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P16675

- PPGB_MOUSE

UniProt

P16675 - PPGB_MOUSE

Protein

Lysosomal protective protein

Gene

Ctsa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

    Catalytic activityi

    Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei173 – 1731By similarity
    Active sitei394 – 3941By similarity
    Active sitei451 – 4511By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. serine-type carboxypeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Carboxypeptidase, Hydrolase, Protease

    Enzyme and pathway databases

    ReactomeiREACT_199008. Glycosphingolipid metabolism.
    REACT_202500. Sialic acid metabolism.

    Protein family/group databases

    MEROPSiS10.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysosomal protective protein (EC:3.4.16.5)
    Alternative name(s):
    Carboxypeptidase C
    Carboxypeptidase L
    Cathepsin A
    Protective protein cathepsin A
    Short name:
    PPCA
    Protective protein for beta-galactosidase
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Ctsa
    Synonyms:Ppgb
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:97748. Ctsa.

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Add
    BLAST
    Chaini24 – 474451Lysosomal protective proteinPRO_0000004277Add
    BLAST
    Chaini24 – 320297Lysosomal protective protein 32 kDa chainPRO_0000004278Add
    BLAST
    Chaini321 – 474154Lysosomal protective protein 20 kDa chainPRO_0000004279Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi83 ↔ 356By similarity
    Glycosylationi140 – 1401N-linked (GlcNAc...) (high mannose)1 Publication
    Disulfide bondi235 ↔ 251By similarity
    Disulfide bondi236 ↔ 241By similarity
    Disulfide bondi276 ↔ 325By similarity
    Glycosylationi327 – 3271N-linked (GlcNAc...) (high mannose)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP16675.
    PaxDbiP16675.
    PRIDEiP16675.

    PTM databases

    PhosphoSiteiP16675.

    Expressioni

    Gene expression databases

    ArrayExpressiP16675.
    BgeeiP16675.
    CleanExiMM_CTSA.
    GenevestigatoriP16675.

    Interactioni

    Subunit structurei

    Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.By similarity

    Protein-protein interaction databases

    BioGridi202325. 1 interaction.
    IntActiP16675. 2 interactions.
    MINTiMINT-1861341.
    STRINGi10090.ENSMUSP00000104946.

    Structurei

    3D structure databases

    ProteinModelPortaliP16675.
    SMRiP16675. Positions 24-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S10 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2939.
    HOGENOMiHOG000198295.
    HOVERGENiHBG053652.
    InParanoidiP16675.
    KOiK13289.
    OrthoDBiEOG7KQ21H.
    PhylomeDBiP16675.
    TreeFamiTF323769.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view]
    PANTHERiPTHR11802. PTHR11802. 1 hit.
    PfamiPF00450. Peptidase_S10. 1 hit.
    [Graphical view]
    PRINTSiPR00724. CRBOXYPTASEC.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
    PS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P16675-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGTALSPLL LLLLLSWASR NEAAPDQDEI DCLPGLAKQP SFRQYSGYLR    50
    ASDSKHFHYW FVESQNDPKN SPVVLWLNGG PGCSSLDGLL TEHGPFLIQP 100
    DGVTLEYNPY AWNLIANVLY IESPAGVGFS YSDDKMYVTN DTEVAENNYE 150
    ALKDFFRLFP EYKDNKLFLT GESYAGIYIP TLAVLVMQDP SMNLQGLAVG 200
    NGLASYEQND NSLVYFAYYH GLLGNRLWTS LQTHCCAQNK CNFYDNKDPE 250
    CVNNLLEVSR IVGKSGLNIY NLYAPCAGGV PGRHRYEDTL VVQDFGNIFT 300
    RLPLKRRFPE ALMRSGDKVR LDPPCTNTTA PSNYLNNPYV RKALHIPESL 350
    PRWDMCNFLV NLQYRRLYQS MNSQYLKLLS SQKYQILLYN GDVDMACNFM 400
    GDEWFVDSLN QKMEVQRRPW LVDYGESGEQ VAGFVKECSH ITFLTIKGAG 450
    HMVPTDKPRA AFTMFSRFLN KEPY 474
    Length:474
    Mass (Da):53,844
    Last modified:August 1, 1990 - v1
    Checksum:i8F4D5A7F4FEBC6CE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti425 – 4251G → W in AAH18534. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05261 mRNA. Translation: AAA39982.1.
    BC018534 mRNA. Translation: AAH18534.1.
    CCDSiCCDS17062.1.
    PIRiA35732.
    RefSeqiNP_001033581.1. NM_001038492.2.
    UniGeneiMm.359633.
    Mm.474586.

    Genome annotation databases

    EnsembliENSMUST00000103092; ENSMUSP00000099381; ENSMUSG00000017760.
    ENSMUST00000103093; ENSMUSP00000099382; ENSMUSG00000017760.
    GeneIDi19025.
    KEGGimmu:19025.
    UCSCiuc008nwm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05261 mRNA. Translation: AAA39982.1 .
    BC018534 mRNA. Translation: AAH18534.1 .
    CCDSi CCDS17062.1.
    PIRi A35732.
    RefSeqi NP_001033581.1. NM_001038492.2.
    UniGenei Mm.359633.
    Mm.474586.

    3D structure databases

    ProteinModelPortali P16675.
    SMRi P16675. Positions 24-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202325. 1 interaction.
    IntActi P16675. 2 interactions.
    MINTi MINT-1861341.
    STRINGi 10090.ENSMUSP00000104946.

    Protein family/group databases

    MEROPSi S10.002.

    PTM databases

    PhosphoSitei P16675.

    Proteomic databases

    MaxQBi P16675.
    PaxDbi P16675.
    PRIDEi P16675.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103092 ; ENSMUSP00000099381 ; ENSMUSG00000017760 .
    ENSMUST00000103093 ; ENSMUSP00000099382 ; ENSMUSG00000017760 .
    GeneIDi 19025.
    KEGGi mmu:19025.
    UCSCi uc008nwm.1. mouse.

    Organism-specific databases

    CTDi 5476.
    MGIi MGI:97748. Ctsa.

    Phylogenomic databases

    eggNOGi COG2939.
    HOGENOMi HOG000198295.
    HOVERGENi HBG053652.
    InParanoidi P16675.
    KOi K13289.
    OrthoDBi EOG7KQ21H.
    PhylomeDBi P16675.
    TreeFami TF323769.

    Enzyme and pathway databases

    Reactomei REACT_199008. Glycosphingolipid metabolism.
    REACT_202500. Sialic acid metabolism.

    Miscellaneous databases

    ChiTaRSi CTSA. mouse.
    NextBioi 295464.
    PROi P16675.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P16675.
    Bgeei P16675.
    CleanExi MM_CTSA.
    Genevestigatori P16675.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR001563. Peptidase_S10.
    IPR018202. Peptidase_S10_AS.
    [Graphical view ]
    PANTHERi PTHR11802. PTHR11802. 1 hit.
    Pfami PF00450. Peptidase_S10. 1 hit.
    [Graphical view ]
    PRINTSi PR00724. CRBOXYPTASEC.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00560. CARBOXYPEPT_SER_HIS. 1 hit.
    PS00131. CARBOXYPEPT_SER_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse 'protective protein'. cDNA cloning, sequence comparison, and expression."
      Galjart N.J., Gillemans N., Meijer D., D'Azzo A.
      J. Biol. Chem. 265:4678-4684(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis."
      Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S.
      Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140 AND ASN-327.
      Tissue: Epidermis.

    Entry informationi

    Entry nameiPPGB_MOUSE
    AccessioniPrimary (citable) accession number: P16675
    Secondary accession number(s): Q8VEF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3