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P16661 (ALG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase

EC=2.4.1.142
Alternative name(s):
Asparagine-linked glycosylation protein 1
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
Gene names
Name:ALG1
Ordered Locus Names:YBR110W
ORF Names:YBR0906
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER.

Catalytic activity

GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Homodimer. Interacts with ALG2 and ALG11. Ref.7

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein Potential Ref.5.

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ALG11P539542EBI-2206309,EBI-2497
ALG2P436362EBI-2206309,EBI-2459

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Chitobiosyldiphosphodolichol beta-mannosyltransferase
PRO_0000080259

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 3427Helical; Signal-anchor for type II membrane protein; Potential
Topological domain35 – 449415Lumenal Potential
Region435 – 44915Required for oligomerization
Motif21 – 3212Dolichol recognition Potential

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation1991N-linked (GlcNAc...) Potential
Glycosylation4251N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis2781E → K: Abolishes enzymatic activity. Ref.7
Mutagenesis3101G → D: Abolishes enzymatic activity. Ref.7
Mutagenesis3561H → Q: Abolishes enzymatic activity. Ref.7
Mutagenesis3631D → A: No effect on enzymatic activity. Ref.7
Mutagenesis3701D → A: No effect on enzymatic activity. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P16661 [UniParc].

Last modified August 1, 1990. Version 1.
Checksum: 662E0CB1632DED5A

FASTA44951,929
        10         20         30         40         50         60 
MFLEIPRWLL ALIILYLSIP LVVYYVIPYL FYGNKSTKKR IIIFVLGDVG HSPRICYHAI 

        70         80         90        100        110        120 
SFSKLGWQVE LCGYVEDTLP KIISSDPNIT VHHMSNLKRK GGGTSVIFMV KKVLFQVLSI 

       130        140        150        160        170        180 
FKLLWELRGS DYILVQNPPS IPILPIAVLY KLTGCKLIID WHNLAYSILQ LKFKGNFYHP 

       190        200        210        220        230        240 
LVLISYMVEM IFSKFADYNL TVTEAMRKYL IQSFHLNPKR CAVLYDRPAS QFQPLAGDIS 

       250        260        270        280        290        300 
RQKALTTKAF IKNYIRDDFD TEKGDKIIVT STSFTPDEDI GILLGALKIY ENSYVKFDSS 

       310        320        330        340        350        360 
LPKILCFITG KGPLKEKYMK QVEEYDWKRC QIEFVWLSAE DYPKLLQLCD YGVSLHTSSS 

       370        380        390        400        410        420 
GLDLPMKILD MFGSGLPVIA MNYPVLDELV QHNVNGLKFV DRRELHESLI FAMKDADLYQ 

       430        440 
KLKKNVTQEA ENRWQSNWER TMRDLKLIH 

« Hide

References

« Hide 'large scale' references
[1]"The sequence and transcript heterogeneity of the yeast gene ALG1, an essential mannosyltransferase involved in N-glycosylation."
Albright C.F., Robbins P.W.
J. Biol. Chem. 265:7042-7049(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of a 70 kb region on the right arm of yeast chromosome II."
Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum."
Gao X.-D., Nishikawa A., Dean N.
Glycobiology 14:559-570(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: OLIGOMERIZATION, INTERACTION WITH ALG2 AND ALG11, MUTAGENESIS OF GLU-278; GLY-310; HIS-356; ASP-363 AND ASP-370.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05416 Genomic DNA. Translation: AAA66322.1.
X78993 Genomic DNA. Translation: CAA55613.1.
Z35979 Genomic DNA. Translation: CAA85067.1.
BK006936 Genomic DNA. Translation: DAA07229.1.
PIRA35762.
RefSeqNP_009668.3. NM_001178458.3.

3D structure databases

ProteinModelPortalP16661.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32814. 93 interactions.
DIPDIP-7371N.
IntActP16661. 15 interactions.
MINTMINT-1355815.
STRING4932.YBR110W.

Protein family/group databases

CAZyGT33. Glycosyltransferase Family 33.

Proteomic databases

PaxDbP16661.
PeptideAtlasP16661.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR110W; YBR110W; YBR110W.
GeneID852407.
KEGGsce:YBR110W.

Organism-specific databases

CYGDYBR110w.
SGDS000000314. ALG1.

Phylogenomic databases

eggNOGCOG0438.
GeneTreeENSGT00390000008647.
HOGENOMHOG000216716.
KOK03842.
OMADIVGYKE.
OrthoDBEOG72892M.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-7182.
YEAST:YBR110W-MONOMER.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorP16661.

Family and domain databases

InterProIPR026051. ALG1-like.
IPR001296. Glyco_trans_1.
[Graphical view]
PANTHERPTHR13036. PTHR13036. 1 hit.
PfamPF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971252.
PROP16661.

Entry information

Entry nameALG1_YEAST
AccessionPrimary (citable) accession number: P16661
Secondary accession number(s): D6VQA9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: April 16, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways