P16677 (PHNC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphonates import ATP-binding protein PhnC
EC=3.6.3.28

Gene names

Name:	phnC
Ordered Locus Names:	b4106, JW4067

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex PhnCDE involved in phosphonates, phosphate esters, phosphite and phosphate import. Responsible for energy coupling to the transport system. Ref.6 Ref.7
Catalytic activity	ATP + H2O + phosphonate(Out) = ADP + phosphate + phosphonate(In).
Subunit structure	The complex is composed of two ATP-binding proteins (PhnC), two transmembrane proteins (PhnE) and a solute-binding protein (PhnD) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Miscellaneous	Phosphonates utilization is cryptic in strain K12.
Sequence similarities	Belongs to the ABC transporter superfamily. Phosphonates importer (TC 3.A.1.9.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Phosphonate transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC alkylphosphonate transport Inferred from mutant phenotype Ref.7. Source: EcoCyc
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW organic phosphonate transmembrane-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Phosphonates import ATP-binding protein PhnC		PRO_0000092706
Regions
	Domain	5 – 253	249	ABC transporter
	Nucleotide binding	37 – 44	8	ATP Potential
Natural variations
	Natural variant	60	1	V → A in strain: B.
	Natural variant	87	1	H → N in strain: B.
	Natural variant	127	1	G → R in strain: B.
	Natural variant	255	1	V → I in strain: B.

Sequences

	Sequence		Length	Mass (Da)
	P16677 [UniParc]. Last modified November 1, 1991. Version 2. Checksum: 393EF8F30FD18D6C	FASTA	262	29,431
	10 20 30 40 50 60 MQTIIRVEKL AKTFNQHQAL HAVDLNIHHG EMVALLGPSG SGKSTLLRHL SGLITGDKSV 70 80 90 100 110 120 GSHIELLGRT VQREGRLARD IRKSRAHTGY IFQQFNLVNR LSVLENVLIG ALGSTPFWRT 130 140 150 160 170 180 CFSWFTGEQK QRALQALTRV GMVHFAHQRV STLSGGQQQR VAIARALMQQ AKVILADEPI 190 200 210 220 230 240 ASLDPESARI VMDTLRDINQ NDGITVVVTL HQVDYALRYC ERIVALRQGH VFYDGSSQQF 250 260 DNERFDHLYR SINRVEENAK AA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B." Chen C.-M., Ye Q.-Z., Zhu Z., Wanner B.L., Walsh C.T. J. Biol. Chem. 265:4461-4471(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B.
[2]	"Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12." Makino K., Kim S.K., Shinagawa H., Amemura M., Nakata A. J. Bacteriol. 173:2665-2672(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi." Metcalf W.W., Wanner B.L. J. Bacteriol. 173:587-600(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Mutational analysis of an Escherichia coli fourteen-gene operon for phosphonate degradation, using TnphoA' elements." Metcalf W.W., Wanner B.L. J. Bacteriol. 175:3430-3442(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05260 Genomic DNA. Translation: AAA24339.1. D90227 Genomic DNA. Translation: BAA14262.1. U14003 Genomic DNA. Translation: AAA97005.1. U00096 Genomic DNA. Translation: AAC77067.1. AP009048 Genomic DNA. Translation: BAE78108.1.
PIR	A65220.
RefSeq	NP_418530.1. NC_000913.2. YP_492249.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P16677.
SMR	P16677. Positions 2-256.
ModBase	Search...
Protein-protein interaction databases
IntAct	P16677. 1 interaction.
STRING	511145.b4106.
Protein family/group databases
TCDB	3.A.1.9.1. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PRIDE	P16677.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77067; AAC77067; b4106. BAE78108; BAE78108; BAE78108.
GeneID	12934096. 948623.
KEGG	ecj:Y75_p3993. eco:b4106.
PATRIC	32123771. VBIEscCol129921_4234.
Organism-specific databases
EchoBASE	EB0707.
EcoGene	EG10713. phnC.
Phylogenomic databases
eggNOG	COG3638.
KO	K02041.
OMA	DNDRFDH.
ProtClustDB	PRK09984.
Enzyme and pathway databases
BioCyc	EcoCyc:PHNC-MONOMER. ECOL316407:JW4067-MONOMER.
Gene expression databases
Genevestigator	P16677.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR012693. ABC_transpr_PhnC. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR02315. ABC_phnC. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51249. PHNC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHNC_ECOLI

Accession

Primary (citable) accession number: P16677
Secondary accession number(s): Q2M6J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program