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P16668

- MTS3_STAAU

UniProt

P16668 - MTS3_STAAU

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Protein

Modification methylase Sau3AI

Gene

sau3AIM

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on C-4 on both strands and protects the DNA from cleavage by the Sau3AI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3494. M.Sau3AI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase Sau3AI (EC:2.1.1.37)
Short name:
M.Sau3AI
Alternative name(s):
Cytosine-specific methyltransferase Sau3AI
Gene namesi
Name:sau3AIM
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Modification methylase Sau3AIPRO_0000087907Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP16668.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 402399SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16668 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKIKVVELF AGVGGFRLGL ENTKNGIFDI TWANQWEPSR KIQHAFDCYS
60 70 80 90 100
KRFKNGIHSN KDIAQVSDEE MANTEADMIV GGFPCQDYSV ARSLNGELGI
110 120 130 140 150
QGKKGVLFWQ IIRYIQNTFP KYLLLENVDR LLKSPSSQRG RDFAVMLSTL
160 170 180 190 200
NELGYNVEWR VINAADYGNA QRRRRVFIFG YKQDLNYSKA MEESPLDKII
210 220 230 240 250
YHNGLFAEAF PIEDYANKNR VNRTHITHDI VDISDNFSFQ FYNSGIMKNG
260 270 280 290 300
EILTIDTIPK YEKSVTLGEI IESNVDDGFS LNQDQIDKFK YLRGPKKIKR
310 320 330 340 350
TTKDGHEYYF SEGGMSETDS LELPARTMLT SESSINRSTH FLNVDGVYRT
360 370 380 390 400
LTPIEAERLN GFPDNWTEGM PIKMRYFCMG NALVVPLITR IGNQIEKIDS
410
ITNDEFSQLR LF
Length:412
Mass (Da):47,295
Last modified:August 1, 1990 - v1
Checksum:iE6F008248749F085
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32470 Genomic DNA. Translation: AAA26673.1.
PIRiJQ0760.
RefSeqiWP_001033636.1. NZ_JJOP01000003.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32470 Genomic DNA. Translation: AAA26673.1 .
PIRi JQ0760.
RefSeqi WP_001033636.1. NZ_JJOP01000003.1.

3D structure databases

ProteinModelPortali P16668.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3494. M.Sau3AI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression and characterization of the Sau3AI restriction and modification genes in Staphylococcus carnosus TM300."
    Seeber S., Kessler C., Goetz F.
    Gene 94:37-43(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49834 / 3A.

Entry informationi

Entry nameiMTS3_STAAU
AccessioniPrimary (citable) accession number: P16668
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3