P16675 (PPGB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysosomal protective protein
EC=3.4.16.5

Alternative name(s):
Carboxypeptidase C
Carboxypeptidase L
Cathepsin A
Protective protein cathepsin A
Short name=PPCA
Protective protein for beta-galactosidase

Cleaved into the following 2 chains:

1. Lysosomal protective protein 32 kDa chain
2. Lysosomal protective protein 20 kDa chain

Gene names

Name:	Ctsa
Synonyms:	Ppgb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
Catalytic activity	Release of a C-terminal amino acid with broad specificity.
Subunit structure	Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked By similarity.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the peptidase S10 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Carboxypeptidase Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 14651853. Source: MGI
Molecular_function	serine-type carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 474	451	Lysosomal protective protein		PRO_0000004277
	Chain	24 – 320	297	Lysosomal protective protein 32 kDa chain		PRO_0000004278
	Chain	321 – 474	154	Lysosomal protective protein 20 kDa chain		PRO_0000004279
Sites
	Active site	173	1	By similarity
	Active site	394	1	By similarity
	Active site	451	1	By similarity
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) (high mannose) Ref.3
	Glycosylation	327	1	N-linked (GlcNAc...) (high mannose) Ref.3
	Disulfide bond	83 ↔ 356		By similarity
	Disulfide bond	235 ↔ 251		By similarity
	Disulfide bond	236 ↔ 241		By similarity
	Disulfide bond	276 ↔ 325		By similarity
Experimental info
	Sequence conflict	425	1	G → W in AAH18534. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P16675 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: 8F4D5A7F4FEBC6CE	FASTA	474	53,844
	10 20 30 40 50 60 MPGTALSPLL LLLLLSWASR NEAAPDQDEI DCLPGLAKQP SFRQYSGYLR ASDSKHFHYW 70 80 90 100 110 120 FVESQNDPKN SPVVLWLNGG PGCSSLDGLL TEHGPFLIQP DGVTLEYNPY AWNLIANVLY 130 140 150 160 170 180 IESPAGVGFS YSDDKMYVTN DTEVAENNYE ALKDFFRLFP EYKDNKLFLT GESYAGIYIP 190 200 210 220 230 240 TLAVLVMQDP SMNLQGLAVG NGLASYEQND NSLVYFAYYH GLLGNRLWTS LQTHCCAQNK 250 260 270 280 290 300 CNFYDNKDPE CVNNLLEVSR IVGKSGLNIY NLYAPCAGGV PGRHRYEDTL VVQDFGNIFT 310 320 330 340 350 360 RLPLKRRFPE ALMRSGDKVR LDPPCTNTTA PSNYLNNPYV RKALHIPESL PRWDMCNFLV 370 380 390 400 410 420 NLQYRRLYQS MNSQYLKLLS SQKYQILLYN GDVDMACNFM GDEWFVDSLN QKMEVQRRPW 430 440 450 460 470 LVDYGESGEQ VAGFVKECSH ITFLTIKGAG HMVPTDKPRA AFTMFSRFLN KEPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse 'protective protein'. cDNA cloning, sequence comparison, and expression." Galjart N.J., Gillemans N., Meijer D., D'Azzo A. J. Biol. Chem. 265:4678-4684(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis." Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S. Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140 AND ASN-327, MASS SPECTROMETRY. Tissue: Epidermis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05261 mRNA. Translation: AAA39982.1. BC018534 mRNA. Translation: AAH18534.1.
IPI	IPI00137177.
PIR	A35732.
RefSeq	NP_001033581.1. NM_001038492.2.
UniGene	Mm.359633. Mm.474586.
3D structure databases
ProteinModelPortal	P16675.
SMR	P16675. Positions 24-474.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000104946.
Protein family/group databases
MEROPS	S10.002.
PTM databases
PhosphoSite	P16675.
Proteomic databases
PaxDb	P16675.
PRIDE	P16675.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000103092; ENSMUSP00000099381; ENSMUSG00000017760. ENSMUST00000103093; ENSMUSP00000099382; ENSMUSG00000017760.
GeneID	19025.
KEGG	mmu:19025.
Organism-specific databases
CTD	5476.
MGI	MGI:97748. Ctsa.
Phylogenomic databases
eggNOG	COG2939.
HOGENOM	HOG000198295.
HOVERGEN	HBG053652.
InParanoid	P16675.
KO	K13289.
OrthoDB	EOG4Z0B5K.
Gene expression databases
ArrayExpress	P16675.
Bgee	P16675.
CleanEx	MM_CTSA.
Genevestigator	P16675.
GermOnline	ENSMUSG00000017760. Mus musculus.
Family and domain databases
InterPro	IPR001563. Peptidase_S10. IPR018202. Peptidase_S10_AS. [Graphical view]
PANTHER	PTHR11802. PTHR11802. 1 hit.
Pfam	PF00450. Peptidase_S10. 1 hit. [Graphical view]
PRINTS	PR00724. CRBOXYPTASEC.
PROSITE	PS00560. CARBOXYPEPT_SER_HIS. 1 hit. PS00131. CARBOXYPEPT_SER_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CTSA. mouse.
NextBio	295464.
SOURCE	Search...

Entry information

Entry name

PPGB_MOUSE

Accession

Primary (citable) accession number: P16675
Secondary accession number(s): Q8VEF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program