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Protein

Lysosomal protective protein

Gene

Ctsa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173By similarity1
Active sitei394By similarity1
Active sitei451By similarity1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
  • proteolysis Source: ParkinsonsUK-UCL
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
  • regulation of protein stability Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Enzyme and pathway databases

BRENDAi3.4.16.5. 3474.
ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-4085001. Sialic acid metabolism.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

ESTHERimouse-Ppgb. Carboxypeptidase_S10.
MEROPSiS10.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal protective protein (EC:3.4.16.5)
Alternative name(s):
Carboxypeptidase C
Carboxypeptidase L
Cathepsin A
Protective protein cathepsin A
Short name:
PPCA
Protective protein for beta-galactosidase
Cleaved into the following 2 chains:
Gene namesi
Name:Ctsa
Synonyms:Ppgb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:97748. Ctsa.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3259490.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000000427724 – 474Lysosomal protective proteinAdd BLAST451
ChainiPRO_000000427824 – 320Lysosomal protective protein 32 kDa chainAdd BLAST297
ChainiPRO_0000004279321 – 474Lysosomal protective protein 20 kDa chainAdd BLAST154

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi83 ↔ 356By similarity
Glycosylationi140N-linked (GlcNAc...) (high mannose)1 Publication1
Disulfide bondi235 ↔ 251By similarity
Disulfide bondi236 ↔ 241By similarity
Disulfide bondi276 ↔ 325By similarity
Glycosylationi327N-linked (GlcNAc...) (high mannose)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP16675.
PaxDbiP16675.
PeptideAtlasiP16675.
PRIDEiP16675.

PTM databases

PhosphoSitePlusiP16675.
UniCarbKBiP16675.

Expressioni

Gene expression databases

BgeeiENSMUSG00000017760.
CleanExiMM_CTSA.
ExpressionAtlasiP16675. baseline and differential.
GenevisibleiP16675. MM.

Interactioni

Subunit structurei

Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.By similarity

Protein-protein interaction databases

BioGridi202325. 1 interactor.
IntActiP16675. 2 interactors.
MINTiMINT-1861341.
STRINGi10090.ENSMUSP00000017904.

Structurei

3D structure databases

ProteinModelPortaliP16675.
SMRiP16675.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.
GeneTreeiENSGT00860000133774.
HOGENOMiHOG000198295.
HOVERGENiHBG053652.
InParanoidiP16675.
KOiK13289.
PhylomeDBiP16675.
TreeFamiTF323769.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 2 hits.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16675-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGTALSPLL LLLLLSWASR NEAAPDQDEI DCLPGLAKQP SFRQYSGYLR
60 70 80 90 100
ASDSKHFHYW FVESQNDPKN SPVVLWLNGG PGCSSLDGLL TEHGPFLIQP
110 120 130 140 150
DGVTLEYNPY AWNLIANVLY IESPAGVGFS YSDDKMYVTN DTEVAENNYE
160 170 180 190 200
ALKDFFRLFP EYKDNKLFLT GESYAGIYIP TLAVLVMQDP SMNLQGLAVG
210 220 230 240 250
NGLASYEQND NSLVYFAYYH GLLGNRLWTS LQTHCCAQNK CNFYDNKDPE
260 270 280 290 300
CVNNLLEVSR IVGKSGLNIY NLYAPCAGGV PGRHRYEDTL VVQDFGNIFT
310 320 330 340 350
RLPLKRRFPE ALMRSGDKVR LDPPCTNTTA PSNYLNNPYV RKALHIPESL
360 370 380 390 400
PRWDMCNFLV NLQYRRLYQS MNSQYLKLLS SQKYQILLYN GDVDMACNFM
410 420 430 440 450
GDEWFVDSLN QKMEVQRRPW LVDYGESGEQ VAGFVKECSH ITFLTIKGAG
460 470
HMVPTDKPRA AFTMFSRFLN KEPY
Length:474
Mass (Da):53,844
Last modified:August 1, 1990 - v1
Checksum:i8F4D5A7F4FEBC6CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti425G → W in AAH18534 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05261 mRNA. Translation: AAA39982.1.
BC018534 mRNA. Translation: AAH18534.1.
CCDSiCCDS17062.1.
PIRiA35732.
RefSeqiNP_001033581.1. NM_001038492.2.
UniGeneiMm.359633.
Mm.474586.

Genome annotation databases

EnsembliENSMUST00000103092; ENSMUSP00000099381; ENSMUSG00000017760.
ENSMUST00000103093; ENSMUSP00000099382; ENSMUSG00000017760.
GeneIDi19025.
KEGGimmu:19025.
UCSCiuc008nwm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05261 mRNA. Translation: AAA39982.1.
BC018534 mRNA. Translation: AAH18534.1.
CCDSiCCDS17062.1.
PIRiA35732.
RefSeqiNP_001033581.1. NM_001038492.2.
UniGeneiMm.359633.
Mm.474586.

3D structure databases

ProteinModelPortaliP16675.
SMRiP16675.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202325. 1 interactor.
IntActiP16675. 2 interactors.
MINTiMINT-1861341.
STRINGi10090.ENSMUSP00000017904.

Chemistry databases

ChEMBLiCHEMBL3259490.

Protein family/group databases

ESTHERimouse-Ppgb. Carboxypeptidase_S10.
MEROPSiS10.002.

PTM databases

PhosphoSitePlusiP16675.
UniCarbKBiP16675.

Proteomic databases

EPDiP16675.
PaxDbiP16675.
PeptideAtlasiP16675.
PRIDEiP16675.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103092; ENSMUSP00000099381; ENSMUSG00000017760.
ENSMUST00000103093; ENSMUSP00000099382; ENSMUSG00000017760.
GeneIDi19025.
KEGGimmu:19025.
UCSCiuc008nwm.2. mouse.

Organism-specific databases

CTDi5476.
MGIiMGI:97748. Ctsa.

Phylogenomic databases

eggNOGiKOG1282. Eukaryota.
COG2939. LUCA.
GeneTreeiENSGT00860000133774.
HOGENOMiHOG000198295.
HOVERGENiHBG053652.
InParanoidiP16675.
KOiK13289.
PhylomeDBiP16675.
TreeFamiTF323769.

Enzyme and pathway databases

BRENDAi3.4.16.5. 3474.
ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-4085001. Sialic acid metabolism.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiCtsa. mouse.
PROiP16675.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017760.
CleanExiMM_CTSA.
ExpressionAtlasiP16675. baseline and differential.
GenevisibleiP16675. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 2 hits.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPGB_MOUSE
AccessioniPrimary (citable) accession number: P16675
Secondary accession number(s): Q8VEF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.