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P16662 (UD2B7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glucuronosyltransferase 2B7

Short name=UDPGT 2B7
EC=2.4.1.17
Alternative name(s):
3,4-catechol estrogen-specific UDPGT
UDP-glucuronosyltransferase 2B9
Short name=UDPGT 2B9
UDPGTh-2
Gene names
Name:UGT2B7
Synonyms:UGTB2B9
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. Ref.7

Its unique specificity for 3,4-catechol estrogens and estriol suggests it may play an important role in regulating the level and activity of these potent and active estrogen metabolites. Is also active with androsterone, hyodeoxycholic acid and tetrachlorocatechol (in vitro). Ref.7

Catalytic activity

UDP-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside. Ref.7

Subcellular location

Microsome membrane; Single-pass membrane protein Potential. Endoplasmic reticulum membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 529506UDP-glucuronosyltransferase 2B7
PRO_0000036031

Regions

Transmembrane493 – 50917Helical; Potential
Nucleotide binding373 – 3797UDP-glucuronic acid Probable

Sites

Binding site3981UDP-glucuronic acid Probable

Amino acid modifications

Glycosylation671N-linked (GlcNAc...) Potential
Glycosylation681N-linked (GlcNAc...) Ref.6
Glycosylation3151N-linked (GlcNAc...) Potential

Natural variations

Natural variant711A → S.
Corresponds to variant rs12233719 [ dbSNP | Ensembl ].
VAR_057327
Natural variant2681H → Y in allele UGT2B7*2. Ref.2 Ref.3 Ref.4 Ref.5 Ref.8
Corresponds to variant rs7439366 [ dbSNP | Ensembl ].
VAR_012342
Natural variant3781N → S.
Corresponds to variant rs35590824 [ dbSNP | Ensembl ].
VAR_057328

Experimental info

Mutagenesis151S → A: Almost abolishes enzyme activity. Ref.7
Mutagenesis351H → A: Almost abolishes enzyme activity. Ref.7
Mutagenesis1511D → A: Almost abolishes enzyme activity. Ref.7
Mutagenesis3731T → V: Almost abolishes enzyme activity. Ref.7
Mutagenesis3741H → A: Almost abolishes enzyme activity. Ref.7
Mutagenesis3781N → A: Strongly reduced enzyme activity. Ref.7
Mutagenesis3791G → D: Almost abolishes enzyme activity. Ref.7
Mutagenesis3981D → A or N: Almost abolishes enzyme activity. Ref.7
Mutagenesis3991Q → A: Abolishes enzyme activity. Ref.7

Secondary structure

.............................. 529
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P16662 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 94B1CA6CE92F1446

FASTA52960,695
        10         20         30         40         50         60 
MSVKWTSVIL LIQLSFCFSS GNCGKVLVWA AEYSHWMNIK TILDELIQRG HEVTVLASSA 

        70         80         90        100        110        120 
SILFDPNNSS ALKIEIYPTS LTKTELENFI MQQIKRWSDL PKDTFWLYFS QVQEIMSIFG 

       130        140        150        160        170        180 
DITRKFCKDV VSNKKFMKKV QESRFDVIFA DAIFPCSELL AELFNIPFVY SLSFSPGYTF 

       190        200        210        220        230        240 
EKHSGGFIFP PSYVPVVMSE LTDQMTFMER VKNMIYVLYF DFWFEIFDMK KWDQFYSEVL 

       250        260        270        280        290        300 
GRPTTLSETM GKADVWLIRN SWNFQFPHPL LPNVDFVGGL HCKPAKPLPK EMEDFVQSSG 

       310        320        330        340        350        360 
ENGVVVFSLG SMVSNMTEER ANVIASALAQ IPQKVLWRFD GNKPDTLGLN TRLYKWIPQN 

       370        380        390        400        410        420 
DLLGHPKTRA FITHGGANGI YEAIYHGIPM VGIPLFADQP DNIAHMKARG AAVRVDFNTM 

       430        440        450        460        470        480 
SSTDLLNALK RVINDPSYKE NVMKLSRIQH DQPVKPLDRA VFWIEFVMRH KGAKHLRVAA 

       490        500        510        520 
HDLTWFQYHS LDVIGFLLVC VATVIFIVTK CCLFCFWKFA RKAKKGKND 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of human liver UDP-glucuronosyltransferase in COS-1 cells. 3,4-catechol estrogens and estriol as primary substrates."
Ritter J.K., Sheen Y.Y., Owens I.S.
J. Biol. Chem. 265:7900-7906(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT TYR-268.
Tissue: Kidney.
[3]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT TYR-268.
Tissue: Kidney.
[4]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT TYR-268.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT TYR-268.
Tissue: Kidney.
[6]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-68.
Tissue: Liver.
[7]"Crystal structure of the cofactor-binding domain of the human phase II drug-metabolism enzyme UDP-glucuronosyltransferase 2B7."
Miley M.J., Zielinska A.K., Keenan J.E., Bratton S.M., Radominska-Pandya A., Redinbo M.R.
J. Mol. Biol. 369:498-511(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 285-451, CATALYTIC ACTIVITY, FUNCTION TOWARDS STEROIDS, MUTAGENESIS OF SER-15; HIS-35; ASP-151; THR-373; HIS-374; ASN-378; GLY-379; ASP-398 AND GLN-399.
[8]"Genetic polymorphism of UDP-glucuronosyltransferase 2B7 (UGT2B7) at amino acid 268: ethnic diversity of alleles and potential clinical significance."
Bhasker C.R., McKinnon W., Stone A., Lo A.C., Kubota T., Ishizaki T., Miners J.O.
Pharmacogenetics 10:679-685(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT UGT2B7*2 TYR-268.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05428 mRNA. Translation: AAA36793.1.
AK313190 mRNA. Translation: BAG36007.1.
AK223142 mRNA. Translation: BAD96862.1.
AC111000 Genomic DNA. Translation: AAY41045.1.
BC030974 mRNA. Translation: AAH30974.1.
CCDSCCDS3526.1.
PIRA35366.
RefSeqNP_001065.2. NM_001074.2.
UniGeneHs.654424.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2O6LX-ray1.80A/B285-451[»]
ProteinModelPortalP16662.
SMRP16662. Positions 285-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113211. 1 interaction.
IntActP16662. 1 interaction.
STRING9606.ENSP00000304811.

Chemistry

BindingDBP16662.
ChEMBLCHEMBL4370.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

PTM databases

PhosphoSiteP16662.

Polymorphism databases

DMDM136727.

Proteomic databases

MaxQBP16662.
PaxDbP16662.
PRIDEP16662.

Protocols and materials databases

DNASU7364.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000305231; ENSP00000304811; ENSG00000171234.
GeneID7364.
KEGGhsa:7364.
UCSCuc003heg.4. human.

Organism-specific databases

CTD7364.
GeneCardsGC04P069917.
HGNCHGNC:12554. UGT2B7.
MIM600068. gene.
neXtProtNX_P16662.
PharmGKBPA361.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1819.
HOGENOMHOG000220831.
HOVERGENHBG004033.
InParanoidP16662.
KOK00699.
PhylomeDBP16662.
TreeFamTF315472.

Enzyme and pathway databases

BioCycMetaCyc:HS10272-MONOMER.
BRENDA2.4.1.17. 2681.
ReactomeREACT_111217. Metabolism.
SABIO-RKP16662.

Gene expression databases

BgeeP16662.
CleanExHS_UGT2B7.
GenevestigatorP16662.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP16662.
GeneWikiUGT2B7.
GenomeRNAi7364.
NextBio28832.
PROP16662.
SOURCESearch...

Entry information

Entry nameUD2B7_HUMAN
AccessionPrimary (citable) accession number: P16662
Secondary accession number(s): B2R810, Q6GTW0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: July 9, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM