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Protein

Sulfate/thiosulfate import ATP-binding protein CysA

Gene

cysA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the ABC transporter complex CysAWTP involved in sulfate/thiosulfate import. Responsible for energy coupling to the transport system.

Catalytic activityi

ATP + H2O + sulfate(Out) = ADP + phosphate + sulfate(In).UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi35 – 42ATPUniRule annotation8

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sulfate transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:CYSA-MONOMER.
ECOL316407:JW2415-MONOMER.
MetaCyc:CYSA-MONOMER.

Protein family/group databases

TCDBi3.A.1.6.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate/thiosulfate import ATP-binding protein CysAUniRule annotation (EC:3.6.3.25UniRule annotation)
Alternative name(s):
Sulfate-transporting ATPaseUniRule annotation
Gene namesi
Name:cysAUniRule annotation
Ordered Locus Names:b2422, JW2415
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10183. cysA.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000922651 – 365Sulfate/thiosulfate import ATP-binding protein CysAAdd BLAST365

Proteomic databases

PaxDbiP16676.
PRIDEiP16676.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).UniRule annotation

Protein-protein interaction databases

BioGridi4260755. 15 interactors.
DIPiDIP-9373N.
IntActiP16676. 4 interactors.
MINTiMINT-1267862.
STRINGi511145.b2422.

Structurei

3D structure databases

ProteinModelPortaliP16676.
SMRiP16676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 237ABC transporterUniRule annotationAdd BLAST235

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family. [View classification]UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IJ6. Bacteria.
COG1118. LUCA.
InParanoidiP16676.
KOiK02045.
OMAiHDDMHIT.
PhylomeDBiP16676.

Family and domain databases

CDDicd03296. ABC_CysA_sulfate_importer. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR014769. ABC_CysA_ATP-bd_C.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005666. Sulph_transpt1.
IPR024765. TOBE-like.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF12857. TOBE_3. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00968. 3a0106s01. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51237. CYSA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEIANIKK SFGRTQVLND ISLDIPSGQM VALLGPSGSG KTTLLRIIAG
60 70 80 90 100
LEHQTSGHIR FHGTDVSRLH ARDRKVGFVF QHYALFRHMT VFDNIAFGLT
110 120 130 140 150
VLPRRERPNA AAIKAKVTKL LEMVQLAHLA DRYPAQLSGG QKQRVALARA
160 170 180 190 200
LAVEPQILLL DEPFGALDAQ VRKELRRWLR QLHEELKFTS VFVTHDQEEA
210 220 230 240 250
TEVADRVVVM SQGNIEQADA PDQVWREPAT RFVLEFMGEV NRLQGTIRGG
260 270 280 290 300
QFHVGAHRWP LGYTPAYQGP VDLFLRPWEV DISRRTSLDS PLPVQVLEAS
310 320 330 340 350
PKGHYTQLVV QPLGWYNEPL TVVMHGDDAP QRGERLFVGL QHARLYNGDE
360
RIETRDEELA LAQSA
Length:365
Mass (Da):41,059
Last modified:November 1, 1997 - v2
Checksum:iB5FCCA346EDF2788
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136 – 137QL → HV in AAA23639 (PubMed:2188958).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32101 Genomic DNA. Translation: AAA23639.1.
U00096 Genomic DNA. Translation: AAC75475.1.
AP009048 Genomic DNA. Translation: BAA16296.1.
PIRiE65016. QRECSA.
RefSeqiNP_416917.1. NC_000913.3.
WP_000021036.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75475; AAC75475; b2422.
BAA16296; BAA16296; BAA16296.
GeneIDi946889.
KEGGiecj:JW2415.
eco:b2422.
PATRICi32120229. VBIEscCol129921_2517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32101 Genomic DNA. Translation: AAA23639.1.
U00096 Genomic DNA. Translation: AAC75475.1.
AP009048 Genomic DNA. Translation: BAA16296.1.
PIRiE65016. QRECSA.
RefSeqiNP_416917.1. NC_000913.3.
WP_000021036.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP16676.
SMRiP16676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260755. 15 interactors.
DIPiDIP-9373N.
IntActiP16676. 4 interactors.
MINTiMINT-1267862.
STRINGi511145.b2422.

Protein family/group databases

TCDBi3.A.1.6.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP16676.
PRIDEiP16676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75475; AAC75475; b2422.
BAA16296; BAA16296; BAA16296.
GeneIDi946889.
KEGGiecj:JW2415.
eco:b2422.
PATRICi32120229. VBIEscCol129921_2517.

Organism-specific databases

EchoBASEiEB0180.
EcoGeneiEG10183. cysA.

Phylogenomic databases

eggNOGiENOG4108IJ6. Bacteria.
COG1118. LUCA.
InParanoidiP16676.
KOiK02045.
OMAiHDDMHIT.
PhylomeDBiP16676.

Enzyme and pathway databases

BioCyciEcoCyc:CYSA-MONOMER.
ECOL316407:JW2415-MONOMER.
MetaCyc:CYSA-MONOMER.

Miscellaneous databases

PROiP16676.

Family and domain databases

CDDicd03296. ABC_CysA_sulfate_importer. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR014769. ABC_CysA_ATP-bd_C.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR008995. Mo/tungstate-bd_C_term_dom.
IPR027417. P-loop_NTPase.
IPR005666. Sulph_transpt1.
IPR024765. TOBE-like.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF12857. TOBE_3. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50331. SSF50331. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00968. 3a0106s01. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51237. CYSA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSA_ECOLI
AccessioniPrimary (citable) accession number: P16676
Secondary accession number(s): P77693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

CysPTWAM system can also transport molybdate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.