P16676 (CYSA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate/thiosulfate import ATP-binding protein CysA
EC=3.6.3.25

Alternative name(s):
Sulfate-transporting ATPase

Gene names

Name:	cysA
Ordered Locus Names:	b2422, JW2415

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex CysAWTP involved in sulfate/thiosulfate import. Responsible for energy coupling to the transport system.
Catalytic activity	ATP + H2O + sulfate(Out) = ADP + phosphate + sulfate(In).
Subunit structure	The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Miscellaneous	CysPTWAM system can also transport molybdate.
Sequence similarities	Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Sulfate transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC sulfate transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate transmembrane-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Sulfate/thiosulfate import ATP-binding protein CysA		PRO_0000092265
Regions
	Domain	3 – 237	235	ABC transporter
	Nucleotide binding	35 – 42	8	ATP Potential
Experimental info
	Sequence conflict	136 – 137	2	QL → HV in AAA23639. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P16676 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: B5FCCA346EDF2788	FASTA	365	41,059
	10 20 30 40 50 60 MSIEIANIKK SFGRTQVLND ISLDIPSGQM VALLGPSGSG KTTLLRIIAG LEHQTSGHIR 70 80 90 100 110 120 FHGTDVSRLH ARDRKVGFVF QHYALFRHMT VFDNIAFGLT VLPRRERPNA AAIKAKVTKL 130 140 150 160 170 180 LEMVQLAHLA DRYPAQLSGG QKQRVALARA LAVEPQILLL DEPFGALDAQ VRKELRRWLR 190 200 210 220 230 240 QLHEELKFTS VFVTHDQEEA TEVADRVVVM SQGNIEQADA PDQVWREPAT RFVLEFMGEV 250 260 270 280 290 300 NRLQGTIRGG QFHVGAHRWP LGYTPAYQGP VDLFLRPWEV DISRRTSLDS PLPVQVLEAS 310 320 330 340 350 360 PKGHYTQLVV QPLGWYNEPL TVVMHGDDAP QRGERLFVGL QHARLYNGDE RIETRDEELA LAQSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide sequence and expression of the cysTWAM gene cluster." Sirko A., Hryniewicz M.M., Hulanicka D.M., Boeck A. J. Bacteriol. 172:3351-3357(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli." Rosentel J.K., Healy F., Maupin-Furlow J.A., Lee J.H., Shanmugam K.T. J. Bacteriol. 177:4857-4864(1995) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE MOLYBDATE TRANSPORT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32101 Genomic DNA. Translation: AAA23639.1. U00096 Genomic DNA. Translation: AAC75475.1. AP009048 Genomic DNA. Translation: BAA16296.1.
PIR	QRECSA. E65016.
RefSeq	NP_416917.1. NC_000913.2. YP_490658.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P16676.
SMR	P16676. Positions 1-347.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9373N.
IntAct	P16676. 4 interactions.
MINT	MINT-1267862.
STRING	511145.b2422.
Protein family/group databases
TCDB	3.A.1.6.1. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75475; AAC75475; b2422. BAA16296; BAA16296; BAA16296.
GeneID	12931761. 946889.
KEGG	ecj:Y75_p2383. eco:b2422.
PATRIC	32120229. VBIEscCol129921_2517.
Organism-specific databases
EchoBASE	EB0180.
EcoGene	EG10183. cysA.
Phylogenomic databases
eggNOG	COG1118.
KO	K02045.
OMA	PWEVDVS.
ProtClustDB	PRK10851.
Enzyme and pathway databases
BioCyc	EcoCyc:CYSA-MONOMER. ECOL316407:JW2415-MONOMER.
Gene expression databases
Genevestigator	P16676.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR026251. ABC_ATP-bd_CysA. IPR014769. ABC_CysA_ATP-bd_C. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR008995. Mo/tungstate-bd_C_term_dom. IPR005666. Sulph_transpt1. IPR024765. TOBE-like. [Graphical view]
PANTHER	PTHR24220:SF48. PTHR24220:SF48. 1 hit.
Pfam	PF00005. ABC_tran. 1 hit. PF12857. TOBE_3. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50331. MOP_like. 1 hit.
TIGRFAMs	TIGR00968. 3a0106s01. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51237. CYSA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSA_ECOLI

Accession

Primary (citable) accession number: P16676
Secondary accession number(s): P77693

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 133 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program