Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P16675 (PPGB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysosomal protective protein

EC=3.4.16.5
Alternative name(s):
Carboxypeptidase C
Carboxypeptidase L
Cathepsin A
Protective protein cathepsin A
Short name=PPCA
Protective protein for beta-galactosidase
Gene names
Name:Ctsa
Synonyms:Ppgb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

Catalytic activity

Release of a C-terminal amino acid with broad specificity.

Subunit structure

Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked By similarity.

Subcellular location

Lysosome.

Sequence similarities

Belongs to the peptidase S10 family.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   Molecular functionCarboxypeptidase
Hydrolase
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 14651853. Source: MGI

   Molecular_functionserine-type carboxypeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323
Chain24 – 474451Lysosomal protective protein
PRO_0000004277
Chain24 – 320297Lysosomal protective protein 32 kDa chain
PRO_0000004278
Chain321 – 474154Lysosomal protective protein 20 kDa chain
PRO_0000004279

Sites

Active site1731 By similarity
Active site3941 By similarity
Active site4511 By similarity

Amino acid modifications

Glycosylation1401N-linked (GlcNAc...) (high mannose) Ref.3
Glycosylation3271N-linked (GlcNAc...) (high mannose) Ref.3
Disulfide bond83 ↔ 356 By similarity
Disulfide bond235 ↔ 251 By similarity
Disulfide bond236 ↔ 241 By similarity
Disulfide bond276 ↔ 325 By similarity

Experimental info

Sequence conflict4251G → W in AAH18534. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P16675 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 8F4D5A7F4FEBC6CE

FASTA47453,844
        10         20         30         40         50         60 
MPGTALSPLL LLLLLSWASR NEAAPDQDEI DCLPGLAKQP SFRQYSGYLR ASDSKHFHYW 

        70         80         90        100        110        120 
FVESQNDPKN SPVVLWLNGG PGCSSLDGLL TEHGPFLIQP DGVTLEYNPY AWNLIANVLY 

       130        140        150        160        170        180 
IESPAGVGFS YSDDKMYVTN DTEVAENNYE ALKDFFRLFP EYKDNKLFLT GESYAGIYIP 

       190        200        210        220        230        240 
TLAVLVMQDP SMNLQGLAVG NGLASYEQND NSLVYFAYYH GLLGNRLWTS LQTHCCAQNK 

       250        260        270        280        290        300 
CNFYDNKDPE CVNNLLEVSR IVGKSGLNIY NLYAPCAGGV PGRHRYEDTL VVQDFGNIFT 

       310        320        330        340        350        360 
RLPLKRRFPE ALMRSGDKVR LDPPCTNTTA PSNYLNNPYV RKALHIPESL PRWDMCNFLV 

       370        380        390        400        410        420 
NLQYRRLYQS MNSQYLKLLS SQKYQILLYN GDVDMACNFM GDEWFVDSLN QKMEVQRRPW 

       430        440        450        460        470 
LVDYGESGEQ VAGFVKECSH ITFLTIKGAG HMVPTDKPRA AFTMFSRFLN KEPY 

« Hide

References

« Hide 'large scale' references
[1]"Mouse 'protective protein'. cDNA cloning, sequence comparison, and expression."
Galjart N.J., Gillemans N., Meijer D., D'Azzo A.
J. Biol. Chem. 265:4678-4684(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis."
Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S.
Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140 AND ASN-327.
Tissue: Epidermis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05261 mRNA. Translation: AAA39982.1.
BC018534 mRNA. Translation: AAH18534.1.
PIRA35732.
RefSeqNP_001033581.1. NM_001038492.2.
UniGeneMm.359633.
Mm.474586.

3D structure databases

ProteinModelPortalP16675.
SMRP16675. Positions 24-474.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202325. 1 interaction.
IntActP16675. 2 interactions.
MINTMINT-1861341.
STRING10090.ENSMUSP00000104946.

Protein family/group databases

MEROPSS10.002.

PTM databases

PhosphoSiteP16675.

Proteomic databases

PaxDbP16675.
PRIDEP16675.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103092; ENSMUSP00000099381; ENSMUSG00000017760.
ENSMUST00000103093; ENSMUSP00000099382; ENSMUSG00000017760.
GeneID19025.
KEGGmmu:19025.
UCSCuc008nwm.1. mouse.

Organism-specific databases

CTD5476.
MGIMGI:97748. Ctsa.

Phylogenomic databases

eggNOGCOG2939.
HOGENOMHOG000198295.
HOVERGENHBG053652.
InParanoidP16675.
KOK13289.
OrthoDBEOG7KQ21H.
PhylomeDBP16675.
TreeFamTF323769.

Gene expression databases

ArrayExpressP16675.
BgeeP16675.
CleanExMM_CTSA.
GenevestigatorP16675.

Family and domain databases

InterProIPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERPTHR11802. PTHR11802. 1 hit.
PfamPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSPR00724. CRBOXYPTASEC.
PROSITEPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCTSA. mouse.
NextBio295464.
PROP16675.
SOURCESearch...

Entry information

Entry namePPGB_MOUSE
AccessionPrimary (citable) accession number: P16675
Secondary accession number(s): Q8VEF6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot