Reviewed, UniProtKB/Swiss-Prot P16667 (T2S3_STAAU)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme Sau3AI
      Short name=R.Sau3AI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme Sau3AI
    Endonuclease Sau3AI

Gene names

Name:

sau3AIR

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GATC and cleaves before G-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 489

489

Type-2 restriction enzyme Sau3AI

PRO_0000077358

Secondary structure

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489

Helix Strand Turn

Details...

Helix

237 – 245

9

Helix

246 – 248

3

Helix

253 – 259

7

Helix

270 – 278

9

Beta strand

301 – 308

8

Helix

325 – 329

5

Helix

343 – 350

8

Beta strand

352 – 360

9

Beta strand

366 – 368

3

Beta strand

372 – 374

3

Helix

378 – 381

4

Helix

385 – 397

13

Beta strand

401 – 405

5

Beta strand

415 – 418

4

Helix

423 – 425

3

Beta strand

427 – 436

10

Beta strand

446 – 448

3

Beta strand

455 – 457

3

Beta strand

466 – 470

5

Beta strand

472 – 476

5

Helix

478 – 485

8

Helix

486 – 488

3

Sequences

	Sequence		Length	Mass (Da)
	P16667-1 [UniParc]. Last modified August 1, 1990. Version 1. Checksum: FFC3762A949B86EB	FASTA	489	56,471
	10 20 30 40 50 60 MESYLTKQAV HNRAKEAVGK SVLELNGGES IKQSKSSVGD AFENWFGKKK DSDSKPDMAE 70 80 90 100 110 120 AGVELKATPF KKLKNGKYSS KERLVLNIIN YEKVANENFE TSSFLSKNNT IELAFYEYIK 130 140 150 160 170 180 GTPSDNWIIK EAVLYEMHKN PIDYEIIKQD WEIINQYINE GKAHELSEGL TSYLAPCTKG 190 200 210 220 230 240 ANASSLRNQP YSDIKAKQRA FSLKSGYMTS ILRKYVLGDE KIDSIVKDPF EIKEKSIEDI 250 260 270 280 290 300 VFEKFQPYIN WSIDKLCEHF SINKGEKGLN YRIASAILNL KGKTTKSKPF PEVEEFEKSS 310 320 330 340 350 360 IVVKTVHFNK KNVNKESMSF GAFKFEELAN EEWEDSEGYP SAQWRNFLLE TRFLFFVVKE 370 380 390 400 410 420 DEDGVDIFKG IKFFSMPEED INGPVKRMWD DTVKKLKEGV TLEAVPDKST KDGWRIKNNF 430 440 450 460 470 480 VDKSDDLICH VRPHTNNRDY RGGSNADKLP KKINWINRPD SDDYSDEWMT KQSFWINNDY IKKQVEDLL

References

[1]	"Cloning, expression and characterization of the Sau3AI restriction and modification genes in Staphylococcus carnosus TM300." Seeber S., Kessler C., Goetz F. Gene 94:37-43(1990) [PubMed: 2227451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49834 / 3A.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M32470 Genomic DNA. Translation: AAA26672.1.

PIR

JQ0759.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2REU	X-ray	1.90	A	233-489	[»]

ModBase

Search...

Protein family/group databases

REBASE

1604. Sau3AI.

Enzyme and pathway databases

BRENDA

3.1.21.4. 95.

Family and domain databases

InterPro

IPR018140. DNA_mismatch_repair_MutH_cons.
IPR011337. Restrict_endonuc_II/DNA_repair.
[Graphical view]

Gene3D

G3DSA:3.40.600.10. Restrict_endonuc_II/DNA_repair. 1 hit.

Pfam

PF02976. MutH. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

T2S3_STAAU

Accession

Primary (citable) accession number: P16667

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1990
Last sequence update:	August 1, 1990
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)