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P16661

- ALG1_YEAST

UniProt

P16661 - ALG1_YEAST

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Protein

Chitobiosyldiphosphodolichol beta-mannosyltransferase

Gene

ALG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER.

Catalytic activityi

GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.

Pathwayi

GO - Molecular functioni

  1. beta-1,4-mannosyltransferase activity Source: SGD
  2. chitobiosyldiphosphodolichol beta-mannosyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. mannosylation Source: GOC
  2. oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  3. protein N-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7182.
YEAST:YBR110W-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT33. Glycosyltransferase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase (EC:2.4.1.142)
Alternative name(s):
Asparagine-linked glycosylation protein 1
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase
Gene namesi
Name:ALG1
Ordered Locus Names:YBR110W
ORF Names:YBR0906
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR110w.
SGDiS000000314. ALG1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. integral component of membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi278 – 2781E → K: Abolishes enzymatic activity. 1 Publication
Mutagenesisi310 – 3101G → D: Abolishes enzymatic activity. 1 Publication
Mutagenesisi356 – 3561H → Q: Abolishes enzymatic activity. 1 Publication
Mutagenesisi363 – 3631D → A: No effect on enzymatic activity. 1 Publication
Mutagenesisi370 – 3701D → A: No effect on enzymatic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Chitobiosyldiphosphodolichol beta-mannosyltransferasePRO_0000080259Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP16661.
PaxDbiP16661.
PeptideAtlasiP16661.

Expressioni

Gene expression databases

GenevestigatoriP16661.

Interactioni

Subunit structurei

Homodimer. Interacts with ALG2 and ALG11.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ALG11P539542EBI-2206309,EBI-2497
ALG2P436362EBI-2206309,EBI-2459

Protein-protein interaction databases

BioGridi32814. 93 interactions.
DIPiDIP-7371N.
IntActiP16661. 15 interactions.
MINTiMINT-1355815.
STRINGi4932.YBR110W.

Structurei

3D structure databases

ProteinModelPortaliP16661.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Topological domaini35 – 449415LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 3427Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni435 – 44915Required for oligomerizationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi21 – 3212Dolichol recognitionSequence AnalysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000008647.
HOGENOMiHOG000216716.
InParanoidiP16661.
KOiK03842.
OMAiIVGYKET.
OrthoDBiEOG72892M.

Family and domain databases

InterProiIPR026051. ALG1-like.
IPR001296. Glyco_trans_1.
[Graphical view]
PANTHERiPTHR13036. PTHR13036. 1 hit.
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P16661-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFLEIPRWLL ALIILYLSIP LVVYYVIPYL FYGNKSTKKR IIIFVLGDVG
60 70 80 90 100
HSPRICYHAI SFSKLGWQVE LCGYVEDTLP KIISSDPNIT VHHMSNLKRK
110 120 130 140 150
GGGTSVIFMV KKVLFQVLSI FKLLWELRGS DYILVQNPPS IPILPIAVLY
160 170 180 190 200
KLTGCKLIID WHNLAYSILQ LKFKGNFYHP LVLISYMVEM IFSKFADYNL
210 220 230 240 250
TVTEAMRKYL IQSFHLNPKR CAVLYDRPAS QFQPLAGDIS RQKALTTKAF
260 270 280 290 300
IKNYIRDDFD TEKGDKIIVT STSFTPDEDI GILLGALKIY ENSYVKFDSS
310 320 330 340 350
LPKILCFITG KGPLKEKYMK QVEEYDWKRC QIEFVWLSAE DYPKLLQLCD
360 370 380 390 400
YGVSLHTSSS GLDLPMKILD MFGSGLPVIA MNYPVLDELV QHNVNGLKFV
410 420 430 440
DRRELHESLI FAMKDADLYQ KLKKNVTQEA ENRWQSNWER TMRDLKLIH
Length:449
Mass (Da):51,929
Last modified:August 1, 1990 - v1
Checksum:i662E0CB1632DED5A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05416 Genomic DNA. Translation: AAA66322.1.
X78993 Genomic DNA. Translation: CAA55613.1.
Z35979 Genomic DNA. Translation: CAA85067.1.
BK006936 Genomic DNA. Translation: DAA07229.1.
PIRiA35762.
RefSeqiNP_009668.3. NM_001178458.3.

Genome annotation databases

EnsemblFungiiYBR110W; YBR110W; YBR110W.
GeneIDi852407.
KEGGisce:YBR110W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05416 Genomic DNA. Translation: AAA66322.1 .
X78993 Genomic DNA. Translation: CAA55613.1 .
Z35979 Genomic DNA. Translation: CAA85067.1 .
BK006936 Genomic DNA. Translation: DAA07229.1 .
PIRi A35762.
RefSeqi NP_009668.3. NM_001178458.3.

3D structure databases

ProteinModelPortali P16661.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32814. 93 interactions.
DIPi DIP-7371N.
IntActi P16661. 15 interactions.
MINTi MINT-1355815.
STRINGi 4932.YBR110W.

Protein family/group databases

CAZyi GT33. Glycosyltransferase Family 33.

Proteomic databases

MaxQBi P16661.
PaxDbi P16661.
PeptideAtlasi P16661.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR110W ; YBR110W ; YBR110W .
GeneIDi 852407.
KEGGi sce:YBR110W.

Organism-specific databases

CYGDi YBR110w.
SGDi S000000314. ALG1.

Phylogenomic databases

eggNOGi COG0438.
GeneTreei ENSGT00390000008647.
HOGENOMi HOG000216716.
InParanoidi P16661.
KOi K03842.
OMAi IVGYKET.
OrthoDBi EOG72892M.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci MetaCyc:MONOMER-7182.
YEAST:YBR110W-MONOMER.

Miscellaneous databases

NextBioi 971252.
PROi P16661.

Gene expression databases

Genevestigatori P16661.

Family and domain databases

InterProi IPR026051. ALG1-like.
IPR001296. Glyco_trans_1.
[Graphical view ]
PANTHERi PTHR13036. PTHR13036. 1 hit.
Pfami PF00534. Glycos_transf_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence and transcript heterogeneity of the yeast gene ALG1, an essential mannosyltransferase involved in N-glycosylation."
    Albright C.F., Robbins P.W.
    J. Biol. Chem. 265:7042-7049(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
    Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
    Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum."
    Gao X.-D., Nishikawa A., Dean N.
    Glycobiology 14:559-570(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: OLIGOMERIZATION, INTERACTION WITH ALG2 AND ALG11, MUTAGENESIS OF GLU-278; GLY-310; HIS-356; ASP-363 AND ASP-370.

Entry informationi

Entry nameiALG1_YEAST
AccessioniPrimary (citable) accession number: P16661
Secondary accession number(s): D6VQA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1990
Last sequence update: August 1, 1990
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3