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P16661

- ALG1_YEAST

UniProt

P16661 - ALG1_YEAST

Protein

Chitobiosyldiphosphodolichol beta-mannosyltransferase

Gene

ALG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Aug 1990)
      Previous versions | rss
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    Functioni

    Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER.

    Catalytic activityi

    GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.

    Pathwayi

    GO - Molecular functioni

    1. beta-1,4-mannosyltransferase activity Source: SGD
    2. chitobiosyldiphosphodolichol beta-mannosyltransferase activity Source: UniProtKB-EC
    3. protein binding Source: IntAct

    GO - Biological processi

    1. mannosylation Source: GOC
    2. oligosaccharide-lipid intermediate biosynthetic process Source: SGD
    3. protein N-linked glycosylation Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-7182.
    YEAST:YBR110W-MONOMER.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT33. Glycosyltransferase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chitobiosyldiphosphodolichol beta-mannosyltransferase (EC:2.4.1.142)
    Alternative name(s):
    Asparagine-linked glycosylation protein 1
    Beta-1,4-mannosyltransferase
    GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
    GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
    Gene namesi
    Name:ALG1
    Ordered Locus Names:YBR110W
    ORF Names:YBR0906
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR110w.
    SGDiS000000314. ALG1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi278 – 2781E → K: Abolishes enzymatic activity. 1 Publication
    Mutagenesisi310 – 3101G → D: Abolishes enzymatic activity. 1 Publication
    Mutagenesisi356 – 3561H → Q: Abolishes enzymatic activity. 1 Publication
    Mutagenesisi363 – 3631D → A: No effect on enzymatic activity. 1 Publication
    Mutagenesisi370 – 3701D → A: No effect on enzymatic activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 449449Chitobiosyldiphosphodolichol beta-mannosyltransferasePRO_0000080259Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP16661.
    PaxDbiP16661.
    PeptideAtlasiP16661.

    Expressioni

    Gene expression databases

    GenevestigatoriP16661.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with ALG2 and ALG11.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ALG11P539542EBI-2206309,EBI-2497
    ALG2P436362EBI-2206309,EBI-2459

    Protein-protein interaction databases

    BioGridi32814. 93 interactions.
    DIPiDIP-7371N.
    IntActiP16661. 15 interactions.
    MINTiMINT-1355815.
    STRINGi4932.YBR110W.

    Structurei

    3D structure databases

    ProteinModelPortaliP16661.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini35 – 449415LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 3427Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni435 – 44915Required for oligomerizationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi21 – 3212Dolichol recognitionSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00390000008647.
    HOGENOMiHOG000216716.
    KOiK03842.
    OMAiIVGYKET.
    OrthoDBiEOG72892M.

    Family and domain databases

    InterProiIPR026051. ALG1-like.
    IPR001296. Glyco_trans_1.
    [Graphical view]
    PANTHERiPTHR13036. PTHR13036. 1 hit.
    PfamiPF00534. Glycos_transf_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P16661-1 [UniParc]FASTAAdd to Basket

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    MFLEIPRWLL ALIILYLSIP LVVYYVIPYL FYGNKSTKKR IIIFVLGDVG    50
    HSPRICYHAI SFSKLGWQVE LCGYVEDTLP KIISSDPNIT VHHMSNLKRK 100
    GGGTSVIFMV KKVLFQVLSI FKLLWELRGS DYILVQNPPS IPILPIAVLY 150
    KLTGCKLIID WHNLAYSILQ LKFKGNFYHP LVLISYMVEM IFSKFADYNL 200
    TVTEAMRKYL IQSFHLNPKR CAVLYDRPAS QFQPLAGDIS RQKALTTKAF 250
    IKNYIRDDFD TEKGDKIIVT STSFTPDEDI GILLGALKIY ENSYVKFDSS 300
    LPKILCFITG KGPLKEKYMK QVEEYDWKRC QIEFVWLSAE DYPKLLQLCD 350
    YGVSLHTSSS GLDLPMKILD MFGSGLPVIA MNYPVLDELV QHNVNGLKFV 400
    DRRELHESLI FAMKDADLYQ KLKKNVTQEA ENRWQSNWER TMRDLKLIH 449
    Length:449
    Mass (Da):51,929
    Last modified:August 1, 1990 - v1
    Checksum:i662E0CB1632DED5A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05416 Genomic DNA. Translation: AAA66322.1.
    X78993 Genomic DNA. Translation: CAA55613.1.
    Z35979 Genomic DNA. Translation: CAA85067.1.
    BK006936 Genomic DNA. Translation: DAA07229.1.
    PIRiA35762.
    RefSeqiNP_009668.3. NM_001178458.3.

    Genome annotation databases

    EnsemblFungiiYBR110W; YBR110W; YBR110W.
    GeneIDi852407.
    KEGGisce:YBR110W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05416 Genomic DNA. Translation: AAA66322.1 .
    X78993 Genomic DNA. Translation: CAA55613.1 .
    Z35979 Genomic DNA. Translation: CAA85067.1 .
    BK006936 Genomic DNA. Translation: DAA07229.1 .
    PIRi A35762.
    RefSeqi NP_009668.3. NM_001178458.3.

    3D structure databases

    ProteinModelPortali P16661.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32814. 93 interactions.
    DIPi DIP-7371N.
    IntActi P16661. 15 interactions.
    MINTi MINT-1355815.
    STRINGi 4932.YBR110W.

    Protein family/group databases

    CAZyi GT33. Glycosyltransferase Family 33.

    Proteomic databases

    MaxQBi P16661.
    PaxDbi P16661.
    PeptideAtlasi P16661.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR110W ; YBR110W ; YBR110W .
    GeneIDi 852407.
    KEGGi sce:YBR110W.

    Organism-specific databases

    CYGDi YBR110w.
    SGDi S000000314. ALG1.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00390000008647.
    HOGENOMi HOG000216716.
    KOi K03842.
    OMAi IVGYKET.
    OrthoDBi EOG72892M.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BioCyci MetaCyc:MONOMER-7182.
    YEAST:YBR110W-MONOMER.

    Miscellaneous databases

    NextBioi 971252.
    PROi P16661.

    Gene expression databases

    Genevestigatori P16661.

    Family and domain databases

    InterProi IPR026051. ALG1-like.
    IPR001296. Glyco_trans_1.
    [Graphical view ]
    PANTHERi PTHR13036. PTHR13036. 1 hit.
    Pfami PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence and transcript heterogeneity of the yeast gene ALG1, an essential mannosyltransferase involved in N-glycosylation."
      Albright C.F., Robbins P.W.
      J. Biol. Chem. 265:7042-7049(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum."
      Gao X.-D., Nishikawa A., Dean N.
      Glycobiology 14:559-570(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: OLIGOMERIZATION, INTERACTION WITH ALG2 AND ALG11, MUTAGENESIS OF GLU-278; GLY-310; HIS-356; ASP-363 AND ASP-370.

    Entry informationi

    Entry nameiALG1_YEAST
    AccessioniPrimary (citable) accession number: P16661
    Secondary accession number(s): D6VQA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1990
    Last sequence update: August 1, 1990
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1890 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3