Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chitobiosyldiphosphodolichol beta-mannosyltransferase

Gene

ALG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc2-Man5 intermediate on the cytoplasmic surface of the ER.

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  • protein N-linked glycosylation Source: SGD

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YBR110W-MONOMER
YEAST:YBR110W-MONOMER
ReactomeiR-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT33 Glycosyltransferase Family 33

Names & Taxonomyi

Protein namesi
Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase (EC:2.4.1.142)
Alternative name(s):
Asparagine-linked glycosylation protein 1
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
Gene namesi
Name:ALG1
Ordered Locus Names:YBR110W
ORF Names:YBR0906
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR110W
SGDiS000000314 ALG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 34Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST27
Topological domaini35 – 449LumenalSequence analysisAdd BLAST415

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi278E → K: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi310G → D: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi356H → Q: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi363D → A: No effect on enzymatic activity. 1 Publication1
Mutagenesisi370D → A: No effect on enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802591 – 449Chitobiosyldiphosphodolichol beta-mannosyltransferaseAdd BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi88N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi199N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi425N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP16661
PaxDbiP16661
PRIDEiP16661

Interactioni

Subunit structurei

Homodimer. Interacts with ALG2 and ALG11.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32814, 360 interactors
DIPiDIP-7371N
IntActiP16661, 15 interactors
MINTiP16661
STRINGi4932.YBR110W

Structurei

3D structure databases

ProteinModelPortaliP16661
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni435 – 449Required for oligomerizationAdd BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 32Dolichol recognitionSequence analysisAdd BLAST12

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000008647
HOGENOMiHOG000216716
InParanoidiP16661
KOiK03842
OMAiGPQKEHY
OrthoDBiEOG092C312J

Family and domain databases

InterProiView protein in InterPro
IPR026051 ALG1-like
IPR001296 Glyco_trans_1
PANTHERiPTHR13036 PTHR13036, 2 hits
PfamiView protein in Pfam
PF00534 Glycos_transf_1, 1 hit

Sequencei

Sequence statusi: Complete.

P16661-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLEIPRWLL ALIILYLSIP LVVYYVIPYL FYGNKSTKKR IIIFVLGDVG
60 70 80 90 100
HSPRICYHAI SFSKLGWQVE LCGYVEDTLP KIISSDPNIT VHHMSNLKRK
110 120 130 140 150
GGGTSVIFMV KKVLFQVLSI FKLLWELRGS DYILVQNPPS IPILPIAVLY
160 170 180 190 200
KLTGCKLIID WHNLAYSILQ LKFKGNFYHP LVLISYMVEM IFSKFADYNL
210 220 230 240 250
TVTEAMRKYL IQSFHLNPKR CAVLYDRPAS QFQPLAGDIS RQKALTTKAF
260 270 280 290 300
IKNYIRDDFD TEKGDKIIVT STSFTPDEDI GILLGALKIY ENSYVKFDSS
310 320 330 340 350
LPKILCFITG KGPLKEKYMK QVEEYDWKRC QIEFVWLSAE DYPKLLQLCD
360 370 380 390 400
YGVSLHTSSS GLDLPMKILD MFGSGLPVIA MNYPVLDELV QHNVNGLKFV
410 420 430 440
DRRELHESLI FAMKDADLYQ KLKKNVTQEA ENRWQSNWER TMRDLKLIH
Length:449
Mass (Da):51,929
Last modified:August 1, 1990 - v1
Checksum:i662E0CB1632DED5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05416 Genomic DNA Translation: AAA66322.1
X78993 Genomic DNA Translation: CAA55613.1
Z35979 Genomic DNA Translation: CAA85067.1
BK006936 Genomic DNA Translation: DAA07229.1
PIRiA35762
RefSeqiNP_009668.3, NM_001178458.3

Genome annotation databases

EnsemblFungiiYBR110W; YBR110W; YBR110W
GeneIDi852407
KEGGisce:YBR110W

Similar proteinsi

Entry informationi

Entry nameiALG1_YEAST
AccessioniPrimary (citable) accession number: P16661
Secondary accession number(s): D6VQA9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health