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P16668

- MTS3_STAAU

UniProt

P16668 - MTS3_STAAU

Protein

Modification methylase Sau3AI

Gene

sau3AIM

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    • Comment

    Functioni

    This methylase recognizes the double-stranded sequence GATC, causes specific methylation on C-4 on both strands and protects the DNA from cleavage by the Sau3AI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3494. M.Sau3AI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase Sau3AI (EC:2.1.1.37)
    Short name:
    M.Sau3AI
    Alternative name(s):
    Cytosine-specific methyltransferase Sau3AI
    Gene namesi
    Name:sau3AIM
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 412412Modification methylase Sau3AIPRO_0000087907Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP16668.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 402399SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16668-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKIKVVELF AGVGGFRLGL ENTKNGIFDI TWANQWEPSR KIQHAFDCYS    50
    KRFKNGIHSN KDIAQVSDEE MANTEADMIV GGFPCQDYSV ARSLNGELGI 100
    QGKKGVLFWQ IIRYIQNTFP KYLLLENVDR LLKSPSSQRG RDFAVMLSTL 150
    NELGYNVEWR VINAADYGNA QRRRRVFIFG YKQDLNYSKA MEESPLDKII 200
    YHNGLFAEAF PIEDYANKNR VNRTHITHDI VDISDNFSFQ FYNSGIMKNG 250
    EILTIDTIPK YEKSVTLGEI IESNVDDGFS LNQDQIDKFK YLRGPKKIKR 300
    TTKDGHEYYF SEGGMSETDS LELPARTMLT SESSINRSTH FLNVDGVYRT 350
    LTPIEAERLN GFPDNWTEGM PIKMRYFCMG NALVVPLITR IGNQIEKIDS 400
    ITNDEFSQLR LF 412
    Length:412
    Mass (Da):47,295
    Last modified:August 1, 1990 - v1
    Checksum:iE6F008248749F085
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32470 Genomic DNA. Translation: AAA26673.1.
    PIRiJQ0760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32470 Genomic DNA. Translation: AAA26673.1 .
    PIRi JQ0760.

    3D structure databases

    ProteinModelPortali P16668.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3494. M.Sau3AI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression and characterization of the Sau3AI restriction and modification genes in Staphylococcus carnosus TM300."
      Seeber S., Kessler C., Goetz F.
      Gene 94:37-43(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 49834 / 3A.

    Entry informationi

    Entry nameiMTS3_STAAU
    AccessioniPrimary (citable) accession number: P16668
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3