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P16667 (T2S3_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme Sau3AI

Short name=R.Sau3AI
EC=3.1.21.4
Alternative name(s):
Endonuclease Sau3AI
Type II restriction enzyme Sau3AI
Gene names
Name:sau3AIR
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GATC and cleaves before G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Type-2 restriction enzyme Sau3AI
PRO_0000077358

Secondary structure

........................................... 489
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P16667 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: FFC3762A949B86EB

FASTA48956,471
        10         20         30         40         50         60 
MESYLTKQAV HNRAKEAVGK SVLELNGGES IKQSKSSVGD AFENWFGKKK DSDSKPDMAE 

        70         80         90        100        110        120 
AGVELKATPF KKLKNGKYSS KERLVLNIIN YEKVANENFE TSSFLSKNNT IELAFYEYIK 

       130        140        150        160        170        180 
GTPSDNWIIK EAVLYEMHKN PIDYEIIKQD WEIINQYINE GKAHELSEGL TSYLAPCTKG 

       190        200        210        220        230        240 
ANASSLRNQP YSDIKAKQRA FSLKSGYMTS ILRKYVLGDE KIDSIVKDPF EIKEKSIEDI 

       250        260        270        280        290        300 
VFEKFQPYIN WSIDKLCEHF SINKGEKGLN YRIASAILNL KGKTTKSKPF PEVEEFEKSS 

       310        320        330        340        350        360 
IVVKTVHFNK KNVNKESMSF GAFKFEELAN EEWEDSEGYP SAQWRNFLLE TRFLFFVVKE 

       370        380        390        400        410        420 
DEDGVDIFKG IKFFSMPEED INGPVKRMWD DTVKKLKEGV TLEAVPDKST KDGWRIKNNF 

       430        440        450        460        470        480 
VDKSDDLICH VRPHTNNRDY RGGSNADKLP KKINWINRPD SDDYSDEWMT KQSFWINNDY 


IKKQVEDLL 

« Hide

References

[1]"Cloning, expression and characterization of the Sau3AI restriction and modification genes in Staphylococcus carnosus TM300."
Seeber S., Kessler C., Goetz F.
Gene 94:37-43(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 49834 / 3A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M32470 Genomic DNA. Translation: AAA26672.1.
PIRJQ0759.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2REUX-ray1.90A233-489[»]
ProteinModelPortalP16667.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1604. Sau3AI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.600.10. 2 hits.
InterProIPR011337. DNA_rep_MutH/RE_typeII.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PfamPF02976. MutH. 1 hit.
[Graphical view]
SMARTSM00927. MutH. 1 hit.
[Graphical view]
SUPFAMSSF52980. SSF52980. 2 hits.
ProtoNetSearch...

Other

EvolutionaryTraceP16667.

Entry information

Entry nameT2S3_STAAU
AccessionPrimary (citable) accession number: P16667
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references