Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P16667

- T2S3_STAAU

UniProt

P16667 - T2S3_STAAU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Type-2 restriction enzyme Sau3AI

Gene

sau3AIR

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GATC and cleaves before G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi1604. Sau3AI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme Sau3AI (EC:3.1.21.4)
Short name:
R.Sau3AI
Alternative name(s):
Endonuclease Sau3AI
Type II restriction enzyme Sau3AI
Gene namesi
Name:sau3AIR
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Type-2 restriction enzyme Sau3AIPRO_0000077358Add
BLAST

Structurei

Secondary structure

1
489
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi237 – 2459Combined sources
Helixi246 – 2483Combined sources
Helixi253 – 2597Combined sources
Helixi269 – 27810Combined sources
Beta strandi301 – 3088Combined sources
Helixi325 – 3295Combined sources
Helixi343 – 3508Combined sources
Beta strandi352 – 3609Combined sources
Beta strandi366 – 3749Combined sources
Helixi378 – 3825Combined sources
Helixi384 – 39815Combined sources
Beta strandi401 – 4066Combined sources
Beta strandi413 – 4186Combined sources
Helixi423 – 4253Combined sources
Beta strandi427 – 43610Combined sources
Beta strandi440 – 4434Combined sources
Beta strandi446 – 4483Combined sources
Beta strandi454 – 4574Combined sources
Beta strandi466 – 4705Combined sources
Beta strandi472 – 4765Combined sources
Helixi478 – 4858Combined sources
Helixi486 – 4883Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2REUX-ray1.90A233-489[»]
ProteinModelPortaliP16667.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP16667.

Family & Domainsi

Family and domain databases

Gene3Di3.40.600.10. 2 hits.
InterProiIPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamiPF02976. MutH. 1 hit.
[Graphical view]
SMARTiSM00927. MutH. 1 hit.
[Graphical view]
SUPFAMiSSF52980. SSF52980. 2 hits.

Sequencei

Sequence statusi: Complete.

P16667-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESYLTKQAV HNRAKEAVGK SVLELNGGES IKQSKSSVGD AFENWFGKKK
60 70 80 90 100
DSDSKPDMAE AGVELKATPF KKLKNGKYSS KERLVLNIIN YEKVANENFE
110 120 130 140 150
TSSFLSKNNT IELAFYEYIK GTPSDNWIIK EAVLYEMHKN PIDYEIIKQD
160 170 180 190 200
WEIINQYINE GKAHELSEGL TSYLAPCTKG ANASSLRNQP YSDIKAKQRA
210 220 230 240 250
FSLKSGYMTS ILRKYVLGDE KIDSIVKDPF EIKEKSIEDI VFEKFQPYIN
260 270 280 290 300
WSIDKLCEHF SINKGEKGLN YRIASAILNL KGKTTKSKPF PEVEEFEKSS
310 320 330 340 350
IVVKTVHFNK KNVNKESMSF GAFKFEELAN EEWEDSEGYP SAQWRNFLLE
360 370 380 390 400
TRFLFFVVKE DEDGVDIFKG IKFFSMPEED INGPVKRMWD DTVKKLKEGV
410 420 430 440 450
TLEAVPDKST KDGWRIKNNF VDKSDDLICH VRPHTNNRDY RGGSNADKLP
460 470 480
KKINWINRPD SDDYSDEWMT KQSFWINNDY IKKQVEDLL
Length:489
Mass (Da):56,471
Last modified:August 1, 1990 - v1
Checksum:iFFC3762A949B86EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32470 Genomic DNA. Translation: AAA26672.1.
PIRiJQ0759.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32470 Genomic DNA. Translation: AAA26672.1 .
PIRi JQ0759.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2REU X-ray 1.90 A 233-489 [» ]
ProteinModelPortali P16667.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1604. Sau3AI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P16667.

Family and domain databases

Gene3Di 3.40.600.10. 2 hits.
InterProi IPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view ]
Pfami PF02976. MutH. 1 hit.
[Graphical view ]
SMARTi SM00927. MutH. 1 hit.
[Graphical view ]
SUPFAMi SSF52980. SSF52980. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Cloning, expression and characterization of the Sau3AI restriction and modification genes in Staphylococcus carnosus TM300."
    Seeber S., Kessler C., Goetz F.
    Gene 94:37-43(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49834 / 3A.

Entry informationi

Entry nameiT2S3_STAAU
AccessioniPrimary (citable) accession number: P16667
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: November 26, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3