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P16668 (MTS3_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase Sau3AI

Short name=M.Sau3AI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase Sau3AI
Gene names
Name:sau3AIM
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GATC, causes specific methylation on C-4 on both strands and protects the DNA from cleavage by the Sau3AI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Modification methylase Sau3AI
PRO_0000087907

Regions

Domain4 – 402399SAM-dependent MTase C5-type

Sites

Active site851 By similarity

Sequences

Sequence LengthMass (Da)Tools
P16668 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: E6F008248749F085

FASTA41247,295
        10         20         30         40         50         60 
MNKIKVVELF AGVGGFRLGL ENTKNGIFDI TWANQWEPSR KIQHAFDCYS KRFKNGIHSN 

        70         80         90        100        110        120 
KDIAQVSDEE MANTEADMIV GGFPCQDYSV ARSLNGELGI QGKKGVLFWQ IIRYIQNTFP 

       130        140        150        160        170        180 
KYLLLENVDR LLKSPSSQRG RDFAVMLSTL NELGYNVEWR VINAADYGNA QRRRRVFIFG 

       190        200        210        220        230        240 
YKQDLNYSKA MEESPLDKII YHNGLFAEAF PIEDYANKNR VNRTHITHDI VDISDNFSFQ 

       250        260        270        280        290        300 
FYNSGIMKNG EILTIDTIPK YEKSVTLGEI IESNVDDGFS LNQDQIDKFK YLRGPKKIKR 

       310        320        330        340        350        360 
TTKDGHEYYF SEGGMSETDS LELPARTMLT SESSINRSTH FLNVDGVYRT LTPIEAERLN 

       370        380        390        400        410 
GFPDNWTEGM PIKMRYFCMG NALVVPLITR IGNQIEKIDS ITNDEFSQLR LF 

« Hide

References

[1]"Cloning, expression and characterization of the Sau3AI restriction and modification genes in Staphylococcus carnosus TM300."
Seeber S., Kessler C., Goetz F.
Gene 94:37-43(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49834 / 3A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M32470 Genomic DNA. Translation: AAA26673.1.
PIRJQ0760.

3D structure databases

ProteinModelPortalP16668.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3494. M.Sau3AI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTS3_STAAU
AccessionPrimary (citable) accession number: P16668
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries