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Protein

Lysosomal protective protein

Gene

Ctsa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

Catalytic activityi

Release of a C-terminal amino acid with broad specificity.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731By similarity
Active sitei394 – 3941By similarity
Active sitei451 – 4511By similarity

GO - Molecular functioni

  1. serine-type carboxypeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiREACT_199008. Glycosphingolipid metabolism.
REACT_202500. Sialic acid metabolism.

Protein family/group databases

MEROPSiS10.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal protective protein (EC:3.4.16.5)
Alternative name(s):
Carboxypeptidase C
Carboxypeptidase L
Cathepsin A
Protective protein cathepsin A
Short name:
PPCA
Protective protein for beta-galactosidase
Cleaved into the following 2 chains:
Gene namesi
Name:Ctsa
Synonyms:Ppgb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:97748. Ctsa.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. intracellular membrane-bounded organelle Source: MGI
  3. lysosome Source: UniProtKB-SubCell
  4. membrane Source: MGI
  5. mitochondrion Source: MGI
  6. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Add
BLAST
Chaini24 – 474451Lysosomal protective proteinPRO_0000004277Add
BLAST
Chaini24 – 320297Lysosomal protective protein 32 kDa chainPRO_0000004278Add
BLAST
Chaini321 – 474154Lysosomal protective protein 20 kDa chainPRO_0000004279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi83 ↔ 356By similarity
Glycosylationi140 – 1401N-linked (GlcNAc...) (high mannose)1 Publication
Disulfide bondi235 ↔ 251By similarity
Disulfide bondi236 ↔ 241By similarity
Disulfide bondi276 ↔ 325By similarity
Glycosylationi327 – 3271N-linked (GlcNAc...) (high mannose)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP16675.
PaxDbiP16675.
PRIDEiP16675.

PTM databases

PhosphoSiteiP16675.

Expressioni

Gene expression databases

BgeeiP16675.
CleanExiMM_CTSA.
ExpressionAtlasiP16675. baseline and differential.
GenevestigatoriP16675.

Interactioni

Subunit structurei

Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.By similarity

Protein-protein interaction databases

BioGridi202325. 1 interaction.
IntActiP16675. 2 interactions.
MINTiMINT-1861341.
STRINGi10090.ENSMUSP00000104946.

Structurei

3D structure databases

ProteinModelPortaliP16675.
SMRiP16675. Positions 24-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2939.
HOGENOMiHOG000198295.
HOVERGENiHBG053652.
InParanoidiP16675.
KOiK13289.
OrthoDBiEOG7KQ21H.
PhylomeDBiP16675.
TreeFamiTF323769.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P16675-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGTALSPLL LLLLLSWASR NEAAPDQDEI DCLPGLAKQP SFRQYSGYLR
60 70 80 90 100
ASDSKHFHYW FVESQNDPKN SPVVLWLNGG PGCSSLDGLL TEHGPFLIQP
110 120 130 140 150
DGVTLEYNPY AWNLIANVLY IESPAGVGFS YSDDKMYVTN DTEVAENNYE
160 170 180 190 200
ALKDFFRLFP EYKDNKLFLT GESYAGIYIP TLAVLVMQDP SMNLQGLAVG
210 220 230 240 250
NGLASYEQND NSLVYFAYYH GLLGNRLWTS LQTHCCAQNK CNFYDNKDPE
260 270 280 290 300
CVNNLLEVSR IVGKSGLNIY NLYAPCAGGV PGRHRYEDTL VVQDFGNIFT
310 320 330 340 350
RLPLKRRFPE ALMRSGDKVR LDPPCTNTTA PSNYLNNPYV RKALHIPESL
360 370 380 390 400
PRWDMCNFLV NLQYRRLYQS MNSQYLKLLS SQKYQILLYN GDVDMACNFM
410 420 430 440 450
GDEWFVDSLN QKMEVQRRPW LVDYGESGEQ VAGFVKECSH ITFLTIKGAG
460 470
HMVPTDKPRA AFTMFSRFLN KEPY
Length:474
Mass (Da):53,844
Last modified:August 1, 1990 - v1
Checksum:i8F4D5A7F4FEBC6CE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti425 – 4251G → W in AAH18534. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05261 mRNA. Translation: AAA39982.1.
BC018534 mRNA. Translation: AAH18534.1.
CCDSiCCDS17062.1.
PIRiA35732.
RefSeqiNP_001033581.1. NM_001038492.2.
UniGeneiMm.359633.
Mm.474586.

Genome annotation databases

EnsembliENSMUST00000103092; ENSMUSP00000099381; ENSMUSG00000017760.
ENSMUST00000103093; ENSMUSP00000099382; ENSMUSG00000017760.
GeneIDi19025.
KEGGimmu:19025.
UCSCiuc008nwm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05261 mRNA. Translation: AAA39982.1.
BC018534 mRNA. Translation: AAH18534.1.
CCDSiCCDS17062.1.
PIRiA35732.
RefSeqiNP_001033581.1. NM_001038492.2.
UniGeneiMm.359633.
Mm.474586.

3D structure databases

ProteinModelPortaliP16675.
SMRiP16675. Positions 24-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202325. 1 interaction.
IntActiP16675. 2 interactions.
MINTiMINT-1861341.
STRINGi10090.ENSMUSP00000104946.

Protein family/group databases

MEROPSiS10.002.

PTM databases

PhosphoSiteiP16675.

Proteomic databases

MaxQBiP16675.
PaxDbiP16675.
PRIDEiP16675.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103092; ENSMUSP00000099381; ENSMUSG00000017760.
ENSMUST00000103093; ENSMUSP00000099382; ENSMUSG00000017760.
GeneIDi19025.
KEGGimmu:19025.
UCSCiuc008nwm.1. mouse.

Organism-specific databases

CTDi5476.
MGIiMGI:97748. Ctsa.

Phylogenomic databases

eggNOGiCOG2939.
HOGENOMiHOG000198295.
HOVERGENiHBG053652.
InParanoidiP16675.
KOiK13289.
OrthoDBiEOG7KQ21H.
PhylomeDBiP16675.
TreeFamiTF323769.

Enzyme and pathway databases

ReactomeiREACT_199008. Glycosphingolipid metabolism.
REACT_202500. Sialic acid metabolism.

Miscellaneous databases

ChiTaRSiCtsa. mouse.
NextBioi295464.
PROiP16675.
SOURCEiSearch...

Gene expression databases

BgeeiP16675.
CleanExiMM_CTSA.
ExpressionAtlasiP16675. baseline and differential.
GenevestigatoriP16675.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR018202. Peptidase_S10_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse 'protective protein'. cDNA cloning, sequence comparison, and expression."
    Galjart N.J., Gillemans N., Meijer D., D'Azzo A.
    J. Biol. Chem. 265:4678-4684(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis."
    Uematsu R., Furukawa J., Nakagawa H., Shinohara Y., Deguchi K., Monde K., Nishimura S.
    Mol. Cell. Proteomics 4:1977-1989(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-140 AND ASN-327.
    Tissue: Epidermis.

Entry informationi

Entry nameiPPGB_MOUSE
AccessioniPrimary (citable) accession number: P16675
Secondary accession number(s): Q8VEF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: February 4, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.