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P16676

- CYSA_ECOLI

UniProt

P16676 - CYSA_ECOLI

Protein

Sulfate/thiosulfate import ATP-binding protein CysA

Gene

cysA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Part of the ABC transporter complex CysAWTP involved in sulfate/thiosulfate import. Responsible for energy coupling to the transport system.

    Catalytic activityi

    ATP + H2O + sulfate(Out) = ADP + phosphate + sulfate(In).UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi35 – 428ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfate transmembrane-transporting ATPase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sulfate transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:CYSA-MONOMER.
    ECOL316407:JW2415-MONOMER.
    MetaCyc:CYSA-MONOMER.

    Protein family/group databases

    TCDBi3.A.1.6.1. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfate/thiosulfate import ATP-binding protein CysAUniRule annotation (EC:3.6.3.25UniRule annotation)
    Alternative name(s):
    Sulfate-transporting ATPaseUniRule annotation
    Gene namesi
    Name:cysAUniRule annotation
    Ordered Locus Names:b2422, JW2415
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10183. cysA.

    Subcellular locationi

    Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

    GO - Cellular componenti

    1. ATP-binding cassette (ABC) transporter complex Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365Sulfate/thiosulfate import ATP-binding protein CysAPRO_0000092265Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP16676.

    Interactioni

    Subunit structurei

    The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-9373N.
    IntActiP16676. 4 interactions.
    MINTiMINT-1267862.
    STRINGi511145.b2422.

    Structurei

    3D structure databases

    ProteinModelPortaliP16676.
    SMRiP16676. Positions 1-347.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 237235ABC transporterUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family. [View classification]UniRule annotation
    Contains 1 ABC transporter domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1118.
    KOiK02045.
    OMAiDEMHITS.
    OrthoDBiEOG6T7N3V.
    PhylomeDBiP16676.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR014769. ABC_CysA_ATP-bd_C.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR008995. Mo/tungstate-bd_C_term_dom.
    IPR027417. P-loop_NTPase.
    IPR005666. Sulph_transpt1.
    IPR024765. TOBE-like.
    [Graphical view]
    PfamiPF00005. ABC_tran. 1 hit.
    PF12857. TOBE_3. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50331. SSF50331. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00968. 3a0106s01. 1 hit.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51237. CYSA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16676-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIEIANIKK SFGRTQVLND ISLDIPSGQM VALLGPSGSG KTTLLRIIAG    50
    LEHQTSGHIR FHGTDVSRLH ARDRKVGFVF QHYALFRHMT VFDNIAFGLT 100
    VLPRRERPNA AAIKAKVTKL LEMVQLAHLA DRYPAQLSGG QKQRVALARA 150
    LAVEPQILLL DEPFGALDAQ VRKELRRWLR QLHEELKFTS VFVTHDQEEA 200
    TEVADRVVVM SQGNIEQADA PDQVWREPAT RFVLEFMGEV NRLQGTIRGG 250
    QFHVGAHRWP LGYTPAYQGP VDLFLRPWEV DISRRTSLDS PLPVQVLEAS 300
    PKGHYTQLVV QPLGWYNEPL TVVMHGDDAP QRGERLFVGL QHARLYNGDE 350
    RIETRDEELA LAQSA 365
    Length:365
    Mass (Da):41,059
    Last modified:November 1, 1997 - v2
    Checksum:iB5FCCA346EDF2788
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti136 – 1372QL → HV in AAA23639. (PubMed:2188958)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32101 Genomic DNA. Translation: AAA23639.1.
    U00096 Genomic DNA. Translation: AAC75475.1.
    AP009048 Genomic DNA. Translation: BAA16296.1.
    PIRiE65016. QRECSA.
    RefSeqiNP_416917.1. NC_000913.3.
    YP_490658.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75475; AAC75475; b2422.
    BAA16296; BAA16296; BAA16296.
    GeneIDi12931761.
    946889.
    KEGGiecj:Y75_p2383.
    eco:b2422.
    PATRICi32120229. VBIEscCol129921_2517.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32101 Genomic DNA. Translation: AAA23639.1 .
    U00096 Genomic DNA. Translation: AAC75475.1 .
    AP009048 Genomic DNA. Translation: BAA16296.1 .
    PIRi E65016. QRECSA.
    RefSeqi NP_416917.1. NC_000913.3.
    YP_490658.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P16676.
    SMRi P16676. Positions 1-347.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9373N.
    IntActi P16676. 4 interactions.
    MINTi MINT-1267862.
    STRINGi 511145.b2422.

    Protein family/group databases

    TCDBi 3.A.1.6.1. the atp-binding cassette (abc) superfamily.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75475 ; AAC75475 ; b2422 .
    BAA16296 ; BAA16296 ; BAA16296 .
    GeneIDi 12931761.
    946889.
    KEGGi ecj:Y75_p2383.
    eco:b2422.
    PATRICi 32120229. VBIEscCol129921_2517.

    Organism-specific databases

    EchoBASEi EB0180.
    EcoGenei EG10183. cysA.

    Phylogenomic databases

    eggNOGi COG1118.
    KOi K02045.
    OMAi DEMHITS.
    OrthoDBi EOG6T7N3V.
    PhylomeDBi P16676.

    Enzyme and pathway databases

    BioCyci EcoCyc:CYSA-MONOMER.
    ECOL316407:JW2415-MONOMER.
    MetaCyc:CYSA-MONOMER.

    Miscellaneous databases

    PROi P16676.

    Gene expression databases

    Genevestigatori P16676.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR014769. ABC_CysA_ATP-bd_C.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR008995. Mo/tungstate-bd_C_term_dom.
    IPR027417. P-loop_NTPase.
    IPR005666. Sulph_transpt1.
    IPR024765. TOBE-like.
    [Graphical view ]
    Pfami PF00005. ABC_tran. 1 hit.
    PF12857. TOBE_3. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50331. SSF50331. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00968. 3a0106s01. 1 hit.
    PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51237. CYSA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster."
      Sirko A., Hryniewicz M.M., Hulanicka D.M., Boeck A.
      J. Bacteriol. 172:3351-3357(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli."
      Rosentel J.K., Healy F., Maupin-Furlow J.A., Lee J.H., Shanmugam K.T.
      J. Bacteriol. 177:4857-4864(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE MOLYBDATE TRANSPORT.

    Entry informationi

    Entry nameiCYSA_ECOLI
    AccessioniPrimary (citable) accession number: P16676
    Secondary accession number(s): P77693
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    CysPTWAM system can also transport molybdate.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3