKNPAQALN LQDRGVIGEG
    360 370
    KRADLVLAHR KDNHIHIDHV WRQGKRVF
    Length:378
    Mass (Da):42,010
    Last modified:November 1, 1991 - v2
    Checksum:i28CC9C5C77EAD37D
    GO

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural varianti318Q → E in strain: B. 1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J05260 Genomic DNA Translation: AAA24352.1
    D90227 Genomic DNA Translation: BAA14273.1
    U14003 Genomic DNA Translation: AAA96994.1
    U00096 Genomic DNA Translation: AAC77056.1
    AP009048 Genomic DNA Translation: BAE78098.1
    PIRiS56323
    RefSeqiNP_418519.1, NC_000913.3
    WP_000586325.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77056; AAC77056; b4095
    BAE78098; BAE78098; BAE78098
    GeneIDi948613
    KEGGiecj:JW4056
    eco:b4095
    PATRICifig|1411691.4.peg.2605

    Similar proteinsi

    Entry informationi

    Entry nameiPHNM_ECOLI
    AccessioniPrimary (citable) accession number: P16689
    Secondary accession number(s): Q2M6K8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: November 1, 1991
    Last modified: March 28, 2018
    This is version 136 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Cookie policy

    We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health